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Protein

Histone deacetylase 8

Gene

Hdac8

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Also involved in the deacetylation of cohesin complex protein SMC3 regulating release of cohesin complexes from chromatin. May play a role in smooth muscle cell contractility (By similarity).By similarity

Catalytic activityi

Hydrolysis of an N6-acetyl-lysine residue of a histone to yield a deacetylated histone.

Cofactori

a divalent metal cationNote: Binds 1 divalent metal cation per subunit.

Enzyme regulationi

Its activity is inhibited by trichostatin A (TSA) and butyrate, 2 well known histone deacetylase inhibitors.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei101SubstrateBy similarity1
Active sitei143Proton acceptorBy similarity1
Binding sitei151Substrate; via carbonyl oxygenBy similarity1
Metal bindingi178Divalent metal cationBy similarity1
Metal bindingi180Divalent metal cationBy similarity1
Metal bindingi267Divalent metal cationBy similarity1
Binding sitei306SubstrateBy similarity1

GO - Molecular functioni

GO - Biological processi

  • cellular response to forskolin Source: RGD
  • cellular response to trichostatin A Source: RGD
  • negative regulation of gene expression Source: RGD
  • negative regulation of histone H3-K9 acetylation Source: RGD
  • negative regulation of osteoblast differentiation Source: RGD
  • negative regulation of protein ubiquitination Source: Ensembl
  • regulation of cohesin loading Source: UniProtKB
  • regulation of protein stability Source: Ensembl
  • regulation of telomere maintenance Source: Ensembl
  • regulation of transcription, DNA-templated Source: UniProtKB-KW
  • sister chromatid cohesion Source: UniProtKB
  • transcription, DNA-templated Source: UniProtKB-KW

Keywordsi

Molecular functionChromatin regulator, Hydrolase, Repressor
Biological processTranscription, Transcription regulation
LigandMetal-binding

Enzyme and pathway databases

ReactomeiR-RNO-2467813 Separation of Sister Chromatids
R-RNO-2500257 Resolution of Sister Chromatid Cohesion

Names & Taxonomyi

Protein namesi
Recommended name:
Histone deacetylase 8 (EC:3.5.1.98)
Short name:
HD8
Gene namesi
Name:Hdac8
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi

Organism-specific databases

RGDi1562895 Hdac8

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003895081 – 377Histone deacetylase 8Add BLAST377

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei39PhosphoserineBy similarity1

Post-translational modificationi

Phosphorylated by PKA on serine 39. Phosphorylation reduces deacetylase activity observed preferentially on histones H3 and H4 (By similarity).By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiB1WC68
PeptideAtlasiB1WC68

PTM databases

iPTMnetiB1WC68
PhosphoSitePlusiB1WC68

Expressioni

Gene expression databases

BgeeiENSRNOG00000003122
GenevisibleiB1WC68 RN

Interactioni

Subunit structurei

Interacts with CBFA2T3. Interacts with phosphorylated SMG5/EST1B; this interaction protects SMG5 from ubiquitin-mediated degradation. Associates with alpha-SMA (smooth muscle alpha-actin) (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000004224

Structurei

3D structure databases

ProteinModelPortaliB1WC68
SMRiB1WC68
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni14 – 324Histone deacetylaseBy similarityAdd BLAST311

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG1342 Eukaryota
COG0123 LUCA
GeneTreeiENSGT00910000144047
HOGENOMiHOG000225180
HOVERGENiHBG057112
InParanoidiB1WC68
KOiK11405
OMAiCGYDANA
OrthoDBiEOG091G0A9R
PhylomeDBiB1WC68
TreeFamiTF106175

Family and domain databases

Gene3Di3.40.800.20, 1 hit
InterProiView protein in InterPro
IPR000286 His_deacetylse
IPR003084 His_deacetylse_1
IPR023801 His_deacetylse_dom
IPR037138 His_deacetylse_dom_sf
IPR023696 Ureohydrolase_dom_sf
PANTHERiPTHR10625 PTHR10625, 1 hit
PfamiView protein in Pfam
PF00850 Hist_deacetyl, 1 hit
PIRSFiPIRSF037913 His_deacetylse_1, 1 hit
PRINTSiPR01270 HDASUPER
PR01271 HISDACETLASE
SUPFAMiSSF52768 SSF52768, 1 hit

Sequencei

Sequence statusi: Complete.

B1WC68-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEIPEEPANS GHSLPPVYIY SPEYVSICDS LVKVPKRASM VHSLIEAYAL
60 70 80 90 100
HKQMRIVKPK VASMEEMATF HTDAYLQHLQ KVSQEGDEDH PDSIEYGLGY
110 120 130 140 150
DCPATEGIFD YAAAIGGGTI TAAQCLIDGK CKVAINWSGG WHHAKKDEAS
160 170 180 190 200
GFCYLNDAVL GILRLRRKFD RILYVDLDLH HGDGVEDAFS FTSKVMTVSL
210 220 230 240 250
HKFSPGFFPG TGDMSDVGLG KGRYYSVNVP IQDGIQDEKY YHICESVLKE
260 270 280 290 300
VYQAFNPKAV VLQLGADTIA GDPMCSFNMT PVGIGKCLKY VLQWQLATLI
310 320 330 340 350
LGGGGYNLAN TARCWTYLTG VILGKTLSSE IPDHEFFTAY GPDYVLEITP
360 370
SCRPDRNEPH RIQQILNYIK GNLKHVV
Length:377
Mass (Da):41,754
Last modified:May 20, 2008 - v1
Checksum:iAC7D7A3114663505
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC162023 mRNA Translation: AAI62023.1
RefSeqiNP_001119845.2, NM_001126373.2
UniGeneiRn.208476

Genome annotation databases

EnsembliENSRNOT00000004224; ENSRNOP00000004224; ENSRNOG00000003122
GeneIDi363481
KEGGirno:363481
UCSCiRGD:1562895 rat

Entry informationi

Entry nameiHDAC8_RAT
AccessioniPrimary (citable) accession number: B1WC68
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 24, 2009
Last sequence update: May 20, 2008
Last modified: May 23, 2018
This is version 72 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

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