Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Metallophosphoesterase 1

Gene

Mppe1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Metallophosphoesterase required for transport of GPI-anchor proteins from the endoplasmic reticulum to the Golgi. Acts in lipid remodeling steps of GPI-anchor maturation by mediating the removal of a side-chain ethanolamine-phosphate (EtNP) from the second Man (Man2) of the GPI intermediate, an essential step for efficient transport of GPI-anchor proteins (By similarity).By similarity

Cofactori

Mn2+By similarityNote: Binds 2 manganese ions per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi74 – 741Divalent metal cation 2By similarity
Metal bindingi116 – 1161Divalent metal cation 1By similarity
Metal bindingi116 – 1161Divalent metal cation 2By similarity
Metal bindingi154 – 1541Divalent metal cation 1By similarity
Metal bindingi247 – 2471Divalent metal cation 1By similarity
Metal bindingi301 – 3011Divalent metal cation 1By similarity
Metal bindingi303 – 3031Divalent metal cation 2By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

ER-Golgi transport, GPI-anchor biosynthesis, Transport

Keywords - Ligandi

Manganese, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Metallophosphoesterase 1 (EC:3.1.-.-)
Alternative name(s):
Post-GPI attachment to proteins factor 5
Gene namesi
Name:Mppe1
Synonyms:Pgap5
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 18

Organism-specific databases

RGDi1309184. Mppe1.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei27 – 4721HelicalSequence analysisAdd
BLAST
Transmembranei354 – 37421HelicalSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 394394Metallophosphoesterase 1PRO_0000355204Add
BLAST

Proteomic databases

PaxDbiB1WC86.
PRIDEiB1WC86.

PTM databases

PhosphoSiteiB1WC86.

Expressioni

Gene expression databases

GenevisibleiB1WC86. RN.

Interactioni

Subunit structurei

Interacts with GPI-anchor proteins. Interacts with TMED10 (By similarity).By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000025164.

Structurei

3D structure databases

ProteinModelPortaliB1WC86.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi17 – 204Poly-Arg

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3662. Eukaryota.
ENOG410ZM57. LUCA.
GeneTreeiENSGT00390000013236.
HOGENOMiHOG000006790.
HOVERGENiHBG101555.
InParanoidiB1WC86.
OMAiHYPLYRA.
OrthoDBiEOG722J8P.
PhylomeDBiB1WC86.
TreeFamiTF314437.

Family and domain databases

Gene3Di3.60.21.10. 1 hit.
InterProiIPR004843. Calcineurin-like_PHP_apaH.
IPR029052. Metallo-depent_PP-like.
IPR033308. PGAP5/Cdc1/Ted1.
[Graphical view]
PANTHERiPTHR13315. PTHR13315. 1 hit.
PfamiPF00149. Metallophos. 1 hit.
[Graphical view]
SUPFAMiSSF56300. SSF56300. 1 hit.

Sequencei

Sequence statusi: Complete.

B1WC86-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MALVRWGLRR QNFHLLRRRR VLLLKLTVVV ISVLLFCEYF IYYLVLFRCH
60 70 80 90 100
WPEVKMPARG GRQEPVLKAM FLADTHLLGE IRGHWLDKLR REWQMERAFQ
110 120 130 140 150
TALWLLQPEV VFILGDVFDE GKWSSAQAWA DDLHRFQRMF RHGSHVQLKV
160 170 180 190 200
VIGNHDIGFH YQMSKYRINR FEKVFGSERL FSLKGVNFVM VNSVAMEGDG
210 220 230 240 250
CTICSEAEAE LREISRKLNC SQEQVQGSSQ CDHEPRLPLS APVLLQHYPL
260 270 280 290 300
YRASDANCSG EDAAPPEERS VPFEEKYDVL SREASQKLLW WLRPRLILSG
310 320 330 340 350
HTHSACEVLH PGGAPEVSVP SFSWRNRNNP SFIMGSLTSR DYALSKCYLP
360 370 380 390
CEDTVLTTYC AAAAFLLVLI LAHFERLPSS FLFGWKLCRS HLRR
Length:394
Mass (Da):45,654
Last modified:May 20, 2008 - v1
Checksum:iC2C8F762424CECE6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CH473971 Genomic DNA. Translation: EDM14719.1.
BC162043 mRNA. Translation: AAI62043.1.
RefSeqiNP_001101905.1. NM_001108435.2.
XP_006254941.1. XM_006254879.2.
UniGeneiRn.64185.

Genome annotation databases

EnsembliENSRNOT00000025164; ENSRNOP00000025164; ENSRNOG00000018648.
GeneIDi361344.
KEGGirno:361344.
UCSCiRGD:1309184. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CH473971 Genomic DNA. Translation: EDM14719.1.
BC162043 mRNA. Translation: AAI62043.1.
RefSeqiNP_001101905.1. NM_001108435.2.
XP_006254941.1. XM_006254879.2.
UniGeneiRn.64185.

3D structure databases

ProteinModelPortaliB1WC86.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000025164.

PTM databases

PhosphoSiteiB1WC86.

Proteomic databases

PaxDbiB1WC86.
PRIDEiB1WC86.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000025164; ENSRNOP00000025164; ENSRNOG00000018648.
GeneIDi361344.
KEGGirno:361344.
UCSCiRGD:1309184. rat.

Organism-specific databases

CTDi65258.
RGDi1309184. Mppe1.

Phylogenomic databases

eggNOGiKOG3662. Eukaryota.
ENOG410ZM57. LUCA.
GeneTreeiENSGT00390000013236.
HOGENOMiHOG000006790.
HOVERGENiHBG101555.
InParanoidiB1WC86.
OMAiHYPLYRA.
OrthoDBiEOG722J8P.
PhylomeDBiB1WC86.
TreeFamiTF314437.

Miscellaneous databases

NextBioi675998.
PROiB1WC86.

Gene expression databases

GenevisibleiB1WC86. RN.

Family and domain databases

Gene3Di3.60.21.10. 1 hit.
InterProiIPR004843. Calcineurin-like_PHP_apaH.
IPR029052. Metallo-depent_PP-like.
IPR033308. PGAP5/Cdc1/Ted1.
[Graphical view]
PANTHERiPTHR13315. PTHR13315. 1 hit.
PfamiPF00149. Metallophos. 1 hit.
[Graphical view]
SUPFAMiSSF56300. SSF56300. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Brown Norway.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Heart.

Entry informationi

Entry nameiMPPE1_RAT
AccessioniPrimary (citable) accession number: B1WC86
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 25, 2008
Last sequence update: May 20, 2008
Last modified: May 11, 2016
This is version 59 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.