B1WYG5 (GMHA_CYAA5) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphoheptose isomerase
EC=5.3.1.28

Alternative name(s):
Sedoheptulose 7-phosphate isomerase

Gene names

Name:	gmhA
Ordered Locus Names:	cce_0043

Organism

Cyanothece sp. (strain ATCC 51142) [Complete proteome] [HAMAP]

Taxonomic identifier

43989 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Chroococcales › Cyanothece

Protein attributes

Sequence length	196 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the isomerization of sedoheptulose 7-phosphate in D-glycero-D-manno-heptose 7-phosphate By similarity. HAMAP MF_00067
Catalytic activity	D-sedoheptulose 7-phosphate = D-glycero-D-manno-heptose 7-phosphate. HAMAP MF_00067
Cofactor	Binds 1 zinc ion per subunit By similarity. HAMAP MF_00067
Pathway	Carbohydrate biosynthesis; D-glycero-D-manno-heptose 7-phosphate biosynthesis; D-glycero-alpha-D-manno-heptose 7-phosphate and D-glycero-beta-D-manno-heptose 7-phosphate from sedoheptulose 7-phosphate: step 1/1. HAMAP MF_00067
Subcellular location	Cytoplasm By similarity HAMAP MF_00067.
Miscellaneous	The reaction produces a racemic mixture of D-glycero-alpha-D-manno-heptose 7-phosphate and D-glycero-beta-D-manno-heptose 7-phosphate By similarity. HAMAP MF_00067
Sequence similarities	Belongs to the SIS family. GmhA subfamily. Contains 1 SIS domain.

Ontologies

Keywords
Biological process	Carbohydrate metabolism
Cellular component	Cytoplasm
Ligand	Metal-binding Zinc
Molecular function	Isomerase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	lipopolysaccharide core region biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	D-sedoheptulose 7-phosphate isomerase activity Inferred from electronic annotation. Source: InterPro metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW sugar binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 196	196	Phosphoheptose isomerase HAMAP MF_00067		PRO_1000092269
Regions
	Domain	31 – 196	166	SIS
	Region	46 – 48	3	Substrate binding By similarity
	Region	88 – 89	2	Substrate binding By similarity
	Region	114 – 116	3	Substrate binding By similarity
Sites
	Metal binding	55	1	Zinc By similarity
	Metal binding	59	1	Zinc By similarity
	Metal binding	166	1	Zinc By similarity
	Metal binding	174	1	Zinc By similarity
	Binding site	59	1	Substrate By similarity
	Binding site	119	1	Substrate By similarity
	Binding site	166	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	B1WYG5 [UniParc]. Last modified May 20, 2008. Version 1. Checksum: DB512B6BCEA64E3C	FASTA	196	21,641
	10 20 30 40 50 60 MNYWIQHRLN CLEQAFNPKY CRALEHTIGI VARQFKAGNK LLICGNGGSA ADAQHIAAEF 70 80 90 100 110 120 VGRFQLHRKG LPAIALGTNP ATLTAWSNDY EFETVFARQV EAFAQPGDIL WGISTSGKSA 130 140 150 160 170 180 NVIRAMEMAK DLGLLTIGMA GNNGGMLKDL TDYPLFVSEY HTPYIQEIHL ITYHRICEQV 190 EAQLFAKAGL EAQIAV

References

[1]

"The genome of Cyanothece 51142, a unicellular diazotrophic cyanobacterium important in the marine nitrogen cycle." Welsh E.A., Liberton M., Stoeckel J., Loh T., Elvitigala T., Wang C., Wollam A., Fulton R.S., Clifton S.W., Jacobs J.M., Aurora R., Ghosh B.K., Sherman L.A., Smith R.D., Wilson R.K., Pakrasi H.B. Proc. Natl. Acad. Sci. U.S.A. 105:15094-15099(2008) [PubMed: 18812508] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 51142.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000806 Genomic DNA. Translation: ACB49395.1.
RefSeq	YP_001801461.1. NC_010546.1.
3D structure databases
ProteinModelPortal	B1WYG5.
ModBase	Search...
Protein-protein interaction databases
STRING	B1WYG5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6167307.
GenomeReviews	Gene locus cce_0043 in contig CP000806_GR.
KEGG	cyt:cce_0043.
PATRIC	21538560. VBICyaSp130209_0132.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG671955.
OMA	HITIIHI.
ProtClustDB	CLSK2301919.
Family and domain databases
HAMAP	MF_00067. GmhA. [Tree]
InterPro	IPR004515. Phosphoheptose_Isoase_subgr. IPR020620. Phosphoheptose_isomerase. IPR001347. SIS. [Graphical view]
KO	K03271.
TIGRFAMs	TIGR00441. GmhA. 1 hit.
PROSITE	PS51464. SIS. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GMHA_CYAA5

Accession

Primary (citable) accession number: B1WYG5

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	May 20, 2008
Last modified:	January 25, 2012
This is version 28 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program