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B1VXF6 (SYL_STRGG) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 46. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:SGR_4973
OrganismStreptomyces griseus subsp. griseus (strain JCM 4626 / NBRC 13350) [Complete proteome] [HAMAP]
Taxonomic identifier455632 [NCBI]
Taxonomic lineageBacteriaActinobacteriaActinobacteridaeActinomycetalesStreptomycineaeStreptomycetaceaeStreptomyces

Protein attributes

Sequence length957 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 957957Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_1000091366

Regions

Motif66 – 7712"HIGH" region HAMAP-Rule MF_00049
Motif728 – 7325"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site7311ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
B1VXF6 [UniParc].

Last modified May 20, 2008. Version 1.
Checksum: 1FF90F2A1C800FB1

FASTA957106,033
        10         20         30         40         50         60 
MSETNSAAET AAPHRYTAAM AADIEARWQD FWDAEGTYEA PNPTGDLAGD PELAARPKKF 

        70         80         90        100        110        120 
IMDMFPYPSG AGLHVGHPLG YIATDVYARH QRMTGHNVLH TLGFDAFGLP AEQYAVQTGT 

       130        140        150        160        170        180 
HPRVSTEANM ENMKVQLRRL GLGHDNRRSF ATIDSEYYKW TQWIFLQIFN SWYDSEADRA 

       190        200        210        220        230        240 
RPIAELVEQF ENGTRATPDG REWGALSAAE RADLLSEYRL AYASDAPVNW SPGLGTVLAN 

       250        260        270        280        290        300 
EEVTADGRSE RGNFPVFKAK LRQWNMRITA YADRLLNDLD GLDWPEAIKL QQRNWIGRSE 

       310        320        330        340        350        360 
GARVEFPVDT AGGITVFTTR QDTLFGATYM VLAPEHDMVE RIIPAAWPEG THPVWTGGHA 

       370        380        390        400        410        420 
SPAEAVTAYR KQAAAKSDVE RQAEAKDKTG VFTGAYATNP VSGEKVPVFI ADYVLMGYGT 

       430        440        450        460        470        480 
GAIMAVPAHD ARDFAFARAF ELPMRCVVQP SDDRGTDPAT WDDAFSSYDA KLVNSANDEI 

       490        500        510        520        530        540 
SLDGLGVVEA KARITEWLKE HGVGEGTVNF RLRDWLFSRQ RYWGEPFPIV YDEDGIAHPL 

       550        560        570        580        590        600 
PESMLPLELP EVEDYSPRTF DPEDATAQPE TPLSRNADWV NVTLDLGDGA GPRKYRRETN 

       610        620        630        640        650        660 
TMPNWAGSCW YELRYLDPNN DRQLVDPSIE QYWMGPREGQ PTGGVDLYVG GAEHAVLHLL 

       670        680        690        700        710        720 
YARFWSKVLH DLGHISSAEP FHKLYNQGMI QAFVYRDSRG IAVPAAEVEE RDGAFYHAGE 

       730        740        750        760        770        780 
KVSRVLGKMG KSLKNAVTPD EICAEYGADT LRLYEMAMGP LDVSRPWDTR AVVGQYRLLQ 

       790        800        810        820        830        840 
RLWRNVVDEA TGEVTVVDTE PDEDTLRALH KAIDGVGQDM AGMRFNTAIA KVTELNNHLT 

       850        860        870        880        890        900 
KAGGPLSRSV AERLVLLIAP LAPHIAEELW RRLGHADSVV HQDFPVADPA YVVDETVTCV 

       910        920        930        940        950 
VQIKGKVRAR LEISPAITDE ELEALALADE AVVAALGGAG IRKVIVRAPK LVNIVPA 

« Hide

References

[1]"Genome sequence of the streptomycin-producing microorganism Streptomyces griseus IFO 13350."
Ohnishi Y., Ishikawa J., Hara H., Suzuki H., Ikenoya M., Ikeda H., Yamashita A., Hattori M., Horinouchi S.
J. Bacteriol. 190:4050-4060(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: JCM 4626 / NBRC 13350.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AP009493 Genomic DNA. Translation: BAG21802.1.
RefSeqYP_001826485.1. NC_010572.1.

3D structure databases

ProteinModelPortalB1VXF6.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING455632.SGR_4973.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAG21802; BAG21802; SGR_4973.
GeneID6211000.
KEGGsgr:SGR_4973.
PATRIC23756676. VBIStrGri32265_5086.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000200748.
KOK01869.
OMAYARYHRM.
OrthoDBEOG63Z74X.

Enzyme and pathway databases

BioCycSGRI455632:GD3A-5040-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 4 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_STRGG
AccessionPrimary (citable) accession number: B1VXF6
Entry history
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: May 20, 2008
Last modified: May 14, 2014
This is version 46 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries