Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

B1WBD7 (UBP20_XENLA) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 37. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Ubiquitin carboxyl-terminal hydrolase 20

EC=3.4.19.12
Alternative name(s):
Deubiquitinating enzyme 20
Ubiquitin thioesterase 20
Ubiquitin-specific-processing protease 20
Gene names
Name:usp20
OrganismXenopus laevis (African clawed frog)
Taxonomic identifier8355 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraPipoideaPipidaeXenopodinaeXenopusXenopus

Protein attributes

Sequence length840 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Deubiquitinating enzyme involved in beta-2 adrenergic receptor (adrb2) recycling. Acts as a regulator of G-protein coupled receptor (GPCR) signaling by mediating the deubiquitination beta-2 adrenergic receptor (adrb2). Plays a central role in adrb2 recycling and resensitization after prolonged agonist stimulation by constitutively binding adrb2, mediating deubiquitination of adrb2 and inhibiting lysosomal trafficking of adrb2. Mediates deubiquitination of both 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains By similarity.

Catalytic activity

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Subcellular location

Cytoplasmperinuclear region By similarity. Cytoplasmcytoskeletonmicrotubule organizing centercentrosome By similarity.

Domain

The UBP-type zinc finger binds 3 zinc ions. However, it does not bind ubiquitin, probably because the conserved Arg in position 55 is replaced by a Glu residue By similarity.

Sequence similarities

Belongs to the peptidase C19 family. USP20/USP33 subfamily.

Contains 2 DUSP domains.

Contains 1 UBP-type zinc finger.

Contains 1 USP domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 840840Ubiquitin carboxyl-terminal hydrolase 20
PRO_0000390421

Regions

Domain101 – 611511USP
Domain613 – 70694DUSP 1
Domain715 – 818104DUSP 2
Zinc finger28 – 9265UBP-type

Sites

Active site1101Nucleophile By similarity
Active site5691Proton acceptor By similarity

Sequences

Sequence LengthMass (Da)Tools
B1WBD7 [UniParc].

Last modified May 20, 2008. Version 1.
Checksum: C75C9C8E2A85C350

FASTA84094,092
        10         20         30         40         50         60 
MADAEDFCPH LDSIGEVTKE DLILKSKGTC ESCGVGGPNL WACLQDGCQS VGCGESYADH 

        70         80         90        100        110        120 
STLHAQDFPS PAHPLKSVPI AVGDDGESES DEDDIKPRGL TGMKNIGNSC YMNAALQALS 

       130        140        150        160        170        180 
NCPPLTQFFL ECGGLVRTDK KPALCKSYQK LVSELWHKKR PSYVVPSSLY HGIKLINPLF 

       190        200        210        220        230        240 
RGYSQQDTQE FLRCLMDQLH EELKEPILPE NQEQEEEERD DQREGERGGT TEEDFLSCDS 

       250        260        270        280        290        300 
GGEMGDGEGG GGVGTLSEME LLIREEVGRG LSEKEKLKER KLSYCHRRTS SEQADEDADV 

       310        320        330        340        350        360 
DTAMIPEPDN DAYMHCSSRS CSPHPVESIS KHSSTPPRSS PLRTAHSYVL KKAQVLSGGK 

       370        380        390        400        410        420 
KRSEVRYRSV ISDIFDGSIL SLVQCLTCDR VSTTIETFQD LSLPIPGKED LAKLHSTIHQ 

       430        440        450        460        470        480 
STVIKAGTCG DSYAAQGWLA FVMDYIRRFV VSCIPSWFWG PMITLEDCLA AFFAADELKG 

       490        500        510        520        530        540 
DNMYSCERCK KLRNGVKYCK VLRLPEVLCI HLKRFRHEVM YSFKIGSHVS FPLEGLNLRP 

       550        560        570        580        590        600 
FLAKECVSRI TTYDLLAVIC HHGSASSGHY ISYCQNVING QWYEFDDQYV TEVHETVVQN 

       610        620        630        640        650        660 
AEAYVLFYRK SSEEAERERQ KVVSLAAMKE SGLLQFYISR EWLNKFNTFA EPGPISNQSF 

       670        680        690        700        710        720 
LCAHGGIPPN KYHYIDDLVV ILPQSVWEYL YNRFGGGPAV NHLYVCSICQ VEIEALAKRR 

       730        740        750        760        770        780 
KTEIDTFIKL NKAFQAEEAP SVIYCISMQW FREWEAFVKA KDSDPPGPID NSKVALTKSS 

       790        800        810        820        830        840 
GHVQLKQGAD YGQISEETWN YLLNIYGGGP EIAIRQTVAQ YQDPEHLHGE QKIEAETRAG 

« Hide

References

[1]NIH - Xenopus Gene Collection (XGC) project
Submitted (APR-2008) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Ovary.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BC161716 mRNA. Translation: AAI61716.1.
RefSeqNP_001121345.1. NM_001127873.1.
UniGeneXl.16107.

3D structure databases

ProteinModelPortalB1WBD7.
SMRB1WBD7. Positions 6-67.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID100158436.
KEGGxla:100158436.

Organism-specific databases

CTD10868.
XenbaseXB-GENE-966533. usp20.

Phylogenomic databases

HOVERGENHBG054196.
KOK11848.

Family and domain databases

Gene3D3.30.2230.10. 2 hits.
3.30.40.10. 1 hit.
InterProIPR006615. Pept_C19_DUSP.
IPR018200. Pept_C19ubi-hydrolase_C_CS.
IPR001394. Peptidase_C19_UCH.
IPR028889. UCH/PAN2.
IPR013083. Znf_RING/FYVE/PHD.
IPR001607. Znf_UBP.
[Graphical view]
PfamPF06337. DUSP. 2 hits.
PF00443. UCH. 1 hit.
PF02148. zf-UBP. 1 hit.
[Graphical view]
SMARTSM00695. DUSP. 2 hits.
[Graphical view]
SUPFAMSSF143791. SSF143791. 2 hits.
PROSITEPS51283. DUSP. 2 hits.
PS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
PS50271. ZF_UBP. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameUBP20_XENLA
AccessionPrimary (citable) accession number: B1WBD7
Entry history
Integrated into UniProtKB/Swiss-Prot: December 15, 2009
Last sequence update: May 20, 2008
Last modified: April 16, 2014
This is version 37 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Peptidase families

Classification of peptidase families and list of entries