Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

B1WTI3

- BIOB_CYAA5

UniProt

B1WTI3 - BIOB_CYAA5

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Biotin synthase

Gene

bioB

Organism
Cyanothece sp. (strain ATCC 51142)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism.UniRule annotation

Catalytic activityi

Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

Cofactori

Protein has several cofactor binding sites:
  • [4Fe-4S] clusterUniRule annotationNote: Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation
  • [2Fe-2S] clusterUniRule annotationNote: Binds 1 [2Fe-2S] cluster. The cluster is coordinated with 3 cysteines and 1 arginine.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi101 – 1011Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation
Metal bindingi105 – 1051Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation
Metal bindingi108 – 1081Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation
Metal bindingi146 – 1461Iron-sulfur 2 (2Fe-2S)UniRule annotation
Metal bindingi183 – 1831Iron-sulfur 2 (2Fe-2S)UniRule annotation
Metal bindingi243 – 2431Iron-sulfur 2 (2Fe-2S)UniRule annotation
Metal bindingi313 – 3131Iron-sulfur 2 (2Fe-2S)UniRule annotation

GO - Molecular functioni

  1. 2 iron, 2 sulfur cluster binding Source: UniProtKB-KW
  2. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
  3. biotin synthase activity Source: UniProtKB-HAMAP
  4. iron ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. biotin biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Biotin biosynthesis

Keywords - Ligandi

2Fe-2S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciCSP43989:GKC8-4405-MONOMER.
UniPathwayiUPA00078; UER00162.

Names & Taxonomyi

Protein namesi
Recommended name:
Biotin synthaseUniRule annotation (EC:2.8.1.6UniRule annotation)
Gene namesi
Name:bioBUniRule annotation
Ordered Locus Names:cce_4350
OrganismiCyanothece sp. (strain ATCC 51142)
Taxonomic identifieri43989 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaOscillatoriophycideaeChroococcalesCyanothece
ProteomesiUP000001203: Chromosome circular

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 382382Biotin synthasePRO_0000381337Add
BLAST

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi43989.cce_4350.

Structurei

3D structure databases

ProteinModelPortaliB1WTI3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the radical SAM superfamily. Biotin synthase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0502.
HOGENOMiHOG000239958.
KOiK01012.
OrthoDBiEOG622PMP.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_01694. BioB.
InterProiIPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view]
PfamiPF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFiPIRSF001619. Biotin_synth. 1 hit.
SMARTiSM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00433. bioB. 1 hit.

Sequencei

Sequence statusi: Complete.

B1WTI3-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MIQFIRPKTA SGKTIVVQLS ASPPQTPPPT DTNALKNWLE ILSQRIINGD
60 70 80 90 100
RLTKTEALTL TQIDGQDNIL LLCEAADRIR QACCGNVVDL CSIINVKSGN
110 120 130 140 150
CSENCQFCSQ SVHHQGENSP IYGLKSSEEI LAQAKAAEVA GAKRFCLVSQ
160 170 180 190 200
GRGPKYNSPQ SGEFEKILET VRQITTETNI KPCCALGEVT LEQAEALKEA
210 220 230 240 250
GVTRYNHNLE ASENYYQSIV STHSWGDRVE TVKNLKKAGI QACTGGIMGM
260 270 280 290 300
GETWEDRVDL AISLRELEVD SVPINLLNPR EGTPLGHLPK LDVFDALKAI
310 320 330 340 350
AIFRFLLPEQ ILRYAGGREA VMGELQSLGL KSGINAMLIG HYLTTLGQPP
360 370 380
ENDHAMLESL GLQGGEAPIP GEYKKKTQKK AH
Length:382
Mass (Da):41,661
Last modified:July 28, 2009 - v2
Checksum:i1E1DCA94739F0817
GO

Sequence cautioni

The sequence ACB53698.1 differs from that shown. Reason: Erroneous initiation. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000806 Genomic DNA. Translation: ACB53698.1. Different initiation.
RefSeqiWP_012362429.1. NC_010546.1.
YP_001805764.1. NC_010546.1.

Genome annotation databases

EnsemblBacteriaiACB53698; ACB53698; cce_4350.
GeneIDi6171428.
KEGGicyt:cce_4350.
PATRICi21547038. VBICyaSp130209_4323.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000806 Genomic DNA. Translation: ACB53698.1 . Different initiation.
RefSeqi WP_012362429.1. NC_010546.1.
YP_001805764.1. NC_010546.1.

3D structure databases

ProteinModelPortali B1WTI3.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 43989.cce_4350.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ACB53698 ; ACB53698 ; cce_4350 .
GeneIDi 6171428.
KEGGi cyt:cce_4350.
PATRICi 21547038. VBICyaSp130209_4323.

Phylogenomic databases

eggNOGi COG0502.
HOGENOMi HOG000239958.
KOi K01012.
OrthoDBi EOG622PMP.

Enzyme and pathway databases

UniPathwayi UPA00078 ; UER00162 .
BioCyci CSP43989:GKC8-4405-MONOMER.

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
HAMAPi MF_01694. BioB.
InterProi IPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view ]
Pfami PF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view ]
PIRSFi PIRSF001619. Biotin_synth. 1 hit.
SMARTi SM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00433. bioB. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 51142.

Entry informationi

Entry nameiBIOB_CYAA5
AccessioniPrimary (citable) accession number: B1WTI3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: July 28, 2009
Last modified: November 26, 2014
This is version 48 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3