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B1W3G2

- RUVC_STRGG

UniProt

B1W3G2 - RUVC_STRGG

Protein

Crossover junction endodeoxyribonuclease RuvC

Gene

ruvC

Organism
Streptomyces griseus subsp. griseus (strain JCM 4626 / NBRC 13350)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 43 (01 Oct 2014)
      Sequence version 1 (20 May 2008)
      Previous versions | rss
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    Functioni

    Nuclease that resolves Holliday junction intermediates in genetic recombination. Cleaves the cruciform structure in supercoiled DNA by nicking to strands with the same polarity at sites symmetrically opposed at the junction in the homologous arms and leaves a 5'-terminal phosphate and a 3'-terminal hydroxyl group.UniRule annotation

    Catalytic activityi

    Endonucleolytic cleavage at a junction such as a reciprocal single-stranded crossover between two homologous DNA duplexes (Holliday junction).UniRule annotation

    Cofactori

    Binds 1 magnesium ion per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi7 – 71MagnesiumUniRule annotation
    Metal bindingi68 – 681MagnesiumUniRule annotation
    Metal bindingi141 – 1411MagnesiumUniRule annotation
    Metal bindingi144 – 1441MagnesiumUniRule annotation

    GO - Molecular functioni

    1. crossover junction endodeoxyribonuclease activity Source: UniProtKB-HAMAP
    2. magnesium ion binding Source: UniProtKB-HAMAP
    3. nucleic acid binding Source: InterPro

    GO - Biological processi

    1. DNA recombination Source: UniProtKB-HAMAP
    2. DNA repair Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Hydrolase, Nuclease

    Keywords - Biological processi

    DNA damage, DNA recombination, DNA repair

    Keywords - Ligandi

    Magnesium, Metal-binding

    Enzyme and pathway databases

    BioCyciSGRI455632:GD3A-6098-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Crossover junction endodeoxyribonuclease RuvCUniRule annotation (EC:3.1.22.4UniRule annotation)
    Alternative name(s):
    Holliday junction nuclease RuvCUniRule annotation
    Holliday junction resolvase RuvCUniRule annotation
    Gene namesi
    Name:ruvCUniRule annotation
    Ordered Locus Names:SGR_6015
    OrganismiStreptomyces griseus subsp. griseus (strain JCM 4626 / NBRC 13350)
    Taxonomic identifieri455632 [NCBI]
    Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesStreptomycineaeStreptomycetaceaeStreptomyces
    ProteomesiUP000001685: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 204204Crossover junction endodeoxyribonuclease RuvCPRO_1000090565Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi455632.SGR_6015.

    Structurei

    3D structure databases

    ProteinModelPortaliB1W3G2.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the RuvC family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0817.
    HOGENOMiHOG000012182.
    KOiK01159.
    OMAiCHAWRGG.
    OrthoDBiEOG6RG044.

    Family and domain databases

    Gene3Di3.30.420.10. 1 hit.
    HAMAPiMF_00034. RuvC.
    InterProiIPR012337. RNaseH-like_dom.
    IPR020563. X-over_junc_endoDNase_Mg_BS.
    IPR002176. X-over_junc_endoDNase_RuvC.
    [Graphical view]
    PfamiPF02075. RuvC. 1 hit.
    [Graphical view]
    PRINTSiPR00696. RSOLVASERUVC.
    SUPFAMiSSF53098. SSF53098. 1 hit.
    TIGRFAMsiTIGR00228. ruvC. 1 hit.
    PROSITEiPS01321. RUVC. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    B1W3G2-1 [UniParc]FASTAAdd to Basket

    « Hide

    MRVLGIDPGL TRCGVGVVEG VAGRPLTMIG VGVVRTSSDA ELGDRLVAVE    50
    RGIEQWLDEH SPGYVAVERV FAQHNVRTVM GTAQASAVAM LCAARRGIPV 100
    ALHTPSEVKA AVTGSGRADK AQVGAMVTRL LRLDAPPKPA DAADALALAI 150
    CHIWRAPAQN RLQQAVAAHR TSGASRTPGA AGTPGPSRTP GAPGTSRTLK 200
    GRTA 204
    Length:204
    Mass (Da):20,971
    Last modified:May 20, 2008 - v1
    Checksum:i87227CCDF404114A
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AP009493 Genomic DNA. Translation: BAG22844.1.
    RefSeqiYP_001827527.1. NC_010572.1.

    Genome annotation databases

    EnsemblBacteriaiBAG22844; BAG22844; SGR_6015.
    GeneIDi6211160.
    KEGGisgr:SGR_6015.
    PATRICi23758869. VBIStrGri32265_6166.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AP009493 Genomic DNA. Translation: BAG22844.1 .
    RefSeqi YP_001827527.1. NC_010572.1.

    3D structure databases

    ProteinModelPortali B1W3G2.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 455632.SGR_6015.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai BAG22844 ; BAG22844 ; SGR_6015 .
    GeneIDi 6211160.
    KEGGi sgr:SGR_6015.
    PATRICi 23758869. VBIStrGri32265_6166.

    Phylogenomic databases

    eggNOGi COG0817.
    HOGENOMi HOG000012182.
    KOi K01159.
    OMAi CHAWRGG.
    OrthoDBi EOG6RG044.

    Enzyme and pathway databases

    BioCyci SGRI455632:GD3A-6098-MONOMER.

    Family and domain databases

    Gene3Di 3.30.420.10. 1 hit.
    HAMAPi MF_00034. RuvC.
    InterProi IPR012337. RNaseH-like_dom.
    IPR020563. X-over_junc_endoDNase_Mg_BS.
    IPR002176. X-over_junc_endoDNase_RuvC.
    [Graphical view ]
    Pfami PF02075. RuvC. 1 hit.
    [Graphical view ]
    PRINTSi PR00696. RSOLVASERUVC.
    SUPFAMi SSF53098. SSF53098. 1 hit.
    TIGRFAMsi TIGR00228. ruvC. 1 hit.
    PROSITEi PS01321. RUVC. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Genome sequence of the streptomycin-producing microorganism Streptomyces griseus IFO 13350."
      Ohnishi Y., Ishikawa J., Hara H., Suzuki H., Ikenoya M., Ikeda H., Yamashita A., Hattori M., Horinouchi S.
      J. Bacteriol. 190:4050-4060(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: JCM 4626 / NBRC 13350.

    Entry informationi

    Entry nameiRUVC_STRGG
    AccessioniPrimary (citable) accession number: B1W3G2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 24, 2009
    Last sequence update: May 20, 2008
    Last modified: October 1, 2014
    This is version 43 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3