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B1VZ06

- SYE_STRGG

UniProt

B1VZ06 - SYE_STRGG

Protein

Glutamate--tRNA ligase

Gene

gltX

Organism
Streptomyces griseus subsp. griseus (strain JCM 4626 / NBRC 13350)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 48 (01 Oct 2014)
      Sequence version 1 (20 May 2008)
      Previous versions | rss
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    Functioni

    Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu).UniRule annotation

    Catalytic activityi

    ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu).UniRule annotation

    Cofactori

    Binds 1 zinc ion per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi122 – 1221ZincUniRule annotation
    Metal bindingi124 – 1241ZincUniRule annotation
    Metal bindingi149 – 1491ZincUniRule annotation
    Metal bindingi151 – 1511ZincUniRule annotation
    Binding sitei273 – 2731ATPUniRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-HAMAP
    2. glutamate-tRNA ligase activity Source: UniProtKB-HAMAP
    3. metal ion binding Source: UniProtKB-KW
    4. tRNA binding Source: InterPro

    GO - Biological processi

    1. glutamyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Enzyme and pathway databases

    BioCyciSGRI455632:GD3A-1945-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glutamate--tRNA ligaseUniRule annotation (EC:6.1.1.17UniRule annotation)
    Alternative name(s):
    Glutamyl-tRNA synthetaseUniRule annotation
    Short name:
    GluRSUniRule annotation
    Gene namesi
    Name:gltXUniRule annotation
    Ordered Locus Names:SGR_1932
    OrganismiStreptomyces griseus subsp. griseus (strain JCM 4626 / NBRC 13350)
    Taxonomic identifieri455632 [NCBI]
    Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesStreptomycineaeStreptomycetaceaeStreptomyces
    ProteomesiUP000001685: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 504504Glutamate--tRNA ligasePRO_0000367775Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi455632.SGR_1932.

    Structurei

    3D structure databases

    ProteinModelPortaliB1VZ06.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi25 – 3511"HIGH" regionAdd
    BLAST
    Motifi270 – 2745"KMSKS" region

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0008.
    HOGENOMiHOG000252720.
    KOiK01885.
    OMAiPEGMLNY.
    OrthoDBiEOG6DRPF7.

    Family and domain databases

    Gene3Di1.10.10.350. 1 hit.
    1.10.1160.10. 1 hit.
    3.40.50.620. 2 hits.
    HAMAPiMF_00022_B. Glu_tRNA_synth_B.
    InterProiIPR008925. aa-tRNA-synth_I_codon-bd.
    IPR020751. aa-tRNA-synth_I_codon-bd_sub2.
    IPR004527. Glu-tRNA-ligase_bac/mito.
    IPR000924. Glu/Gln-tRNA-synth.
    IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
    IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
    IPR014729. Rossmann-like_a/b/a_fold.
    [Graphical view]
    PANTHERiPTHR10119. PTHR10119. 1 hit.
    PfamiPF00749. tRNA-synt_1c. 1 hit.
    [Graphical view]
    PRINTSiPR00987. TRNASYNTHGLU.
    SUPFAMiSSF48163. SSF48163. 1 hit.
    TIGRFAMsiTIGR00464. gltX_bact. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    B1VZ06-1 [UniParc]FASTAAdd to Basket

    « Hide

    MANAPFPKLS ASLDQGRPHR VRFCPSPTGN PHVGLVRTAL FNWAFARHHQ    50
    GTLVFRIEDT DAARDSEESY QQLLDSMRWL GLDWDEGPEI GGPHAPYRQS 100
    QRMDLYKDVA EKLLAAGYAY PCYCTTEELD TRRDAARAAG KPSGYDGHCR 150
    DLTAEQKAAY EAEGRTSIVR FRMPDEAITF TDLVRGEITV QPENVPDYGI 200
    VRANGAPLYT LVNPVDDALM EITHVLRGED LLSSTPRQIA LYRALIELGI 250
    AKDTPAFGHL PYVMGEGNKK LSKRDPQASL NLYRERGFLP EGLLNYLSLL 300
    GWSIAEDRDI FSMDELVAAF DIKDVNANPA RFDLKKCEHI NAEHIRMLDV 350
    KTFTEACGPW LKAPFAPWAP EAFDAEKWTR IAPYAQTRVT VLSDITDNVD 400
    FLFLDEPVED EASWTKAMKG DPVALLTTAR ANLEAADWSD PESLKNAVLT 450
    AGEAHGLKLG KAQAPVRVAV TGRTVGLPLF ESLEILGRDR SLARVDAALA 500
    KLTA 504
    Length:504
    Mass (Da):55,894
    Last modified:May 20, 2008 - v1
    Checksum:iD7E3EF17259C5A25
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AP009493 Genomic DNA. Translation: BAG18761.1.
    RefSeqiYP_001823444.1. NC_010572.1.

    Genome annotation databases

    EnsemblBacteriaiBAG18761; BAG18761; SGR_1932.
    GeneIDi6214757.
    KEGGisgr:SGR_1932.
    PATRICi23750323. VBIStrGri32265_1961.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AP009493 Genomic DNA. Translation: BAG18761.1 .
    RefSeqi YP_001823444.1. NC_010572.1.

    3D structure databases

    ProteinModelPortali B1VZ06.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 455632.SGR_1932.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai BAG18761 ; BAG18761 ; SGR_1932 .
    GeneIDi 6214757.
    KEGGi sgr:SGR_1932.
    PATRICi 23750323. VBIStrGri32265_1961.

    Phylogenomic databases

    eggNOGi COG0008.
    HOGENOMi HOG000252720.
    KOi K01885.
    OMAi PEGMLNY.
    OrthoDBi EOG6DRPF7.

    Enzyme and pathway databases

    BioCyci SGRI455632:GD3A-1945-MONOMER.

    Family and domain databases

    Gene3Di 1.10.10.350. 1 hit.
    1.10.1160.10. 1 hit.
    3.40.50.620. 2 hits.
    HAMAPi MF_00022_B. Glu_tRNA_synth_B.
    InterProi IPR008925. aa-tRNA-synth_I_codon-bd.
    IPR020751. aa-tRNA-synth_I_codon-bd_sub2.
    IPR004527. Glu-tRNA-ligase_bac/mito.
    IPR000924. Glu/Gln-tRNA-synth.
    IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
    IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
    IPR014729. Rossmann-like_a/b/a_fold.
    [Graphical view ]
    PANTHERi PTHR10119. PTHR10119. 1 hit.
    Pfami PF00749. tRNA-synt_1c. 1 hit.
    [Graphical view ]
    PRINTSi PR00987. TRNASYNTHGLU.
    SUPFAMi SSF48163. SSF48163. 1 hit.
    TIGRFAMsi TIGR00464. gltX_bact. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Genome sequence of the streptomycin-producing microorganism Streptomyces griseus IFO 13350."
      Ohnishi Y., Ishikawa J., Hara H., Suzuki H., Ikenoya M., Ikeda H., Yamashita A., Hattori M., Horinouchi S.
      J. Bacteriol. 190:4050-4060(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: JCM 4626 / NBRC 13350.

    Entry informationi

    Entry nameiSYE_STRGG
    AccessioniPrimary (citable) accession number: B1VZ06
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 24, 2009
    Last sequence update: May 20, 2008
    Last modified: October 1, 2014
    This is version 48 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3