B1VG21 (B1VG21_CORU7) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
Dihydroxy-acid dehydratase HAMAP MF_00012
Short name=DAD HAMAP MF_00012
EC=4.2.1.9 HAMAP MF_00012

Gene names

Name:	ilvD HAMAP MF_00012 EMBL CAQ04710.1
Ordered Locus Names:	cu0750

Organism

Corynebacterium urealyticum (strain ATCC 43042 / DSM 7109) [Complete proteome] [HAMAP]

Taxonomic identifier

504474 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Corynebacteriaceae › Corynebacterium

Protein attributes

Sequence length	625 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	2,3-dihydroxy-3-methylbutanoate = 3-methyl-2-oxobutanoate + H2O. HAMAP MF_00012
Cofactor	Binds 1 4Fe-4S cluster By similarity. HAMAP MF_00012
Pathway	Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 3/4. HAMAP MF_00012 Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 3/4. HAMAP MF_00012
Sequence similarities	Belongs to the IlvD/Edd family. HAMAP MF_00012

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Branched-chain amino acid biosynthesis HAMAP MF_00012
Ligand	4Fe-4S HAMAP MF_00012 Iron Iron-sulfur Metal-binding
Molecular function	Lyase HAMAP MF_00012 EMBL CAQ04710.1
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	isoleucine biosynthetic process Inferred from electronic annotation. Source: HAMAP valine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW dihydroxy-acid dehydratase activity Inferred from electronic annotation. Source: HAMAP metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Sites
	Metal binding	134	1	Iron-sulfur (4Fe-4S) By similarity HAMAP MF_00012
	Metal binding	209	1	Iron-sulfur (4Fe-4S) By similarity HAMAP MF_00012

Sequences

	Sequence		Length	Mass (Da)
	B1VG21 [UniParc]. Last modified May 20, 2008. Version 1. Checksum: F82F899271CB04A0	FASTA	625	66,400
	10 20 30 40 50 60 MTSQDNTTSF GASIPLRSDV TTQGRNAAGA RALWRATGMT DDDFHKPIIA IANSYTQFVP 70 80 90 100 110 120 GHVHLKDVGD IVAEAIEEAG GVAREFNTIA VDDGIAMGHA GMLYSLPSRE IISDSVEYMV 130 140 150 160 170 180 NGHAADALVC ISNCDKITPG MLNAAMRLNI PVVFVSGGPM EAGKAVAVDG VVQAPTDLIT 190 200 210 220 230 240 AISASASDRV DAQGLLDVES AACPTCGSCS GMFTANSMNC LTEALGLSLP GNGSTLATHE 250 260 270 280 290 300 YRRRLFTQAG HTIVDLCRRY YGEGDESVLP RNIATKHAFQ NAMALDMAMG GSTNTVLHIL 310 320 330 340 350 360 AAAQEGEVDF TLQDIDDLSR EVPCLSKVSP NSDFHMEDVH RAGGIPAILG ELWRGGKLNE 370 380 390 400 410 420 DVHTVHANSL SEWLEAWDIR AENPSDAAID LFHAAPGGAR TTEAFSTENR WSSLDTDAAG 430 440 450 460 470 480 GCIRDVEHAY TADGGLVVLR GNLAEDGAII KAAGIDEELW RFEGKALVVE SQEDAVRVIL 490 500 510 520 530 540 DKKVKPGDCI VVRYEGPAGG PGMQEMLHPT AFLKGSGLGR ECALITDGRF SGGSSGISVG 550 560 570 580 590 600 HISPEAAHGG LIGLVEDGDP ILIDVHDRTL ELQISEEEIA RRREQMDASD TPWQPTTRKR 610 620 RVTKALRAYA AMATSADKGA VREVK

References

[1]

"The lifestyle of Corynebacterium urealyticum derived from its complete genome sequence established by pyrosequencing." Tauch A., Trost E., Tilker A., Ludewig U., Schneiker S., Goesmann A., Arnold W., Bekel T., Brinkrolf K., Brune I., Goetker S., Kalinowski J., Kamp P.-B., Lobo F.P., Viehoever P., Weisshaar B., Soriano F., Droege M., Puehler A. J. Biotechnol. 136:11-21(2008) [PubMed: 18367281] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 43042 / DSM 7109.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AM942444 Genomic DNA. Translation: CAQ04710.1.
RefSeq	YP_001800144.1. NC_010545.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	B1VG21.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6185300.
GenomeReviews	Gene locus cu0750 in contig AM942444_GR.
KEGG	cur:cur_0750.
PATRIC	21521222. VBICorUre58120_0750.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG671001.
OMA	SQDCRWP.
ProtClustDB	PRK12448.
Family and domain databases
HAMAP	MF_00012. IlvD. [Tree]
InterPro	IPR015928. Aconitase/3IPM_dehydase_swvl. IPR004404. DihydroxyA_deHydtase. IPR000581. DiOHA_6PGluconate_deHydtase. IPR020558. DiOHA_6PGluconate_deHydtase_CS. [Graphical view]
KO	K01687.
PANTHER	PTHR21000. ILVD_EDD_family. 1 hit.
Pfam	PF00920. ILVD_EDD. 1 hit. [Graphical view]
SUPFAM	SSF52016. Aconitase/3IPM_dehydase_swvl. 1 hit.
TIGRFAMs	TIGR00110. IlvD. 1 hit.
PROSITE	PS00886. ILVD_EDD_1. 1 hit. PS00887. ILVD_EDD_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

B1VG21_CORU7

Accession

Primary (citable) accession number: B1VG21

Entry history

Integrated into UniProtKB/TrEMBL:	May 20, 2008
Last sequence update:	May 20, 2008
Last modified:	January 25, 2012
This is version 30 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)