B1VV57 (METE_STRGG) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase
EC=2.1.1.14

Alternative name(s):
Cobalamin-independent methionine synthase
Methionine synthase, vitamin-B12 independent isozyme

Gene names

Name:	metE
Ordered Locus Names:	SGR_1212

Organism

Streptomyces griseus subsp. griseus (strain JCM 4626 / NBRC 13350) [Complete proteome] [HAMAP]

Taxonomic identifier

455632 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Streptomycineae › Streptomycetaceae › Streptomyces

Protein attributes

Sequence length	774 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the transfer of a methyl group from 5-methyltetrahydrofolate to homocysteine resulting in methionine formation By similarity. HAMAP MF_00172
Catalytic activity	5-methyltetrahydropteroyltri-L-glutamate + L-homocysteine = tetrahydropteroyltri-L-glutamate + L-methionine. HAMAP MF_00172
Cofactor	Binds 1 zinc ion per subunit By similarity. HAMAP MF_00172
Pathway	Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-methionine from L-homocysteine (MetE route): step 1/1. HAMAP MF_00172
Sequence similarities	Belongs to the vitamin-B12 independent methionine synthase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Methionine biosynthesis
Domain	Repeat
Ligand	Metal-binding Zinc
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	methionine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	5-methyltetrahydropteroyltriglutamate-homocysteine S-methyltransferase activity Inferred from electronic annotation. Source: EC zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 774	774	5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase HAMAP MF_00172		PRO_1000097846
Sites
	Metal binding	656	1	Zinc By similarity
	Metal binding	658	1	Zinc By similarity
	Metal binding	741	1	Zinc By similarity

Sequences

	Sequence		Length	Mass (Da)
	B1VV57 [UniParc]. Last modified May 20, 2008. Version 1. Checksum: 35F4E4BB7104E31C	FASTA	774	83,741
	10 20 30 40 50 60 MTAKPAAAAA RATVYGYPRQ GPHRELKKAI EGYWKGRVDA DALRETAAEL RRGTWQQLAE 70 80 90 100 110 120 AGVHEVPTGD FSYYDHVLDT SVMVGAVPER HRASVEADAL DGYFAMARGT QDVAPLEMTK 130 140 150 160 170 180 WFDTNYHYLV PELGPDTVFS ADSAKQVAEF REALALGWTP RPVLVGPLTY LLLAKAAPGV 190 200 210 220 230 240 AADFEPLTLL DRLLPVYAEI LADLRAAGAT WVQLDEPALV QDRTPAELNA AARAYRELGG 250 260 270 280 290 300 RADRPQLLVA SYFGRLGEAL AVLAKAPVDG LALDFTESGA GNLDDLAAAG GLPGKRLVAG 310 320 330 340 350 360 VVNGRNIWIN DYAKSLATLG TLLGLADRVD VSASCSLLHV PLDADAERGI DPETARWLAF 370 380 390 400 410 420 AKQKTEEIVV LARGLGSGTD AIASELAANR ADLASRTGAA LTRDPAVRAR TAAVTEADGR 430 440 450 460 470 480 RSQPYAERSA AQRAGLGLPL LPTTTIGSFP QTTELRTARA ELRAGRIDEA EYEERVKDEI 490 500 510 520 530 540 REVLSFQEKA GIDVLVHGEP ERNDMVQYFA EQLDGYLATQ HGWVQSYGTR YVRPPVLAGD 550 560 570 580 590 600 ISRPEPMTVR WTTYAQSLTA RPVKGMLTGP VTMLAWSFVR DDQPLGDTAR QVALALRDEV 610 620 630 640 650 660 NDLEANGTSV IQVDEPALRE TLPLRAAEHA AYLEWATESF RIATSGVRPD TQIHTHMCYA 670 680 690 700 710 720 EFGDIVQAID DLDADVISLE AARSHMQVAR ELAAHGYPRE AGPGVYDIHS PRIPGAEEAA 730 740 750 760 770 ALLRKGLEAI PAERLWVNPD CGLKTRGWPE TRASLENLVA AARQIRAELP TGAA

References

[1]

"Genome sequence of the streptomycin-producing microorganism Streptomyces griseus IFO 13350." Ohnishi Y., Ishikawa J., Hara H., Suzuki H., Ikenoya M., Ikeda H., Yamashita A., Hattori M., Horinouchi S. J. Bacteriol. 190:4050-4060(2008) [PubMed: 18375553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: JCM 4626 / NBRC 13350.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP009493 Genomic DNA. Translation: BAG18041.1.
RefSeq	YP_001822724.1. NC_010572.1.
3D structure databases
ProteinModelPortal	B1VV57.
SMR	B1VV57. Positions 12-768.
ModBase	Search...
Protein-protein interaction databases
STRING	B1VV57.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6215208.
GenomeReviews	Gene locus SGR_1212 in contig AP009493_GR.
KEGG	sgr:SGR_1212.
PATRIC	23748809. VBIStrGri32265_1213.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG287495.
OMA	RNIWRAN.
ProtClustDB	PRK05222.
Family and domain databases
HAMAP	MF_00172. Meth_synth. [Tree]
InterPro	IPR013215. Cbl-indep_Met_Synth_N. IPR006276. Cobalamin-indep_Met_synthase. IPR002629. Methionine_synth. [Graphical view]
KO	K00549.
Pfam	PF08267. Meth_synt_1. 1 hit. PF01717. Meth_synt_2. 1 hit. [Graphical view]
PIRSF	PIRSF000382. MeTrfase_B12_ind. 1 hit.
TIGRFAMs	TIGR01371. Met_syn_B12ind. 1 hit.
ProtoNet	Search...

Entry information

Entry name

METE_STRGG

Accession

Primary (citable) accession number: B1VV57

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	May 20, 2008
Last modified:	January 25, 2012
This is version 31 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program