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Protein

5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase

Gene

metE

Organism
Streptomyces griseus subsp. griseus (strain JCM 4626 / NBRC 13350)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the transfer of a methyl group from 5-methyltetrahydrofolate to homocysteine resulting in methionine formation.UniRule annotation

Catalytic activityi

5-methyltetrahydropteroyltri-L-glutamate + L-homocysteine = tetrahydropteroyltri-L-glutamate + L-methionine.UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Pathwayi: L-methionine biosynthesis via de novo pathway

This protein is involved in step 1 of the subpathway that synthesizes L-methionine from L-homocysteine (MetE route).UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. 5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase (metE)
This subpathway is part of the pathway L-methionine biosynthesis via de novo pathway, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-methionine from L-homocysteine (MetE route), the pathway L-methionine biosynthesis via de novo pathway and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi656 – 6561ZincUniRule annotation
Metal bindingi658 – 6581ZincUniRule annotation
Metal bindingi741 – 7411ZincUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

Amino-acid biosynthesis, Methionine biosynthesis

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BioCyciSGRI455632:GD3A-1216-MONOMER.
UniPathwayiUPA00051; UER00082.

Names & Taxonomyi

Protein namesi
Recommended name:
5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferaseUniRule annotation (EC:2.1.1.14UniRule annotation)
Alternative name(s):
Cobalamin-independent methionine synthaseUniRule annotation
Methionine synthase, vitamin-B12 independent isozymeUniRule annotation
Gene namesi
Name:metEUniRule annotation
Ordered Locus Names:SGR_1212
OrganismiStreptomyces griseus subsp. griseus (strain JCM 4626 / NBRC 13350)
Taxonomic identifieri455632 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaStreptomycetalesStreptomycetaceaeStreptomyces
Proteomesi
  • UP000001685 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 7747745-methyltetrahydropteroyltriglutamate--homocysteine methyltransferasePRO_1000097846Add
BLAST

Proteomic databases

PRIDEiB1VV57.

Interactioni

Protein-protein interaction databases

STRINGi455632.SGR_1212.

Structurei

3D structure databases

ProteinModelPortaliB1VV57.
SMRiB1VV57. Positions 12-768.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the vitamin-B12 independent methionine synthase family.UniRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiENOG4107QYE. Bacteria.
COG0620. LUCA.
HOGENOMiHOG000246221.
KOiK00549.
OMAiRFGWVQS.

Family and domain databases

HAMAPiMF_00172. Meth_synth. 1 hit.
InterProiIPR013215. Cbl-indep_Met_Synth_N.
IPR006276. Cobalamin-indep_Met_synthase.
IPR002629. Met_Synth_C/arc.
[Graphical view]
PfamiPF08267. Meth_synt_1. 1 hit.
PF01717. Meth_synt_2. 1 hit.
[Graphical view]
PIRSFiPIRSF000382. MeTrfase_B12_ind. 1 hit.
TIGRFAMsiTIGR01371. met_syn_B12ind. 1 hit.

Sequencei

Sequence statusi: Complete.

B1VV57-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTAKPAAAAA RATVYGYPRQ GPHRELKKAI EGYWKGRVDA DALRETAAEL
60 70 80 90 100
RRGTWQQLAE AGVHEVPTGD FSYYDHVLDT SVMVGAVPER HRASVEADAL
110 120 130 140 150
DGYFAMARGT QDVAPLEMTK WFDTNYHYLV PELGPDTVFS ADSAKQVAEF
160 170 180 190 200
REALALGWTP RPVLVGPLTY LLLAKAAPGV AADFEPLTLL DRLLPVYAEI
210 220 230 240 250
LADLRAAGAT WVQLDEPALV QDRTPAELNA AARAYRELGG RADRPQLLVA
260 270 280 290 300
SYFGRLGEAL AVLAKAPVDG LALDFTESGA GNLDDLAAAG GLPGKRLVAG
310 320 330 340 350
VVNGRNIWIN DYAKSLATLG TLLGLADRVD VSASCSLLHV PLDADAERGI
360 370 380 390 400
DPETARWLAF AKQKTEEIVV LARGLGSGTD AIASELAANR ADLASRTGAA
410 420 430 440 450
LTRDPAVRAR TAAVTEADGR RSQPYAERSA AQRAGLGLPL LPTTTIGSFP
460 470 480 490 500
QTTELRTARA ELRAGRIDEA EYEERVKDEI REVLSFQEKA GIDVLVHGEP
510 520 530 540 550
ERNDMVQYFA EQLDGYLATQ HGWVQSYGTR YVRPPVLAGD ISRPEPMTVR
560 570 580 590 600
WTTYAQSLTA RPVKGMLTGP VTMLAWSFVR DDQPLGDTAR QVALALRDEV
610 620 630 640 650
NDLEANGTSV IQVDEPALRE TLPLRAAEHA AYLEWATESF RIATSGVRPD
660 670 680 690 700
TQIHTHMCYA EFGDIVQAID DLDADVISLE AARSHMQVAR ELAAHGYPRE
710 720 730 740 750
AGPGVYDIHS PRIPGAEEAA ALLRKGLEAI PAERLWVNPD CGLKTRGWPE
760 770
TRASLENLVA AARQIRAELP TGAA
Length:774
Mass (Da):83,741
Last modified:May 20, 2008 - v1
Checksum:i35F4E4BB7104E31C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP009493 Genomic DNA. Translation: BAG18041.1.
RefSeqiWP_012378383.1. NC_010572.1.

Genome annotation databases

EnsemblBacteriaiBAG18041; BAG18041; SGR_1212.
GeneIDi6215208.
KEGGisgr:SGR_1212.
PATRICi23748809. VBIStrGri32265_1213.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP009493 Genomic DNA. Translation: BAG18041.1.
RefSeqiWP_012378383.1. NC_010572.1.

3D structure databases

ProteinModelPortaliB1VV57.
SMRiB1VV57. Positions 12-768.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi455632.SGR_1212.

Proteomic databases

PRIDEiB1VV57.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAG18041; BAG18041; SGR_1212.
GeneIDi6215208.
KEGGisgr:SGR_1212.
PATRICi23748809. VBIStrGri32265_1213.

Phylogenomic databases

eggNOGiENOG4107QYE. Bacteria.
COG0620. LUCA.
HOGENOMiHOG000246221.
KOiK00549.
OMAiRFGWVQS.

Enzyme and pathway databases

UniPathwayiUPA00051; UER00082.
BioCyciSGRI455632:GD3A-1216-MONOMER.

Family and domain databases

HAMAPiMF_00172. Meth_synth. 1 hit.
InterProiIPR013215. Cbl-indep_Met_Synth_N.
IPR006276. Cobalamin-indep_Met_synthase.
IPR002629. Met_Synth_C/arc.
[Graphical view]
PfamiPF08267. Meth_synt_1. 1 hit.
PF01717. Meth_synt_2. 1 hit.
[Graphical view]
PIRSFiPIRSF000382. MeTrfase_B12_ind. 1 hit.
TIGRFAMsiTIGR01371. met_syn_B12ind. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiMETE_STRGG
AccessioniPrimary (citable) accession number: B1VV57
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: May 20, 2008
Last modified: September 7, 2016
This is version 58 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.