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Protein

Metallophosphoesterase MPPED2

Gene

Mpped2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Displays low metallophosphoesterase activity (in vitro). May play a role in the development of the nervous system.1 Publication

Cofactori

Mn2+1 Publication, Co2+1 Publication

Kineticsi

  1. KM=10 mM for p-nitrophenyl phenyphosphonate1 Publication
  2. KM=1.5 mM for manganese1 Publication
  1. Vmax=8 µmol/min/mg enzyme with p-nitrophenyl phenyphosphonate as substrate1 Publication

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

Cobalt, Manganese, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Metallophosphoesterase MPPED2 (EC:3.1.-.-)
Alternative name(s):
239FB
Fetal brain protein 239 homolog
Metallophosphoesterase domain-containing protein 2
Gene namesi
Name:Mpped2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 3

Organism-specific databases

RGDi735060. Mpped2.

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi65 – 651D → A: Loss of phosphodiesterase activity. 1 Publication
Mutagenesisi86 – 861D → A: Loss of phosphodiesterase activity. 1 Publication
Mutagenesisi117 – 1171N → A: Loss of phosphodiesterase activity. 1 Publication
Mutagenesisi118 – 1181H → A: Loss of phosphodiesterase activity. 1 Publication
Mutagenesisi252 – 2521G → H: Increased affinity for manganese. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 294294Metallophosphoesterase MPPED2PRO_0000386546Add
BLAST

Proteomic databases

PaxDbiB1WBP0.

Expressioni

Tissue specificityi

Expressed in fetal brain.1 Publication

Gene expression databases

GenevisibleiB1WBP0. RN.

Interactioni

Subunit structurei

Homodimer.

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000062716.

Structurei

Secondary structure

1
294
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Turni17 – 204Combined sources
Helixi22 – 3312Combined sources
Turni38 – 403Combined sources
Beta strandi41 – 433Combined sources
Beta strandi57 – 648Combined sources
Beta strandi79 – 835Combined sources
Beta strandi88 – 903Combined sources
Helixi93 – 10412Combined sources
Beta strandi109 – 1135Combined sources
Helixi120 – 1223Combined sources
Helixi124 – 1307Combined sources
Helixi135 – 1373Combined sources
Helixi139 – 1424Combined sources
Helixi146 – 1494Combined sources
Turni150 – 1523Combined sources
Helixi153 – 1553Combined sources
Beta strandi159 – 1613Combined sources
Beta strandi163 – 1697Combined sources
Beta strandi172 – 1776Combined sources
Beta strandi183 – 1853Combined sources
Helixi194 – 2007Combined sources
Beta strandi208 – 2147Combined sources
Helixi224 – 2263Combined sources
Helixi233 – 2419Combined sources
Beta strandi246 – 2505Combined sources
Helixi254 – 2563Combined sources
Beta strandi258 – 2614Combined sources
Beta strandi266 – 2694Combined sources
Beta strandi284 – 2896Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3RL3X-ray1.42A1-294[»]
3RL4X-ray1.29A1-294[»]
3RL5X-ray1.26A1-294[»]
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiB1WBP0.

Family & Domainsi

Sequence similaritiesi

Belongs to the UPF0046 family.Curated

Phylogenomic databases

eggNOGiKOG3947. Eukaryota.
ENOG4111K47. LUCA.
GeneTreeiENSGT00390000007681.
HOGENOMiHOG000023302.
InParanoidiB1WBP0.
OMAiHMGDFTE.
OrthoDBiEOG7G4QFH.
PhylomeDBiB1WBP0.
TreeFamiTF314305.

Family and domain databases

Gene3Di3.60.21.10. 2 hits.
InterProiIPR024201. Calcineurin-like_Pesterase.
IPR004843. Calcineurin-like_PHP_apaH.
IPR029052. Metallo-depent_PP-like.
[Graphical view]
PfamiPF00149. Metallophos. 1 hit.
[Graphical view]
PIRSFiPIRSF035808. Pdiesterase_Brain_239. 1 hit.
SUPFAMiSSF56300. SSF56300. 1 hit.

Sequencei

Sequence statusi: Complete.

B1WBP0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAHGIPSQGK VTITVDEYSS NPTQAFTHYN INQSRFQPPH VHMVDPIPYD
60 70 80 90 100
TPKPAGHTRF VCISDTHSRT DGIQMPYGDI LLHTGDFTEL GLPSEVKKFN
110 120 130 140 150
DWLGNLPYEY KIVIAGNHEL TFDKEFMADL VKQDYYRFPS VSKLKPEDFD
160 170 180 190 200
NVQSLLTNSI YLQDSEVTVK GFRIYGAPWT PWFNGWGFNL PRGQSLLDKW
210 220 230 240 250
NLIPEGTDIL MTHGPPLGFR DWVPKELQRV GCVELLNTVQ RRVRPKLHVF
260 270 280 290
GGIHEGYGTM TDGYTTYINA STCTVSFQPT NPPIIFDLPN PQGS
Length:294
Mass (Da):33,336
Last modified:May 20, 2008 - v1
Checksum:iFEAE6DD0537C21E5
GO

Sequence cautioni

The sequence AAM09960.1 differs from that shown. Reason: Erroneous termination at position 257. Translated as Tyr.Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti123 – 1231D → E in AAM09960 (Ref. 3) Curated
Sequence conflicti159 – 1591S → C in AAM09960 (Ref. 3) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CH473949 Genomic DNA. Translation: EDL79739.1.
CH473949 Genomic DNA. Translation: EDL79741.1.
CH473949 Genomic DNA. Translation: EDL79742.1.
BC161828 mRNA. Translation: AAI61828.1.
AY093422 mRNA. Translation: AAM09960.1. Sequence problems.
RefSeqiNP_942073.2. NM_198778.2.
XP_006234742.1. XM_006234680.2.
XP_006234743.1. XM_006234681.2.
XP_006234744.1. XM_006234682.2.
XP_006234745.1. XM_006234683.2.
XP_008760326.1. XM_008762104.1.
UniGeneiRn.206970.

Genome annotation databases

EnsembliENSRNOT00000065168; ENSRNOP00000062716; ENSRNOG00000004863.
ENSRNOT00000080416; ENSRNOP00000073709; ENSRNOG00000004863.
GeneIDi362185.
KEGGirno:362185.
UCSCiRGD:735060. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CH473949 Genomic DNA. Translation: EDL79739.1.
CH473949 Genomic DNA. Translation: EDL79741.1.
CH473949 Genomic DNA. Translation: EDL79742.1.
BC161828 mRNA. Translation: AAI61828.1.
AY093422 mRNA. Translation: AAM09960.1. Sequence problems.
RefSeqiNP_942073.2. NM_198778.2.
XP_006234742.1. XM_006234680.2.
XP_006234743.1. XM_006234681.2.
XP_006234744.1. XM_006234682.2.
XP_006234745.1. XM_006234683.2.
XP_008760326.1. XM_008762104.1.
UniGeneiRn.206970.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3RL3X-ray1.42A1-294[»]
3RL4X-ray1.29A1-294[»]
3RL5X-ray1.26A1-294[»]
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000062716.

Proteomic databases

PaxDbiB1WBP0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000065168; ENSRNOP00000062716; ENSRNOG00000004863.
ENSRNOT00000080416; ENSRNOP00000073709; ENSRNOG00000004863.
GeneIDi362185.
KEGGirno:362185.
UCSCiRGD:735060. rat.

Organism-specific databases

CTDi744.
RGDi735060. Mpped2.

Phylogenomic databases

eggNOGiKOG3947. Eukaryota.
ENOG4111K47. LUCA.
GeneTreeiENSGT00390000007681.
HOGENOMiHOG000023302.
InParanoidiB1WBP0.
OMAiHMGDFTE.
OrthoDBiEOG7G4QFH.
PhylomeDBiB1WBP0.
TreeFamiTF314305.

Miscellaneous databases

EvolutionaryTraceiB1WBP0.
PROiB1WBP0.

Gene expression databases

GenevisibleiB1WBP0. RN.

Family and domain databases

Gene3Di3.60.21.10. 2 hits.
InterProiIPR024201. Calcineurin-like_Pesterase.
IPR004843. Calcineurin-like_PHP_apaH.
IPR029052. Metallo-depent_PP-like.
[Graphical view]
PfamiPF00149. Metallophos. 1 hit.
[Graphical view]
PIRSFiPIRSF035808. Pdiesterase_Brain_239. 1 hit.
SUPFAMiSSF56300. SSF56300. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Embryonic heart.
  3. "Molecular cloning and characterization of a rat novel A18 hnRNP protein binding gene."
    Chen D., Saugstad J., Henshall D., Minami M., Simon R.P.
    Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-283.
    Tissue: Hippocampus.
  4. "Characterization of an evolutionarily conserved metallophosphoesterase that is expressed in the fetal brain and associated with the WAGR syndrome."
    Tyagi R., Shenoy A.R., Visweswariah S.S.
    J. Biol. Chem. 284:5217-5228(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, COFACTOR, BIOPHYSICOCHEMICAL PROPERTIES, HOMODIMERIZATION, TISSUE SPECIFICITY, MUTAGENESIS OF ASP-65; ASP-86; ASN-117; HIS-118 AND GLY-252.

Entry informationi

Entry nameiMPPD2_RAT
AccessioniPrimary (citable) accession number: B1WBP0
Secondary accession number(s): Q8CFG1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 13, 2009
Last sequence update: May 20, 2008
Last modified: June 8, 2016
This is version 52 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

This protein has a low affinity for cofactor and demonstrates very restricted substrate specificity. The enzyme may need additional interacting proteins to show full activity, or may be losing its activity and acquiring the role of a scaffolding protein.

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Uncharacterized protein families (UPF)
    List of uncharacterized protein family (UPF) entries

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.