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Protein

Cytosolic phospholipase A2

Gene

pla2g4a

Organism
Xenopus tropicalis (Western clawed frog) (Silurana tropicalis)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Selectively hydrolyzes arachidonyl phospholipids in the sn-2 position releasing arachidonic acid. Together with its lysophospholipid activity, it is implicated in the initiation of the inflammatory response (By similarity).By similarity

Catalytic activityi

Phosphatidylcholine + H2O = 1-acylglycerophosphocholine + a carboxylate.
2-lysophosphatidylcholine + H2O = glycerophosphocholine + a carboxylate.

Enzyme regulationi

Stimulated by agonists such as ATP, EGF, thrombin and bradykinin as well as by cytosolic Ca2+.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi40 – 401Calcium 1By similarity
Metal bindingi40 – 401Calcium 2By similarity
Metal bindingi41 – 411Calcium 1; via carbonyl oxygenBy similarity
Metal bindingi43 – 431Calcium 1By similarity
Metal bindingi43 – 431Calcium 2By similarity
Metal bindingi65 – 651Calcium 1By similarity
Metal bindingi93 – 931Calcium 2By similarity
Metal bindingi94 – 941Calcium 2; via carbonyl oxygenBy similarity
Metal bindingi95 – 951Calcium 2By similarity
Active sitei229 – 2291NucleophileBy similarity
Active sitei549 – 5491Proton acceptorBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Lipid degradation, Lipid metabolism

Keywords - Ligandi

Calcium, Metal-binding

Enzyme and pathway databases

ReactomeiREACT_277072. Hydrolysis of LPC.
REACT_291498. Acyl chain remodelling of PG.
REACT_294650. Platelet sensitization by LDL.
REACT_303938. Acyl chain remodeling of CL.
REACT_310404. Acyl chain remodelling of PC.
REACT_311901. Acyl chain remodelling of PE.
REACT_312268. Acyl chain remodelling of PS.
REACT_320378. Synthesis of PA.
REACT_320824. Acyl chain remodelling of PI.
REACT_321625. phospho-PLA2 pathway.
REACT_322375. ADP signalling through P2Y purinoceptor 1.
REACT_344119. Arachidonic acid metabolism.

Names & Taxonomyi

Protein namesi
Recommended name:
Cytosolic phospholipase A2
Short name:
cPLA2
Alternative name(s):
Phospholipase A2 group IVA
Including the following 2 domains:
Phospholipase A2 (EC:3.1.1.4)
Alternative name(s):
Phosphatidylcholine 2-acylhydrolase
Lysophospholipase (EC:3.1.1.5)
Gene namesi
Name:pla2g4a
Synonyms:cpla2, pla2g4
OrganismiXenopus tropicalis (Western clawed frog) (Silurana tropicalis)
Taxonomic identifieri8364 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraPipoideaPipidaeXenopodinaeXenopusSilurana
ProteomesiUP000008143 Componenti: Unassembled WGS sequence

Organism-specific databases

XenbaseiXB-GENE-5838849. pla2g4a.

Subcellular locationi

  • Cytoplasm By similarity
  • Cytoplasmic vesicle By similarity

  • Note: Translocates to membrane vesicles in a calcium-dependent fashion.By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoplasmic vesicle

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 749749Cytosolic phospholipase A2PRO_0000345137Add
BLAST

Expressioni

Gene expression databases

BgeeiB1WAZ6.
ExpressionAtlasiB1WAZ6. baseline.

Interactioni

Protein-protein interaction databases

STRINGi8364.ENSXETP00000037820.

Structurei

3D structure databases

ProteinModelPortaliB1WAZ6.
SMRiB1WAZ6. Positions 13-721.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini5 – 106102C2PROSITE-ProRule annotationAdd
BLAST
Domaini138 – 740603PLA2cPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 178178Phospholipid bindingCuratedAdd
BLAST

Domaini

The N-terminal C2 domain associates with lipid membranes upon calcium binding. It modulates enzyme activity by presenting the active site to its substrate in response to elevations of cytosolic Ca2+ (By similarity).By similarity

Sequence similaritiesi

Contains 1 C2 domain.PROSITE-ProRule annotation
Contains 1 PLA2c domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiNOG257248.
HOGENOMiHOG000115420.
HOVERGENiHBG053479.
InParanoidiB1WAZ6.
KOiK16342.

Family and domain databases

Gene3Di2.60.40.150. 1 hit.
InterProiIPR016035. Acyl_Trfase/lysoPLipase.
IPR000008. C2_dom.
IPR002642. LysoPLipase_cat_dom.
[Graphical view]
PfamiPF00168. C2. 1 hit.
PF01735. PLA2_B. 1 hit.
[Graphical view]
SMARTiSM00239. C2. 1 hit.
SM00022. PLAc. 1 hit.
[Graphical view]
SUPFAMiSSF49562. SSF49562. 1 hit.
SSF52151. SSF52151. 1 hit.
PROSITEiPS50004. C2. 1 hit.
PS51210. PLA2C. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B1WAZ6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASIDPYQHI IVEHQYSHRF TVTVIKATNV TKGTFGDMLD TPDPYVELYI
60 70 80 90 100
SSAPDSRKRT KHFNNNINPV WNETFEFILD PNQDNVLEIT LMDANYVMDE
110 120 130 140 150
SLGTTTFPIS SVKPGEKKQV PFTFNKVTEM ILEFLLEVCS STDLRFSMAL
160 170 180 190 200
CDQEKFFRQK RKNQVINGLR KLLGPEKTKD LNPTSRDVPV IAVLGSGGGF
210 220 230 240 250
RAMIGFSGVM KALFESGVLD CVTYIAGLSG STWYMSALYS HADFPNKGPK
260 270 280 290 300
EINKELMNNV SHNPLLLLTP QKVKRYIEAL WKKKSSGQPV TFTDIFAMLI
310 320 330 340 350
GETLIKDRMN RKLSHMQEKI SHGQCPLPLF TCLHVKPDVS ELMFADWVEF
360 370 380 390 400
SPYEIGMAKY GTFMPPDHFG SKFFMGTVIK KYEENPLHFL MGVWGSAFSI
410 420 430 440 450
LINRVLGVST NNQGSTMEEE IENLKPKHIL GNDSSDSDDE MQEPKGTENS
460 470 480 490 500
KAEEEYQRNN QASWVQRMLM ALLGDSALFN TREGRAGKVH NFMLGLNLNT
510 520 530 540 550
SYPYSPLSGL CTQQSMEEDE FDAAVADPDE FEQIYEPLDV KSKKIHIVDS
560 570 580 590 600
GLTFNLPYPL ILRPQRGVDL IISFDFSARP SDSSPPFKEL LLAEKWARMN
610 620 630 640 650
KLPFPKIDPH VFDREGLKEC YIFKPKNPSV EKDCPTVIHF VLANLQFRNF
660 670 680 690 700
KAPGVPRETA EEKEFADFDI FDDPETPFST FNFQYPNEAF KRLHDLMEFN
710 720 730 740
TLNNINVIKQ AMVESIEYRK QHPSRCSVSL NDVEARKLLH KDSQSKFQM
Length:749
Mass (Da):85,413
Last modified:May 20, 2008 - v1
Checksum:i18EC0E15ED902CAE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC161557 mRNA. Translation: AAI61557.1.
RefSeqiNP_001120577.1. NM_001127105.1.
UniGeneiStr.52630.

Genome annotation databases

GeneIDi100145731.
KEGGixtr:100145731.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC161557 mRNA. Translation: AAI61557.1.
RefSeqiNP_001120577.1. NM_001127105.1.
UniGeneiStr.52630.

3D structure databases

ProteinModelPortaliB1WAZ6.
SMRiB1WAZ6. Positions 13-721.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi8364.ENSXETP00000037820.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi100145731.
KEGGixtr:100145731.

Organism-specific databases

CTDi5321.
XenbaseiXB-GENE-5838849. pla2g4a.

Phylogenomic databases

eggNOGiNOG257248.
HOGENOMiHOG000115420.
HOVERGENiHBG053479.
InParanoidiB1WAZ6.
KOiK16342.

Enzyme and pathway databases

ReactomeiREACT_277072. Hydrolysis of LPC.
REACT_291498. Acyl chain remodelling of PG.
REACT_294650. Platelet sensitization by LDL.
REACT_303938. Acyl chain remodeling of CL.
REACT_310404. Acyl chain remodelling of PC.
REACT_311901. Acyl chain remodelling of PE.
REACT_312268. Acyl chain remodelling of PS.
REACT_320378. Synthesis of PA.
REACT_320824. Acyl chain remodelling of PI.
REACT_321625. phospho-PLA2 pathway.
REACT_322375. ADP signalling through P2Y purinoceptor 1.
REACT_344119. Arachidonic acid metabolism.

Gene expression databases

BgeeiB1WAZ6.
ExpressionAtlasiB1WAZ6. baseline.

Family and domain databases

Gene3Di2.60.40.150. 1 hit.
InterProiIPR016035. Acyl_Trfase/lysoPLipase.
IPR000008. C2_dom.
IPR002642. LysoPLipase_cat_dom.
[Graphical view]
PfamiPF00168. C2. 1 hit.
PF01735. PLA2_B. 1 hit.
[Graphical view]
SMARTiSM00239. C2. 1 hit.
SM00022. PLAc. 1 hit.
[Graphical view]
SUPFAMiSSF49562. SSF49562. 1 hit.
SSF52151. SSF52151. 1 hit.
PROSITEiPS50004. C2. 1 hit.
PS51210. PLA2C. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. NIH - Xenopus Gene Collection (XGC) project
    Submitted (APR-2008) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: N6.
    Tissue: Intestine.

Entry informationi

Entry nameiPA24A_XENTR
AccessioniPrimary (citable) accession number: B1WAZ6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 22, 2008
Last sequence update: May 20, 2008
Last modified: May 27, 2015
This is version 41 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.