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B1W3G2 (RUVC_STRGG) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 41. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Crossover junction endodeoxyribonuclease RuvC

EC=3.1.22.4
Alternative name(s):
Holliday junction nuclease RuvC
Holliday junction resolvase RuvC
Gene names
Name:ruvC
Ordered Locus Names:SGR_6015
OrganismStreptomyces griseus subsp. griseus (strain JCM 4626 / NBRC 13350) [Complete proteome] [HAMAP]
Taxonomic identifier455632 [NCBI]
Taxonomic lineageBacteriaActinobacteriaActinobacteridaeActinomycetalesStreptomycineaeStreptomycetaceaeStreptomyces

Protein attributes

Sequence length204 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Nuclease that resolves Holliday junction intermediates in genetic recombination. Cleaves the cruciform structure in supercoiled DNA by nicking to strands with the same polarity at sites symmetrically opposed at the junction in the homologous arms and leaves a 5'-terminal phosphate and a 3'-terminal hydroxyl group By similarity. HAMAP-Rule MF_00034

Catalytic activity

Endonucleolytic cleavage at a junction such as a reciprocal single-stranded crossover between two homologous DNA duplexes (Holliday junction). HAMAP-Rule MF_00034

Cofactor

Binds 1 magnesium ion per subunit By similarity. HAMAP-Rule MF_00034

Sequence similarities

Belongs to the RuvC family.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 204204Crossover junction endodeoxyribonuclease RuvC HAMAP-Rule MF_00034
PRO_1000090565

Sites

Metal binding71Magnesium By similarity
Metal binding681Magnesium By similarity
Metal binding1411Magnesium By similarity
Metal binding1441Magnesium By similarity

Sequences

Sequence LengthMass (Da)Tools
B1W3G2 [UniParc].

Last modified May 20, 2008. Version 1.
Checksum: 87227CCDF404114A

FASTA20420,971
        10         20         30         40         50         60 
MRVLGIDPGL TRCGVGVVEG VAGRPLTMIG VGVVRTSSDA ELGDRLVAVE RGIEQWLDEH 

        70         80         90        100        110        120 
SPGYVAVERV FAQHNVRTVM GTAQASAVAM LCAARRGIPV ALHTPSEVKA AVTGSGRADK 

       130        140        150        160        170        180 
AQVGAMVTRL LRLDAPPKPA DAADALALAI CHIWRAPAQN RLQQAVAAHR TSGASRTPGA 

       190        200 
AGTPGPSRTP GAPGTSRTLK GRTA 

« Hide

References

[1]"Genome sequence of the streptomycin-producing microorganism Streptomyces griseus IFO 13350."
Ohnishi Y., Ishikawa J., Hara H., Suzuki H., Ikenoya M., Ikeda H., Yamashita A., Hattori M., Horinouchi S.
J. Bacteriol. 190:4050-4060(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: JCM 4626 / NBRC 13350.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AP009493 Genomic DNA. Translation: BAG22844.1.
RefSeqYP_001827527.1. NC_010572.1.

3D structure databases

ProteinModelPortalB1W3G2.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING455632.SGR_6015.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAG22844; BAG22844; SGR_6015.
GeneID6211160.
KEGGsgr:SGR_6015.
PATRIC23758869. VBIStrGri32265_6166.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0817.
HOGENOMHOG000012182.
KOK01159.
OMAYTALQMK.
OrthoDBEOG6RG044.
ProtClustDBPRK00039.

Enzyme and pathway databases

BioCycSGRI455632:GD3A-6098-MONOMER.

Family and domain databases

Gene3D3.30.420.10. 1 hit.
HAMAPMF_00034. RuvC.
InterProIPR012337. RNaseH-like_dom.
IPR020563. X-over_junc_endoDNase_Mg_BS.
IPR002176. X-over_junc_endoDNase_RuvC.
[Graphical view]
PfamPF02075. RuvC. 1 hit.
[Graphical view]
PRINTSPR00696. RSOLVASERUVC.
SUPFAMSSF53098. SSF53098. 1 hit.
TIGRFAMsTIGR00228. ruvC. 1 hit.
PROSITEPS01321. RUVC. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameRUVC_STRGG
AccessionPrimary (citable) accession number: B1W3G2
Entry history
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: May 20, 2008
Last modified: April 16, 2014
This is version 41 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families