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B1WBD7

- UBP20_XENLA

UniProt

B1WBD7 - UBP20_XENLA

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Protein

Ubiquitin carboxyl-terminal hydrolase 20

Gene

usp20

Organism
Xenopus laevis (African clawed frog)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli

Functioni

Deubiquitinating enzyme involved in beta-2 adrenergic receptor (adrb2) recycling. Acts as a regulator of G-protein coupled receptor (GPCR) signaling by mediating the deubiquitination beta-2 adrenergic receptor (adrb2). Plays a central role in adrb2 recycling and resensitization after prolonged agonist stimulation by constitutively binding adrb2, mediating deubiquitination of adrb2 and inhibiting lysosomal trafficking of adrb2. Mediates deubiquitination of both 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains (By similarity).By similarity

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei110 – 1101NucleophilePROSITE-ProRule annotation
Active sitei569 – 5691Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri28 – 9265UBP-typePROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  1. cysteine-type endopeptidase activity Source: UniProtKB
  2. ubiquitin-specific protease activity Source: InterPro
  3. ubiquitin thiolesterase activity Source: UniProtKB
  4. zinc ion binding Source: InterPro

GO - Biological processi

  1. endocytosis Source: UniProtKB-KW
  2. protein deubiquitination Source: UniProtKB
  3. protein K48-linked deubiquitination Source: UniProtKB
  4. protein K63-linked deubiquitination Source: UniProtKB
  5. regulation of G-protein coupled receptor protein signaling pathway Source: UniProtKB
  6. ubiquitin-dependent protein catabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Keywords - Biological processi

Endocytosis, Ubl conjugation pathway

Keywords - Ligandi

Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin carboxyl-terminal hydrolase 20 (EC:3.4.19.12)
Alternative name(s):
Deubiquitinating enzyme 20
Ubiquitin thioesterase 20
Ubiquitin-specific-processing protease 20
Gene namesi
Name:usp20
OrganismiXenopus laevis (African clawed frog)
Taxonomic identifieri8355 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraPipoideaPipidaeXenopodinaeXenopusXenopus

Organism-specific databases

XenbaseiXB-GENE-966533. usp20.

Subcellular locationi

GO - Cellular componenti

  1. centrosome Source: UniProtKB
  2. cytoplasm Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 840840Ubiquitin carboxyl-terminal hydrolase 20PRO_0000390421Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliB1WBD7.
SMRiB1WBD7. Positions 6-67.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini101 – 611511USPAdd
BLAST
Domaini613 – 70694DUSP 1PROSITE-ProRule annotationAdd
BLAST
Domaini715 – 818104DUSP 2PROSITE-ProRule annotationAdd
BLAST

Domaini

The UBP-type zinc finger binds 3 zinc ions. However, it does not bind ubiquitin, probably because the conserved Arg in position 55 is replaced by a Glu residue (By similarity).By similarity

Sequence similaritiesi

Contains 2 DUSP domains.PROSITE-ProRule annotation
Contains 1 UBP-type zinc finger.PROSITE-ProRule annotation
Contains 1 USP domain.Curated

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri28 – 9265UBP-typePROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

HOVERGENiHBG054196.
KOiK11848.

Family and domain databases

Gene3Di3.30.2230.10. 2 hits.
3.30.40.10. 1 hit.
InterProiIPR006615. Pept_C19_DUSP.
IPR018200. Pept_C19ubi-hydrolase_C_CS.
IPR001394. Peptidase_C19_UCH.
IPR028889. UCH/PAN2.
IPR013083. Znf_RING/FYVE/PHD.
IPR001607. Znf_UBP.
[Graphical view]
PfamiPF06337. DUSP. 2 hits.
PF00443. UCH. 1 hit.
PF02148. zf-UBP. 1 hit.
[Graphical view]
SMARTiSM00695. DUSP. 2 hits.
[Graphical view]
SUPFAMiSSF143791. SSF143791. 2 hits.
PROSITEiPS51283. DUSP. 2 hits.
PS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
PS50271. ZF_UBP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B1WBD7-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MADAEDFCPH LDSIGEVTKE DLILKSKGTC ESCGVGGPNL WACLQDGCQS
60 70 80 90 100
VGCGESYADH STLHAQDFPS PAHPLKSVPI AVGDDGESES DEDDIKPRGL
110 120 130 140 150
TGMKNIGNSC YMNAALQALS NCPPLTQFFL ECGGLVRTDK KPALCKSYQK
160 170 180 190 200
LVSELWHKKR PSYVVPSSLY HGIKLINPLF RGYSQQDTQE FLRCLMDQLH
210 220 230 240 250
EELKEPILPE NQEQEEEERD DQREGERGGT TEEDFLSCDS GGEMGDGEGG
260 270 280 290 300
GGVGTLSEME LLIREEVGRG LSEKEKLKER KLSYCHRRTS SEQADEDADV
310 320 330 340 350
DTAMIPEPDN DAYMHCSSRS CSPHPVESIS KHSSTPPRSS PLRTAHSYVL
360 370 380 390 400
KKAQVLSGGK KRSEVRYRSV ISDIFDGSIL SLVQCLTCDR VSTTIETFQD
410 420 430 440 450
LSLPIPGKED LAKLHSTIHQ STVIKAGTCG DSYAAQGWLA FVMDYIRRFV
460 470 480 490 500
VSCIPSWFWG PMITLEDCLA AFFAADELKG DNMYSCERCK KLRNGVKYCK
510 520 530 540 550
VLRLPEVLCI HLKRFRHEVM YSFKIGSHVS FPLEGLNLRP FLAKECVSRI
560 570 580 590 600
TTYDLLAVIC HHGSASSGHY ISYCQNVING QWYEFDDQYV TEVHETVVQN
610 620 630 640 650
AEAYVLFYRK SSEEAERERQ KVVSLAAMKE SGLLQFYISR EWLNKFNTFA
660 670 680 690 700
EPGPISNQSF LCAHGGIPPN KYHYIDDLVV ILPQSVWEYL YNRFGGGPAV
710 720 730 740 750
NHLYVCSICQ VEIEALAKRR KTEIDTFIKL NKAFQAEEAP SVIYCISMQW
760 770 780 790 800
FREWEAFVKA KDSDPPGPID NSKVALTKSS GHVQLKQGAD YGQISEETWN
810 820 830 840
YLLNIYGGGP EIAIRQTVAQ YQDPEHLHGE QKIEAETRAG
Length:840
Mass (Da):94,092
Last modified:May 20, 2008 - v1
Checksum:iC75C9C8E2A85C350
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BC161716 mRNA. Translation: AAI61716.1.
RefSeqiNP_001121345.1. NM_001127873.1.
UniGeneiXl.16107.

Genome annotation databases

GeneIDi100158436.
KEGGixla:100158436.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BC161716 mRNA. Translation: AAI61716.1 .
RefSeqi NP_001121345.1. NM_001127873.1.
UniGenei Xl.16107.

3D structure databases

ProteinModelPortali B1WBD7.
SMRi B1WBD7. Positions 6-67.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 100158436.
KEGGi xla:100158436.

Organism-specific databases

CTDi 10868.
Xenbasei XB-GENE-966533. usp20.

Phylogenomic databases

HOVERGENi HBG054196.
KOi K11848.

Family and domain databases

Gene3Di 3.30.2230.10. 2 hits.
3.30.40.10. 1 hit.
InterProi IPR006615. Pept_C19_DUSP.
IPR018200. Pept_C19ubi-hydrolase_C_CS.
IPR001394. Peptidase_C19_UCH.
IPR028889. UCH/PAN2.
IPR013083. Znf_RING/FYVE/PHD.
IPR001607. Znf_UBP.
[Graphical view ]
Pfami PF06337. DUSP. 2 hits.
PF00443. UCH. 1 hit.
PF02148. zf-UBP. 1 hit.
[Graphical view ]
SMARTi SM00695. DUSP. 2 hits.
[Graphical view ]
SUPFAMi SSF143791. SSF143791. 2 hits.
PROSITEi PS51283. DUSP. 2 hits.
PS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
PS50271. ZF_UBP. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. NIH - Xenopus Gene Collection (XGC) project
    Submitted (APR-2008) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Ovary.

Entry informationi

Entry nameiUBP20_XENLA
AccessioniPrimary (citable) accession number: B1WBD7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 2009
Last sequence update: May 20, 2008
Last modified: October 29, 2014
This is version 39 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3