B1VGB6 (SYP_CORU7) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Proline--tRNA ligase
EC=6.1.1.15

Alternative name(s):
Prolyl-tRNA synthetase
Short name=ProRS

Gene names

Name:	proS
Ordered Locus Names:	cu0845

Organism

Corynebacterium urealyticum (strain ATCC 43042 / DSM 7109) [Complete proteome] [HAMAP]

Taxonomic identifier

504474 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Corynebacteriaceae › Corynebacterium

Protein attributes

Sequence length	592 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction: proline is first activated by ATP to form Pro-AMP and then transferred to the acceptor end of tRNA(Pro). As ProRS can inadvertently accommodate and process non-cognate amino acids such as alanine and cysteine, to avoid such errors it has two additional distinct editing activities against alanine. One activity is designated as 'pretransfer' editing and involves the tRNA(Pro)-independent hydrolysis of activated Ala-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Ala-tRNA(Pro). The misacylated Cys-tRNA(Pro) is not edited by ProRS By similarity. HAMAP MF_01569
Catalytic activity	ATP + L-proline + tRNA(Pro) = AMP + diphosphate + L-prolyl-tRNA(Pro). HAMAP MF_01569
Subunit structure	Homodimer By similarity. HAMAP MF_01569
Subcellular location	Cytoplasm By similarity HAMAP MF_01569.
Domain	Consists of three domains: the N-terminal catalytic domain, the editing domain and the C-terminal anticodon-binding domain By similarity. HAMAP MF_01569
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 1 subfamily.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	prolyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW proline-tRNA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 592	592	Proline--tRNA ligase HAMAP MF_01569		PRO_1000199368

Sequences

	Sequence		Length	Mass (Da)
	B1VGB6 [UniParc]. Last modified May 20, 2008. Version 1. Checksum: 0AA4BC5C257B5C99	FASTA	592	65,806
	10 20 30 40 50 60 MITRMSSLFL HTLRDDPADA ELASHKLLVR AGYIRRVAPG VYSWLPMGLR VLRKVENVVR 70 80 90 100 110 120 REMDAIGGQE LMFPALLPKE PYEVTRRWDE YGPELFRLKD RKEADYLLGP THEELFTWLV 130 140 150 160 170 180 KGELNSYKDF PKVLYQIQTK YRDEARPRAG ILRGREFVMK DSYSFNMTDE GLDESYRLHR 190 200 210 220 230 240 KAYQRIFDAL GIEYVICYAT SGAMGGSASE EFLAVAADGE DTFVRSTESD YAANVEAVVS 250 260 270 280 290 300 IPPAEESVED KPEAVSHESP GADTIETLVE WANEAGLTVD GAPVTAKDTL KCIVCKVTRP 310 320 330 340 350 360 GEDEDGNPYA PELTGVLVPG DREVDMKRLE ASLEPAVVEL ADDEDFKKYP FLVKGFVGPR 370 380 390 400 410 420 TFADNDLRML ADPRVVKGTA WITGADEKDR HYSNLVAGRD FTPDGYVEAA EVREGDPSPD 430 440 450 460 470 480 GKGVLTLSRG IEIGHIFQLG RKYTEAFDVQ ILDENGRRTV PTMGSYGIGV SRLLAVVAEQ 490 500 510 520 530 540 MHDEKGLRWP RSIAPYDVHV VIANKDEAAG EAARELAEAL SDAGLEVLFD DRPKVSPGVK 550 560 570 580 590 FKDSELLGMP QVVVVGRGFA EGKVELRDRL NDERSEIDYA DAVDVITKAA RA

References

[1]

"The lifestyle of Corynebacterium urealyticum derived from its complete genome sequence established by pyrosequencing." Tauch A., Trost E., Tilker A., Ludewig U., Schneiker S., Goesmann A., Arnold W., Bekel T., Brinkrolf K., Brune I., Goetker S., Kalinowski J., Kamp P.-B., Lobo F.P., Viehoever P., Weisshaar B., Soriano F., Droege M., Puehler A. J. Biotechnol. 136:11-21(2008) [PubMed: 18367281] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 43042 / DSM 7109.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AM942444 Genomic DNA. Translation: CAQ04805.1.
RefSeq	YP_001800239.1. NC_010545.1.
3D structure databases
ProteinModelPortal	B1VGB6.
ModBase	Search...
Protein-protein interaction databases
STRING	B1VGB6.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6185496.
GenomeReviews	Gene locus cu0845 in contig AM942444_GR.
KEGG	cur:cur_0845.
PATRIC	21521420. VBICorUre58120_0846.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG403504.
OMA	IQPAELW.
ProtClustDB	PRK09194.
Family and domain databases
HAMAP	MF_01569. Pro_tRNA_synth_type1. [Tree]
InterPro	IPR002314. aa-tRNA-synt_IIb_cons-dom. IPR006195. aa-tRNA-synth_II. IPR004154. Anticodon-bd. IPR002316. Pro-tRNA-synth_IIa. IPR004500. Pro-tRNA-synth_IIa_bac-type. IPR023717. Pro-tRNA-Synthase_IIa_type1. IPR007214. YbaK/aa-tRNA-synth-assoc-dom. [Graphical view]
Gene3D	G3DSA:3.40.50.800. Anticodon_bd. 1 hit. G3DSA:3.90.960.10. YbaK/aa-tRNA-synth-assoc-reg. 1 hit.
KO	K01881.
PANTHER	PTHR11451:SF3. PTHR11451:SF3. 1 hit.
Pfam	PF03129. HGTP_anticodon. 1 hit. PF00587. tRNA-synt_2b. 1 hit. PF04073. YbaK. 1 hit. [Graphical view]
PRINTS	PR01046. TRNASYNTHPRO.
SUPFAM	SSF52954. Anticodon_bd. 1 hit. SSF55826. YbaK/aa-tRNA-synth-assoc-reg. 1 hit.
TIGRFAMs	TIGR00409. ProS_fam_II. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYP_CORU7

Accession

Primary (citable) accession number: B1VGB6

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	May 20, 2008
Last modified:	January 25, 2012
This is version 31 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program