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B1WC68 (HDAC8_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 42. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Histone deacetylase 8

Short name=HD8
EC=3.5.1.98
Gene names
Name:Hdac8
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length377 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Also involved in the deacetylation of cohesin complex protein SMC3 regulating release of cohesin complexes from chromatin. May play a role in smooth muscle cell contractility By similarity.

Catalytic activity

Hydrolysis of an N(6)-acetyl-lysine residue of a histone to yield a deacetylated histone.

Cofactor

Binds 1 divalent metal cation per subunit.

Enzyme regulation

Its activity is inhibited by trichostatin A (TSA) and butyrate, 2 well known histone deacetylase inhibitors By similarity.

Subunit structure

Interacts with CBFA2T3. Interacts with phosphorylated SMG5/EST1B; this interaction protects SMG5 from ubiquitin-mediated degradation. Associates with alpha-SMA (smooth muscle alpha-actin) By similarity.

Subcellular location

Nucleus By similarity. Cytoplasm By similarity.

Post-translational modification

Phosphorylated by PKA on serine 39. Phosphorylation reduces deacetylase activity observed preferentially on histones H3 and H4 By similarity.

Sequence similarities

Belongs to the histone deacetylase family. HD type 1 subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 377377Histone deacetylase 8
PRO_0000389508

Regions

Region14 – 324311Histone deacetylase By similarity

Sites

Active site1431Proton acceptor By similarity
Metal binding1781Divalent metal cation By similarity
Metal binding1801Divalent metal cation By similarity
Metal binding2671Divalent metal cation By similarity
Binding site1011Substrate By similarity
Binding site1511Substrate; via carbonyl oxygen By similarity
Binding site3061Substrate By similarity

Amino acid modifications

Modified residue391Phosphoserine By similarity

Sequences

Sequence LengthMass (Da)Tools
B1WC68 [UniParc].

Last modified May 20, 2008. Version 1.
Checksum: AC7D7A3114663505

FASTA37741,754
        10         20         30         40         50         60 
MEIPEEPANS GHSLPPVYIY SPEYVSICDS LVKVPKRASM VHSLIEAYAL HKQMRIVKPK 

        70         80         90        100        110        120 
VASMEEMATF HTDAYLQHLQ KVSQEGDEDH PDSIEYGLGY DCPATEGIFD YAAAIGGGTI 

       130        140        150        160        170        180 
TAAQCLIDGK CKVAINWSGG WHHAKKDEAS GFCYLNDAVL GILRLRRKFD RILYVDLDLH 

       190        200        210        220        230        240 
HGDGVEDAFS FTSKVMTVSL HKFSPGFFPG TGDMSDVGLG KGRYYSVNVP IQDGIQDEKY 

       250        260        270        280        290        300 
YHICESVLKE VYQAFNPKAV VLQLGADTIA GDPMCSFNMT PVGIGKCLKY VLQWQLATLI 

       310        320        330        340        350        360 
LGGGGYNLAN TARCWTYLTG VILGKTLSSE IPDHEFFTAY GPDYVLEITP SCRPDRNEPH 

       370 
RIQQILNYIK GNLKHVV 

« Hide

References

[1]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Spleen.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BC162023 mRNA. Translation: AAI62023.1.
RefSeqNP_001119845.2. NM_001126373.2.
XP_003752118.1. XM_003752070.2.
UniGeneRn.208476.

3D structure databases

ProteinModelPortalB1WC68.
SMRB1WC68. Positions 14-377.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING10116.ENSRNOP00000004224.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSRNOT00000004224; ENSRNOP00000004224; ENSRNOG00000003122.
GeneID100911968.
363481.
KEGGrno:100911968.
rno:363481.
UCSCRGD:1562895. rat.

Organism-specific databases

CTD55869.
RGD1562895. Hdac8.

Phylogenomic databases

eggNOGCOG0123.
GeneTreeENSGT00530000062889.
HOGENOMHOG000225180.
HOVERGENHBG057112.
KOK11405.
OMAKCKVAIN.
OrthoDBEOG722J95.
PhylomeDBB1WC68.
TreeFamTF106175.

Gene expression databases

GenevestigatorB1WC68.

Family and domain databases

Gene3D3.40.800.20. 1 hit.
InterProIPR000286. His_deacetylse.
IPR003084. His_deacetylse_1.
IPR023801. His_deacetylse_dom.
[Graphical view]
PANTHERPTHR10625. PTHR10625. 1 hit.
PfamPF00850. Hist_deacetyl. 1 hit.
[Graphical view]
PIRSFPIRSF037913. His_deacetylse_1. 1 hit.
PRINTSPR01270. HDASUPER.
PR01271. HISDACETLASE.
ProtoNetSearch...

Other

NextBio683449.

Entry information

Entry nameHDAC8_RAT
AccessionPrimary (citable) accession number: B1WC68
Entry history
Integrated into UniProtKB/Swiss-Prot: November 24, 2009
Last sequence update: May 20, 2008
Last modified: April 16, 2014
This is version 42 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families