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Protein

Metallophosphoesterase MPPED2

Gene

Mpped2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Displays low metallophosphoesterase activity (in vitro). May play a role in the development of the nervous system.1 Publication

Cofactori

Mn2+1 Publication, Co2+1 Publication

Kineticsi

  1. KM=10 mM for p-nitrophenyl phenyphosphonate1 Publication
  2. KM=1.5 mM for manganese1 Publication
  1. Vmax=8 µmol/min/mg enzyme with p-nitrophenyl phenyphosphonate as substrate1 Publication

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

Cobalt, Manganese, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Metallophosphoesterase MPPED2 (EC:3.1.-.-)
Alternative name(s):
239FB
Fetal brain protein 239 homolog
Metallophosphoesterase domain-containing protein 2
Gene namesi
Name:Mpped2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 3

Organism-specific databases

RGDi735060. Mpped2.

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi65D → A: Loss of phosphodiesterase activity. 1 Publication1
Mutagenesisi86D → A: Loss of phosphodiesterase activity. 1 Publication1
Mutagenesisi117N → A: Loss of phosphodiesterase activity. 1 Publication1
Mutagenesisi118H → A: Loss of phosphodiesterase activity. 1 Publication1
Mutagenesisi252G → H: Increased affinity for manganese. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003865461 – 294Metallophosphoesterase MPPED2Add BLAST294

Proteomic databases

PaxDbiB1WBP0.

Expressioni

Tissue specificityi

Expressed in fetal brain.1 Publication

Gene expression databases

BgeeiENSRNOG00000004863.
GenevisibleiB1WBP0. RN.

Interactioni

Subunit structurei

Homodimer.

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000062716.

Structurei

Secondary structure

1294
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Turni17 – 20Combined sources4
Helixi22 – 33Combined sources12
Turni38 – 40Combined sources3
Beta strandi41 – 43Combined sources3
Beta strandi57 – 64Combined sources8
Beta strandi79 – 83Combined sources5
Beta strandi88 – 90Combined sources3
Helixi93 – 104Combined sources12
Beta strandi109 – 113Combined sources5
Helixi120 – 122Combined sources3
Helixi124 – 130Combined sources7
Helixi135 – 137Combined sources3
Helixi139 – 142Combined sources4
Helixi146 – 149Combined sources4
Turni150 – 152Combined sources3
Helixi153 – 155Combined sources3
Beta strandi159 – 161Combined sources3
Beta strandi163 – 169Combined sources7
Beta strandi172 – 177Combined sources6
Beta strandi183 – 185Combined sources3
Helixi194 – 200Combined sources7
Beta strandi208 – 214Combined sources7
Helixi224 – 226Combined sources3
Helixi233 – 241Combined sources9
Beta strandi246 – 250Combined sources5
Helixi254 – 256Combined sources3
Beta strandi258 – 261Combined sources4
Beta strandi266 – 269Combined sources4
Beta strandi284 – 289Combined sources6

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3RL3X-ray1.42A1-294[»]
3RL4X-ray1.29A1-294[»]
3RL5X-ray1.26A1-294[»]
SMRiB1WBP0.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiB1WBP0.

Family & Domainsi

Sequence similaritiesi

Belongs to the UPF0046 family.Curated

Phylogenomic databases

eggNOGiKOG3947. Eukaryota.
ENOG4111K47. LUCA.
GeneTreeiENSGT00390000007681.
HOGENOMiHOG000023302.
InParanoidiB1WBP0.
OMAiHMGDFTE.
PhylomeDBiB1WBP0.
TreeFamiTF314305.

Family and domain databases

Gene3Di3.60.21.10. 2 hits.
InterProiIPR024201. Calcineurin-like_Pesterase.
IPR004843. Calcineurin-like_PHP_ApaH.
IPR029052. Metallo-depent_PP-like.
[Graphical view]
PfamiPF00149. Metallophos. 1 hit.
[Graphical view]
PIRSFiPIRSF035808. Pdiesterase_Brain_239. 1 hit.
SUPFAMiSSF56300. SSF56300. 1 hit.

Sequencei

Sequence statusi: Complete.

B1WBP0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAHGIPSQGK VTITVDEYSS NPTQAFTHYN INQSRFQPPH VHMVDPIPYD
60 70 80 90 100
TPKPAGHTRF VCISDTHSRT DGIQMPYGDI LLHTGDFTEL GLPSEVKKFN
110 120 130 140 150
DWLGNLPYEY KIVIAGNHEL TFDKEFMADL VKQDYYRFPS VSKLKPEDFD
160 170 180 190 200
NVQSLLTNSI YLQDSEVTVK GFRIYGAPWT PWFNGWGFNL PRGQSLLDKW
210 220 230 240 250
NLIPEGTDIL MTHGPPLGFR DWVPKELQRV GCVELLNTVQ RRVRPKLHVF
260 270 280 290
GGIHEGYGTM TDGYTTYINA STCTVSFQPT NPPIIFDLPN PQGS
Length:294
Mass (Da):33,336
Last modified:May 20, 2008 - v1
Checksum:iFEAE6DD0537C21E5
GO

Sequence cautioni

The sequence AAM09960 differs from that shown. Reason: Erroneous termination at position 257. Translated as Tyr.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti123D → E in AAM09960 (Ref. 3) Curated1
Sequence conflicti159S → C in AAM09960 (Ref. 3) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CH473949 Genomic DNA. Translation: EDL79739.1.
CH473949 Genomic DNA. Translation: EDL79741.1.
CH473949 Genomic DNA. Translation: EDL79742.1.
BC161828 mRNA. Translation: AAI61828.1.
AY093422 mRNA. Translation: AAM09960.1. Sequence problems.
RefSeqiNP_942073.2. NM_198778.2.
XP_006234742.1. XM_006234680.3.
XP_006234743.1. XM_006234681.3.
XP_006234744.1. XM_006234682.3.
XP_006234745.1. XM_006234683.3.
XP_008760326.1. XM_008762104.2.
XP_017447394.1. XM_017591905.1.
XP_017447395.1. XM_017591906.1.
UniGeneiRn.206970.

Genome annotation databases

EnsembliENSRNOT00000065168; ENSRNOP00000062716; ENSRNOG00000004863.
ENSRNOT00000080416; ENSRNOP00000073709; ENSRNOG00000004863.
GeneIDi362185.
KEGGirno:362185.
UCSCiRGD:735060. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CH473949 Genomic DNA. Translation: EDL79739.1.
CH473949 Genomic DNA. Translation: EDL79741.1.
CH473949 Genomic DNA. Translation: EDL79742.1.
BC161828 mRNA. Translation: AAI61828.1.
AY093422 mRNA. Translation: AAM09960.1. Sequence problems.
RefSeqiNP_942073.2. NM_198778.2.
XP_006234742.1. XM_006234680.3.
XP_006234743.1. XM_006234681.3.
XP_006234744.1. XM_006234682.3.
XP_006234745.1. XM_006234683.3.
XP_008760326.1. XM_008762104.2.
XP_017447394.1. XM_017591905.1.
XP_017447395.1. XM_017591906.1.
UniGeneiRn.206970.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3RL3X-ray1.42A1-294[»]
3RL4X-ray1.29A1-294[»]
3RL5X-ray1.26A1-294[»]
SMRiB1WBP0.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000062716.

Proteomic databases

PaxDbiB1WBP0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000065168; ENSRNOP00000062716; ENSRNOG00000004863.
ENSRNOT00000080416; ENSRNOP00000073709; ENSRNOG00000004863.
GeneIDi362185.
KEGGirno:362185.
UCSCiRGD:735060. rat.

Organism-specific databases

CTDi744.
RGDi735060. Mpped2.

Phylogenomic databases

eggNOGiKOG3947. Eukaryota.
ENOG4111K47. LUCA.
GeneTreeiENSGT00390000007681.
HOGENOMiHOG000023302.
InParanoidiB1WBP0.
OMAiHMGDFTE.
PhylomeDBiB1WBP0.
TreeFamiTF314305.

Miscellaneous databases

EvolutionaryTraceiB1WBP0.
PROiB1WBP0.

Gene expression databases

BgeeiENSRNOG00000004863.
GenevisibleiB1WBP0. RN.

Family and domain databases

Gene3Di3.60.21.10. 2 hits.
InterProiIPR024201. Calcineurin-like_Pesterase.
IPR004843. Calcineurin-like_PHP_ApaH.
IPR029052. Metallo-depent_PP-like.
[Graphical view]
PfamiPF00149. Metallophos. 1 hit.
[Graphical view]
PIRSFiPIRSF035808. Pdiesterase_Brain_239. 1 hit.
SUPFAMiSSF56300. SSF56300. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiMPPD2_RAT
AccessioniPrimary (citable) accession number: B1WBP0
Secondary accession number(s): Q8CFG1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 13, 2009
Last sequence update: May 20, 2008
Last modified: November 30, 2016
This is version 55 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

This protein has a low affinity for cofactor and demonstrates very restricted substrate specificity. The enzyme may need additional interacting proteins to show full activity, or may be losing its activity and acquiring the role of a scaffolding protein.

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Uncharacterized protein families (UPF)
    List of uncharacterized protein family (UPF) entries

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.