B1VES1 (RPOB_CORU7) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
DNA-directed RNA polymerase subunit beta
Short name=RNAP subunit beta
EC=2.7.7.6

Alternative name(s):
RNA polymerase subunit beta
Transcriptase subunit beta

Gene names

Name:	rpoB
Ordered Locus Names:	cu0300

Organism

Corynebacterium urealyticum (strain ATCC 43042 / DSM 7109) [Complete proteome] [HAMAP]

Taxonomic identifier

504474 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Corynebacteriaceae › Corynebacterium

Protein attributes

Sequence length	1170 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates By similarity. HAMAP MF_01321
Catalytic activity	Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1). HAMAP MF_01321
Subunit structure	The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta' and 1 omega subunit. When a sigma factor is associated with the core the holoenzyme is formed, which can initiate transcription By similarity.
Sequence similarities	Belongs to the RNA polymerase beta chain family.

Ontologies

Keywords
Biological process	Transcription
Cellular component	DNA-directed RNA polymerase
Molecular function	Nucleotidyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Molecular function	DNA binding Inferred from electronic annotation. Source: InterPro DNA-directed RNA polymerase activity Inferred from electronic annotation. Source: UniProtKB-KW ribonucleoside binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1170	1170	DNA-directed RNA polymerase subunit beta HAMAP MF_01321		PRO_1000141681

Sequences

	Sequence		Length	Mass (Da)
	B1VES1 [UniParc]. Last modified May 20, 2008. Version 1. Checksum: 9FA795063E15194C	FASTA	1,170	129,574
	10 20 30 40 50 60 MLEGPILAVS RQTSSVAGIP GASTRYSFAK IDAPIEVPGL LDLQRESFAW LVGAPEWRAR 70 80 90 100 110 120 MQAEAGEGVR VTSGLEDILE ELSPIEDYSE NMSLTLSEPR FDDMKTSIDE AKEKDINYAA 130 140 150 160 170 180 PLYVTAEFTN AQSGEIKSQT VFIGDFPMMT DKGTFIINGT ERVVVSQLVR SPGVYFDESI 190 200 210 220 230 240 DTSTERPLHG VKVIPSRGAW LEFDVDKRDT VGVRIDRKRR QPVTVLLKAL GLTTQEITDR 250 260 270 280 290 300 FGFSEIMMST LEKDGVENTD EALLEIYRKQ RPGESPTRDS AQALLENSFF RPKRYDLAKV 310 320 330 340 350 360 GRYKVNRKLG LGGDTDGTMT LTEEDILTTI EYLVRLHAGE RTMTSPEGVE IPIEVDDIDH 370 380 390 400 410 420 FGNRRLRTVG ELIQNQVRVG LSRMERVVRE RMTTQDAESI TPTSLINVRP VSAAIREFFG 430 440 450 460 470 480 TSQLSQFMDQ NNSLSGLTHK RRLNALGPGG LSRERAGLEV RDVHPSHYGR MCPIETPEGP 490 500 510 520 530 540 NIGLIGSLSS YARVNPFGFI ETPYRRVVDG QITDEVEYFT ADEEDRHVIA QANTPFDADM 550 560 570 580 590 600 KFTEDQIEVR LRGGDVEVVP ASQVDYMDVS PRQMVSVATA MIPFLEHDDA NRALMGANMQ 610 620 630 640 650 660 RQAVPLLRAE APYVGTGIEQ RAAYDAGDLI IAPKAGVVEY VSADYITIMD DEGIRDTFML 670 680 690 700 710 720 RKFERTNQGT SYNQKPLVNQ GDRVEAGQVI ADGPGTDNGE MALGKNLLVA FMPWEGHNYE 730 740 750 760 770 780 DAIILSQRMV EEDVLTSIHI EEYEIDARDT KLGPEEITRD IPNVGEDVLA DLDERGIVRI 790 800 810 820 830 840 GADVRDGDIL VGKVTPKGET ELTPEERLLR AIFGEKAREV RDTSMKVPHG ETGKVIGVRV 850 860 870 880 890 900 FSREDDDDLA AGVNEMVRVY VAQKRKIQDG DKLAGRHGNK GVVGKILPQE DMPFLPDGTP 910 920 930 940 950 960 VDIILNTHGV PRRMNIGQVL EVHLGWLAKA GWQVDTNSDD PKIKAMLETL PEDLYDVPAD 970 980 990 1000 1010 1020 SLTSTPVFDG ASNAELSGLL RSSRPDRDGI RLVDDFGKAQ LIDGRTGEPY EHPISVGYMY 1030 1040 1050 1060 1070 1080 MLKLHHLVDE KIHARSTGPY SMITQQPLGG KAQFGGQRFG EMEVWAMQAY GAAYTLQELL 1090 1100 1110 1120 1130 1140 TIKSDDVVGR VKVYEAIVKG ENIPDPGIPE SFKVLLKELQ SLCLNVEVLA ADGTPMELSS 1150 1160 1170 DDDDELESAN AALGINLQRD ERPDADIDVG

References

[1]

"The lifestyle of Corynebacterium urealyticum derived from its complete genome sequence established by pyrosequencing." Tauch A., Trost E., Tilker A., Ludewig U., Schneiker S., Goesmann A., Arnold W., Bekel T., Brinkrolf K., Brune I., Goetker S., Kalinowski J., Kamp P.-B., Lobo F.P., Viehoever P., Weisshaar B., Soriano F., Droege M., Puehler A. J. Biotechnol. 136:11-21(2008) [PubMed: 18367281] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 43042 / DSM 7109.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AM942444 Genomic DNA. Translation: CAQ04260.1.
RefSeq	YP_001799694.1. NC_010545.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	B1VES1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6186306.
GenomeReviews	Gene locus cu0300 in contig AM942444_GR.
KEGG	cur:cur_0300.
PATRIC	21520308. VBICorUre58120_0297.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG285547.
OMA	QRDLTYA.
ProtClustDB	PRK00405.
Family and domain databases
HAMAP	MF_01321. RNApol_bact_RpoB. [Tree]
InterPro	IPR010243. DNA-dir_RNA_pol_bsu_bac-type. IPR019462. DNA-dir_RNA_pol_bsu_external_1. IPR015712. DNA-dir_RNA_pol_su2. IPR007120. DNA-dir_RNA_pol_su2_6. IPR007121. RNA_pol_bsu_CS. IPR007644. RNA_pol_bsu_protrusion. IPR007642. RNA_pol_Rpb2_2. IPR007645. RNA_pol_Rpb2_3. IPR007641. RNA_pol_Rpb2_7. IPR014724. RNA_pol_RPB2_OB-fold. [Graphical view]
Gene3D	G3DSA:2.30.150.10. G3DSA:2.30.150.10. 1 hit. G3DSA:2.40.270.10. G3DSA:2.40.270.10. 2 hits. G3DSA:2.40.50.150. Ribosomal_L2. 1 hit.
KO	K03043.
PANTHER	PTHR20856:SF3. PTHR20856:SF3. 1 hit. PTHR20856. RNA_pol_I_sub2. 1 hit.
Pfam	PF04563. RNA_pol_Rpb2_1. 1 hit. PF04561. RNA_pol_Rpb2_2. 1 hit. PF04565. RNA_pol_Rpb2_3. 1 hit. PF10385. RNA_pol_Rpb2_45. 1 hit. PF00562. RNA_pol_Rpb2_6. 1 hit. PF04560. RNA_pol_Rpb2_7. 1 hit. [Graphical view]
TIGRFAMs	TIGR02013. RpoB. 1 hit.
PROSITE	PS01166. RNA_POL_BETA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RPOB_CORU7

Accession

Primary (citable) accession number: B1VES1

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	May 20, 2008
Last modified:	January 25, 2012
This is version 31 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program