B1VUG0 (B1VUG0_STRGG) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
4-diphosphocytidyl-2-C-methyl-D-erythritol kinase HAMAP MF_00061
Short name=CMK HAMAP MF_00061
EC=2.7.1.148 HAMAP MF_00061

Alternative name(s):
4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinase HAMAP MF_00061

Gene names

Name:	ispE HAMAP MF_00061
Ordered Locus Names:	SGR_4357

Organism

Streptomyces griseus subsp. griseus (strain JCM 4626 / NBRC 13350) [Complete proteome] [HAMAP]

Taxonomic identifier

455632 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Streptomycineae › Streptomycetaceae › Streptomyces

Protein attributes

Sequence length	302 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol By similarity. HAMAP MF_00061
Catalytic activity	ATP + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol = ADP + 2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol. HAMAP MF_00061
Pathway	Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 3/6. HAMAP MF_00061
Sequence similarities	Belongs to the GHMP kinase family. IspE subfamily. HAMAP MF_00061

Ontologies

Keywords
Biological process	Isoprene biosynthesis HAMAP MF_00061
Ligand	ATP-binding HAMAP MF_00061 RuleBase RU003563 Nucleotide-binding
Molecular function	Kinase HAMAP MF_00061 RuleBase RU003563 EMBL BAG21186.1 Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	terpenoid biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase activity Inferred from electronic annotation. Source: HAMAP ATP binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Regions
	Nucleotide binding	100 – 110	11	ATP By similarity HAMAP MF_00061
Sites
	Active site	15	1	By similarity HAMAP MF_00061
	Active site	142	1	By similarity HAMAP MF_00061

Sequences

	Sequence		Length	Mass (Da)
	B1VUG0 [UniParc]. Last modified May 20, 2008. Version 1. Checksum: 2CE0F2EE5255DB79	FASTA	302	29,852
	10 20 30 40 50 60 MSTVRTGVTV RVPAKVNVQL AVGAARPDGF HDLANVFLAV GLYDEVTAAP ADELRVTCSG 70 80 90 100 110 120 PDAGQVPLDR TNLAARAALA LAERYGIDPA VHLHIAKDIP VAGGMAGGSA DGAAALVACD 130 140 150 160 170 180 ALWGTGASRD ELLAICAELG SDVPFSLVGG AALGTGRGEK LTPIEVGGTF HWVFAVADGG 190 200 210 220 230 240 LSTPAVYGEF DRLTAGTEVP EPTASPALLA ALRSGDPGAL AGALSNDLQP AALSLRPSLA 250 260 270 280 290 300 ETLAAGTEAG APAALVSGSG PTTAFLVPDA ETAEKVAAAL LTSGTCRNAR VAASPAPGAH VI

References

[1]

"Genome sequence of the streptomycin-producing microorganism Streptomyces griseus IFO 13350." Ohnishi Y., Ishikawa J., Hara H., Suzuki H., Ikenoya M., Ikeda H., Yamashita A., Hattori M., Horinouchi S. J. Bacteriol. 190:4050-4060(2008) [PubMed: 18375553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP009493 Genomic DNA. Translation: BAG21186.1.
RefSeq	YP_001825869.1. NC_010572.1.
3D structure databases
ProteinModelPortal	B1VUG0.
ModBase	Search...
Protein-protein interaction databases
STRING	B1VUG0.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6212047.
GenomeReviews	Gene locus SGR_4357 in contig AP009493_GR.
KEGG	sgr:SGR_4357.
PATRIC	23755356. VBIStrGri32265_4442.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG734593.
OMA	KINLHLG.
ProtClustDB	PRK03188.
Family and domain databases
HAMAP	MF_00061. IspE. [Tree]
InterPro	IPR006204. GHMP_kinase. IPR013750. GHMP_kinase_C. IPR004424. IspE. IPR020568. Ribosomal_S5_D2-typ_fold. IPR014721. Ribosomal_S5_D2-typ_fold_subgr. [Graphical view]
Gene3D	G3DSA:3.30.230.10. Ribosomal_S5_D2-type_fold. 1 hit.
KO	K00919.
Pfam	PF08544. GHMP_kinases_C. 1 hit. PF00288. GHMP_kinases_N. 1 hit. [Graphical view]
PIRSF	PIRSF010376. IspE. 1 hit.
SUPFAM	SSF54211. Ribosomal_S5_D2-typ_fold. 1 hit.
TIGRFAMs	TIGR00154. IspE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

B1VUG0_STRGG

Accession

Primary (citable) accession number: B1VUG0

Entry history

Integrated into UniProtKB/TrEMBL:	May 20, 2008
Last sequence update:	May 20, 2008
Last modified:	December 14, 2011
This is version 32 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)