B1W0I2 (RSMH_STRGG) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribosomal RNA small subunit methyltransferase H
EC=2.1.1.199

Alternative name(s):
16S rRNA m(4)C1402 methyltransferase
rRNA (cytosine-N(4)-)-methyltransferase RsmH

Gene names

Name:	rsmH
Synonyms:	mraW
Ordered Locus Names:	SGR_5413

Organism

Streptomyces griseus subsp. griseus (strain JCM 4626 / NBRC 13350) [Complete proteome] [HAMAP]

Taxonomic identifier

455632 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Streptomycineae › Streptomycetaceae › Streptomyces

Protein attributes

Sequence length	321 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Specifically methylates the N4 position of cytidine in position 1402 (C1402) of 16S rRNA By similarity. HAMAP MF_01007
Catalytic activity	S-adenosyl-L-methionine + cytosine(1402) in 16S rRNA = S-adenosyl-L-homocysteine + N(4)-methylcytosine(1402) in 16S rRNA. HAMAP MF_01007
Subcellular location	Cytoplasm By similarity HAMAP MF_01007.
Sequence similarities	Belongs to the methyltransferase superfamily. RsmH family.

Ontologies

Keywords
Biological process	rRNA processing
Cellular component	Cytoplasm
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	rRNA processing Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	methyltransferase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 321	321	Ribosomal RNA small subunit methyltransferase H HAMAP MF_01007		PRO_0000387172
Regions
	Region	40 – 42	3	S-adenosyl-L-methionine binding By similarity
Sites
	Binding site	59	1	S-adenosyl-L-methionine By similarity
	Binding site	93	1	S-adenosyl-L-methionine; via amide nitrogen By similarity
	Binding site	107	1	S-adenosyl-L-methionine By similarity
	Binding site	114	1	S-adenosyl-L-methionine By similarity

Sequences

	Sequence		Length	Mass (Da)
	B1W0I2 [UniParc]. Last modified May 20, 2008. Version 1. Checksum: 9C9C612B9ABE09B2	FASTA	321	35,048
	10 20 30 40 50 60 MSQTRHVPVM LQRCLDLLAP ALQDPAHPEP VVVDCTLGLG GHSEALLASF PTARLIALDR 70 80 90 100 110 120 DKEALRLSGE RLAPYGDRAT LVHAVYDELP EVLARLGIPK VQGVLFDLGV SSMQLDEADR 130 140 150 160 170 180 GFAYAQDAPL DMRMDQSTGM GAAEVLNTYP PGELVRILRA YGEEKQAKRI VSAVVREREK 190 200 210 220 230 240 EPFSNSARLV ELIRDALPQA AKRTGGNPAK RTFQALRIEV NGELSVLERA IPAAVDSLDV 250 260 270 280 290 300 GGRIAVLSYH SLEDRLVKQV FAAGAANTAP PGLPVVPERY QPRLKQLTRG AELPTEQEVA 310 320 ENRRAAPARL RGAQRIRAEE R

References

[1]

"Genome sequence of the streptomycin-producing microorganism Streptomyces griseus IFO 13350." Ohnishi Y., Ishikawa J., Hara H., Suzuki H., Ikenoya M., Ikeda H., Yamashita A., Hattori M., Horinouchi S. J. Bacteriol. 190:4050-4060(2008) [PubMed: 18375553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: JCM 4626 / NBRC 13350.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP009493 Genomic DNA. Translation: BAG22242.1.
RefSeq	YP_001826925.1. NC_010572.1.
3D structure databases
ProteinModelPortal	B1W0I2.
ModBase	Search...
Protein-protein interaction databases
STRING	B1W0I2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6214418.
GenomeReviews	Gene locus SGR_5413 in contig AP009493_GR.
KEGG	sgr:SGR_5413.
PATRIC	23757601. VBIStrGri32265_5544.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG302779.
OMA	NPATRAF.
ProtClustDB	PRK00050.
Family and domain databases
HAMAP	MF_01007. 16SrRNA_methyltr_H. [Tree]
InterPro	IPR002903. SAM-dep_MeTrfase_MraW. IPR023397. SAM-dep_MeTrfase_MraW_recog. [Graphical view]
Gene3D	G3DSA:1.10.150.170. G3DSA:1.10.150.170. 1 hit.
KO	K03438.
PANTHER	PTHR11265. Bact_methyltrans. 1 hit.
Pfam	PF01795. Methyltransf_5. 1 hit. [Graphical view]
PIRSF	PIRSF004486. MraW. 1 hit.
SUPFAM	SSF81799. SSF81799. 1 hit.
TIGRFAMs	TIGR00006. TIGR00006. 1 hit.
ProtoNet	Search...

Entry information

Entry name

RSMH_STRGG

Accession

Primary (citable) accession number: B1W0I2

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 3, 2009
Last sequence update:	May 20, 2008
Last modified:	January 25, 2012
This is version 31 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program