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B1W5F4

- BIOB_STRGG

UniProt

B1W5F4 - BIOB_STRGG

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Protein

Biotin synthase

Gene

bioB

Organism
Streptomyces griseus subsp. griseus (strain JCM 4626 / NBRC 13350)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism.UniRule annotation

Catalytic activityi

Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

Cofactori

Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation
Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi65 – 651Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation
Metal bindingi69 – 691Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation
Metal bindingi72 – 721Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation
Metal bindingi109 – 1091Iron-sulfur 2 (2Fe-2S)UniRule annotation
Metal bindingi142 – 1421Iron-sulfur 2 (2Fe-2S)UniRule annotation
Metal bindingi202 – 2021Iron-sulfur 2 (2Fe-2S)UniRule annotation
Metal bindingi272 – 2721Iron-sulfur 2 (2Fe-2S)UniRule annotation

GO - Molecular functioni

  1. 2 iron, 2 sulfur cluster binding Source: UniProtKB-KW
  2. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
  3. biotin synthase activity Source: UniProtKB-HAMAP
  4. iron ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. biotin biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Biotin biosynthesis

Keywords - Ligandi

2Fe-2S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciSGRI455632:GD3A-6368-MONOMER.
UniPathwayiUPA00078; UER00162.

Names & Taxonomyi

Protein namesi
Recommended name:
Biotin synthaseUniRule annotation (EC:2.8.1.6UniRule annotation)
Gene namesi
Name:bioBUniRule annotation
Ordered Locus Names:SGR_6282
OrganismiStreptomyces griseus subsp. griseus (strain JCM 4626 / NBRC 13350)
Taxonomic identifieri455632 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesStreptomycineaeStreptomycetaceaeStreptomyces
ProteomesiUP000001685: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 427427Biotin synthasePRO_0000381665Add
BLAST

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi455632.SGR_6282.

Structurei

3D structure databases

ProteinModelPortaliB1W5F4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the radical SAM superfamily. Biotin synthase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0502.
HOGENOMiHOG000239958.
KOiK01012.
OMAiTSEATYG.
OrthoDBiEOG622PMP.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_01694. BioB.
InterProiIPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view]
PfamiPF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view]
SMARTiSM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00433. bioB. 1 hit.

Sequencei

Sequence statusi: Complete.

B1W5F4-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MDLLNTLVDK GLRREPPTRE EALAVLATSD DELLDVVAAA GKVRRQWFGR
60 70 80 90 100
RVKLNYLVNL KSGLCPEDCS YCSQRLGSKA GILKYTWLKP EEASRAAAAG
110 120 130 140 150
VAGGAKRVCL VASGRGPTDR DVDRVSKTIE AIKEENEGIE VCACLGLLSD
160 170 180 190 200
GQADRLRSAG ADAYNHNLNT SEETYGAITT THTYADRVET VQQAQAAGLS
210 220 230 240 250
ACSGLIAGMG ESDKDLVDVV FALRDLDPDS VPVNFLIPFE GTPLAKEWNL
260 270 280 290 300
TPQRCLRILA MVRFVCPDVE VRLAGGREVH LRSMQPLALH LVNSIFLGDY
310 320 330 340 350
LTSEGQAGQT DLDMIADAGF EVEGAGTTTL PRHRADALSG GCGTRDATAG
360 370 380 390 400
SGCGSHEDSG AGGCGSHGGG AGCGPCGGHG EREPVAVADS AAGESVPGAR
410 420
TETSGAARTD LVAVRRRGAG TDLAPNA
Length:427
Mass (Da):44,605
Last modified:May 20, 2008 - v1
Checksum:i3D488E21BE1E15BC
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AP009493 Genomic DNA. Translation: BAG23111.1.
RefSeqiYP_001827794.1. NC_010572.1.

Genome annotation databases

EnsemblBacteriaiBAG23111; BAG23111; SGR_6282.
GeneIDi6211340.
KEGGisgr:SGR_6282.
PATRICi23759423. VBIStrGri32265_6440.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AP009493 Genomic DNA. Translation: BAG23111.1 .
RefSeqi YP_001827794.1. NC_010572.1.

3D structure databases

ProteinModelPortali B1W5F4.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 455632.SGR_6282.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai BAG23111 ; BAG23111 ; SGR_6282 .
GeneIDi 6211340.
KEGGi sgr:SGR_6282.
PATRICi 23759423. VBIStrGri32265_6440.

Phylogenomic databases

eggNOGi COG0502.
HOGENOMi HOG000239958.
KOi K01012.
OMAi TSEATYG.
OrthoDBi EOG622PMP.

Enzyme and pathway databases

UniPathwayi UPA00078 ; UER00162 .
BioCyci SGRI455632:GD3A-6368-MONOMER.

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
HAMAPi MF_01694. BioB.
InterProi IPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view ]
Pfami PF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view ]
SMARTi SM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00433. bioB. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Genome sequence of the streptomycin-producing microorganism Streptomyces griseus IFO 13350."
    Ohnishi Y., Ishikawa J., Hara H., Suzuki H., Ikenoya M., Ikeda H., Yamashita A., Hattori M., Horinouchi S.
    J. Bacteriol. 190:4050-4060(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: JCM 4626 / NBRC 13350.

Entry informationi

Entry nameiBIOB_STRGG
AccessioniPrimary (citable) accession number: B1W5F4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: May 20, 2008
Last modified: October 1, 2014
This is version 45 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3