Reviewed, UniProtKB/Swiss-Prot B1WAZ6 (PA24A_XENTR)

Names and origin

Protein names

Recommended name:
    Cytosolic phospholipase A2
      Short name=cPLA2
Alternative name(s):
    Phospholipase A2 group IVA
Including the following 2 domains:
    1- Recommended name:
            Phospholipase A2
              EC=3.1.1.4
        Alternative name(s):
            Phosphatidylcholine 2-acylhydrolase
    2- Recommended name:
            Lysophospholipase
              EC=3.1.1.5

Gene names

Name:	pla2g4a
Synonyms:	cpla2, pla2g4

Organism

Xenopus tropicalis (Western clawed frog) (Silurana tropicalis)

Taxonomic identifier

8364 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Amphibia › Batrachia › Anura › Mesobatrachia › Pipoidea › Pipidae › Xenopodinae › Xenopus › Silurana

Protein attributes

Sequence length	749 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Selectively hydrolyzes arachidonyl phospholipids in the sn-2 position releasing arachidonic acid. Together with its lysophospholipid activity, it is implicated in the initiation of the inflammatory response By similarity.
Catalytic activity	Phosphatidylcholine + H2O = 1-acylglycerophosphocholine + a carboxylate. 2-lysophosphatidylcholine + H2O = glycerophosphocholine + a carboxylate.
Enzyme regulation	Stimulated by agonists such as ATP, EGF, thrombin and bradykinin as well as by cytosolic Ca2+ By similarity.
Subcellular location	Cytoplasm By similarity. Cytoplasmic vesicle By similarity. Note: Translocates to membrane vesicles in a calcium-dependent fashion By similarity.
Domain	The N-terminal C2 domain, by its association with lipid membranes, mediates the regulation of CPLA2 by presenting the active site to its substrate in response to elevations of cytosolic Ca2+ By similarity.
Sequence similarities	Contains 1 C2 domain. Contains 1 PLA2c domain.

Ontologies

Keywords
Biological process	Lipid degradation
Cellular component	Cytoplasm Cytoplasmic vesicle
Ligand	Calcium
Molecular function	Hydrolase
Gene Ontology (GO)
Biological process	phospholipid catabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasmic vesicle Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	calcium ion binding Inferred from electronic annotation. Source: UniProtKB-KW lysophospholipase activity Inferred from electronic annotation. Source: EC phospholipase A2 activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 749	749	Cytosolic phospholipase A2		PRO_0000345137
Regions
	Domain	5 – 106	102	C2
	Domain	138 – 740	603	PLA2c
	Region	1 – 178	178	Phospholipid binding Probable

Sequences

	Sequence		Length	Mass (Da)
	B1WAZ6-1 [UniParc]. Last modified May 20, 2008. Version 1. Checksum: 18EC0E15ED902CAE	FASTA	749	85,413
	10 20 30 40 50 60 MASIDPYQHI IVEHQYSHRF TVTVIKATNV TKGTFGDMLD TPDPYVELYI SSAPDSRKRT 70 80 90 100 110 120 KHFNNNINPV WNETFEFILD PNQDNVLEIT LMDANYVMDE SLGTTTFPIS SVKPGEKKQV 130 140 150 160 170 180 PFTFNKVTEM ILEFLLEVCS STDLRFSMAL CDQEKFFRQK RKNQVINGLR KLLGPEKTKD 190 200 210 220 230 240 LNPTSRDVPV IAVLGSGGGF RAMIGFSGVM KALFESGVLD CVTYIAGLSG STWYMSALYS 250 260 270 280 290 300 HADFPNKGPK EINKELMNNV SHNPLLLLTP QKVKRYIEAL WKKKSSGQPV TFTDIFAMLI 310 320 330 340 350 360 GETLIKDRMN RKLSHMQEKI SHGQCPLPLF TCLHVKPDVS ELMFADWVEF SPYEIGMAKY 370 380 390 400 410 420 GTFMPPDHFG SKFFMGTVIK KYEENPLHFL MGVWGSAFSI LINRVLGVST NNQGSTMEEE 430 440 450 460 470 480 IENLKPKHIL GNDSSDSDDE MQEPKGTENS KAEEEYQRNN QASWVQRMLM ALLGDSALFN 490 500 510 520 530 540 TREGRAGKVH NFMLGLNLNT SYPYSPLSGL CTQQSMEEDE FDAAVADPDE FEQIYEPLDV 550 560 570 580 590 600 KSKKIHIVDS GLTFNLPYPL ILRPQRGVDL IISFDFSARP SDSSPPFKEL LLAEKWARMN 610 620 630 640 650 660 KLPFPKIDPH VFDREGLKEC YIFKPKNPSV EKDCPTVIHF VLANLQFRNF KAPGVPRETA 670 680 690 700 710 720 EEKEFADFDI FDDPETPFST FNFQYPNEAF KRLHDLMEFN TLNNINVIKQ AMVESIEYRK 730 740 QHPSRCSVSL NDVEARKLLH KDSQSKFQM

References

[1]	NIH - Xenopus Gene Collection (XGC) project Submitted (APR-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: N6. Tissue: Intestine.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BC161557 mRNA. Translation: AAI61557.1.
RefSeq	NP_001120577.1.
UniGene	Str.52630
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	100145731.
KEGG	xtr:100145731.
Family and domain databases
InterPro	IPR000008. C2_Ca-dep. IPR018029. C2_membr_targeting. IPR002642. PLA2_B. [Graphical view]
Pfam	PF00168. C2. 1 hit. PF01735. PLA2_B. 1 hit. [Graphical view]
SMART	SM00239. C2. 1 hit. SM00022. PLAc. 1 hit. [Graphical view]
PROSITE	PS50004. C2. 1 hit. PS51210. PLA2C. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PA24A_XENTR

Accession

Primary (citable) accession number: B1WAZ6

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 22, 2008
Last sequence update:	May 20, 2008
Last modified:	June 16, 2009
This is version 8 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Xenopus annotation project