B1WYU1 (SYE_CYAA5) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutamate--tRNA ligase
EC=6.1.1.17

Alternative name(s):
Glutamyl-tRNA synthetase
Short name=GluRS

Gene names

Name:	gltX
Ordered Locus Names:	cce_1758

Organism

Cyanothece sp. (strain ATCC 51142) [Complete proteome] [HAMAP]

Taxonomic identifier

43989 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Chroococcales › Cyanothece

Protein attributes

Sequence length	484 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) By similarity. HAMAP MF_00022_B
Catalytic activity	ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu). HAMAP MF_00022_B
Cofactor	Binds 1 zinc ion per subunit By similarity. HAMAP MF_00022_B
Subunit structure	Monomer By similarity. HAMAP MF_00022_B
Subcellular location	Cytoplasm By similarity HAMAP MF_00022_B.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glutamyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW glutamate-tRNA ligase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 484	484	Glutamate--tRNA ligase HAMAP MF_00022_B		PRO_1000090069
Regions
	Motif	9 – 19	11	"HIGH" region HAMAP MF_00022_B
	Motif	250 – 254	5	"KMSKS" region HAMAP MF_00022_B
Sites
	Metal binding	98	1	Zinc By similarity
	Metal binding	100	1	Zinc By similarity
	Metal binding	125	1	Zinc By similarity
	Metal binding	127	1	Zinc By similarity
	Binding site	253	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	B1WYU1 [UniParc]. Last modified May 20, 2008. Version 1. Checksum: 119C779725BFFF5B	FASTA	484	55,281
	10 20 30 40 50 60 MTVRVRIAPS PTGNLHIGTA RTAVFNWLFA HHHQGQFILR VEDTDRERSR AEYTENIKSG 70 80 90 100 110 120 LAWLGLTWDE GPFFQTQRLD LYRQGIQTLL DKGFAYRCYC TPEELEQMRE AQKAQNQAPR 130 140 150 160 170 180 YDNRHRHLTE EQRQGLEAQG RKPVIRFIID DDREIVWHDL IRGKMTWKGS DLGGDMVIAR 190 200 210 220 230 240 IADNPDQPFG QPLYNLAVVV DDMDMKITHV IRGEDHIANT AKQILLYEAL GATVPEFAHT 250 260 270 280 290 300 PLILNQEGRK LSKRDGVTSI DDFRKMGFLP QALANYMTLL GWTPPDSTQE IFTLTEAAQQ 310 320 330 340 350 360 FSLERVNKAG AKFDWDKLDW INSQYVHKMS GEELVDLLVP YWQEAGYPIN IDSDRPWLEK 370 380 390 400 410 420 MATLIGPSLT RLSDAAKESV LLFGGRVDYS EEAIAQMKQD GVKDVLQAVV EKIQESSQLT 430 440 450 460 470 480 EDEAKDTIKQ VTKTFKVKKG LVMRSLRAGL MGELHGPDLI QSWLLLHEKG WDKTRLTHGL SLVE

References

[1]

"The genome of Cyanothece 51142, a unicellular diazotrophic cyanobacterium important in the marine nitrogen cycle." Welsh E.A., Liberton M., Stoeckel J., Loh T., Elvitigala T., Wang C., Wollam A., Fulton R.S., Clifton S.W., Jacobs J.M., Aurora R., Ghosh B.K., Sherman L.A., Smith R.D., Wilson R.K., Pakrasi H.B. Proc. Natl. Acad. Sci. U.S.A. 105:15094-15099(2008) [PubMed: 18812508] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 51142.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000806 Genomic DNA. Translation: ACB51108.1.
RefSeq	YP_001803174.1. NC_010546.1.
3D structure databases
ProteinModelPortal	B1WYU1.
SMR	B1WYU1. Positions 2-480.
ModBase	Search...
Protein-protein interaction databases
STRING	B1WYU1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6168807.
GenomeReviews	Gene locus cce_1758 in contig CP000806_GR.
KEGG	cyt:cce_1758.
PATRIC	21541973. VBICyaSp130209_1816.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG628189.
OMA	DKETAND.
PhylomeDB	B1WYU1.
ProtClustDB	PRK01406.
Family and domain databases
HAMAP	MF_00022_B. Glu_tRNA_synth_B. [Tree]
InterPro	IPR008925. aa-tRNA-synth_I_codon-bd. IPR020751. aa-tRNA-synth_I_codon-bd_sub2. IPR001412. aa-tRNA-synth_I_CS. IPR004527. Glu-tRNA-synth_Ib_bac/mito. IPR000924. Glu/Gln-tRNA-synth_Ib. IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl. IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:1.10.1160.10. Glu/Gln-tRNA-synth_Ic_a-bdl. 1 hit. G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 2 hits. G3DSA:1.10.10.350. tRNA_synt_bd. 1 hit.
KO	K01885.
PANTHER	PTHR10119. Glu_tRNA-synt_1c. 1 hit. PTHR10119:SF1. PTHR10119:SF1. 1 hit.
Pfam	PF00749. tRNA-synt_1c. 1 hit. [Graphical view]
PRINTS	PR00987. TRNASYNTHGLU.
SUPFAM	SSF48163. tRNA-synt_bind. 1 hit.
TIGRFAMs	TIGR00464. GltX_bact. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYE_CYAA5

Accession

Primary (citable) accession number: B1WYU1

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	May 20, 2008
Last modified:	January 25, 2012
This is version 31 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program