B1WZH9 (AROA_CYAA5) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
3-phosphoshikimate 1-carboxyvinyltransferase
EC=2.5.1.19

Alternative name(s):
5-enolpyruvylshikimate-3-phosphate synthase
Short name=EPSP synthase
Short name=EPSPS

Gene names

Name:	aroA
Ordered Locus Names:	cce_1781

Organism

Cyanothece sp. (strain ATCC 51142) [Complete proteome] [HAMAP]

Taxonomic identifier

43989 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Chroococcales › Cyanothece

Protein attributes

Sequence length	451 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Phosphoenolpyruvate + 3-phosphoshikimate = phosphate + 5-O-(1-carboxyvinyl)-3-phosphoshikimate. HAMAP MF_00210
Pathway	Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 6/7. HAMAP MF_00210
Subunit structure	Monomer By similarity. HAMAP MF_00210
Subcellular location	Cytoplasm By similarity HAMAP MF_00210.
Sequence similarities	Belongs to the EPSP synthase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Aromatic amino acid biosynthesis
Cellular component	Cytoplasm
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	aromatic amino acid family biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3-phosphoshikimate 1-carboxyvinyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 451	451	3-phosphoshikimate 1-carboxyvinyltransferase HAMAP MF_00210		PRO_1000099697

Sequences

	Sequence		Length	Mass (Da)
	B1WZH9 [UniParc]. Last modified May 20, 2008. Version 1. Checksum: 1986CE725B371238	FASTA	451	47,683
	10 20 30 40 50 60 MSKSIINLQS VENQQILSIT PPNTGLSLQG TLQIPGDKSI SHRALMLGAI AEGETIIAGL 70 80 90 100 110 120 LLGEDPRSTA ECFRAMGAQI SPLNSEKVTI QGIGLGKLQE PLDVLNAGNS GTTMRLMLGL 130 140 150 160 170 180 LASHPDRLFC VTGDASLRSR PMSRVIQPLQ DMGANIWGRK NNTLAPLAVQ GKSLKPIHYH 190 200 210 220 230 240 SPIASAQVKS CILLAGLMTD GKTTVTEPAL SRDHSERMLQ AFGATLDIDS QTNSVTINGH 250 260 270 280 290 300 PKLIGQTVIV PGDISSSAFW LVAASIVPGS ELLIENVGIN PTRTGILEAL EMMGADITLE 310 320 330 340 350 360 NKRILTGEPV ADLRVKSCQL KGCTIGGNII PRLIDEVPIL AVAGIFATGK TIIKDAAELR 370 380 390 400 410 420 VKESDRLAVM ASELTKMGAK ITELPDGLEI TGGTPLKAAE VDSYTDHRIA MSLAIAALNA 430 440 450 KGKTIINRAE AAAISYPKFV ETLQQVCGDS N

References

[1]

"The genome of Cyanothece 51142, a unicellular diazotrophic cyanobacterium important in the marine nitrogen cycle." Welsh E.A., Liberton M., Stoeckel J., Loh T., Elvitigala T., Wang C., Wollam A., Fulton R.S., Clifton S.W., Jacobs J.M., Aurora R., Ghosh B.K., Sherman L.A., Smith R.D., Wilson R.K., Pakrasi H.B. Proc. Natl. Acad. Sci. U.S.A. 105:15094-15099(2008) [PubMed: 18812508] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 51142.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000806 Genomic DNA. Translation: ACB51131.1.
RefSeq	YP_001803197.1. NC_010546.1.
3D structure databases
ProteinModelPortal	B1WZH9.
ModBase	Search...
Protein-protein interaction databases
STRING	B1WZH9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6168830.
GenomeReviews	Gene locus cce_1781 in contig CP000806_GR.
KEGG	cyt:cce_1781.
PATRIC	21542019. VBICyaSp130209_1839.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG646626.
OMA	GEDPRST.
PhylomeDB	B1WZH9.
ProtClustDB	PRK02427.
Family and domain databases
HAMAP	MF_00210. EPSP_synth. [Tree]
InterPro	IPR001986. Enolpyruvate_Tfrase_dom. IPR006264. EPSP_synthase. IPR023193. EPSP_synthase_CS. IPR013792. RNA3'P_cycl/enolpyr_Trfase_a/b. [Graphical view]
Gene3D	G3DSA:3.65.10.10. EPSP_synthase. 2 hits.
KO	K00800.
Pfam	PF00275. EPSP_synthase. 1 hit. [Graphical view]
PIRSF	PIRSF000505. EPSPS. 1 hit.
SUPFAM	SSF55205. RNA3'_cycl/enolpyr_transf_A/B. 1 hit.
TIGRFAMs	TIGR01356. AroA. 1 hit.
PROSITE	PS00104. EPSP_SYNTHASE_1. 1 hit. PS00885. EPSP_SYNTHASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AROA_CYAA5

Accession

Primary (citable) accession number: B1WZH9

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	May 20, 2008
Last modified:	January 25, 2012
This is version 29 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program