Reviewed, UniProtKB/Swiss-Prot B1V8K7 (GLAA_STRGU)

Names and origin

Protein names

Recommended name:
    Alpha-1,3-galactosidase A
    EC=3.2.1.n1
Alternative name(s):
    Exo-alpha-galactosidase A
    EC=3.2.1.22
    SgGal110A

Gene names

Name:	glaA
Synonyms:	gla

Organism

Streptomyces griseoplanus

Taxonomic identifier

66896 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Streptomycineae › Streptomycetaceae › Streptomyces

Protein attributes

Sequence length	727 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Alpha-galactosidase that specifically removes branched alpha-1,3-linked galactose residues present in blood group B antigens. Has no activity toward linear alpha-1,3-linked galactose residues.
Catalytic activity	Hydrolysis of terminal, non-reducing branched alpha-1,3-linked galactose residues. Ref.1 Ref.2 Hydrolysis of terminal, non-reducing alpha-D-galactose residues in alpha-D-galactosides, including galactose oligosaccharides, galactomannans and galactohydrolase. Ref.1 Ref.2
Biotechnological use	Specifically cleaves the branched blood group B antigen at neutral pH with low consumption of recombinant enzyme. It is therefore a good candidate to participate to the development of universal red blood cells by removing blood group B antigen.
Sequence similarities	Belongs to the glycosyl hydrolase 110 family. A subfamily. Contains 6 PbH1 repeats.

Ontologies

Keywords
Domain	Repeat
Molecular function	Glycosidase Hydrolase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	alpha-galactosidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 727	727	Alpha-1,3-galactosidase A		PRO_0000348460
Regions
	Repeat	336 – 358	23	PbH1 1
	Repeat	461 – 483	23	PbH1 2
	Repeat	484 – 506	23	PbH1 3
	Repeat	517 – 538	22	PbH1 4
	Repeat	551 – 572	22	PbH1 5
	Repeat	574 – 603	30	PbH1 6
Experimental info
	Sequence conflict	66	1	G → I AA sequence Ref.2
	Sequence conflict	76	1	T → G AA sequence Ref.2
	Sequence conflict	492	1	N → S AA sequence Ref.2
	Sequence conflict	496	1	G → D AA sequence Ref.2

Sequences

	Sequence		Length	Mass (Da)
	B1V8K7-1 [UniParc]. Last modified May 20, 2008. Version 1. Checksum: 059E250ED79FB764	FASTA	727	78,244
	10 20 30 40 50 60 MGTATAQPAL RPQTSTVIGG LHGAAVLDNT GRTVIDVTDF GADPSGKADS AAAVSAAMAH 70 80 90 100 110 120 AKTVGGPTTL HFPTGTYHIW PERTPKRELY VSNTVGSDQA FRTKNIGILV EDMRDVVVDG 130 140 150 160 170 180 GGSRIVNHGF QTVFAAIRSS DVRFTNFSQT WVAPKTVDIT VADAGVVSGQ AYRIIDIPET 190 200 210 220 230 240 YDYAVEGTSV RWNGERGPAT GQPYWTGTNS FDYSQVHDPA TNRTWRTSNP VFPERHEDHR 250 260 270 280 290 300 PRRRQVRITY GDSTAPGDRG YVYQMREVTR DTPGALFWES SRVTVDHLRL GYLHGFGIVG 310 320 330 340 350 360 QLSEDIGIDS VTFKADRGSG RVTSGFADHI QMSGVKGTVR ITNSVFDNPQ DDPINIHGTY 370 380 390 400 410 420 LQATAAERET LQLRYMHNET SGFPQFYPGD TIELVDKRTM LAAPGATAKV VSVTGPTGSG 430 440 450 460 470 480 VPAGTDPDTY LRTMTVVLDR TLPAAVLAAP GDYVAENTTY TPTVEITGNT FQAVPTRGIL 490 500 510 520 530 540 VTTRRPVRIE NNRFDGMSMA SIYISSDARS WYESGPVRNV TIRGNVFDRP ASPVIFFDPT 550 560 570 580 590 600 NQDFVAGQPV HRNVLIEDND FNLTGGTILS GRGVGGLTFR DNRVERYPHL RLTGPSRALR 610 620 630 640 650 660 VGDTTTVTTD APPPSHTSPL FTFDGADDIT LANNTYGNGF NKRVNTANMD VSEITVTADG 670 680 690 700 710 720 LALNADSISS APVAVSYSSS RPKVATVDSE GVVKALSGGT TSITARATIG GVRVTSNPVK VVVATER

References

[1]

"Identification of a GH110 subfamily of alpha1,3-galactosidases: novel enzymes for removal of the alpha3Gal xenotransplantation antigen." Liu Q.P., Yuan H., Bennett E.P., Levery S.B., Nudelman E., Spence J., Pietz G., Saunders K., White T., Olsson M.L., Henrissat B., Sulzenbacher G., Clausen H. J. Biol. Chem. 283:8545-8554(2008) [PubMed: 18227066] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], ENZYME ACTIVITY.

[2]

"Bacterial glycosidases for the production of universal red blood cells." Liu Q.P., Sulzenbacher G., Yuan H., Bennett E.P., Pietz G., Saunders K., Spence J., Nudelman E., Levery S.B., White T., Neveu J.M., Lane W.S., Bourne Y., Olsson M.L., Henrissat B., Clausen H. Nat. Biotechnol. 25:454-464(2007) [PubMed: 17401360] [Abstract] Cited for: PROTEIN SEQUENCE OF 34-77; 144-153; 273-280 AND 489-497, CATALYTIC ACTIVITY.

Web resources

Protein Spotlight

The juice of life - Issue 98 of October 2008

Cross-references

Sequence databases
EMBLGenBankDDBJ	AM259273 Genomic DNA. Translation: CAJ90659.1.
3D structure databases
ModBase	Search...
Family and domain databases
InterPro	IPR003343. Big_2. IPR006626. PbH1. [Graphical view]
Pfam	PF02368. Big_2. 1 hit. [Graphical view]
SMART	SM00710. PbH1. 6 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GLAA_STRGU

Accession

Primary (citable) accession number: B1V8K7

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 2, 2008
Last sequence update:	May 20, 2008
Last modified:	March 3, 2009
This is version 6 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)