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B1WTI3

- BIOB_CYAA5

UniProt

B1WTI3 - BIOB_CYAA5

Protein

Biotin synthase

Gene

bioB

Organism
Cyanothece sp. (strain ATCC 51142)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 47 (01 Oct 2014)
      Sequence version 2 (28 Jul 2009)
      Previous versions | rss
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    Functioni

    Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism.UniRule annotation

    Catalytic activityi

    Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

    Cofactori

    Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation
    Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi101 – 1011Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi105 – 1051Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi108 – 1081Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi146 – 1461Iron-sulfur 2 (2Fe-2S)UniRule annotation
    Metal bindingi183 – 1831Iron-sulfur 2 (2Fe-2S)UniRule annotation
    Metal bindingi243 – 2431Iron-sulfur 2 (2Fe-2S)UniRule annotation
    Metal bindingi313 – 3131Iron-sulfur 2 (2Fe-2S)UniRule annotation

    GO - Molecular functioni

    1. 2 iron, 2 sulfur cluster binding Source: UniProtKB-KW
    2. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
    3. biotin synthase activity Source: UniProtKB-HAMAP
    4. iron ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. biotin biosynthetic process Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Transferase

    Keywords - Biological processi

    Biotin biosynthesis

    Keywords - Ligandi

    2Fe-2S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

    Enzyme and pathway databases

    BioCyciCSP43989:GKC8-4405-MONOMER.
    UniPathwayiUPA00078; UER00162.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Biotin synthaseUniRule annotation (EC:2.8.1.6UniRule annotation)
    Gene namesi
    Name:bioBUniRule annotation
    Ordered Locus Names:cce_4350
    OrganismiCyanothece sp. (strain ATCC 51142)
    Taxonomic identifieri43989 [NCBI]
    Taxonomic lineageiBacteriaCyanobacteriaOscillatoriophycideaeChroococcalesCyanothece
    ProteomesiUP000001203: Chromosome circular

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 382382Biotin synthasePRO_0000381337Add
    BLAST

    Interactioni

    Subunit structurei

    Homodimer.UniRule annotation

    Protein-protein interaction databases

    STRINGi43989.cce_4350.

    Structurei

    3D structure databases

    ProteinModelPortaliB1WTI3.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the radical SAM superfamily. Biotin synthase family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0502.
    HOGENOMiHOG000239958.
    KOiK01012.
    OrthoDBiEOG622PMP.

    Family and domain databases

    Gene3Di3.20.20.70. 1 hit.
    HAMAPiMF_01694. BioB.
    InterProiIPR013785. Aldolase_TIM.
    IPR010722. BATS_dom.
    IPR002684. Biotin_synth/BioAB.
    IPR024177. Biotin_synthase.
    IPR006638. Elp3/MiaB/NifB.
    IPR007197. rSAM.
    [Graphical view]
    PfamiPF06968. BATS. 1 hit.
    PF04055. Radical_SAM. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001619. Biotin_synth. 1 hit.
    SMARTiSM00876. BATS. 1 hit.
    SM00729. Elp3. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR00433. bioB. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    B1WTI3-1 [UniParc]FASTAAdd to Basket

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    MIQFIRPKTA SGKTIVVQLS ASPPQTPPPT DTNALKNWLE ILSQRIINGD    50
    RLTKTEALTL TQIDGQDNIL LLCEAADRIR QACCGNVVDL CSIINVKSGN 100
    CSENCQFCSQ SVHHQGENSP IYGLKSSEEI LAQAKAAEVA GAKRFCLVSQ 150
    GRGPKYNSPQ SGEFEKILET VRQITTETNI KPCCALGEVT LEQAEALKEA 200
    GVTRYNHNLE ASENYYQSIV STHSWGDRVE TVKNLKKAGI QACTGGIMGM 250
    GETWEDRVDL AISLRELEVD SVPINLLNPR EGTPLGHLPK LDVFDALKAI 300
    AIFRFLLPEQ ILRYAGGREA VMGELQSLGL KSGINAMLIG HYLTTLGQPP 350
    ENDHAMLESL GLQGGEAPIP GEYKKKTQKK AH 382
    Length:382
    Mass (Da):41,661
    Last modified:July 28, 2009 - v2
    Checksum:i1E1DCA94739F0817
    GO

    Sequence cautioni

    The sequence ACB53698.1 differs from that shown. Reason: Erroneous initiation.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000806 Genomic DNA. Translation: ACB53698.1. Different initiation.
    RefSeqiWP_012362429.1. NC_010546.1.
    YP_001805764.1. NC_010546.1.

    Genome annotation databases

    EnsemblBacteriaiACB53698; ACB53698; cce_4350.
    GeneIDi6171428.
    KEGGicyt:cce_4350.
    PATRICi21547038. VBICyaSp130209_4323.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000806 Genomic DNA. Translation: ACB53698.1 . Different initiation.
    RefSeqi WP_012362429.1. NC_010546.1.
    YP_001805764.1. NC_010546.1.

    3D structure databases

    ProteinModelPortali B1WTI3.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 43989.cce_4350.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ACB53698 ; ACB53698 ; cce_4350 .
    GeneIDi 6171428.
    KEGGi cyt:cce_4350.
    PATRICi 21547038. VBICyaSp130209_4323.

    Phylogenomic databases

    eggNOGi COG0502.
    HOGENOMi HOG000239958.
    KOi K01012.
    OrthoDBi EOG622PMP.

    Enzyme and pathway databases

    UniPathwayi UPA00078 ; UER00162 .
    BioCyci CSP43989:GKC8-4405-MONOMER.

    Family and domain databases

    Gene3Di 3.20.20.70. 1 hit.
    HAMAPi MF_01694. BioB.
    InterProi IPR013785. Aldolase_TIM.
    IPR010722. BATS_dom.
    IPR002684. Biotin_synth/BioAB.
    IPR024177. Biotin_synthase.
    IPR006638. Elp3/MiaB/NifB.
    IPR007197. rSAM.
    [Graphical view ]
    Pfami PF06968. BATS. 1 hit.
    PF04055. Radical_SAM. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001619. Biotin_synth. 1 hit.
    SMARTi SM00876. BATS. 1 hit.
    SM00729. Elp3. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR00433. bioB. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 51142.

    Entry informationi

    Entry nameiBIOB_CYAA5
    AccessioniPrimary (citable) accession number: B1WTI3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 28, 2009
    Last sequence update: July 28, 2009
    Last modified: October 1, 2014
    This is version 47 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3