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B1WVA3

- SYI_CYAA5

UniProt

B1WVA3 - SYI_CYAA5

Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Cyanothece sp. (strain ATCC 51142)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 49 (01 Oct 2014)
      Sequence version 1 (20 May 2008)
      Previous versions | rss
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    Functioni

    Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

    Catalytic activityi

    ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

    Cofactori

    Binds 1 zinc ion per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei569 – 5691Aminoacyl-adenylateUniRule annotation
    Binding sitei613 – 6131ATPUniRule annotation
    Metal bindingi928 – 9281ZincUniRule annotation
    Metal bindingi931 – 9311ZincUniRule annotation
    Metal bindingi948 – 9481ZincUniRule annotation
    Metal bindingi951 – 9511ZincUniRule annotation

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-HAMAP
    3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
    4. zinc ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Enzyme and pathway databases

    BioCyciCSP43989:GKC8-4600-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
    Alternative name(s):
    Isoleucyl-tRNA synthetaseUniRule annotation
    Short name:
    IleRSUniRule annotation
    Gene namesi
    Name:ileSUniRule annotation
    Ordered Locus Names:cce_4545
    OrganismiCyanothece sp. (strain ATCC 51142)
    Taxonomic identifieri43989 [NCBI]
    Taxonomic lineageiBacteriaCyanobacteriaOscillatoriophycideaeChroococcalesCyanothece
    ProteomesiUP000001203: Chromosome circular

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 959959Isoleucine--tRNA ligasePRO_1000189145Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi43989.cce_4545.

    Structurei

    3D structure databases

    ProteinModelPortaliB1WVA3.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi60 – 7011"HIGH" regionAdd
    BLAST
    Motifi610 – 6145"KMSKS" region

    Domaini

    IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0060.
    HOGENOMiHOG000246402.
    KOiK01870.
    OMAiKPVHWCL.
    OrthoDBiEOG644ZM1.

    Family and domain databases

    Gene3Di1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPiMF_02002. Ile_tRNA_synth_type1.
    InterProiIPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
    [Graphical view]
    PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
    PfamiPF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF06827. zf-FPG_IleRS. 1 hit.
    [Graphical view]
    PRINTSiPR00984. TRNASYNTHILE.
    SUPFAMiSSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsiTIGR00392. ileS. 1 hit.
    PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    B1WVA3-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTDPKSYKDT VNLPKTNFSM RANAVKREPE LQKFWADNQI YEKLSQENPE    50
    EVFVLHDGPP YANGSLHMGH ALNKTLKDII NKYKLLRGYK VRYVPGWDCH 100
    GLPIELKVLQ SMKSKEREGL TPLKLRHKAR DFALKTQEEQ AKGFKRFGVW 150
    GDWENPYLTL TPEYEAAQIG VFGEMALKGY IYRGLKPVHW SPSSQTALAE 200
    AELEYPEGHT SRSIFAAFPI IKASKDTEEI LQPFLNKLGV AIWTTTPWTL 250
    PGNLAVALNP DLNYAVVEQN SDVCNYQYLI VAADLVERLS TTFETELTVK 300
    ATLPGKALEH TIYRHPLYDR ESEILIGGDY VTTESGTGLV HTAPGHGQED 350
    YMVGQRYGLG ILSPVDAKGN FTEEARQFAG LNVLKDANEV IINELKEKGS 400
    LLKEEAYQHK YPYDWRTKKP TIFRATEQWF ASVKGFRDAA LTAIKTVQWI 450
    PAQGENRITP MVSDRSDWCI SRQRSWGLPI PVFYDEETNE PLLTEETIKH 500
    IQTIFAEKGS DAWWEMSIEA LLPDQYKADA HKYRKGTDTM DVWFDSGSSW 550
    ASVAKQRPEL KYPADIYLEG SDQHRGWFQS SLLTSVAVNE IAPYKTVLTH 600
    GFVLDEKGHK MSKSLGNIVD PNVIINGGKN QKQEPPYGAD VLRLWVSSVD 650
    YSSDVPIGKT ILKQLSDIYR KIRNTARFLL GNLHDFDPKK DTVSYEELPE 700
    LDRYMLHRIT EVFTEVTDAF ETYQFFRFFQ TVQNFCVVDL SNFYLDIAKD 750
    RLYISHPESI RRRSCQTVLA IAIENLAKAI APVLCHMAED IWQFLPYETP 800
    YKSVFTAGWV KTSKQWENSE LSASWAKIRG IRNEVNNALE LARKEKAIGS 850
    SLDAKVLLYV PEQNLRQQLE KFNPADSLTG NHVDELRYFV LASQVELVDS 900
    LDSIKNADYH SESDLVSVGV IKAEGEKCDR CWNYSTKVGE FKDDPTICER 950
    CNAALVGDF 959
    Length:959
    Mass (Da):109,206
    Last modified:May 20, 2008 - v1
    Checksum:iE8822B12906F2AA0
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000806 Genomic DNA. Translation: ACB53893.1.
    RefSeqiYP_001805959.1. NC_010546.1.

    Genome annotation databases

    EnsemblBacteriaiACB53893; ACB53893; cce_4545.
    GeneIDi6171623.
    KEGGicyt:cce_4545.
    PATRICi21547430. VBICyaSp130209_4519.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000806 Genomic DNA. Translation: ACB53893.1 .
    RefSeqi YP_001805959.1. NC_010546.1.

    3D structure databases

    ProteinModelPortali B1WVA3.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 43989.cce_4545.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ACB53893 ; ACB53893 ; cce_4545 .
    GeneIDi 6171623.
    KEGGi cyt:cce_4545.
    PATRICi 21547430. VBICyaSp130209_4519.

    Phylogenomic databases

    eggNOGi COG0060.
    HOGENOMi HOG000246402.
    KOi K01870.
    OMAi KPVHWCL.
    OrthoDBi EOG644ZM1.

    Enzyme and pathway databases

    BioCyci CSP43989:GKC8-4600-MONOMER.

    Family and domain databases

    Gene3Di 1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPi MF_02002. Ile_tRNA_synth_type1.
    InterProi IPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
    [Graphical view ]
    PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
    Pfami PF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF06827. zf-FPG_IleRS. 1 hit.
    [Graphical view ]
    PRINTSi PR00984. TRNASYNTHILE.
    SUPFAMi SSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsi TIGR00392. ileS. 1 hit.
    PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 51142.

    Entry informationi

    Entry nameiSYI_CYAA5
    AccessioniPrimary (citable) accession number: B1WVA3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 28, 2009
    Last sequence update: May 20, 2008
    Last modified: October 1, 2014
    This is version 49 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3