B1WTI3 (BIOB_CYAA5) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Biotin synthase
EC=2.8.1.6

Gene names

Name:	bioB
Ordered Locus Names:	cce_4350

Organism

Cyanothece sp. (strain ATCC 51142) [Complete proteome] [HAMAP]

Taxonomic identifier

43989 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Cyanobacteria › Oscillatoriophycideae › Chroococcales › Cyanothece

Protein attributes

Sequence length	382 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism By similarity. HAMAP-Rule MF_01694
Catalytic activity	Dethiobiotin + sulfur + 2 S-adenosyl-L-methionine = biotin + 2 L-methionine + 2 5'-deoxyadenosine. HAMAP-Rule MF_01694
Cofactor	Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity. Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine By similarity.
Pathway	Cofactor biosynthesis; biotin biosynthesis; biotin from 7,8-diaminononanoate: step 2/2. HAMAP-Rule MF_01694
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the radical SAM superfamily. Biotin synthase family.
Sequence caution	The sequence ACB53698.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Biotin biosynthesis
Ligand	2Fe-2S 4Fe-4S Iron Iron-sulfur Metal-binding S-adenosyl-L-methionine
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	biotin biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular_function	2 iron, 2 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW 4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW biotin synthase activity Inferred from electronic annotation. Source: HAMAP iron ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 382	382	Biotin synthase HAMAP-Rule MF_01694		PRO_0000381337
Sites
	Metal binding	101	1	Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
	Metal binding	105	1	Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
	Metal binding	108	1	Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
	Metal binding	146	1	Iron-sulfur 2 (2Fe-2S) By similarity
	Metal binding	183	1	Iron-sulfur 2 (2Fe-2S) By similarity
	Metal binding	243	1	Iron-sulfur 2 (2Fe-2S) By similarity
	Metal binding	313	1	Iron-sulfur 2 (2Fe-2S) By similarity

Sequences

	Sequence		Length	Mass (Da)
	B1WTI3 [UniParc]. Last modified July 28, 2009. Version 2. Checksum: 1E1DCA94739F0817	FASTA	382	41,661
	10 20 30 40 50 60 MIQFIRPKTA SGKTIVVQLS ASPPQTPPPT DTNALKNWLE ILSQRIINGD RLTKTEALTL 70 80 90 100 110 120 TQIDGQDNIL LLCEAADRIR QACCGNVVDL CSIINVKSGN CSENCQFCSQ SVHHQGENSP 130 140 150 160 170 180 IYGLKSSEEI LAQAKAAEVA GAKRFCLVSQ GRGPKYNSPQ SGEFEKILET VRQITTETNI 190 200 210 220 230 240 KPCCALGEVT LEQAEALKEA GVTRYNHNLE ASENYYQSIV STHSWGDRVE TVKNLKKAGI 250 260 270 280 290 300 QACTGGIMGM GETWEDRVDL AISLRELEVD SVPINLLNPR EGTPLGHLPK LDVFDALKAI 310 320 330 340 350 360 AIFRFLLPEQ ILRYAGGREA VMGELQSLGL KSGINAMLIG HYLTTLGQPP ENDHAMLESL 370 380 GLQGGEAPIP GEYKKKTQKK AH

References

[1]

"The genome of Cyanothece 51142, a unicellular diazotrophic cyanobacterium important in the marine nitrogen cycle." Welsh E.A., Liberton M., Stoeckel J., Loh T., Elvitigala T., Wang C., Wollam A., Fulton R.S., Clifton S.W., Jacobs J.M., Aurora R., Ghosh B.K., Sherman L.A., Smith R.D., Wilson R.K., Pakrasi H.B. Proc. Natl. Acad. Sci. U.S.A. 105:15094-15099(2008) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 51142.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000806 Genomic DNA. Translation: ACB53698.1. Different initiation.
RefSeq	YP_001805764.1. NC_010546.1.
3D structure databases
ProteinModelPortal	B1WTI3.
ModBase	Search...
Protein-protein interaction databases
STRING	43989.cce_4350.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ACB53698; ACB53698; cce_4350.
GeneID	6171428.
KEGG	cyt:cce_4350.
PATRIC	21547038. VBICyaSp130209_4323.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0502.
HOGENOM	HOG000239958.
KO	K01012.
ProtClustDB	CLSK892897.
Enzyme and pathway databases
UniPathway	UPA00078; UER00162.
Family and domain databases
Gene3D	3.20.20.70. 1 hit.
HAMAP	MF_01694. BioB.
InterPro	IPR013785. Aldolase_TIM. IPR010722. Biotin/thiamin_synth-assoc. IPR002684. Biotin_synth/BioAB. IPR024177. Biotin_synthase. IPR006638. Elp3/MiaB/NifB. IPR007197. rSAM. [Graphical view]
Pfam	PF06968. BATS. 1 hit. PF04055. Radical_SAM. 1 hit. [Graphical view]
PIRSF	PIRSF001619. Biotin_synth. 1 hit.
SMART	SM00876. BATS. 1 hit. SM00729. Elp3. 1 hit. [Graphical view]
TIGRFAMs	TIGR00433. bioB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

BIOB_CYAA5

Accession

Primary (citable) accession number: B1WTI3

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 28, 2009
Last sequence update:	July 28, 2009
Last modified:	May 1, 2013
This is version 38 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program