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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Cyanothece sp. (strain ATCC 51142)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei569 – 5691Aminoacyl-adenylateUniRule annotation
Binding sitei613 – 6131ATPUniRule annotation
Metal bindingi928 – 9281ZincUniRule annotation
Metal bindingi931 – 9311ZincUniRule annotation
Metal bindingi948 – 9481ZincUniRule annotation
Metal bindingi951 – 9511ZincUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciCSP43989:GKC8-4600-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:cce_4545
OrganismiCyanothece sp. (strain ATCC 51142)
Taxonomic identifieri43989 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaOscillatoriophycideaeChroococcalesCyanothece
ProteomesiUP000001203 Componenti: Chromosome circular

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 959959Isoleucine--tRNA ligasePRO_1000189145Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi43989.cce_4545.

Structurei

3D structure databases

ProteinModelPortaliB1WVA3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi60 – 7011"HIGH" regionAdd
BLAST
Motifi610 – 6145"KMSKS" region

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiVLGDWDN.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B1WVA3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTDPKSYKDT VNLPKTNFSM RANAVKREPE LQKFWADNQI YEKLSQENPE
60 70 80 90 100
EVFVLHDGPP YANGSLHMGH ALNKTLKDII NKYKLLRGYK VRYVPGWDCH
110 120 130 140 150
GLPIELKVLQ SMKSKEREGL TPLKLRHKAR DFALKTQEEQ AKGFKRFGVW
160 170 180 190 200
GDWENPYLTL TPEYEAAQIG VFGEMALKGY IYRGLKPVHW SPSSQTALAE
210 220 230 240 250
AELEYPEGHT SRSIFAAFPI IKASKDTEEI LQPFLNKLGV AIWTTTPWTL
260 270 280 290 300
PGNLAVALNP DLNYAVVEQN SDVCNYQYLI VAADLVERLS TTFETELTVK
310 320 330 340 350
ATLPGKALEH TIYRHPLYDR ESEILIGGDY VTTESGTGLV HTAPGHGQED
360 370 380 390 400
YMVGQRYGLG ILSPVDAKGN FTEEARQFAG LNVLKDANEV IINELKEKGS
410 420 430 440 450
LLKEEAYQHK YPYDWRTKKP TIFRATEQWF ASVKGFRDAA LTAIKTVQWI
460 470 480 490 500
PAQGENRITP MVSDRSDWCI SRQRSWGLPI PVFYDEETNE PLLTEETIKH
510 520 530 540 550
IQTIFAEKGS DAWWEMSIEA LLPDQYKADA HKYRKGTDTM DVWFDSGSSW
560 570 580 590 600
ASVAKQRPEL KYPADIYLEG SDQHRGWFQS SLLTSVAVNE IAPYKTVLTH
610 620 630 640 650
GFVLDEKGHK MSKSLGNIVD PNVIINGGKN QKQEPPYGAD VLRLWVSSVD
660 670 680 690 700
YSSDVPIGKT ILKQLSDIYR KIRNTARFLL GNLHDFDPKK DTVSYEELPE
710 720 730 740 750
LDRYMLHRIT EVFTEVTDAF ETYQFFRFFQ TVQNFCVVDL SNFYLDIAKD
760 770 780 790 800
RLYISHPESI RRRSCQTVLA IAIENLAKAI APVLCHMAED IWQFLPYETP
810 820 830 840 850
YKSVFTAGWV KTSKQWENSE LSASWAKIRG IRNEVNNALE LARKEKAIGS
860 870 880 890 900
SLDAKVLLYV PEQNLRQQLE KFNPADSLTG NHVDELRYFV LASQVELVDS
910 920 930 940 950
LDSIKNADYH SESDLVSVGV IKAEGEKCDR CWNYSTKVGE FKDDPTICER

CNAALVGDF
Length:959
Mass (Da):109,206
Last modified:May 20, 2008 - v1
Checksum:iE8822B12906F2AA0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000806 Genomic DNA. Translation: ACB53893.1.
RefSeqiWP_012362482.1. NC_010546.1.
YP_001805959.1. NC_010546.1.

Genome annotation databases

EnsemblBacteriaiACB53893; ACB53893; cce_4545.
KEGGicyt:cce_4545.
PATRICi21547430. VBICyaSp130209_4519.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000806 Genomic DNA. Translation: ACB53893.1.
RefSeqiWP_012362482.1. NC_010546.1.
YP_001805959.1. NC_010546.1.

3D structure databases

ProteinModelPortaliB1WVA3.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi43989.cce_4545.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACB53893; ACB53893; cce_4545.
KEGGicyt:cce_4545.
PATRICi21547430. VBICyaSp130209_4519.

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiVLGDWDN.
OrthoDBiEOG644ZM1.

Enzyme and pathway databases

BioCyciCSP43989:GKC8-4600-MONOMER.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 51142.

Entry informationi

Entry nameiSYI_CYAA5
AccessioniPrimary (citable) accession number: B1WVA3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: May 20, 2008
Last modified: May 27, 2015
This is version 54 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.