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Protein

1,4-alpha-glucan branching enzyme GlgB

Gene

glgB

Organism
Cyanothece sp. (strain ATCC 51142)
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position.UniRule annotation

Catalytic activityi

Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain.UniRule annotationSAAS annotation

Pathwayi: glycogen biosynthesis

This protein is involved in the pathway glycogen biosynthesis, which is part of Glycan biosynthesis.UniRule annotationSAAS annotation
View all proteins of this organism that are known to be involved in the pathway glycogen biosynthesis and in Glycan biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei8GlucoseCombined sources1
Binding sitei150GlucoseCombined sources1
Binding sitei163GlucoseCombined sources1
Binding sitei175Glucose; via carbonyl oxygenCombined sources1
Binding sitei192GlucoseCombined sources1
Binding sitei204GlucoseCombined sources1
Binding sitei373GlucoseCombined sources1
Binding sitei399Glucose; via carbonyl oxygenCombined sources1
Active sitei434NucleophileUniRule annotation1
Binding sitei434GlucoseCombined sources1
Binding sitei476GlucoseCombined sources1
Active sitei487Proton donorUniRule annotation1
Binding sitei547GlucoseCombined sources1
Binding sitei687Glucose; via carbonyl oxygenCombined sources1
Binding sitei733GlucoseCombined sources1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosyltransferaseUniRule annotationSAAS annotation, Transferase
Biological processCarbohydrate metabolism, Glycogen biosynthesisUniRule annotationSAAS annotation, Glycogen metabolismUniRule annotation

Enzyme and pathway databases

UniPathwayiUPA00164.

Protein family/group databases

CAZyiCBM48. Carbohydrate-Binding Module Family 48.
GH13. Glycoside Hydrolase Family 13.

Names & Taxonomyi

Protein namesi
Recommended name:
1,4-alpha-glucan branching enzyme GlgBUniRule annotation (EC:2.4.1.18UniRule annotation)
Alternative name(s):
1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferaseUniRule annotation
Alpha-(1->4)-glucan branching enzymeUniRule annotation
Glycogen branching enzymeUniRule annotation
Short name:
BEUniRule annotation
Gene namesi
Name:glgBUniRule annotation
Synonyms:glgB1Imported
Ordered Locus Names:cce_2248Imported
OrganismiCyanothece sp. (strain ATCC 51142)Imported
Taxonomic identifieri43989 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaOscillatoriophycideaeOscillatorialesCyanothecaceaeCyanothece
Proteomesi
  • UP000001203 Componenti: Chromosome circular

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi43989.cce_2248.

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5GQUX-ray1.85A1-773[»]
5GQVX-ray3.00A1-773[»]
5GQWX-ray1.80A1-773[»]
5GQXX-ray2.30A1-773[»]
ProteinModelPortaliB1WPM8.
SMRiB1WPM8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini279 – 637AamyInterPro annotationAdd BLAST359

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni214 – 217Glucose bindingCombined sources4
Regioni282 – 284Glucose bindingCombined sources3
Regioni322 – 330Glucose bindingCombined sources9
Regioni407 – 410Glucose bindingCombined sources4
Regioni496 – 501Glucose bindingCombined sources6
Regioni505 – 506Glucose bindingCombined sources2
Regioni537 – 540Glucose bindingCombined sources4
Regioni554 – 555Glucose bindingCombined sources2
Regioni571 – 574Glucose bindingCombined sources4
Regioni655 – 657Glucose bindingCombined sources3
Regioni691 – 694Glucose bindingCombined sources4
Regioni705 – 708Glucose bindingCombined sources4
Regioni713 – 725Glucose bindingCombined sourcesAdd BLAST13

Sequence similaritiesi

Belongs to the glycosyl hydrolase 13 family. GlgB subfamily.UniRule annotationSAAS annotation

Phylogenomic databases

eggNOGiENOG4105C9C. Bacteria.
COG0296. LUCA.
HOGENOMiHOG000283037.
KOiK00700.
OMAiEVVHGKS.
OrthoDBiPOG091H08D1.

Family and domain databases

Gene3Di2.60.40.10. 2 hits.
HAMAPiMF_00685. GlgB. 1 hit.
InterProiView protein in InterPro
IPR006048. A-amylase/branching_C.
IPR006407. GlgB.
IPR006047. Glyco_hydro_13_cat_dom.
IPR004193. Glyco_hydro_13_N.
IPR017853. Glycoside_hydrolase_SF.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
PfamiView protein in Pfam
PF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
PF02922. CBM_48. 2 hits.
PIRSFiPIRSF000463. GlgB. 1 hit.
SMARTiView protein in SMART
SM00642. Aamy. 1 hit.
SUPFAMiSSF51445. SSF51445. 1 hit.
SSF81296. SSF81296. 2 hits.
TIGRFAMsiTIGR01515. branching_enzym. 1 hit.

Sequencei

Sequence statusi: Complete.

B1WPM8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTTTISADQV NQIIYNLHHD PFEILGCHLL EEGKNTKKWV VRAYLPKAEA
60 70 80 90 100
AWVIRPTERK EDPMNSVHHP NFFECIIETP ELNHYQLKVK EGEHEKVIYD
110 120 130 140 150
PYAFSSPYLT DEDIYLFSEG NHHRIYEKLG AHVGEINGVK GVYFAVWAPN
160 170 180 190 200
ARNVSVIGDF NNWDGREHQM RKRNYTIWEL FVPEIGSGTV YKYEIKNSEG
210 220 230 240 250
HIYEKSDPYG FYREVRPNTA SIVVDIDNIY QWHDEEWLEK RRNSDPLKQP
260 270 280 290 300
VSVYEVHLGS WLHGSSAEKM PLLNGEADPV IVSEWNPGAR FLSYYELAEK
310 320 330 340 350
LIPYVKDMGY THIELLPIAE HPFDGSWGYQ VTGFYSPTSR FGRPEDFMYF
360 370 380 390 400
VDKCHENGIG VILDWVPGHF PKDSHGLAYF DGTHLYEHAD PRIGEHKEWG
410 420 430 440 450
TLVFNYGRHE VRNFLVANVL FWFDKYHVDG IRVDAVASML YRNYLRKEGE
460 470 480 490 500
WIANEYGGDE HIEAVSFIRE VNTLLFEYFP GILSIAEEST EWEKVSRPVY
510 520 530 540 550
DGGLGFNLKW DMGWMHDMLD YFNIDPYFRQ YHQNNVTFSM LYYYNENFML
560 570 580 590 600
ALSHDEIVHG KSNMLGKMPG DEWQKYANVR ALFTYMYTHP GKKTMFMSME
610 620 630 640 650
FGQWSEWNVW GDLEWHLLQY EPHQQLKQFF TDLNALYQQE PALYTHDFEY
660 670 680 690 700
HGFEWIDCND NTHSVVSFLR RSDDPNDSLV VVCNFTPQPH SHYRIGVPEA
710 720 730 740 750
GYYVELFNSD AKQYGGSNMG NLGGKWADEW SFHNKPYSLD LCLPPLAVLI
760 770
LKLDPTKVPE GTTIKEIAAD EEE
Length:773
Mass (Da):90,410
Last modified:May 20, 2008 - v1
Checksum:iC91C69B3C9A4FFB5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000806 Genomic DNA. Translation: ACB51598.1.
RefSeqiWP_009546994.1. NC_010546.1.

Genome annotation databases

EnsemblBacteriaiACB51598; ACB51598; cce_2248.
KEGGicyt:cce_2248.

Similar proteinsi

Entry informationi

Entry nameiB1WPM8_CYAA5
AccessioniPrimary (citable) accession number: B1WPM8
Entry historyiIntegrated into UniProtKB/TrEMBL: May 20, 2008
Last sequence update: May 20, 2008
Last modified: September 27, 2017
This is version 81 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

3D-structureCombined sources, Complete proteome, Reference proteomeImported