B1VWJ8 (DXS_STRGG) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
1-deoxy-D-xylulose-5-phosphate synthase
EC=2.2.1.7

Alternative name(s):
1-deoxyxylulose-5-phosphate synthase
Short name=DXP synthase
Short name=DXPS

Gene names

Name:	dxs
Ordered Locus Names:	SGR_1495

Organism

Streptomyces griseus subsp. griseus (strain JCM 4626 / NBRC 13350) [Complete proteome] [HAMAP]

Taxonomic identifier

455632 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Streptomycineae › Streptomycetaceae › Streptomyces

Protein attributes

Sequence length	637 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the acyloin condensation reaction between C atoms 2 and 3 of pyruvate and glyceraldehyde 3-phosphate to yield 1-deoxy-D-xylulose-5-phosphate (DXP) By similarity. HAMAP MF_00315
Catalytic activity	Pyruvate + D-glyceraldehyde 3-phosphate = 1-deoxy-D-xylulose 5-phosphate + CO2. HAMAP MF_00315
Cofactor	Binds 1 thiamine pyrophosphate per subunit By similarity.
Pathway	Metabolic intermediate biosynthesis; 1-deoxy-D-xylulose 5-phosphate biosynthesis; 1-deoxy-D-xylulose 5-phosphate from D-glyceraldehyde 3-phosphate and pyruvate: step 1/1. HAMAP MF_00315
Subunit structure	Homodimer By similarity. HAMAP MF_00315
Sequence similarities	Belongs to the transketolase family. DXPS subfamily.

Ontologies

Keywords
Biological process	Isoprene biosynthesis Thiamine biosynthesis
Ligand	Thiamine pyrophosphate
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	terpenoid biosynthetic process Inferred from electronic annotation. Source: InterPro thiamine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	1-deoxy-D-xylulose-5-phosphate synthase activity Inferred from electronic annotation. Source: EC thiamine pyrophosphate binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 637	637	1-deoxy-D-xylulose-5-phosphate synthase HAMAP MF_00315		PRO_1000115774

Sequences

	Sequence		Length	Mass (Da)
	B1VWJ8 [UniParc]. Last modified May 20, 2008. Version 1. Checksum: 0656721E6DB64853	FASTA	637	68,195
	10 20 30 40 50 60 MDLLTRIKGP RDLDRLSLGE LDQLAQEIRT FLVDAVSKTG GHLGPNLGVV ELTIALHRVF 70 80 90 100 110 120 ESPRDKVLWD TGHQSYVHKL LTGRQDFSRL KSKGGLSGYP SRAESDHDVI ENSHASGVLG 130 140 150 160 170 180 WADGMAKANE VLRKDDHVVA VIGDGALTGG MAWEALNNIA AAKDRPLVIV VNDNERSYAP 190 200 210 220 230 240 TIGGLANHLA TLRTTDGYER FLARGKDILE RTPVVGRPLY ETLHGAKKGL KDFIAPQGMF 250 260 270 280 290 300 EDLGLKYVGP IDGHDIEALE SALQRAKRFR GPVIVHCLTE KGRGYTPALE DEADRFHAVG 310 320 330 340 350 360 KIHPDTGLPI STSGLDWTSV FGEEMVKLGE EREDIVAITA AMLQPVGLGK FEAAFPDRIY 370 380 390 400 410 420 DVGIAEQHGA ASAAGLATGG LHPVFAVYAT FLNRAFDQVL MDVALHKCGV TFVLDRAGIT 430 440 450 460 470 480 GTDGASHNGM WDMSILQCVP TLRIAAPRDA DQVRAQLREA VAVDDAPTVV RFSKGAVGPA 490 500 510 520 530 540 VKAVGRAGGM DILRRPTAAR PDVLIVSVGA LAPMCLEIAD LLDAQGISST VVDPRWVKPV 550 560 570 580 590 600 DEALAPLAER HRVVVTVEDN SRAGGVGSAV AQSLRDAGVD VPLRDFGIPP VFLDHASRGE 610 620 630 VMAEIGLTAP DIARQVTGLV AKLDGRFESR AVEPARD

References

[1]

"Genome sequence of the streptomycin-producing microorganism Streptomyces griseus IFO 13350." Ohnishi Y., Ishikawa J., Hara H., Suzuki H., Ikenoya M., Ikeda H., Yamashita A., Hattori M., Horinouchi S. J. Bacteriol. 190:4050-4060(2008) [PubMed: 18375553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: JCM 4626 / NBRC 13350.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP009493 Genomic DNA. Translation: BAG18324.1.
RefSeq	YP_001823007.1. NC_010572.1.
3D structure databases
ProteinModelPortal	B1VWJ8.
ModBase	Search...
Protein-protein interaction databases
STRING	B1VWJ8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6212975.
GenomeReviews	Gene locus SGR_1495 in contig AP009493_GR.
KEGG	sgr:SGR_1495.
PATRIC	23749401. VBIStrGri32265_1509.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG571647.
OMA	RFANEHD.
ProtClustDB	PRK12571.
Family and domain databases
HAMAP	MF_00315. DXP_synth. [Tree]
InterPro	IPR005477. Dxylulose-5-P_synthase. IPR011766. TPP_enzyme-bd_C. IPR009014. Transketo_C/Pyr-ferredox_oxred. IPR015941. Transketolase-like_C. IPR005475. Transketolase-like_Pyr-bd. IPR020826. Transketolase_BS. IPR005476. Transketolase_C. IPR005474. Transketolase_N. [Graphical view]
Gene3D	G3DSA:3.40.50.920. Transketo_C_like. 1 hit.
KO	K01662.
Pfam	PF02775. TPP_enzyme_C. 1 hit. PF02779. Transket_pyr. 1 hit. PF02780. Transketolase_C. 1 hit. [Graphical view]
SMART	SM00861. Transket_pyr. 1 hit. [Graphical view]
SUPFAM	SSF52922. Transketo_C_like. 1 hit.
TIGRFAMs	TIGR00204. Dxs. 1 hit.
PROSITE	PS00801. TRANSKETOLASE_1. 1 hit. PS00802. TRANSKETOLASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DXS_STRGG

Accession

Primary (citable) accession number: B1VWJ8

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	May 20, 2008
Last modified:	January 25, 2012
This is version 28 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program