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Protein

Glutamate--tRNA ligase

Gene

gltX

Organism
Streptomyces griseus subsp. griseus (strain JCM 4626 / NBRC 13350)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu).UniRule annotation

Catalytic activityi

ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu).UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi122 – 1221ZincUniRule annotation
Metal bindingi124 – 1241ZincUniRule annotation
Metal bindingi149 – 1491ZincUniRule annotation
Metal bindingi151 – 1511ZincUniRule annotation
Binding sitei273 – 2731ATPUniRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. glutamate-tRNA ligase activity Source: UniProtKB-HAMAP
  3. metal ion binding Source: UniProtKB-KW
  4. tRNA binding Source: InterPro

GO - Biological processi

  1. glutamyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciSGRI455632:GD3A-1945-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate--tRNA ligaseUniRule annotation (EC:6.1.1.17UniRule annotation)
Alternative name(s):
Glutamyl-tRNA synthetaseUniRule annotation
Short name:
GluRSUniRule annotation
Gene namesi
Name:gltXUniRule annotation
Ordered Locus Names:SGR_1932
OrganismiStreptomyces griseus subsp. griseus (strain JCM 4626 / NBRC 13350)
Taxonomic identifieri455632 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesStreptomycineaeStreptomycetaceaeStreptomyces
ProteomesiUP000001685 Componenti: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 504504Glutamate--tRNA ligasePRO_0000367775Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi455632.SGR_1932.

Structurei

3D structure databases

ProteinModelPortaliB1VZ06.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi25 – 3511"HIGH" regionAdd
BLAST
Motifi270 – 2745"KMSKS" region

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0008.
HOGENOMiHOG000252720.
KOiK01885.
OMAiPAHSYLW.
OrthoDBiEOG6DRPF7.

Family and domain databases

Gene3Di1.10.10.350. 1 hit.
1.10.1160.10. 1 hit.
3.40.50.620. 2 hits.
HAMAPiMF_00022_B. Glu_tRNA_synth_B.
InterProiIPR008925. aa-tRNA-synth_I_codon-bd.
IPR020751. aa-tRNA-synth_I_codon-bd_sub2.
IPR004527. Glu-tRNA-ligase_bac/mito.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PANTHERiPTHR10119. PTHR10119. 1 hit.
PfamiPF00749. tRNA-synt_1c. 1 hit.
[Graphical view]
PRINTSiPR00987. TRNASYNTHGLU.
SUPFAMiSSF48163. SSF48163. 1 hit.
TIGRFAMsiTIGR00464. gltX_bact. 1 hit.

Sequencei

Sequence statusi: Complete.

B1VZ06-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MANAPFPKLS ASLDQGRPHR VRFCPSPTGN PHVGLVRTAL FNWAFARHHQ
60 70 80 90 100
GTLVFRIEDT DAARDSEESY QQLLDSMRWL GLDWDEGPEI GGPHAPYRQS
110 120 130 140 150
QRMDLYKDVA EKLLAAGYAY PCYCTTEELD TRRDAARAAG KPSGYDGHCR
160 170 180 190 200
DLTAEQKAAY EAEGRTSIVR FRMPDEAITF TDLVRGEITV QPENVPDYGI
210 220 230 240 250
VRANGAPLYT LVNPVDDALM EITHVLRGED LLSSTPRQIA LYRALIELGI
260 270 280 290 300
AKDTPAFGHL PYVMGEGNKK LSKRDPQASL NLYRERGFLP EGLLNYLSLL
310 320 330 340 350
GWSIAEDRDI FSMDELVAAF DIKDVNANPA RFDLKKCEHI NAEHIRMLDV
360 370 380 390 400
KTFTEACGPW LKAPFAPWAP EAFDAEKWTR IAPYAQTRVT VLSDITDNVD
410 420 430 440 450
FLFLDEPVED EASWTKAMKG DPVALLTTAR ANLEAADWSD PESLKNAVLT
460 470 480 490 500
AGEAHGLKLG KAQAPVRVAV TGRTVGLPLF ESLEILGRDR SLARVDAALA

KLTA
Length:504
Mass (Da):55,894
Last modified:May 20, 2008 - v1
Checksum:iD7E3EF17259C5A25
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP009493 Genomic DNA. Translation: BAG18761.1.
RefSeqiYP_001823444.1. NC_010572.1.

Genome annotation databases

EnsemblBacteriaiBAG18761; BAG18761; SGR_1932.
KEGGisgr:SGR_1932.
PATRICi23750323. VBIStrGri32265_1961.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP009493 Genomic DNA. Translation: BAG18761.1.
RefSeqiYP_001823444.1. NC_010572.1.

3D structure databases

ProteinModelPortaliB1VZ06.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi455632.SGR_1932.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAG18761; BAG18761; SGR_1932.
KEGGisgr:SGR_1932.
PATRICi23750323. VBIStrGri32265_1961.

Phylogenomic databases

eggNOGiCOG0008.
HOGENOMiHOG000252720.
KOiK01885.
OMAiPAHSYLW.
OrthoDBiEOG6DRPF7.

Enzyme and pathway databases

BioCyciSGRI455632:GD3A-1945-MONOMER.

Family and domain databases

Gene3Di1.10.10.350. 1 hit.
1.10.1160.10. 1 hit.
3.40.50.620. 2 hits.
HAMAPiMF_00022_B. Glu_tRNA_synth_B.
InterProiIPR008925. aa-tRNA-synth_I_codon-bd.
IPR020751. aa-tRNA-synth_I_codon-bd_sub2.
IPR004527. Glu-tRNA-ligase_bac/mito.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PANTHERiPTHR10119. PTHR10119. 1 hit.
PfamiPF00749. tRNA-synt_1c. 1 hit.
[Graphical view]
PRINTSiPR00987. TRNASYNTHGLU.
SUPFAMiSSF48163. SSF48163. 1 hit.
TIGRFAMsiTIGR00464. gltX_bact. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Genome sequence of the streptomycin-producing microorganism Streptomyces griseus IFO 13350."
    Ohnishi Y., Ishikawa J., Hara H., Suzuki H., Ikenoya M., Ikeda H., Yamashita A., Hattori M., Horinouchi S.
    J. Bacteriol. 190:4050-4060(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: JCM 4626 / NBRC 13350.

Entry informationi

Entry nameiSYE_STRGG
AccessioniPrimary (citable) accession number: B1VZ06
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: May 20, 2008
Last modified: April 1, 2015
This is version 53 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.