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B1W5J2 (DNLI_STRGG) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 45. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Probable DNA ligase

EC=6.5.1.1
Alternative name(s):
Polydeoxyribonucleotide synthase [ATP]
Gene names
Name:lig
Ordered Locus Names:SGR_6320
OrganismStreptomyces griseus subsp. griseus (strain JCM 4626 / NBRC 13350) [Complete proteome] [HAMAP]
Taxonomic identifier455632 [NCBI]
Taxonomic lineageBacteriaActinobacteriaActinobacteridaeActinomycetalesStreptomycineaeStreptomycetaceaeStreptomyces

Protein attributes

Sequence length511 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

DNA ligase that seals nicks in double-stranded DNA during DNA replication, DNA recombination and DNA repair By similarity. HAMAP-Rule MF_00407

Catalytic activity

ATP + (deoxyribonucleotide)(n) + (deoxyribonucleotide)(m) = AMP + diphosphate + (deoxyribonucleotide)(n+m). HAMAP-Rule MF_00407

Cofactor

Divalent metal cations By similarity. HAMAP-Rule MF_00407

Sequence similarities

Belongs to the ATP-dependent DNA ligase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 511511Probable DNA ligase HAMAP-Rule MF_00407
PRO_0000365241

Sites

Active site2101N6-AMP-lysine intermediate By similarity
Binding site2081ATP By similarity
Binding site2151ATP By similarity
Binding site2301ATP By similarity
Binding site2591ATP By similarity
Binding site2991ATP By similarity
Binding site3771ATP By similarity
Binding site3831ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
B1W5J2 [UniParc].

Last modified May 20, 2008. Version 1.
Checksum: 715B6B5D62E79FDB

FASTA51154,767
        10         20         30         40         50         60 
MLLAELAQVS LEVAATSARS RKTALLAGLF RNAGPEDVPV VIPYLAGRLP QGRIGVGWRS 

        70         80         90        100        110        120 
LGEPVEPACD PTLTVTGVDA ELTALAATSG PGSQARRRER LRTLFAAATA DEQRFLRALL 

       130        140        150        160        170        180 
TGEVRQGALD AVAADALAHA AGAPPADVRR AVMLAGSLQD VATVLLADGP GALADFRLTV 

       190        200        210        220        230        240 
GRPVQPMLAR SAASVGEALD KLGPCAVEEK LDGIRVQVHR DGERIRAYTR TLDDITDRLP 

       250        260        270        280        290        300 
ELVSAVAALH ARRFVLDGEL IALGEDGRPR PFQETASRVG SRRDVAGAAA HVPVVPVFFD 

       310        320        330        340        350        360 
ALRVDGEDLL DRPFADRHAA LARLLPDDLR VRRTVVADPD APEARAAADA FLAATLERGH 

       370        380        390        400        410        420 
EGVVVKDLAA AYSAGRRGAS WLKVKPVHTL DLVVLAVERG SGRRTGKLSN LHLGARRPDG 

       430        440        450        460        470        480 
TFAMLGKTFK GLTDALLDWQ TARLRELATD DDGHVVTVRP ELVVEIAYDG LQRSTRYPAG 

       490        500        510 
VTLRFARVLR YREDKTAREA DTVETVLSRQ R 

« Hide

References

[1]"Genome sequence of the streptomycin-producing microorganism Streptomyces griseus IFO 13350."
Ohnishi Y., Ishikawa J., Hara H., Suzuki H., Ikenoya M., Ikeda H., Yamashita A., Hattori M., Horinouchi S.
J. Bacteriol. 190:4050-4060(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: JCM 4626 / NBRC 13350.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AP009493 Genomic DNA. Translation: BAG23149.1.
RefSeqYP_001827832.1. NC_010572.1.

3D structure databases

ProteinModelPortalB1W5J2.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING455632.SGR_6320.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAG23149; BAG23149; SGR_6320.
GeneID6211366.
KEGGsgr:SGR_6320.
PATRIC23759499. VBIStrGri32265_6478.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1793.
HOGENOMHOG000036008.
KOK01971.
OMAGRPRPFQ.
OrthoDBEOG6Q8HXT.
ProtClustDBPRK03180.

Enzyme and pathway databases

BioCycSGRI455632:GD3A-6406-MONOMER.

Family and domain databases

Gene3D1.10.3260.10. 1 hit.
2.40.50.140. 1 hit.
HAMAPMF_00407. DNA_ligase.
InterProIPR022865. DNA_ligae_ATP-dep_bac/arc.
IPR000977. DNA_ligase_ATP-dep.
IPR012309. DNA_ligase_ATP-dep_C.
IPR012310. DNA_ligase_ATP-dep_cent.
IPR016059. DNA_ligase_ATP-dep_CS.
IPR012308. DNA_ligase_ATP-dep_N.
IPR012340. NA-bd_OB-fold.
[Graphical view]
PfamPF04679. DNA_ligase_A_C. 1 hit.
PF01068. DNA_ligase_A_M. 1 hit.
PF04675. DNA_ligase_A_N. 2 hits.
[Graphical view]
SUPFAMSSF117018. SSF117018. 1 hit.
SSF50249. SSF50249. 1 hit.
TIGRFAMsTIGR00574. dnl1. 1 hit.
PROSITEPS00697. DNA_LIGASE_A1. 1 hit.
PS50160. DNA_LIGASE_A3. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDNLI_STRGG
AccessionPrimary (citable) accession number: B1W5J2
Entry history
Integrated into UniProtKB/Swiss-Prot: March 3, 2009
Last sequence update: May 20, 2008
Last modified: February 19, 2014
This is version 45 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families