B1WZW5 (BIOD_CYAA5) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
ATP-dependent dethiobiotin synthetase BioD
EC=6.3.3.3

Alternative name(s):
DTB synthetase
Short name=DTBS
Dethiobiotin synthase

Gene names

Name:	bioD
Ordered Locus Names:	cce_3516

Organism

Cyanothece sp. (strain ATCC 51142) [Complete proteome] [HAMAP]

Taxonomic identifier

43989 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Chroococcales › Cyanothece

Protein attributes

Sequence length	221 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes a mechanistically unusual reaction, the ATP-dependent insertion of CO2 between the N7 and N8 nitrogen atoms of 7,8-diaminopelargonic acid (DAPA) to form an ureido ring By similarity. HAMAP MF_00336
Catalytic activity	ATP + 7,8-diaminononanoate + CO2 = ADP + phosphate + dethiobiotin. HAMAP MF_00336
Cofactor	Magnesium By similarity. HAMAP MF_00336
Pathway	Cofactor biosynthesis; biotin biosynthesis; biotin from 7,8-diaminononanoate: step 1/2. HAMAP MF_00336
Subcellular location	Cytoplasm By similarity HAMAP MF_00336.
Sequence similarities	Belongs to the dethiobiotin synthetase family.

Ontologies

Keywords
Biological process	Biotin biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	biotin biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW dethiobiotin synthase activity Inferred from electronic annotation. Source: EC magnesium ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 221	221	ATP-dependent dethiobiotin synthetase BioD HAMAP MF_00336		PRO_1000133212
Regions
	Nucleotide binding	12 – 17	6	ATP By similarity
	Nucleotide binding	105 – 108	4	ATP By similarity
	Nucleotide binding	165 – 166	2	ATP By similarity
Sites
	Metal binding	12	1	Magnesium 1 By similarity
	Metal binding	16	1	Magnesium 2 By similarity
	Metal binding	47	1	Magnesium 2 By similarity
	Metal binding	105	1	Magnesium 2 By similarity
	Binding site	43	1	Substrate By similarity
	Binding site	47	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	B1WZW5 [UniParc]. Last modified May 20, 2008. Version 1. Checksum: 1E07CE911A4480B7	FASTA	221	24,103
	10 20 30 40 50 60 MTTLFIVGTD TEVGKTVVTS ALAAYWQTYH SSESLGVIKL LQTGIGDVEH YQRLLPHVEV 70 80 90 100 110 120 VNPLRYETPV APPVAAEKEN RSIPLDQVWQ GLNDLQQRKN LVLAEGLGGL GSPVTWELTV 130 140 150 160 170 180 ADLAGEWGLP TVLVVPVKLG AIAQTVANVA LARQYKVNLK GIIFSCPRPL SHEEIVNFAP 190 200 210 220 ITLIESLTKT PVLGMISYLE NIEDITKLAH IASSLDLEML F

References

[1]

"The genome of Cyanothece 51142, a unicellular diazotrophic cyanobacterium important in the marine nitrogen cycle." Welsh E.A., Liberton M., Stoeckel J., Loh T., Elvitigala T., Wang C., Wollam A., Fulton R.S., Clifton S.W., Jacobs J.M., Aurora R., Ghosh B.K., Sherman L.A., Smith R.D., Wilson R.K., Pakrasi H.B. Proc. Natl. Acad. Sci. U.S.A. 105:15094-15099(2008) [PubMed: 18812508] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 51142.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000806 Genomic DNA. Translation: ACB52864.1.
RefSeq	YP_001804930.1. NC_010546.1.
3D structure databases
ProteinModelPortal	B1WZW5.
ModBase	Search...
Protein-protein interaction databases
STRING	B1WZW5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6170579.
GenomeReviews	Gene locus cce_3516 in contig CP000806_GR.
KEGG	cyt:cce_3516.
PATRIC	21545410. VBICyaSp130209_3521.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG650065.
OMA	ALAAYWQ.
PhylomeDB	B1WZW5.
ProtClustDB	PRK00090.
Family and domain databases
HAMAP	MF_00336. BioD. [Tree]
InterPro	IPR004472. DTB_synth_BioD. [Graphical view]
KO	K01935.
PIRSF	PIRSF006755. DTB_synth. 1 hit.
TIGRFAMs	TIGR00347. BioD. 1 hit.
ProtoNet	Search...

Entry information

Entry name

BIOD_CYAA5

Accession

Primary (citable) accession number: B1WZW5

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 28, 2009
Last sequence update:	May 20, 2008
Last modified:	January 25, 2012
This is version 32 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program