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B1WC68

- HDAC8_RAT

UniProt

B1WC68 - HDAC8_RAT

Protein

Histone deacetylase 8

Gene

Hdac8

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 45 (01 Oct 2014)
      Sequence version 1 (20 May 2008)
      Previous versions | rss
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    Functioni

    Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Also involved in the deacetylation of cohesin complex protein SMC3 regulating release of cohesin complexes from chromatin. May play a role in smooth muscle cell contractility By similarity.By similarity

    Catalytic activityi

    Hydrolysis of an N(6)-acetyl-lysine residue of a histone to yield a deacetylated histone.

    Cofactori

    Binds 1 divalent metal cation per subunit.

    Enzyme regulationi

    Its activity is inhibited by trichostatin A (TSA) and butyrate, 2 well known histone deacetylase inhibitors.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei101 – 1011SubstrateBy similarity
    Active sitei143 – 1431Proton acceptorBy similarity
    Binding sitei151 – 1511Substrate; via carbonyl oxygenBy similarity
    Metal bindingi178 – 1781Divalent metal cationBy similarity
    Metal bindingi180 – 1801Divalent metal cationBy similarity
    Metal bindingi267 – 2671Divalent metal cationBy similarity
    Binding sitei306 – 3061SubstrateBy similarity

    GO - Molecular functioni

    1. metal ion binding Source: UniProtKB-KW
    2. NAD-dependent histone deacetylase activity (H3-K14 specific) Source: UniProtKB-EC
    3. NAD-dependent histone deacetylase activity (H3-K18 specific) Source: UniProtKB-EC
    4. NAD-dependent histone deacetylase activity (H3-K9 specific) Source: UniProtKB-EC
    5. NAD-dependent histone deacetylase activity (H4-K16 specific) Source: UniProtKB-EC

    GO - Biological processi

    1. regulation of cohesin localization to chromatin Source: UniProtKB
    2. regulation of transcription, DNA-templated Source: UniProtKB-KW
    3. sister chromatid cohesion Source: UniProtKB
    4. transcription, DNA-templated Source: UniProtKB-KW

    Keywords - Molecular functioni

    Chromatin regulator, Hydrolase, Repressor

    Keywords - Biological processi

    Transcription, Transcription regulation

    Keywords - Ligandi

    Metal-binding

    Enzyme and pathway databases

    ReactomeiREACT_194781. Separation of Sister Chromatids.
    REACT_198611. Resolution of Sister Chromatid Cohesion.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Histone deacetylase 8 (EC:3.5.1.98)
    Short name:
    HD8
    Gene namesi
    Name:Hdac8
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Chromosome X

    Organism-specific databases

    RGDi1562895. Hdac8.

    Subcellular locationi

    Nucleus By similarity. Cytoplasm By similarity

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell
    2. nucleus Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm, Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 377377Histone deacetylase 8PRO_0000389508Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei39 – 391PhosphoserineBy similarity

    Post-translational modificationi

    Phosphorylated by PKA on serine 39. Phosphorylation reduces deacetylase activity observed preferentially on histones H3 and H4 By similarity.By similarity

    Keywords - PTMi

    Phosphoprotein

    Expressioni

    Gene expression databases

    GenevestigatoriB1WC68.

    Interactioni

    Subunit structurei

    Interacts with CBFA2T3. Interacts with phosphorylated SMG5/EST1B; this interaction protects SMG5 from ubiquitin-mediated degradation. Associates with alpha-SMA (smooth muscle alpha-actin) By similarity.By similarity

    Protein-protein interaction databases

    STRINGi10116.ENSRNOP00000004224.

    Structurei

    3D structure databases

    ProteinModelPortaliB1WC68.
    SMRiB1WC68. Positions 14-377.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni14 – 324311Histone deacetylaseBy similarityAdd
    BLAST

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG0123.
    GeneTreeiENSGT00530000062889.
    HOGENOMiHOG000225180.
    HOVERGENiHBG057112.
    KOiK11405.
    OMAiCLIDGKC.
    OrthoDBiEOG722J95.
    PhylomeDBiB1WC68.
    TreeFamiTF106175.

    Family and domain databases

    Gene3Di3.40.800.20. 1 hit.
    InterProiIPR000286. His_deacetylse.
    IPR003084. His_deacetylse_1.
    IPR023801. His_deacetylse_dom.
    [Graphical view]
    PANTHERiPTHR10625. PTHR10625. 1 hit.
    PfamiPF00850. Hist_deacetyl. 1 hit.
    [Graphical view]
    PIRSFiPIRSF037913. His_deacetylse_1. 1 hit.
    PRINTSiPR01270. HDASUPER.
    PR01271. HISDACETLASE.

    Sequencei

    Sequence statusi: Complete.

    B1WC68-1 [UniParc]FASTAAdd to Basket

    « Hide

    MEIPEEPANS GHSLPPVYIY SPEYVSICDS LVKVPKRASM VHSLIEAYAL    50
    HKQMRIVKPK VASMEEMATF HTDAYLQHLQ KVSQEGDEDH PDSIEYGLGY 100
    DCPATEGIFD YAAAIGGGTI TAAQCLIDGK CKVAINWSGG WHHAKKDEAS 150
    GFCYLNDAVL GILRLRRKFD RILYVDLDLH HGDGVEDAFS FTSKVMTVSL 200
    HKFSPGFFPG TGDMSDVGLG KGRYYSVNVP IQDGIQDEKY YHICESVLKE 250
    VYQAFNPKAV VLQLGADTIA GDPMCSFNMT PVGIGKCLKY VLQWQLATLI 300
    LGGGGYNLAN TARCWTYLTG VILGKTLSSE IPDHEFFTAY GPDYVLEITP 350
    SCRPDRNEPH RIQQILNYIK GNLKHVV 377
    Length:377
    Mass (Da):41,754
    Last modified:May 20, 2008 - v1
    Checksum:iAC7D7A3114663505
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC162023 mRNA. Translation: AAI62023.1.
    RefSeqiNP_001119845.2. NM_001126373.2.
    XP_003752118.1. XM_003752070.2.
    UniGeneiRn.208476.

    Genome annotation databases

    EnsembliENSRNOT00000004224; ENSRNOP00000004224; ENSRNOG00000003122.
    GeneIDi100911968.
    363481.
    KEGGirno:100911968.
    rno:363481.
    UCSCiRGD:1562895. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC162023 mRNA. Translation: AAI62023.1 .
    RefSeqi NP_001119845.2. NM_001126373.2.
    XP_003752118.1. XM_003752070.2.
    UniGenei Rn.208476.

    3D structure databases

    ProteinModelPortali B1WC68.
    SMRi B1WC68. Positions 14-377.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 10116.ENSRNOP00000004224.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSRNOT00000004224 ; ENSRNOP00000004224 ; ENSRNOG00000003122 .
    GeneIDi 100911968.
    363481.
    KEGGi rno:100911968.
    rno:363481.
    UCSCi RGD:1562895. rat.

    Organism-specific databases

    CTDi 55869.
    RGDi 1562895. Hdac8.

    Phylogenomic databases

    eggNOGi COG0123.
    GeneTreei ENSGT00530000062889.
    HOGENOMi HOG000225180.
    HOVERGENi HBG057112.
    KOi K11405.
    OMAi CLIDGKC.
    OrthoDBi EOG722J95.
    PhylomeDBi B1WC68.
    TreeFami TF106175.

    Enzyme and pathway databases

    Reactomei REACT_194781. Separation of Sister Chromatids.
    REACT_198611. Resolution of Sister Chromatid Cohesion.

    Miscellaneous databases

    NextBioi 683449.

    Gene expression databases

    Genevestigatori B1WC68.

    Family and domain databases

    Gene3Di 3.40.800.20. 1 hit.
    InterProi IPR000286. His_deacetylse.
    IPR003084. His_deacetylse_1.
    IPR023801. His_deacetylse_dom.
    [Graphical view ]
    PANTHERi PTHR10625. PTHR10625. 1 hit.
    Pfami PF00850. Hist_deacetyl. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF037913. His_deacetylse_1. 1 hit.
    PRINTSi PR01270. HDASUPER.
    PR01271. HISDACETLASE.
    ProtoNeti Search...

    Publicationsi

    1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Spleen.

    Entry informationi

    Entry nameiHDAC8_RAT
    AccessioniPrimary (citable) accession number: B1WC68
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 24, 2009
    Last sequence update: May 20, 2008
    Last modified: October 1, 2014
    This is version 45 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3