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Reviewed, UniProtKB/Swiss-Prot B1V8K7 (GLAA_STRGU)

Last modified March 3, 2009. Version 6. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Web resources · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    Alpha-1,3-galactosidase A
    EC=3.2.1.n1
Alternative name(s):
    Exo-alpha-galactosidase A
    EC=3.2.1.22
    SgGal110A
Gene names
Name: glaA
Synonyms: gla
OrganismStreptomyces griseoplanus
Taxonomic identifier66896 [NCBI]
Taxonomic lineageBacteriaActinobacteriaActinobacteridaeActinomycetalesStreptomycineaeStreptomycetaceaeStreptomyces

Protein attributes

Sequence length727 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at protein level.

General annotation (Comments)

Function

Alpha-galactosidase that specifically removes branched alpha-1,3-linked galactose residues present in blood group B antigens. Has no activity toward linear alpha-1,3-linked galactose residues.

Catalytic activity

Hydrolysis of terminal, non-reducing branched alpha-1,3-linked galactose residues. Ref.1 Ref.2

Hydrolysis of terminal, non-reducing alpha-D-galactose residues in alpha-D-galactosides, including galactose oligosaccharides, galactomannans and galactohydrolase. Ref.1 Ref.2

Biotechnological use

Specifically cleaves the branched blood group B antigen at neutral pH with low consumption of recombinant enzyme. It is therefore a good candidate to participate to the development of universal red blood cells by removing blood group B antigen.

Sequence similarities

Belongs to the glycosyl hydrolase 110 family. A subfamily.

Contains 6 PbH1 repeats.

Ontologies

Keywords
   DomainRepeat
   Molecular functionGlycosidase
Hydrolase
   Technical termDirect protein sequencing
Gene Ontology (GO)
   Biological processmetabolic process

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular functionalpha-galactosidase activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 727727Alpha-1,3-galactosidase A
PRO_0000348460

Regions

Repeat336 – 35823PbH1 1
Repeat461 – 48323PbH1 2
Repeat484 – 50623PbH1 3
Repeat517 – 53822PbH1 4
Repeat551 – 57222PbH1 5
Repeat574 – 60330PbH1 6

Experimental info

Sequence conflict661G → I AA sequence Ref.2
Sequence conflict761T → G AA sequence Ref.2
Sequence conflict4921N → S AA sequence Ref.2
Sequence conflict4961G → D AA sequence Ref.2

Sequences

Sequence LengthMass (Da)Tools
B1V8K7-1 [UniParc].

Last modified May 20, 2008. Version 1.
Checksum: 059E250ED79FB764

FASTA72778,244
        10         20         30         40         50         60 
MGTATAQPAL RPQTSTVIGG LHGAAVLDNT GRTVIDVTDF GADPSGKADS AAAVSAAMAH 

        70         80         90        100        110        120 
AKTVGGPTTL HFPTGTYHIW PERTPKRELY VSNTVGSDQA FRTKNIGILV EDMRDVVVDG 

       130        140        150        160        170        180 
GGSRIVNHGF QTVFAAIRSS DVRFTNFSQT WVAPKTVDIT VADAGVVSGQ AYRIIDIPET 

       190        200        210        220        230        240 
YDYAVEGTSV RWNGERGPAT GQPYWTGTNS FDYSQVHDPA TNRTWRTSNP VFPERHEDHR 

       250        260        270        280        290        300 
PRRRQVRITY GDSTAPGDRG YVYQMREVTR DTPGALFWES SRVTVDHLRL GYLHGFGIVG 

       310        320        330        340        350        360 
QLSEDIGIDS VTFKADRGSG RVTSGFADHI QMSGVKGTVR ITNSVFDNPQ DDPINIHGTY 

       370        380        390        400        410        420 
LQATAAERET LQLRYMHNET SGFPQFYPGD TIELVDKRTM LAAPGATAKV VSVTGPTGSG 

       430        440        450        460        470        480 
VPAGTDPDTY LRTMTVVLDR TLPAAVLAAP GDYVAENTTY TPTVEITGNT FQAVPTRGIL 

       490        500        510        520        530        540 
VTTRRPVRIE NNRFDGMSMA SIYISSDARS WYESGPVRNV TIRGNVFDRP ASPVIFFDPT 

       550        560        570        580        590        600 
NQDFVAGQPV HRNVLIEDND FNLTGGTILS GRGVGGLTFR DNRVERYPHL RLTGPSRALR 

       610        620        630        640        650        660 
VGDTTTVTTD APPPSHTSPL FTFDGADDIT LANNTYGNGF NKRVNTANMD VSEITVTADG 

       670        680        690        700        710        720 
LALNADSISS APVAVSYSSS RPKVATVDSE GVVKALSGGT TSITARATIG GVRVTSNPVK 


VVVATER 

« Hide

References

[1]"Identification of a GH110 subfamily of alpha1,3-galactosidases: novel enzymes for removal of the alpha3Gal xenotransplantation antigen."
Liu Q.P., Yuan H., Bennett E.P., Levery S.B., Nudelman E., Spence J., Pietz G., Saunders K., White T., Olsson M.L., Henrissat B., Sulzenbacher G., Clausen H.
J. Biol. Chem. 283:8545-8554(2008) [PubMed: 18227066] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], ENZYME ACTIVITY.
[2]"Bacterial glycosidases for the production of universal red blood cells."
Liu Q.P., Sulzenbacher G., Yuan H., Bennett E.P., Pietz G., Saunders K., Spence J., Nudelman E., Levery S.B., White T., Neveu J.M., Lane W.S., Bourne Y., Olsson M.L., Henrissat B., Clausen H.
Nat. Biotechnol. 25:454-464(2007) [PubMed: 17401360] [Abstract]
Cited for: PROTEIN SEQUENCE OF 34-77; 144-153; 273-280 AND 489-497, CATALYTIC ACTIVITY.

Web resources

Protein Spotlight

The juice of life - Issue 98 of October 2008

Cross-references

Sequence databases

AM259273 Genomic DNA. Translation: CAJ90659.1.

3D structure databases

ModBaseSearch...

Family and domain databases

InterProIPR003343. Big_2.
IPR006626. PbH1.
[Graphical view]
PfamPF02368. Big_2. 1 hit.
[Graphical view]
SMARTSM00710. PbH1. 6 hits.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameGLAA_STRGU
AccessionPrimary (citable) accession number: B1V8K7
Entry history
Integrated into UniProtKB/Swiss-Prot: September 2, 2008
Last sequence update: May 20, 2008
Last modified: March 3, 2009
This is version 6 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

Relevant documents

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries

Protein Spotlight

Protein Spotlight articles and cited UniProtKB/Swiss-Prot entries

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Web resources · Cross-references · Entry information · Relevant documents