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B1WAZ6

- PA24A_XENTR

UniProt

B1WAZ6 - PA24A_XENTR

Protein

Cytosolic phospholipase A2

Gene

pla2g4a

Organism
Xenopus tropicalis (Western clawed frog) (Silurana tropicalis)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 36 (01 Oct 2014)
      Sequence version 1 (20 May 2008)
      Previous versions | rss
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    Functioni

    Selectively hydrolyzes arachidonyl phospholipids in the sn-2 position releasing arachidonic acid. Together with its lysophospholipid activity, it is implicated in the initiation of the inflammatory response By similarity.By similarity

    Catalytic activityi

    Phosphatidylcholine + H2O = 1-acylglycerophosphocholine + a carboxylate.
    2-lysophosphatidylcholine + H2O = glycerophosphocholine + a carboxylate.

    Enzyme regulationi

    Stimulated by agonists such as ATP, EGF, thrombin and bradykinin as well as by cytosolic Ca2+.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi40 – 401Calcium 1By similarity
    Metal bindingi40 – 401Calcium 2By similarity
    Metal bindingi41 – 411Calcium 1; via carbonyl oxygenBy similarity
    Metal bindingi43 – 431Calcium 1By similarity
    Metal bindingi43 – 431Calcium 2By similarity
    Metal bindingi65 – 651Calcium 1By similarity
    Metal bindingi93 – 931Calcium 2By similarity
    Metal bindingi94 – 941Calcium 2; via carbonyl oxygenBy similarity
    Metal bindingi95 – 951Calcium 2By similarity
    Active sitei229 – 2291NucleophileBy similarity
    Active sitei549 – 5491Proton acceptorBy similarity

    GO - Molecular functioni

    1. lysophospholipase activity Source: UniProtKB-EC
    2. metal ion binding Source: UniProtKB-KW
    3. phospholipase A2 activity Source: UniProtKB-EC

    GO - Biological processi

    1. phospholipid catabolic process Source: InterPro

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Biological processi

    Lipid degradation, Lipid metabolism

    Keywords - Ligandi

    Calcium, Metal-binding

    Enzyme and pathway databases

    ReactomeiREACT_195675. Synthesis of PA.
    REACT_195855. Arachidonic acid metabolism.
    REACT_209771. ADP signalling through P2Y purinoceptor 1.
    REACT_213252. Acyl chain remodelling of PE.
    REACT_217968. Acyl chain remodelling of PG.
    REACT_220193. Acyl chain remodelling of PI.
    REACT_221262. Acyl chain remodelling of PC.
    REACT_224622. Acyl chain remodelling of PS.
    REACT_225195. Platelet sensitization by LDL.
    REACT_225940. Acyl chain remodeling of CL.
    REACT_226278. Hydrolysis of LPC.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Cytosolic phospholipase A2
    Short name:
    cPLA2
    Alternative name(s):
    Phospholipase A2 group IVA
    Including the following 2 domains:
    Phospholipase A2 (EC:3.1.1.4)
    Alternative name(s):
    Phosphatidylcholine 2-acylhydrolase
    Lysophospholipase (EC:3.1.1.5)
    Gene namesi
    Name:pla2g4a
    Synonyms:cpla2, pla2g4
    OrganismiXenopus tropicalis (Western clawed frog) (Silurana tropicalis)
    Taxonomic identifieri8364 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraPipoideaPipidaeXenopodinaeXenopusSilurana
    ProteomesiUP000008143: Unplaced

    Organism-specific databases

    XenbaseiXB-GENE-5838849. pla2g4a.

    Subcellular locationi

    Cytoplasm By similarity. Cytoplasmic vesicle By similarity
    Note: Translocates to membrane vesicles in a calcium-dependent fashion.By similarity

    GO - Cellular componenti

    1. cytoplasmic membrane-bounded vesicle Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm, Cytoplasmic vesicle

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 749749Cytosolic phospholipase A2PRO_0000345137Add
    BLAST

    Expressioni

    Gene expression databases

    BgeeiB1WAZ6.

    Interactioni

    Protein-protein interaction databases

    STRINGi8364.ENSXETP00000037820.

    Structurei

    3D structure databases

    ProteinModelPortaliB1WAZ6.
    SMRiB1WAZ6. Positions 13-721.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini5 – 106102C2PROSITE-ProRule annotationAdd
    BLAST
    Domaini138 – 740603PLA2cPROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 178178Phospholipid bindingCuratedAdd
    BLAST

    Domaini

    The N-terminal C2 domain associates with lipid membranes upon calcium binding. It modulates enzyme activity by presenting the active site to its substrate in response to elevations of cytosolic Ca2+ By similarity.By similarity

    Sequence similaritiesi

    Contains 1 C2 domain.PROSITE-ProRule annotation
    Contains 1 PLA2c domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiNOG257248.
    HOGENOMiHOG000115420.
    HOVERGENiHBG053479.
    KOiK16342.

    Family and domain databases

    Gene3Di2.60.40.150. 1 hit.
    InterProiIPR016035. Acyl_Trfase/lysoPLipase.
    IPR000008. C2_dom.
    IPR002642. LysoPLipase_cat_dom.
    [Graphical view]
    PfamiPF00168. C2. 1 hit.
    PF01735. PLA2_B. 1 hit.
    [Graphical view]
    SMARTiSM00239. C2. 1 hit.
    SM00022. PLAc. 1 hit.
    [Graphical view]
    SUPFAMiSSF49562. SSF49562. 1 hit.
    SSF52151. SSF52151. 1 hit.
    PROSITEiPS50004. C2. 1 hit.
    PS51210. PLA2C. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    B1WAZ6-1 [UniParc]FASTAAdd to Basket

    « Hide

    MASIDPYQHI IVEHQYSHRF TVTVIKATNV TKGTFGDMLD TPDPYVELYI    50
    SSAPDSRKRT KHFNNNINPV WNETFEFILD PNQDNVLEIT LMDANYVMDE 100
    SLGTTTFPIS SVKPGEKKQV PFTFNKVTEM ILEFLLEVCS STDLRFSMAL 150
    CDQEKFFRQK RKNQVINGLR KLLGPEKTKD LNPTSRDVPV IAVLGSGGGF 200
    RAMIGFSGVM KALFESGVLD CVTYIAGLSG STWYMSALYS HADFPNKGPK 250
    EINKELMNNV SHNPLLLLTP QKVKRYIEAL WKKKSSGQPV TFTDIFAMLI 300
    GETLIKDRMN RKLSHMQEKI SHGQCPLPLF TCLHVKPDVS ELMFADWVEF 350
    SPYEIGMAKY GTFMPPDHFG SKFFMGTVIK KYEENPLHFL MGVWGSAFSI 400
    LINRVLGVST NNQGSTMEEE IENLKPKHIL GNDSSDSDDE MQEPKGTENS 450
    KAEEEYQRNN QASWVQRMLM ALLGDSALFN TREGRAGKVH NFMLGLNLNT 500
    SYPYSPLSGL CTQQSMEEDE FDAAVADPDE FEQIYEPLDV KSKKIHIVDS 550
    GLTFNLPYPL ILRPQRGVDL IISFDFSARP SDSSPPFKEL LLAEKWARMN 600
    KLPFPKIDPH VFDREGLKEC YIFKPKNPSV EKDCPTVIHF VLANLQFRNF 650
    KAPGVPRETA EEKEFADFDI FDDPETPFST FNFQYPNEAF KRLHDLMEFN 700
    TLNNINVIKQ AMVESIEYRK QHPSRCSVSL NDVEARKLLH KDSQSKFQM 749
    Length:749
    Mass (Da):85,413
    Last modified:May 20, 2008 - v1
    Checksum:i18EC0E15ED902CAE
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC161557 mRNA. Translation: AAI61557.1.
    RefSeqiNP_001120577.1. NM_001127105.1.
    UniGeneiStr.52630.

    Genome annotation databases

    GeneIDi100145731.
    KEGGixtr:100145731.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC161557 mRNA. Translation: AAI61557.1 .
    RefSeqi NP_001120577.1. NM_001127105.1.
    UniGenei Str.52630.

    3D structure databases

    ProteinModelPortali B1WAZ6.
    SMRi B1WAZ6. Positions 13-721.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 8364.ENSXETP00000037820.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 100145731.
    KEGGi xtr:100145731.

    Organism-specific databases

    CTDi 5321.
    Xenbasei XB-GENE-5838849. pla2g4a.

    Phylogenomic databases

    eggNOGi NOG257248.
    HOGENOMi HOG000115420.
    HOVERGENi HBG053479.
    KOi K16342.

    Enzyme and pathway databases

    Reactomei REACT_195675. Synthesis of PA.
    REACT_195855. Arachidonic acid metabolism.
    REACT_209771. ADP signalling through P2Y purinoceptor 1.
    REACT_213252. Acyl chain remodelling of PE.
    REACT_217968. Acyl chain remodelling of PG.
    REACT_220193. Acyl chain remodelling of PI.
    REACT_221262. Acyl chain remodelling of PC.
    REACT_224622. Acyl chain remodelling of PS.
    REACT_225195. Platelet sensitization by LDL.
    REACT_225940. Acyl chain remodeling of CL.
    REACT_226278. Hydrolysis of LPC.

    Gene expression databases

    Bgeei B1WAZ6.

    Family and domain databases

    Gene3Di 2.60.40.150. 1 hit.
    InterProi IPR016035. Acyl_Trfase/lysoPLipase.
    IPR000008. C2_dom.
    IPR002642. LysoPLipase_cat_dom.
    [Graphical view ]
    Pfami PF00168. C2. 1 hit.
    PF01735. PLA2_B. 1 hit.
    [Graphical view ]
    SMARTi SM00239. C2. 1 hit.
    SM00022. PLAc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF49562. SSF49562. 1 hit.
    SSF52151. SSF52151. 1 hit.
    PROSITEi PS50004. C2. 1 hit.
    PS51210. PLA2C. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. NIH - Xenopus Gene Collection (XGC) project
      Submitted (APR-2008) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: N6.
      Tissue: Intestine.

    Entry informationi

    Entry nameiPA24A_XENTR
    AccessioniPrimary (citable) accession number: B1WAZ6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 22, 2008
    Last sequence update: May 20, 2008
    Last modified: October 1, 2014
    This is version 36 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3