Reviewed, UniProtKB/Swiss-Prot B1VMX8 (RLMG_STRGG)

Names and origin

Protein names

Recommended name:
    Ribosomal RNA large subunit methyltransferase G
    EC=2.1.1.52
Alternative name(s):
    rRNA (guanine-N(2)-)-methyltransferase rlmG
    23S rRNA m2G1835 methyltransferase

Gene names

Name:	rlmG
Ordered Locus Names:	SGR_6774

Organism

Streptomyces griseus subsp. griseus (strain JCM 4626 / NBRC 13350) [Complete proteome] [HAMAP]

Taxonomic identifier

455632 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Streptomycineae › Streptomycetaceae › Streptomyces

Protein attributes

Sequence length	384 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Specifically methylates the guanosine in position 1835 (m2G1835) of 23S rRNA By similarity.
Catalytic activity	S-adenosyl-L-methionine + rRNA = S-adenosyl-L-homocysteine + rRNA containing N(2)-methylguanine. HAMAP MF_01859
Subcellular location	Cytoplasm Potential.
Sequence similarities	Belongs to the methyltransferase superfamily. RlmG family.

Ontologies

Keywords
Biological process	rRNA processing
Cellular component	Cytoplasm
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	rRNA methylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	rRNA (guanine-N2-)-methyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 384	384	Ribosomal RNA large subunit methyltransferase G HAMAP MF_01859		PRO_0000366529

Sequences

	Sequence		Length	Mass (Da)
	B1VMX8-1 [UniParc]. Last modified May 20, 2008. Version 1. Checksum: 166835FBE5E59C23	FASTA	384	41,294
	10 20 30 40 50 60 MNRFTTSQGA FELARFPEHP RDPFRAWDAA DDYLLQQLTD PGTGPVDLSG TVAVVGDRWG 70 80 90 100 110 120 ALATALAAHR PVQISDSYLA RRATLANLAR NGIDEDAVRL LSSRDTPPDR IDVLLVRVPK 130 140 150 160 170 180 SLAFLEDQLH RLAPAVHSGT VVIGTGMVKE IHTSTLKIFE RIIGPTRTSL AVRKARLIFS 190 200 210 220 230 240 TPDPALPRTL SPWPYRYELP ADVGPVAGLT AVNHAGIFCA ERLDIGTRFF LKHLPTRSGA 250 260 270 280 290 300 VRVVDLGCGN GVLGLSAAVA NPDAHLTFVD ESYGAVASAE ETFRAGAPAG AKADFLVGDG 310 320 330 340 350 360 LADLDPGSVD LVLNNPPFHS HLATTDATAR TMFAGAKTAL RRGGELWVVG NRHLSYHTHL 370 380 RRIFGNCTTV AGDPKFVVLR AVKR

References

[1]

"Genome sequence of the streptomycin-producing microorganism Streptomyces griseus IFO 13350." Ohnishi Y., Ishikawa J., Hara H., Suzuki H., Ikenoya M., Ikeda H., Yamashita A., Hattori M., Horinouchi S. J. Bacteriol. 190:4050-4060(2008) [PubMed: 18375553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AP009493 Genomic DNA. Translation: BAG23603.1.
RefSeq	YP_001828286.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	6212127.
GenomeReviews	Gene locus SGR_6774 in contig AP009493_GR.
KEGG	sgr:SGR_6774.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	NRHLGYH.
Family and domain databases
HAMAP	MF_01859. [Tree]
InterPro	IPR017237. rRNA_m2G-MeTrfase_RsmD. IPR007848. Small_mtfrase. [Graphical view]
Pfam	PF05175. MTS. 1 hit. [Graphical view]
PIRSF	PIRSF037565. RRNA_m2G_Mtase_RsmD_prd. 1 hit.
ProtoNet	Search...

Entry information

Entry name

RLMG_STRGG

Accession

Primary (citable) accession number: B1VMX8

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	May 20, 2008
Last modified:	November 3, 2009
This is version 12 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)