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Protein

tRNA (guanine-N(7)-)-methyltransferase

Gene

trmB

Organism
Corynebacterium urealyticum (strain ATCC 43042 / DSM 7109)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + guanine(46) in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine(46) in tRNA.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei102 – 1021S-adenosyl-L-methionineUniRule annotation
Binding sitei127 – 1271S-adenosyl-L-methionineUniRule annotation
Binding sitei154 – 1541S-adenosyl-L-methionineUniRule annotation
Active sitei177 – 1771By similarity
Binding sitei177 – 1771S-adenosyl-L-methionineUniRule annotation
Binding sitei181 – 1811SubstrateUniRule annotation
Binding sitei213 – 2131SubstrateUniRule annotation

GO - Molecular functioni

  1. tRNA (guanine-N7-)-methyltransferase activity Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciCURE504474:GJ8Y-176-MONOMER.
UniPathwayiUPA00989.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (guanine-N(7)-)-methyltransferaseUniRule annotation (EC:2.1.1.33UniRule annotation)
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferaseUniRule annotation
tRNA(m7G46)-methyltransferaseUniRule annotation
Gene namesi
Name:trmBUniRule annotation
Ordered Locus Names:cu0170
OrganismiCorynebacterium urealyticum (strain ATCC 43042 / DSM 7109)
Taxonomic identifieri504474 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeCorynebacteriaceaeCorynebacterium
ProteomesiUP000001727: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 282282tRNA (guanine-N(7)-)-methyltransferasePRO_1000149654Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi504474.cur_0170.

Structurei

3D structure databases

ProteinModelPortaliB1VEE1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni261 – 2644Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0220.
KOiK03439.
OMAiNDGRDYP.
OrthoDBiEOG6K6VBC.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_01057. tRNA_methyltr_TrmB.
InterProiIPR029063. SAM-dependent_MTases.
IPR003358. tRNA_(Gua-N-7)_MeTrfase_Trmb.
[Graphical view]
PfamiPF02390. Methyltransf_4. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00091. TIGR00091. 1 hit.
PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B1VEE1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTSSNNSPQD DHQQALSEEN SAPATEVTGG TGRRPLQTVF NDGRDYPRLS
60 70 80 90 100
SFAFRRGTLT DNQEATWEAN WPRLGKELDE QVNEQYIDLD EWFGRHADTI
110 120 130 140 150
VEIGSGTGTS TAAMAPLETD KNIVAVELYR PGLAKLVGAV SREGIENIRM
160 170 180 190 200
IKGDGVEVLQ RMFRPESLAG VRIFFPDPWP KARHHKRRIV QTGTLHLIAS
210 220 230 240 250
RLRPGGILHI ATDHAEYAEW IDELVNAEPQ LEYIGWPEEL ASGADDGRGH
260 270 280
LGQLVDRQLL TKFEDKGLRK EHTIKEYLWT KK
Length:282
Mass (Da):31,749
Last modified:May 20, 2008 - v1
Checksum:i999421B764F4F223
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM942444 Genomic DNA. Translation: CAQ04130.1.
RefSeqiYP_001799564.1. NC_010545.1.

Genome annotation databases

EnsemblBacteriaiCAQ04130; CAQ04130; cu0170.
GeneIDi6186263.
KEGGicur:cur_0170.
PATRICi21520042. VBICorUre58120_0168.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM942444 Genomic DNA. Translation: CAQ04130.1.
RefSeqiYP_001799564.1. NC_010545.1.

3D structure databases

ProteinModelPortaliB1VEE1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi504474.cur_0170.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAQ04130; CAQ04130; cu0170.
GeneIDi6186263.
KEGGicur:cur_0170.
PATRICi21520042. VBICorUre58120_0168.

Phylogenomic databases

eggNOGiCOG0220.
KOiK03439.
OMAiNDGRDYP.
OrthoDBiEOG6K6VBC.

Enzyme and pathway databases

UniPathwayiUPA00989.
BioCyciCURE504474:GJ8Y-176-MONOMER.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_01057. tRNA_methyltr_TrmB.
InterProiIPR029063. SAM-dependent_MTases.
IPR003358. tRNA_(Gua-N-7)_MeTrfase_Trmb.
[Graphical view]
PfamiPF02390. Methyltransf_4. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00091. TIGR00091. 1 hit.
PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The lifestyle of Corynebacterium urealyticum derived from its complete genome sequence established by pyrosequencing."
    Tauch A., Trost E., Tilker A., Ludewig U., Schneiker S., Goesmann A., Arnold W., Bekel T., Brinkrolf K., Brune I., Goetker S., Kalinowski J., Kamp P.-B., Lobo F.P., Viehoever P., Weisshaar B., Soriano F., Droege M., Puehler A.
    J. Biotechnol. 136:11-21(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 43042 / DSM 7109.

Entry informationi

Entry nameiTRMB_CORU7
AccessioniPrimary (citable) accession number: B1VEE1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: May 20, 2008
Last modified: January 7, 2015
This is version 47 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.