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Protein

D-inositol 3-phosphate glycosyltransferase

Gene

mshA

Organism
Streptomyces griseus subsp. griseus (strain JCM 4626 / NBRC 13350)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the transfer of a N-acetyl-glucosamine moiety to 1D-myo-inositol 3-phosphate to produce 1D-myo-inositol 2-acetamido-2-deoxy-glucopyranoside 3-phosphate in the mycothiol biosynthesis pathway.UniRule annotation

Catalytic activityi

UDP-N-acetyl-D-glucosamine + 1D-myo-inositol 3-phosphate = UDP + 1-O-(2-acetamido-2-deoxy-alpha-D-glucopyranosyl)-1D-myo-inositol 3-phosphate.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei481D-inositol 3-phosphateUniRule annotation1
Binding sitei62UDP-GlcNAc; via amide nitrogenUniRule annotation1
Binding sitei1171D-inositol 3-phosphateUniRule annotation1
Binding sitei1501D-inositol 3-phosphateUniRule annotation1
Binding sitei1741D-inositol 3-phosphateUniRule annotation1
Binding sitei1941D-inositol 3-phosphateUniRule annotation1
Binding sitei282UDP-GlcNAcUniRule annotation1
Binding sitei287UDP-GlcNAcUniRule annotation1
Binding sitei348UDP-GlcNAc; via amide nitrogen and carbonyl oxygenUniRule annotation1
Metal bindingi357Magnesium; via carbonyl oxygenUniRule annotation1
Metal bindingi358Magnesium; via carbonyl oxygenUniRule annotation1
Metal bindingi360Magnesium; via carbonyl oxygenUniRule annotation1
Binding sitei370UDP-GlcNAcUniRule annotation1
Binding sitei378UDP-GlcNAcUniRule annotation1
Metal bindingi384MagnesiumUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Ligandi

Magnesium, Metal-binding

Protein family/group databases

CAZyiGT4. Glycosyltransferase Family 4.

Names & Taxonomyi

Protein namesi
Recommended name:
D-inositol 3-phosphate glycosyltransferase (EC:2.4.1.250UniRule annotation)
Alternative name(s):
N-acetylglucosamine-inositol-phosphate N-acetylglucosaminyltransferaseUniRule annotation
Short name:
GlcNAc-Ins-P N-acetylglucosaminyltransferaseUniRule annotation
Gene namesi
Name:mshAUniRule annotation
Ordered Locus Names:SGR_3993
OrganismiStreptomyces griseus subsp. griseus (strain JCM 4626 / NBRC 13350)
Taxonomic identifieri455632 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaStreptomycetalesStreptomycetaceaeStreptomyces
Proteomesi
  • UP000001685 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004001631 – 472D-inositol 3-phosphate glycosyltransferaseAdd BLAST472

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi455632.SGR_3993.

Structurei

3D structure databases

ProteinModelPortaliB1VS68.
SMRiB1VS68.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni54 – 55UDP-GlcNAc bindingUniRule annotation2
Regioni59 – 641D-inositol 3-phosphate bindingUniRule annotation6

Sequence similaritiesi

Belongs to the glycosyltransferase group 1 family. MshA subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4107R5M. Bacteria.
COG0438. LUCA.
HOGENOMiHOG000077288.
KOiK15521.
OMAiHTMAKVK.

Family and domain databases

HAMAPiMF_01695. MshA. 1 hit.
InterProiIPR001296. Glyco_trans_1.
IPR028098. Glyco_trans_4-like_N.
IPR017814. Mycothiol_biosynthesis_MshA.
[Graphical view]
PfamiPF13439. Glyco_transf_4. 1 hit.
PF00534. Glycos_transf_1. 1 hit.
[Graphical view]
TIGRFAMsiTIGR03449. mycothiol_MshA. 1 hit.

Sequencei

Sequence statusi: Complete.

B1VS68-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPRSRRTDEV SQYVSRLGST RTAARLRFPG GFSGASRKPR RVAMLSVHTS
60 70 80 90 100
PLHQPGTGDA GGMNVYIVEL AKRLAAINIE VEIFTRATTG SLAPTVELAP
110 120 130 140 150
GVLVRHVDAG PYEGLAKEEL PAQLCAFTHG VMQAWAGQRP GYYDLVHSHY
160 170 180 190 200
WLSGQVGWLA AQRWGVPLVH AMHTMAKVKN AALAEGDTPE PAARVIGETQ
210 220 230 240 250
IVNASDRLIA NTAEEADELV RFYDADPAAV AVVHPGVNLD RFRPFPQGSD
260 270 280 290 300
ELRPGNAPSG RAAARARLGL PQDALIPLFA GRIQPLKAPD VLLRAVAVLL
310 320 330 340 350
DRDPSLRSRI VVPVVGGPSG SGLAKPEGLQ KLAARLGIAD VVRFHPPVGQ
360 370 380 390 400
ERLADWFRAA SVLVMPSYSE SFGLVAIEAQ ATGTPVVAAA VGGLPVAVRD
410 420 430 440 450
GVGGFLVQGH EPEAYARALG RFADAPELVE RMGAAAAAHA QSFGWDTAAS
460 470
ATADVYTAAL SDHRRRARAH HG
Length:472
Mass (Da):50,041
Last modified:May 20, 2008 - v1
Checksum:iFD7FB35C5B581931
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP009493 Genomic DNA. Translation: BAG20822.1.

Genome annotation databases

EnsemblBacteriaiBAG20822; BAG20822; SGR_3993.
KEGGisgr:SGR_3993.
PATRICi23754590. VBIStrGri32265_4065.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP009493 Genomic DNA. Translation: BAG20822.1.

3D structure databases

ProteinModelPortaliB1VS68.
SMRiB1VS68.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi455632.SGR_3993.

Protein family/group databases

CAZyiGT4. Glycosyltransferase Family 4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAG20822; BAG20822; SGR_3993.
KEGGisgr:SGR_3993.
PATRICi23754590. VBIStrGri32265_4065.

Phylogenomic databases

eggNOGiENOG4107R5M. Bacteria.
COG0438. LUCA.
HOGENOMiHOG000077288.
KOiK15521.
OMAiHTMAKVK.

Family and domain databases

HAMAPiMF_01695. MshA. 1 hit.
InterProiIPR001296. Glyco_trans_1.
IPR028098. Glyco_trans_4-like_N.
IPR017814. Mycothiol_biosynthesis_MshA.
[Graphical view]
PfamiPF13439. Glyco_transf_4. 1 hit.
PF00534. Glycos_transf_1. 1 hit.
[Graphical view]
TIGRFAMsiTIGR03449. mycothiol_MshA. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiMSHA_STRGG
AccessioniPrimary (citable) accession number: B1VS68
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 2, 2010
Last sequence update: May 20, 2008
Last modified: November 2, 2016
This is version 51 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.