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Protein

Polyribonucleotide nucleotidyltransferase

Gene

pnp

Organism
Phytoplasma australiense
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Involved in mRNA degradation. Catalyzes the phosphorolysis of single-stranded polyribonucleotides processively in the 3'- to 5'-direction.UniRule annotation

Catalytic activityi

RNA(n+1) + phosphate = RNA(n) + a nucleoside diphosphate.UniRule annotation

Cofactori

Mg2+UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi501 – 5011MagnesiumUniRule annotation
Metal bindingi507 – 5071MagnesiumUniRule annotation

GO - Molecular functioni

  1. 3'-5'-exoribonuclease activity Source: InterPro
  2. magnesium ion binding Source: UniProtKB-HAMAP
  3. polyribonucleotide nucleotidyltransferase activity Source: UniProtKB-HAMAP
  4. RNA binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. mRNA catabolic process Source: UniProtKB-HAMAP
  2. RNA processing Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, Transferase

Keywords - Ligandi

Magnesium, Metal-binding, RNA-binding

Enzyme and pathway databases

BioCyciPAUS59748:GJ8P-684-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Polyribonucleotide nucleotidyltransferaseUniRule annotation (EC:2.7.7.8UniRule annotation)
Alternative name(s):
Polynucleotide phosphorylaseUniRule annotation
Short name:
PNPaseUniRule annotation
Gene namesi
Name:pnpUniRule annotation
Ordered Locus Names:PA0674
OrganismiPhytoplasma australiense
Taxonomic identifieri59748 [NCBI]
Taxonomic lineageiBacteriaTenericutesMollicutesAcholeplasmatalesAcholeplasmataceaeCandidatus Phytoplasma16SrXII (Stolbur group)
ProteomesiUP000008323: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 710710Polyribonucleotide nucleotidyltransferasePRO_1000192481Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi59748.PAa_0674.

Structurei

3D structure databases

ProteinModelPortaliB1VAN5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini568 – 62861KHUniRule annotationAdd
BLAST
Domaini638 – 71073S1 motifUniRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the polyribonucleotide nucleotidyltransferase family.UniRule annotation
Contains 1 KH domain.UniRule annotation
Contains 1 S1 motif domain.UniRule annotation

Phylogenomic databases

eggNOGiCOG1185.
KOiK00962.
OMAiRFMFHYN.
OrthoDBiEOG6WT8CC.

Family and domain databases

Gene3Di1.10.10.400. 1 hit.
2.40.50.140. 1 hit.
3.30.1370.10. 1 hit.
3.30.230.70. 2 hits.
HAMAPiMF_01595. PNPase.
InterProiIPR001247. ExoRNase_PH_dom1.
IPR015847. ExoRNase_PH_dom2.
IPR004087. KH_dom.
IPR004088. KH_dom_type_1.
IPR012340. NA-bd_OB-fold.
IPR012162. PNPase.
IPR027408. PNPase/RNase_PH_dom.
IPR015848. PNPase_PH_RNA-bd_bac/org-type.
IPR003029. Rbsml_prot_S1_RNA-bd_dom.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR022967. S1_dom.
[Graphical view]
PANTHERiPTHR11252. PTHR11252. 1 hit.
PfamiPF00013. KH_1. 1 hit.
PF01138. RNase_PH. 2 hits.
PF03725. RNase_PH_C. 2 hits.
[Graphical view]
PIRSFiPIRSF005499. PNPase. 1 hit.
SMARTiSM00322. KH. 1 hit.
SM00316. S1. 1 hit.
[Graphical view]
SUPFAMiSSF46915. SSF46915. 1 hit.
SSF50249. SSF50249. 1 hit.
SSF54211. SSF54211. 2 hits.
SSF54791. SSF54791. 1 hit.
SSF55666. SSF55666. 2 hits.
TIGRFAMsiTIGR03591. polynuc_phos. 1 hit.
PROSITEiPS50084. KH_TYPE_1. 1 hit.
PS50126. S1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B1VAN5-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MLKQVFETTD LKNSFQVEIG TYARNVDPSV LIRFQDTVVL TTTVFSNKKN
60 70 80 90 100
NFDFLPLTVI YQEKFYAAGK IPGSFLRREG RSTDHEILSS RLIDRSLRPL
110 120 130 140 150
FPKEFRQEIQ VINTVLSSNP DFKSEIASIL GSSLSLLISE IPFFEPVAGV
160 170 180 190 200
YVAYIQNQFV INPNAQQLTN SPLHLLVAGT KKNVVMIEAH ASEVSEEMFL
210 220 230 240 250
EAIVFAHEYI KKLCLFQEDV QKKVGQTKKL IDLDAELQSL EKEFNEQYHE
260 270 280 290 300
QTRTLVANVF EQDQKNDLQV FKKKILAKAQ QKAFVKTIDQ ITFFDVEEQK
310 320 330 340 350
NYLLLIENLF QKLFNQEMRN YIIKNKKRPD KRTLEEVRNL DSQIDLLPRP
360 370 380 390 400
HGSALFTRGQ TQSLAVVTLG TLSESKIIDD LSGESNKRFM LHYNFPPFAV
410 420 430 440 450
GSIGRYAAPS RREIGHGNLA EKAILPLLPE ENDFPYAIRV VSEILESNGS
460 470 480 490 500
SSQATVCATS MSLMAAGVPL KRAVSGIAMG LFMDSKTNEY VILSDIQGLE
510 520 530 540 550
DHIGDMDLKI AGSDKGITAL QMDLKIEGIS QAILKQAFFQ AKKGRLHILE
560 570 580 590 600
HMNKTIASPR KEMSQYAPKV QMFQIKPEKI RDVIGSAGKI INQIIENHDG
610 620 630 640 650
VKIDIEQDGR IFVMHSNLET VKQAILFIKN LIQDAEVNSI YHAHISRFLN
660 670 680 690 700
DKAGNILGAF AQVSPSIEGL IRFSKAKKEN DVVKIGDKVL VKCVKINERG
710
RIDFVLISKK
Length:710
Mass (Da):80,156
Last modified:May 20, 2008 - v1
Checksum:i12161A596AB8036E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM422018 Genomic DNA. Translation: CAM12008.1.
RefSeqiWP_012359136.1. NC_010544.1.
YP_001799249.1. NC_010544.1.

Genome annotation databases

EnsemblBacteriaiCAM12008; CAM12008; EBG00000298957.
GeneIDi6799005.
KEGGipal:PAa_0674.
PATRICi31993750. VBICanPhy111091_0737.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM422018 Genomic DNA. Translation: CAM12008.1.
RefSeqiWP_012359136.1. NC_010544.1.
YP_001799249.1. NC_010544.1.

3D structure databases

ProteinModelPortaliB1VAN5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi59748.PAa_0674.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAM12008; CAM12008; EBG00000298957.
GeneIDi6799005.
KEGGipal:PAa_0674.
PATRICi31993750. VBICanPhy111091_0737.

Phylogenomic databases

eggNOGiCOG1185.
KOiK00962.
OMAiRFMFHYN.
OrthoDBiEOG6WT8CC.

Enzyme and pathway databases

BioCyciPAUS59748:GJ8P-684-MONOMER.

Family and domain databases

Gene3Di1.10.10.400. 1 hit.
2.40.50.140. 1 hit.
3.30.1370.10. 1 hit.
3.30.230.70. 2 hits.
HAMAPiMF_01595. PNPase.
InterProiIPR001247. ExoRNase_PH_dom1.
IPR015847. ExoRNase_PH_dom2.
IPR004087. KH_dom.
IPR004088. KH_dom_type_1.
IPR012340. NA-bd_OB-fold.
IPR012162. PNPase.
IPR027408. PNPase/RNase_PH_dom.
IPR015848. PNPase_PH_RNA-bd_bac/org-type.
IPR003029. Rbsml_prot_S1_RNA-bd_dom.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR022967. S1_dom.
[Graphical view]
PANTHERiPTHR11252. PTHR11252. 1 hit.
PfamiPF00013. KH_1. 1 hit.
PF01138. RNase_PH. 2 hits.
PF03725. RNase_PH_C. 2 hits.
[Graphical view]
PIRSFiPIRSF005499. PNPase. 1 hit.
SMARTiSM00322. KH. 1 hit.
SM00316. S1. 1 hit.
[Graphical view]
SUPFAMiSSF46915. SSF46915. 1 hit.
SSF50249. SSF50249. 1 hit.
SSF54211. SSF54211. 2 hits.
SSF54791. SSF54791. 1 hit.
SSF55666. SSF55666. 2 hits.
TIGRFAMsiTIGR03591. polynuc_phos. 1 hit.
PROSITEiPS50084. KH_TYPE_1. 1 hit.
PS50126. S1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Comparative genome analysis of 'Candidatus Phytoplasma australiense' (subgroup tuf-Australia I; rp-A) and 'Ca. Phytoplasma asteris' strains OY-M and AY-WB."
    Tran-Nguyen L.T., Kube M., Schneider B., Reinhardt R., Gibb K.S.
    J. Bacteriol. 190:3979-3991(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Entry informationi

Entry nameiPNP_PHYAS
AccessioniPrimary (citable) accession number: B1VAN5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: May 20, 2008
Last modified: January 7, 2015
This is version 48 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.