B1W041 (B1W041_STRGG) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
1,4-alpha-glucan branching enzyme GlgB HAMAP MF_00685
EC=2.4.1.18 HAMAP MF_00685

Alternative name(s):
1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferase HAMAP MF_00685
Alpha-(1->4)-glucan branching enzyme HAMAP MF_00685
Glycogen branching enzyme HAMAP MF_00685

Gene names

Name:	glgB HAMAP MF_00685 EMBL BAG18930.1
Ordered Locus Names:	SGR_2101

Organism

Streptomyces griseus subsp. griseus (strain JCM 4626 / NBRC 13350) [Complete proteome] [HAMAP]

Taxonomic identifier

455632 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Streptomycineae › Streptomycetaceae › Streptomyces

Protein attributes

Sequence length	792 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position By similarity. HAMAP MF_00685
Catalytic activity	Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain. HAMAP MF_00685 SAAS SAAS006407
Pathway	Glycan biosynthesis; glycogen biosynthesis. HAMAP MF_00685 SAAS SAAS006407
Subunit structure	Monomer By similarity. HAMAP MF_00685
Sequence similarities	Belongs to the glycosyl hydrolase 13 family. GlgB subfamily. HAMAP MF_00685

Ontologies

Keywords
Biological process	Carbohydrate metabolism Glycogen biosynthesis HAMAP MF_00685 SAAS SAAS006407 Glycogen metabolism HAMAP MF_00685
Molecular function	Glycosyltransferase HAMAP MF_00685 SAAS SAAS006407 Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycogen biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	1,4-alpha-glucan branching enzyme activity Inferred from electronic annotation. Source: HAMAP cation binding Inferred from electronic annotation. Source: InterPro hydrolase activity, hydrolyzing O-glycosyl compounds Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Sites
	Active site	466	1	Nucleophile By similarity HAMAP MF_00685
	Active site	519	1	Proton donor By similarity HAMAP MF_00685

Sequences

	Sequence		Length	Mass (Da)
	B1W041 [UniParc]. Last modified May 20, 2008. Version 1. Checksum: 527E12107EB8E284	FASTA	792	87,567
	10 20 30 40 50 60 MTARKPSRKG SRKTAEPPVE IPVEASVDVA AAPATASTTT KAARAGRARP RPADAGPPRP 70 80 90 100 110 120 RAGDGQGARP APALDGADRG RLLAGDHHAP HDVLGAHPIP GGVLVRALRP FARSVTVLAT 130 140 150 160 170 180 GLRAELHEDG DGFFSGVLPV PEVPAYRLEV AYDDNTIEVE DAYRFWPAIG ELDLHLIGEG 190 200 210 220 230 240 RHEELWRALG AEPMEHQGVS GTRFTLWAPN ARGVRVTGDF NYWDGTGYPM RSLGSTGVWE 250 260 270 280 290 300 LFLPGVGEGA LYKYDVCRPD GSHTVRADPM ARRTEVPPAT ASVVTASHHV WQDADWMAHR 310 320 330 340 350 360 GDRPVHEAPF SVYEVHLASW RPGLTYRQLA EQLPAYVKDL GFTHVELMPV SEHPFGGSWG 370 380 390 400 410 420 YQVTGFYAPT SRMGTPDDFR FLVDALHRAG IGVIMDWVPA HFPRDDWALA EFDGRPLYEH 430 440 450 460 470 480 ADPRRAAHPD WGTLEFDYGR TEVRNFLVAN ATYWCEEFHI DGLRVDAVAS MLYLDYSREE 490 500 510 520 530 540 GQWSPNEFGG RENLDAVAFL QEMNATVYRR NPGVVTIAEE STAWDGVTRP TDSGGLGFGL 550 560 570 580 590 600 KWNMGWMHDS LQYVAKEPVH RKYHHNEMTF SMVYAYSENY VLPISHDEVV HGKQALVSKM 610 620 630 640 650 660 PGDWWQRRAN HRAYLGFMWA HPGKQLLFMG QEFAQGAEWS EGHGPDWWLL DPSYEASGDH 670 680 690 700 710 720 RGVRTLVGDL NAVYGAVPAL WQRDTVPEGF SWVDGGAAED NVFAFLRYDA DGSPLLAVSH 730 740 750 760 770 780 FSPAVRADYR LGVPETGAEG WVEVLNTDAA RYGGGDVRNE EPLKAEAVPA HGRPCSVSLT 790 LPPLATVWFR PA

References

[1]

"Genome sequence of the streptomycin-producing microorganism Streptomyces griseus IFO 13350." Ohnishi Y., Ishikawa J., Hara H., Suzuki H., Ikenoya M., Ikeda H., Yamashita A., Hattori M., Horinouchi S. J. Bacteriol. 190:4050-4060(2008) [PubMed: 18375553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP009493 Genomic DNA. Translation: BAG18930.1.
RefSeq	YP_001823613.1. NC_010572.1.
3D structure databases
ProteinModelPortal	B1W041.
ModBase	Search...
Protein-protein interaction databases
STRING	B1W041.
Protein family/group databases
CAZy	CBM48. Carbohydrate-Binding Module Family 48. GH13. Glycoside Hydrolase Family 13.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6214065.
GenomeReviews	Gene locus SGR_2101 in contig AP009493_GR.
KEGG	sgr:SGR_2101.
PATRIC	23750667. VBIStrGri32265_2133.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG287139.
OMA	RVYHQNG.
ProtClustDB	PRK05402.
Family and domain databases
HAMAP	MF_00685. GlgB. [Tree]
InterPro	IPR006407. 1-4-A-glucan_branch_enz. IPR006048. A-amylase_b_C. IPR015902. Alpha_amylase. IPR013780. Glyco_hydro_13_b. IPR006047. Glyco_hydro_13_cat_dom. IPR004193. Glyco_hydro_13_N. IPR013781. Glyco_hydro_subgr_catalytic. IPR017853. Glycoside_hydrolase_SF. IPR013783. Ig-like_fold. IPR014756. Ig_E-set. [Graphical view]
Gene3D	G3DSA:2.60.40.1180. Glyco_hydro_13_b. 1 hit. G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit. G3DSA:2.60.40.10. Ig-like_fold. 2 hits.
KO	K00700.
PANTHER	PTHR10357. Alpha_amylase. 1 hit. PTHR10357:SF13. PTHR10357:SF13. 1 hit.
Pfam	PF00128. Alpha-amylase. 1 hit. PF02806. Alpha-amylase_C. 1 hit. PF02922. CBM_48. 1 hit. [Graphical view]
PIRSF	PIRSF000463. GlgB. 1 hit.
SUPFAM	SSF51445. Glyco_hydro_cat. 1 hit. SSF81296. Ig_E-set. 1 hit.
TIGRFAMs	TIGR01515. Branching_enzym. 1 hit.
ProtoNet	Search...

Entry information

Entry name

B1W041_STRGG

Accession

Primary (citable) accession number: B1W041

Entry history

Integrated into UniProtKB/TrEMBL:	May 20, 2008
Last sequence update:	May 20, 2008
Last modified:	December 14, 2011
This is version 33 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)