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B1WBD7

- UBP20_XENLA

UniProt

B1WBD7 - UBP20_XENLA

Protein

Ubiquitin carboxyl-terminal hydrolase 20

Gene

usp20

Organism
Xenopus laevis (African clawed frog)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 38 (01 Oct 2014)
      Sequence version 1 (20 May 2008)
      Previous versions | rss
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    Functioni

    Deubiquitinating enzyme involved in beta-2 adrenergic receptor (adrb2) recycling. Acts as a regulator of G-protein coupled receptor (GPCR) signaling by mediating the deubiquitination beta-2 adrenergic receptor (adrb2). Plays a central role in adrb2 recycling and resensitization after prolonged agonist stimulation by constitutively binding adrb2, mediating deubiquitination of adrb2 and inhibiting lysosomal trafficking of adrb2. Mediates deubiquitination of both 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains By similarity.By similarity

    Catalytic activityi

    Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei110 – 1101NucleophilePROSITE-ProRule annotation
    Active sitei569 – 5691Proton acceptorPROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri28 – 9265UBP-typePROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. cysteine-type endopeptidase activity Source: UniProtKB
    2. ubiquitin-specific protease activity Source: InterPro
    3. ubiquitin thiolesterase activity Source: UniProtKB
    4. zinc ion binding Source: InterPro

    GO - Biological processi

    1. endocytosis Source: UniProtKB-KW
    2. protein deubiquitination Source: UniProtKB
    3. protein K48-linked deubiquitination Source: UniProtKB
    4. protein K63-linked deubiquitination Source: UniProtKB
    5. regulation of G-protein coupled receptor protein signaling pathway Source: UniProtKB
    6. ubiquitin-dependent protein catabolic process Source: InterPro

    Keywords - Molecular functioni

    Hydrolase, Protease, Thiol protease

    Keywords - Biological processi

    Endocytosis, Ubl conjugation pathway

    Keywords - Ligandi

    Metal-binding, Zinc

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ubiquitin carboxyl-terminal hydrolase 20 (EC:3.4.19.12)
    Alternative name(s):
    Deubiquitinating enzyme 20
    Ubiquitin thioesterase 20
    Ubiquitin-specific-processing protease 20
    Gene namesi
    Name:usp20
    OrganismiXenopus laevis (African clawed frog)
    Taxonomic identifieri8355 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraPipoideaPipidaeXenopodinaeXenopusXenopus

    Organism-specific databases

    XenbaseiXB-GENE-966533. usp20.

    Subcellular locationi

    GO - Cellular componenti

    1. centrosome Source: UniProtKB
    2. perinuclear region of cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm, Cytoskeleton

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 840840Ubiquitin carboxyl-terminal hydrolase 20PRO_0000390421Add
    BLAST

    Structurei

    3D structure databases

    ProteinModelPortaliB1WBD7.
    SMRiB1WBD7. Positions 6-67.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini101 – 611511USPAdd
    BLAST
    Domaini613 – 70694DUSP 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini715 – 818104DUSP 2PROSITE-ProRule annotationAdd
    BLAST

    Domaini

    The UBP-type zinc finger binds 3 zinc ions. However, it does not bind ubiquitin, probably because the conserved Arg in position 55 is replaced by a Glu residue By similarity.By similarity

    Sequence similaritiesi

    Contains 2 DUSP domains.PROSITE-ProRule annotation
    Contains 1 UBP-type zinc finger.PROSITE-ProRule annotation
    Contains 1 USP domain.Curated

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri28 – 9265UBP-typePROSITE-ProRule annotationAdd
    BLAST

    Keywords - Domaini

    Repeat, Zinc-finger

    Phylogenomic databases

    HOVERGENiHBG054196.
    KOiK11848.

    Family and domain databases

    Gene3Di3.30.2230.10. 2 hits.
    3.30.40.10. 1 hit.
    InterProiIPR006615. Pept_C19_DUSP.
    IPR018200. Pept_C19ubi-hydrolase_C_CS.
    IPR001394. Peptidase_C19_UCH.
    IPR028889. UCH/PAN2.
    IPR013083. Znf_RING/FYVE/PHD.
    IPR001607. Znf_UBP.
    [Graphical view]
    PfamiPF06337. DUSP. 2 hits.
    PF00443. UCH. 1 hit.
    PF02148. zf-UBP. 1 hit.
    [Graphical view]
    SMARTiSM00695. DUSP. 2 hits.
    [Graphical view]
    SUPFAMiSSF143791. SSF143791. 2 hits.
    PROSITEiPS51283. DUSP. 2 hits.
    PS00972. USP_1. 1 hit.
    PS00973. USP_2. 1 hit.
    PS50235. USP_3. 1 hit.
    PS50271. ZF_UBP. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    B1WBD7-1 [UniParc]FASTAAdd to Basket

    « Hide

    MADAEDFCPH LDSIGEVTKE DLILKSKGTC ESCGVGGPNL WACLQDGCQS    50
    VGCGESYADH STLHAQDFPS PAHPLKSVPI AVGDDGESES DEDDIKPRGL 100
    TGMKNIGNSC YMNAALQALS NCPPLTQFFL ECGGLVRTDK KPALCKSYQK 150
    LVSELWHKKR PSYVVPSSLY HGIKLINPLF RGYSQQDTQE FLRCLMDQLH 200
    EELKEPILPE NQEQEEEERD DQREGERGGT TEEDFLSCDS GGEMGDGEGG 250
    GGVGTLSEME LLIREEVGRG LSEKEKLKER KLSYCHRRTS SEQADEDADV 300
    DTAMIPEPDN DAYMHCSSRS CSPHPVESIS KHSSTPPRSS PLRTAHSYVL 350
    KKAQVLSGGK KRSEVRYRSV ISDIFDGSIL SLVQCLTCDR VSTTIETFQD 400
    LSLPIPGKED LAKLHSTIHQ STVIKAGTCG DSYAAQGWLA FVMDYIRRFV 450
    VSCIPSWFWG PMITLEDCLA AFFAADELKG DNMYSCERCK KLRNGVKYCK 500
    VLRLPEVLCI HLKRFRHEVM YSFKIGSHVS FPLEGLNLRP FLAKECVSRI 550
    TTYDLLAVIC HHGSASSGHY ISYCQNVING QWYEFDDQYV TEVHETVVQN 600
    AEAYVLFYRK SSEEAERERQ KVVSLAAMKE SGLLQFYISR EWLNKFNTFA 650
    EPGPISNQSF LCAHGGIPPN KYHYIDDLVV ILPQSVWEYL YNRFGGGPAV 700
    NHLYVCSICQ VEIEALAKRR KTEIDTFIKL NKAFQAEEAP SVIYCISMQW 750
    FREWEAFVKA KDSDPPGPID NSKVALTKSS GHVQLKQGAD YGQISEETWN 800
    YLLNIYGGGP EIAIRQTVAQ YQDPEHLHGE QKIEAETRAG 840
    Length:840
    Mass (Da):94,092
    Last modified:May 20, 2008 - v1
    Checksum:iC75C9C8E2A85C350
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC161716 mRNA. Translation: AAI61716.1.
    RefSeqiNP_001121345.1. NM_001127873.1.
    UniGeneiXl.16107.

    Genome annotation databases

    GeneIDi100158436.
    KEGGixla:100158436.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC161716 mRNA. Translation: AAI61716.1 .
    RefSeqi NP_001121345.1. NM_001127873.1.
    UniGenei Xl.16107.

    3D structure databases

    ProteinModelPortali B1WBD7.
    SMRi B1WBD7. Positions 6-67.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 100158436.
    KEGGi xla:100158436.

    Organism-specific databases

    CTDi 10868.
    Xenbasei XB-GENE-966533. usp20.

    Phylogenomic databases

    HOVERGENi HBG054196.
    KOi K11848.

    Family and domain databases

    Gene3Di 3.30.2230.10. 2 hits.
    3.30.40.10. 1 hit.
    InterProi IPR006615. Pept_C19_DUSP.
    IPR018200. Pept_C19ubi-hydrolase_C_CS.
    IPR001394. Peptidase_C19_UCH.
    IPR028889. UCH/PAN2.
    IPR013083. Znf_RING/FYVE/PHD.
    IPR001607. Znf_UBP.
    [Graphical view ]
    Pfami PF06337. DUSP. 2 hits.
    PF00443. UCH. 1 hit.
    PF02148. zf-UBP. 1 hit.
    [Graphical view ]
    SMARTi SM00695. DUSP. 2 hits.
    [Graphical view ]
    SUPFAMi SSF143791. SSF143791. 2 hits.
    PROSITEi PS51283. DUSP. 2 hits.
    PS00972. USP_1. 1 hit.
    PS00973. USP_2. 1 hit.
    PS50235. USP_3. 1 hit.
    PS50271. ZF_UBP. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. NIH - Xenopus Gene Collection (XGC) project
      Submitted (APR-2008) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Ovary.

    Entry informationi

    Entry nameiUBP20_XENLA
    AccessioniPrimary (citable) accession number: B1WBD7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 15, 2009
    Last sequence update: May 20, 2008
    Last modified: October 1, 2014
    This is version 38 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Documents

    1. Peptidase families
      Classification of peptidase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3