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Protein

Leucine--tRNA ligase

Gene

leuS

Organism
Streptomyces griseus subsp. griseus (strain JCM 4626 / NBRC 13350)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei731 – 7311ATPUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. leucine-tRNA ligase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. leucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciSGRI455632:GD3A-5040-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Leucine--tRNA ligaseUniRule annotation (EC:6.1.1.4UniRule annotation)
Alternative name(s):
Leucyl-tRNA synthetaseUniRule annotation
Short name:
LeuRSUniRule annotation
Gene namesi
Name:leuSUniRule annotation
Ordered Locus Names:SGR_4973
OrganismiStreptomyces griseus subsp. griseus (strain JCM 4626 / NBRC 13350)
Taxonomic identifieri455632 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesStreptomycineaeStreptomycetaceaeStreptomyces
ProteomesiUP000001685 Componenti: Chromosome

Subcellular locationi

  1. Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 957957Leucine--tRNA ligasePRO_1000091366Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi455632.SGR_4973.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi66 – 7712"HIGH" regionAdd
BLAST
Motifi728 – 7325"KMSKS" region

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000200748.
KOiK01869.
OMAiQSSWYFL.
OrthoDBiEOG63Z74X.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 4 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B1VXF6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSETNSAAET AAPHRYTAAM AADIEARWQD FWDAEGTYEA PNPTGDLAGD
60 70 80 90 100
PELAARPKKF IMDMFPYPSG AGLHVGHPLG YIATDVYARH QRMTGHNVLH
110 120 130 140 150
TLGFDAFGLP AEQYAVQTGT HPRVSTEANM ENMKVQLRRL GLGHDNRRSF
160 170 180 190 200
ATIDSEYYKW TQWIFLQIFN SWYDSEADRA RPIAELVEQF ENGTRATPDG
210 220 230 240 250
REWGALSAAE RADLLSEYRL AYASDAPVNW SPGLGTVLAN EEVTADGRSE
260 270 280 290 300
RGNFPVFKAK LRQWNMRITA YADRLLNDLD GLDWPEAIKL QQRNWIGRSE
310 320 330 340 350
GARVEFPVDT AGGITVFTTR QDTLFGATYM VLAPEHDMVE RIIPAAWPEG
360 370 380 390 400
THPVWTGGHA SPAEAVTAYR KQAAAKSDVE RQAEAKDKTG VFTGAYATNP
410 420 430 440 450
VSGEKVPVFI ADYVLMGYGT GAIMAVPAHD ARDFAFARAF ELPMRCVVQP
460 470 480 490 500
SDDRGTDPAT WDDAFSSYDA KLVNSANDEI SLDGLGVVEA KARITEWLKE
510 520 530 540 550
HGVGEGTVNF RLRDWLFSRQ RYWGEPFPIV YDEDGIAHPL PESMLPLELP
560 570 580 590 600
EVEDYSPRTF DPEDATAQPE TPLSRNADWV NVTLDLGDGA GPRKYRRETN
610 620 630 640 650
TMPNWAGSCW YELRYLDPNN DRQLVDPSIE QYWMGPREGQ PTGGVDLYVG
660 670 680 690 700
GAEHAVLHLL YARFWSKVLH DLGHISSAEP FHKLYNQGMI QAFVYRDSRG
710 720 730 740 750
IAVPAAEVEE RDGAFYHAGE KVSRVLGKMG KSLKNAVTPD EICAEYGADT
760 770 780 790 800
LRLYEMAMGP LDVSRPWDTR AVVGQYRLLQ RLWRNVVDEA TGEVTVVDTE
810 820 830 840 850
PDEDTLRALH KAIDGVGQDM AGMRFNTAIA KVTELNNHLT KAGGPLSRSV
860 870 880 890 900
AERLVLLIAP LAPHIAEELW RRLGHADSVV HQDFPVADPA YVVDETVTCV
910 920 930 940 950
VQIKGKVRAR LEISPAITDE ELEALALADE AVVAALGGAG IRKVIVRAPK

LVNIVPA
Length:957
Mass (Da):106,033
Last modified:May 20, 2008 - v1
Checksum:i1FF90F2A1C800FB1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP009493 Genomic DNA. Translation: BAG21802.1.
RefSeqiWP_012381003.1. NC_010572.1.
YP_001826485.1. NC_010572.1.

Genome annotation databases

EnsemblBacteriaiBAG21802; BAG21802; SGR_4973.
GeneIDi6211000.
KEGGisgr:SGR_4973.
PATRICi23756676. VBIStrGri32265_5086.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP009493 Genomic DNA. Translation: BAG21802.1.
RefSeqiWP_012381003.1. NC_010572.1.
YP_001826485.1. NC_010572.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi455632.SGR_4973.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAG21802; BAG21802; SGR_4973.
GeneIDi6211000.
KEGGisgr:SGR_4973.
PATRICi23756676. VBIStrGri32265_5086.

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000200748.
KOiK01869.
OMAiQSSWYFL.
OrthoDBiEOG63Z74X.

Enzyme and pathway databases

BioCyciSGRI455632:GD3A-5040-MONOMER.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 4 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Genome sequence of the streptomycin-producing microorganism Streptomyces griseus IFO 13350."
    Ohnishi Y., Ishikawa J., Hara H., Suzuki H., Ikenoya M., Ikeda H., Yamashita A., Hattori M., Horinouchi S.
    J. Bacteriol. 190:4050-4060(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: JCM 4626 / NBRC 13350.

Entry informationi

Entry nameiSYL_STRGG
AccessioniPrimary (citable) accession number: B1VXF6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: May 20, 2008
Last modified: April 29, 2015
This is version 52 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.