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B1WC68

- HDAC8_RAT

UniProt

B1WC68 - HDAC8_RAT

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Protein
Histone deacetylase 8
Gene
Hdac8
Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at transcript leveli

Functioni

Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Also involved in the deacetylation of cohesin complex protein SMC3 regulating release of cohesin complexes from chromatin. May play a role in smooth muscle cell contractility By similarity.

Catalytic activityi

Hydrolysis of an N(6)-acetyl-lysine residue of a histone to yield a deacetylated histone.

Cofactori

Binds 1 divalent metal cation per subunit.

Enzyme regulationi

Its activity is inhibited by trichostatin A (TSA) and butyrate, 2 well known histone deacetylase inhibitors By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei101 – 1011Substrate By similarity
Active sitei143 – 1431Proton acceptor By similarity
Binding sitei151 – 1511Substrate; via carbonyl oxygen By similarity
Metal bindingi178 – 1781Divalent metal cation By similarity
Metal bindingi180 – 1801Divalent metal cation By similarity
Metal bindingi267 – 2671Divalent metal cation By similarity
Binding sitei306 – 3061Substrate By similarity

GO - Molecular functioni

  1. NAD-dependent histone deacetylase activity (H3-K14 specific) Source: UniProtKB-EC
  2. NAD-dependent histone deacetylase activity (H3-K18 specific) Source: UniProtKB-EC
  3. NAD-dependent histone deacetylase activity (H3-K9 specific) Source: UniProtKB-EC
  4. NAD-dependent histone deacetylase activity (H4-K16 specific) Source: UniProtKB-EC
  5. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. regulation of cohesin localization to chromatin Source: UniProtKB
  2. regulation of transcription, DNA-templated Source: UniProtKB-KW
  3. sister chromatid cohesion Source: UniProtKB
  4. transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Chromatin regulator, Hydrolase, Repressor

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

Metal-binding

Enzyme and pathway databases

ReactomeiREACT_194781. Separation of Sister Chromatids.
REACT_198611. Resolution of Sister Chromatid Cohesion.

Names & Taxonomyi

Protein namesi
Recommended name:
Histone deacetylase 8 (EC:3.5.1.98)
Short name:
HD8
Gene namesi
Name:Hdac8
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Chromosome X

Organism-specific databases

RGDi1562895. Hdac8.

Subcellular locationi

Nucleus By similarity. Cytoplasm By similarity

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
  2. nucleus Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 377377Histone deacetylase 8
PRO_0000389508Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei39 – 391Phosphoserine By similarity

Post-translational modificationi

Phosphorylated by PKA on serine 39. Phosphorylation reduces deacetylase activity observed preferentially on histones H3 and H4 By similarity.

Keywords - PTMi

Phosphoprotein

Expressioni

Gene expression databases

GenevestigatoriB1WC68.

Interactioni

Subunit structurei

Interacts with CBFA2T3. Interacts with phosphorylated SMG5/EST1B; this interaction protects SMG5 from ubiquitin-mediated degradation. Associates with alpha-SMA (smooth muscle alpha-actin) By similarity.

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000004224.

Structurei

3D structure databases

ProteinModelPortaliB1WC68.
SMRiB1WC68. Positions 14-377.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni14 – 324311Histone deacetylase By similarity
Add
BLAST

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0123.
GeneTreeiENSGT00530000062889.
HOGENOMiHOG000225180.
HOVERGENiHBG057112.
KOiK11405.
OMAiCLIDGKC.
OrthoDBiEOG722J95.
PhylomeDBiB1WC68.
TreeFamiTF106175.

Family and domain databases

Gene3Di3.40.800.20. 1 hit.
InterProiIPR000286. His_deacetylse.
IPR003084. His_deacetylse_1.
IPR023801. His_deacetylse_dom.
[Graphical view]
PANTHERiPTHR10625. PTHR10625. 1 hit.
PfamiPF00850. Hist_deacetyl. 1 hit.
[Graphical view]
PIRSFiPIRSF037913. His_deacetylse_1. 1 hit.
PRINTSiPR01270. HDASUPER.
PR01271. HISDACETLASE.

Sequencei

Sequence statusi: Complete.

B1WC68-1 [UniParc]FASTAAdd to Basket

« Hide

MEIPEEPANS GHSLPPVYIY SPEYVSICDS LVKVPKRASM VHSLIEAYAL    50
HKQMRIVKPK VASMEEMATF HTDAYLQHLQ KVSQEGDEDH PDSIEYGLGY 100
DCPATEGIFD YAAAIGGGTI TAAQCLIDGK CKVAINWSGG WHHAKKDEAS 150
GFCYLNDAVL GILRLRRKFD RILYVDLDLH HGDGVEDAFS FTSKVMTVSL 200
HKFSPGFFPG TGDMSDVGLG KGRYYSVNVP IQDGIQDEKY YHICESVLKE 250
VYQAFNPKAV VLQLGADTIA GDPMCSFNMT PVGIGKCLKY VLQWQLATLI 300
LGGGGYNLAN TARCWTYLTG VILGKTLSSE IPDHEFFTAY GPDYVLEITP 350
SCRPDRNEPH RIQQILNYIK GNLKHVV 377
Length:377
Mass (Da):41,754
Last modified:May 20, 2008 - v1
Checksum:iAC7D7A3114663505
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BC162023 mRNA. Translation: AAI62023.1.
RefSeqiNP_001119845.2. NM_001126373.2.
XP_003752118.1. XM_003752070.2.
UniGeneiRn.208476.

Genome annotation databases

EnsembliENSRNOT00000004224; ENSRNOP00000004224; ENSRNOG00000003122.
GeneIDi100911968.
363481.
KEGGirno:100911968.
rno:363481.
UCSCiRGD:1562895. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BC162023 mRNA. Translation: AAI62023.1 .
RefSeqi NP_001119845.2. NM_001126373.2.
XP_003752118.1. XM_003752070.2.
UniGenei Rn.208476.

3D structure databases

ProteinModelPortali B1WC68.
SMRi B1WC68. Positions 14-377.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 10116.ENSRNOP00000004224.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSRNOT00000004224 ; ENSRNOP00000004224 ; ENSRNOG00000003122 .
GeneIDi 100911968.
363481.
KEGGi rno:100911968.
rno:363481.
UCSCi RGD:1562895. rat.

Organism-specific databases

CTDi 55869.
RGDi 1562895. Hdac8.

Phylogenomic databases

eggNOGi COG0123.
GeneTreei ENSGT00530000062889.
HOGENOMi HOG000225180.
HOVERGENi HBG057112.
KOi K11405.
OMAi CLIDGKC.
OrthoDBi EOG722J95.
PhylomeDBi B1WC68.
TreeFami TF106175.

Enzyme and pathway databases

Reactomei REACT_194781. Separation of Sister Chromatids.
REACT_198611. Resolution of Sister Chromatid Cohesion.

Miscellaneous databases

NextBioi 683449.

Gene expression databases

Genevestigatori B1WC68.

Family and domain databases

Gene3Di 3.40.800.20. 1 hit.
InterProi IPR000286. His_deacetylse.
IPR003084. His_deacetylse_1.
IPR023801. His_deacetylse_dom.
[Graphical view ]
PANTHERi PTHR10625. PTHR10625. 1 hit.
Pfami PF00850. Hist_deacetyl. 1 hit.
[Graphical view ]
PIRSFi PIRSF037913. His_deacetylse_1. 1 hit.
PRINTSi PR01270. HDASUPER.
PR01271. HISDACETLASE.
ProtoNeti Search...

Publicationsi

  1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Spleen.

Entry informationi

Entry nameiHDAC8_RAT
AccessioniPrimary (citable) accession number: B1WC68
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 24, 2009
Last sequence update: May 20, 2008
Last modified: September 3, 2014
This is version 44 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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