B1VDC4 (DAPA_CORU7) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Dihydrodipicolinate synthase
Short name=DHDPS
EC=4.2.1.52

Gene names

Name:	dapA
Ordered Locus Names:	cu0862

Organism

Corynebacterium urealyticum (strain ATCC 43042 / DSM 7109) [Complete proteome] [HAMAP]

Taxonomic identifier

504474 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Corynebacteriaceae › Corynebacterium

Protein attributes

Sequence length	300 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	L-aspartate 4-semialdehyde + pyruvate = dihydrodipicolinate + 2 H2O. HAMAP MF_00418
Pathway	Amino-acid biosynthesis; L-lysine biosynthesis via DAP pathway; (S)-tetrahydrodipicolinate from L-aspartate: step 3/4. HAMAP MF_00418
Subunit structure	Homotetramer By similarity. HAMAP MF_00418
Subcellular location	Cytoplasm By similarity HAMAP MF_00418.
Sequence similarities	Belongs to the DHDPS family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Diaminopimelate biosynthesis Lysine biosynthesis
Cellular component	Cytoplasm
Ligand	Schiff base
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	diaminopimelate biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	dihydrodipicolinate synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 300	300	Dihydrodipicolinate synthase HAMAP MF_00418		PRO_1000124025
Regions
	Region	60 – 61	2	Pyruvate binding By similarity
Sites
	Active site	173	1	Schiff-base intermediate with substrate By similarity
	Binding site	118	1	Pyruvate By similarity
	Site	145	1	Involved in proton transfer during cleavage By similarity

Sequences

	Sequence		Length	Mass (Da)
	B1VDC4 [UniParc]. Last modified May 20, 2008. Version 1. Checksum: 773A226EBFCFDB39	FASTA	300	31,710
	10 20 30 40 50 60 MSTGNAAIRG AEHFGTVSVA MVTPFHKDGT IDLKAGVELA GYLVDKGCDS LVLAGTTGES 70 80 90 100 110 120 PTTTNEEKIN LLKAVRAELG DSVKLIAGTG TNNTASTIEL SRLSQDAGAD SLLVVTPYYS 130 140 150 160 170 180 RPSQEGLFQH FTAVADSVDL PICLYDIPSR SVVPIELDTI QRLSEHENIQ AVKDAKGKLP 190 200 210 220 230 240 EALQLLQNTD LAWYSGDDPL NLPFLSVGAT GFISVIGHLA ADRLRALRDA YDQGDLAGAQ 250 260 270 280 290 300 QIAASLAPLE RAQGRLGGVT MVKAALKLRG KDVGAPRLPI LEVDPAELDQ LEQDLQDAGV

References

[1]

"The lifestyle of Corynebacterium urealyticum derived from its complete genome sequence established by pyrosequencing." Tauch A., Trost E., Tilker A., Ludewig U., Schneiker S., Goesmann A., Arnold W., Bekel T., Brinkrolf K., Brune I., Goetker S., Kalinowski J., Kamp P.-B., Lobo F.P., Viehoever P., Weisshaar B., Soriano F., Droege M., Puehler A. J. Biotechnol. 136:11-21(2008) [PubMed: 18367281] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 43042 / DSM 7109.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AM942444 Genomic DNA. Translation: CAQ04822.1.
RefSeq	YP_001800256.1. NC_010545.1.
3D structure databases
ProteinModelPortal	B1VDC4.
SMR	B1VDC4. Positions 11-300.
ModBase	Search...
Protein-protein interaction databases
STRING	B1VDC4.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6186068.
GenomeReviews	Gene locus cu0862 in contig AM942444_GR.
KEGG	cur:cur_0862.
PATRIC	21521452. VBICorUre58120_0862.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG358848.
OMA	HGCISVV.
ProtClustDB	PRK03170.
Family and domain databases
HAMAP	MF_00418. DapA. [Tree]
InterPro	IPR013785. Aldolase_TIM. IPR002220. Dihydrodipicolinate_synth-like. IPR020625. Dihydrodipicolinate_synth_AS. IPR020624. Dihydrodipicolinate_synth_CS. IPR005263. Dihydrodipicolinate_synth_DapA. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
KO	K01714.
PANTHER	PTHR12128. DHDPS. 1 hit.
Pfam	PF00701. DHDPS. 1 hit. [Graphical view]
PIRSF	PIRSF001365. DHDPS. 1 hit.
PRINTS	PR00146. DHPICSNTHASE.
TIGRFAMs	TIGR00674. DapA. 1 hit.
PROSITE	PS00665. DHDPS_1. 1 hit. PS00666. DHDPS_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DAPA_CORU7

Accession

Primary (citable) accession number: B1VDC4

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	May 20, 2008
Last modified:	January 25, 2012
This is version 29 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program