B1VQW8 (SYD_STRGG) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Aspartate--tRNA ligase
EC=6.1.1.12

Alternative name(s):
Aspartyl-tRNA synthetase
Short name=AspRS

Gene names

Name:	aspS
Ordered Locus Names:	SGR_3790

Organism

Streptomyces griseus subsp. griseus (strain JCM 4626 / NBRC 13350) [Complete proteome] [HAMAP]

Taxonomic identifier

455632 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Streptomycineae › Streptomycetaceae › Streptomyces

Protein attributes

Sequence length	599 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-aspartate + tRNA(Asp) = AMP + diphosphate + L-aspartyl-tRNA(Asp). HAMAP MF_00044_B
Subunit structure	Homodimer By similarity. HAMAP MF_00044_B
Subcellular location	Cytoplasm By similarity HAMAP MF_00044_B.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	tRNA aminoacylation for protein translation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW aspartate-tRNA ligase activity Inferred from electronic annotation. Source: EC nucleic acid binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 599	599	Aspartate--tRNA ligase HAMAP MF_00044_B		PRO_1000091046

Sequences

	Sequence		Length	Mass (Da)
	B1VQW8 [UniParc]. Last modified May 20, 2008. Version 1. Checksum: F5A4907E153ECC20	FASTA	599	66,773
	10 20 30 40 50 60 MHRYRSHTCG ELRASDVGTD VRLSGWLHNR RDLGGILFID LRDHYGLVQL VARPGTPGND 70 80 90 100 110 120 ALAKLTKETV VRIDGKVSAR GADNVNPELP TGEIEIEVTE VEVLGEAGPL PFTINTEDGV 130 140 150 160 170 180 NEERRLEYRF LDLRRERMHR NIMLRSAVIA SIRSKMVALG FNEMATPILT ATSPEGARDF 190 200 210 220 230 240 VVPSRLNPGK FYALPQAPQQ FKQLLMISGF DRYFQIAPCF RDEDARADRS PGEFYQLDVE 250 260 270 280 290 300 MSFVEQEDVF QPIEKLMTEL FTEFGNGREV TSPFPRIPFR ESMLKYGNDK PDLRAKLELV 310 320 330 340 350 360 DISDVFADSG FKAFAGKHVR ALPVPDTAGQ SRKFFDGLGE YAVEHGAKGL AWVRVGEDGT 370 380 390 400 410 420 LAGPIAKFLT ETDVKTLTER LSLVPGHAVF FGAGEFDEVS KIMSAVRVEA AKRAGHFEEG 430 440 450 460 470 480 VFRFCWIVDF PMYEKDEETG KIDFSHNPFS MPQGGLADLE EKDPLDILAW QYDIVCNGIE 490 500 510 520 530 540 LSSGAIRNHE PELMLKAFEI AGYDRETVEH EFAGMLRAFR LGAPPHGGIA PGVDRIVMLL 550 560 570 580 590 ADEPNIRETI AFPLNGNAQD LMMGAPTELD ESRLRELNIQ LRKPVAAKGA NAPEKTAEK

References

[1]

"Genome sequence of the streptomycin-producing microorganism Streptomyces griseus IFO 13350." Ohnishi Y., Ishikawa J., Hara H., Suzuki H., Ikenoya M., Ikeda H., Yamashita A., Hattori M., Horinouchi S. J. Bacteriol. 190:4050-4060(2008) [PubMed: 18375553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: JCM 4626 / NBRC 13350.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP009493 Genomic DNA. Translation: BAG20619.1.
RefSeq	YP_001825302.1. NC_010572.1.
3D structure databases
ProteinModelPortal	B1VQW8.
ModBase	Search...
Protein-protein interaction databases
STRING	B1VQW8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6209781.
GenomeReviews	Gene locus SGR_3790 in contig AP009493_GR.
KEGG	sgr:SGR_3790.
PATRIC	23754154. VBIStrGri32265_3859.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG396032.
OMA	YQLDVEM.
ProtClustDB	PRK00476.
Family and domain databases
HAMAP	MF_00044_B. Asp_tRNA_synth_B. [Tree]
InterPro	IPR004364. aa-tRNA-synt_II. IPR018150. aa-tRNA-synt_II-like. IPR006195. aa-tRNA-synth_II. IPR004524. Asp-tRNA-synth_IIb_bac/mt. IPR002312. Asp/Asn-tRNA-synth_IIb. IPR004115. GAD_dom. IPR012340. NA-bd_OB-fold. IPR016027. NA-bd_OB-fold-like. IPR004365. NA-bd_OB_tRNA-helicase. [Graphical view]
Gene3D	G3DSA:3.30.1360.30. GAD_dom. 1 hit. G3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit.
KO	K01876.
PANTHER	PTHR22594. aa-tRNA-synt_II. 1 hit. PTHR22594:SF5. AspS_bac. 1 hit.
Pfam	PF02938. GAD. 1 hit. PF00152. tRNA-synt_2. 1 hit. PF01336. tRNA_anti. 1 hit. [Graphical view]
PRINTS	PR01042. TRNASYNTHASP.
SUPFAM	SSF50249. Nucleic_acid_OB. 1 hit. SSF55261. SSF55261. 1 hit.
TIGRFAMs	TIGR00459. AspS_bact. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYD_STRGG

Accession

Primary (citable) accession number: B1VQW8

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	May 20, 2008
Last modified:	January 25, 2012
This is version 30 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program