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B1WTI3 (BIOB_CYAA5) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 44. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Biotin synthase

EC=2.8.1.6
Gene names
Name:bioB
Ordered Locus Names:cce_4350
OrganismCyanothece sp. (strain ATCC 51142) [Complete proteome] [HAMAP]
Taxonomic identifier43989 [NCBI]
Taxonomic lineageBacteriaCyanobacteriaOscillatoriophycideaeChroococcalesCyanothece

Protein attributes

Sequence length382 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism By similarity. HAMAP-Rule MF_01694

Catalytic activity

Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine. HAMAP-Rule MF_01694

Cofactor

Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.

Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine By similarity.

Pathway

Cofactor biosynthesis; biotin biosynthesis; biotin from 7,8-diaminononanoate: step 2/2. HAMAP-Rule MF_01694

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_01694

Sequence similarities

Belongs to the radical SAM superfamily. Biotin synthase family.

Sequence caution

The sequence ACB53698.1 differs from that shown. Reason: Erroneous initiation.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 382382Biotin synthase HAMAP-Rule MF_01694
PRO_0000381337

Sites

Metal binding1011Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding1051Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding1081Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding1461Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding1831Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding2431Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding3131Iron-sulfur 2 (2Fe-2S) By similarity

Sequences

Sequence LengthMass (Da)Tools
B1WTI3 [UniParc].

Last modified July 28, 2009. Version 2.
Checksum: 1E1DCA94739F0817

FASTA38241,661
        10         20         30         40         50         60 
MIQFIRPKTA SGKTIVVQLS ASPPQTPPPT DTNALKNWLE ILSQRIINGD RLTKTEALTL 

        70         80         90        100        110        120 
TQIDGQDNIL LLCEAADRIR QACCGNVVDL CSIINVKSGN CSENCQFCSQ SVHHQGENSP 

       130        140        150        160        170        180 
IYGLKSSEEI LAQAKAAEVA GAKRFCLVSQ GRGPKYNSPQ SGEFEKILET VRQITTETNI 

       190        200        210        220        230        240 
KPCCALGEVT LEQAEALKEA GVTRYNHNLE ASENYYQSIV STHSWGDRVE TVKNLKKAGI 

       250        260        270        280        290        300 
QACTGGIMGM GETWEDRVDL AISLRELEVD SVPINLLNPR EGTPLGHLPK LDVFDALKAI 

       310        320        330        340        350        360 
AIFRFLLPEQ ILRYAGGREA VMGELQSLGL KSGINAMLIG HYLTTLGQPP ENDHAMLESL 

       370        380 
GLQGGEAPIP GEYKKKTQKK AH 

« Hide

References

[1]"The genome of Cyanothece 51142, a unicellular diazotrophic cyanobacterium important in the marine nitrogen cycle."
Welsh E.A., Liberton M., Stoeckel J., Loh T., Elvitigala T., Wang C., Wollam A., Fulton R.S., Clifton S.W., Jacobs J.M., Aurora R., Ghosh B.K., Sherman L.A., Smith R.D., Wilson R.K., Pakrasi H.B.
Proc. Natl. Acad. Sci. U.S.A. 105:15094-15099(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 51142.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000806 Genomic DNA. Translation: ACB53698.1. Different initiation.
RefSeqYP_001805764.1. NC_010546.1.

3D structure databases

ProteinModelPortalB1WTI3.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING43989.cce_4350.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACB53698; ACB53698; cce_4350.
GeneID6171428.
KEGGcyt:cce_4350.
PATRIC21547038. VBICyaSp130209_4323.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0502.
HOGENOMHOG000239958.
KOK01012.
OrthoDBEOG622PMP.
ProtClustDBCLSK892897.

Enzyme and pathway databases

BioCycCSP43989:GKC8-4405-MONOMER.
UniPathwayUPA00078; UER00162.

Family and domain databases

Gene3D3.20.20.70. 1 hit.
HAMAPMF_01694. BioB.
InterProIPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view]
PfamPF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFPIRSF001619. Biotin_synth. 1 hit.
SMARTSM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsTIGR00433. bioB. 1 hit.
ProtoNetSearch...

Entry information

Entry nameBIOB_CYAA5
AccessionPrimary (citable) accession number: B1WTI3
Entry history
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: July 28, 2009
Last modified: February 19, 2014
This is version 44 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways