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Protein

Biotin synthase

Gene

bioB

Organism
Cyanothece sp. (strain ATCC 51142)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism.UniRule annotation

Catalytic activityi

Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine + 2 reduced [2Fe-2S] ferredoxin = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine + 2 oxidized [2Fe-2S] ferredoxin.UniRule annotation

Cofactori

Protein has several cofactor binding sites:
  • [4Fe-4S] clusterUniRule annotationNote: Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation
  • [2Fe-2S] clusterUniRule annotationNote: Binds 1 [2Fe-2S] cluster. The cluster is coordinated with 3 cysteines and 1 arginine.UniRule annotation

Pathwayi: biotin biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes biotin from 7,8-diaminononanoate.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. ATP-dependent dethiobiotin synthetase BioD (bioD)
  2. Biotin synthase (bioB)
This subpathway is part of the pathway biotin biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes biotin from 7,8-diaminononanoate, the pathway biotin biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi101Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation1
Metal bindingi105Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation1
Metal bindingi108Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation1
Metal bindingi146Iron-sulfur 2 (2Fe-2S)UniRule annotation1
Metal bindingi183Iron-sulfur 2 (2Fe-2S)UniRule annotation1
Metal bindingi243Iron-sulfur 2 (2Fe-2S)UniRule annotation1
Metal bindingi313Iron-sulfur 2 (2Fe-2S)UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferase
Biological processBiotin biosynthesis
Ligand2Fe-2S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

UniPathwayiUPA00078; UER00162

Names & Taxonomyi

Protein namesi
Recommended name:
Biotin synthaseUniRule annotation (EC:2.8.1.6UniRule annotation)
Gene namesi
Name:bioBUniRule annotation
Ordered Locus Names:cce_4350
OrganismiCyanothece sp. (strain ATCC 51142)
Taxonomic identifieri43989 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaOscillatoriophycideaeOscillatorialesCyanothecaceaeCyanothece
Proteomesi
  • UP000001203 Componenti: Chromosome circular

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003813371 – 382Biotin synthaseAdd BLAST382

Proteomic databases

PRIDEiB1WTI3

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi43989.cce_4350

Structurei

3D structure databases

ProteinModelPortaliB1WTI3
SMRiB1WTI3
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the radical SAM superfamily. Biotin synthase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4107QSQ Bacteria
COG0502 LUCA
HOGENOMiHOG000239958
KOiK01012
OrthoDBiPOG091H01DF

Family and domain databases

Gene3Di3.20.20.70, 1 hit
HAMAPiMF_01694 BioB, 1 hit
InterProiView protein in InterPro
IPR013785 Aldolase_TIM
IPR010722 BATS_dom
IPR034416 BATS_domain_containing
IPR002684 Biotin_synth/BioAB
IPR024177 Biotin_synthase
IPR006638 Elp3/MiaB/NifB
IPR007197 rSAM
PANTHERiPTHR22976 PTHR22976, 1 hit
PfamiView protein in Pfam
PF06968 BATS, 1 hit
PF04055 Radical_SAM, 1 hit
PIRSFiPIRSF001619 Biotin_synth, 1 hit
SFLDiSFLDG01060 BATS_domain_containing, 1 hit
SFLDG01278 biotin_synthase_like, 1 hit
SFLDS00029 Radical_SAM, 1 hit
SMARTiView protein in SMART
SM00876 BATS, 1 hit
SM00729 Elp3, 1 hit
TIGRFAMsiTIGR00433 bioB, 1 hit

Sequencei

Sequence statusi: Complete.

B1WTI3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIQFIRPKTA SGKTIVVQLS ASPPQTPPPT DTNALKNWLE ILSQRIINGD
60 70 80 90 100
RLTKTEALTL TQIDGQDNIL LLCEAADRIR QACCGNVVDL CSIINVKSGN
110 120 130 140 150
CSENCQFCSQ SVHHQGENSP IYGLKSSEEI LAQAKAAEVA GAKRFCLVSQ
160 170 180 190 200
GRGPKYNSPQ SGEFEKILET VRQITTETNI KPCCALGEVT LEQAEALKEA
210 220 230 240 250
GVTRYNHNLE ASENYYQSIV STHSWGDRVE TVKNLKKAGI QACTGGIMGM
260 270 280 290 300
GETWEDRVDL AISLRELEVD SVPINLLNPR EGTPLGHLPK LDVFDALKAI
310 320 330 340 350
AIFRFLLPEQ ILRYAGGREA VMGELQSLGL KSGINAMLIG HYLTTLGQPP
360 370 380
ENDHAMLESL GLQGGEAPIP GEYKKKTQKK AH
Length:382
Mass (Da):41,661
Last modified:July 28, 2009 - v2
Checksum:i1E1DCA94739F0817
GO

Sequence cautioni

The sequence ACB53698 differs from that shown. Reason: Erroneous initiation.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000806 Genomic DNA Translation: ACB53698.1 Different initiation.

Genome annotation databases

EnsemblBacteriaiACB53698; ACB53698; cce_4350
KEGGicyt:cce_4350

Similar proteinsi

Entry informationi

Entry nameiBIOB_CYAA5
AccessioniPrimary (citable) accession number: B1WTI3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: July 28, 2009
Last modified: May 23, 2018
This is version 61 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
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