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B1V8K7

- GLAA_STRGU

UniProt

B1V8K7 - GLAA_STRGU

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Protein

Alpha-1,3-galactosidase A

Gene

glaA

Organism
Streptomyces griseoplanus
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

Alpha-galactosidase that specifically removes branched alpha-1,3-linked galactose residues present in blood group B antigens. Has no activity toward linear alpha-1,3-linked galactose residues.

Catalytic activityi

Hydrolysis of terminal, non-reducing branched (1->3)-alpha-D-galactosidic residues, producing free D-galactose.
Hydrolysis of terminal, non-reducing alpha-D-galactose residues in alpha-D-galactosides, including galactose oligosaccharides, galactomannans and galactolipids.

GO - Molecular functioni

  1. raffinose alpha-galactosidase activity Source: UniProtKB-EC
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Protein family/group databases

CAZyiGH110. Glycoside Hydrolase Family 110.

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-1,3-galactosidase A (EC:3.2.1.n1)
Alternative name(s):
Exo-alpha-galactosidase A (EC:3.2.1.22)
SgGal110A
Gene namesi
Name:glaA
Synonyms:gla
OrganismiStreptomyces griseoplanus
Taxonomic identifieri66896 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesStreptomycineaeStreptomycetaceaeStreptomyces

Pathology & Biotechi

Biotechnological usei

Specifically cleaves the branched blood group B antigen at neutral pH with low consumption of recombinant enzyme. It is therefore a good candidate to participate in the development of universal red blood cells by removing blood group B antigen.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 727727Alpha-1,3-galactosidase APRO_0000348460Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliB1V8K7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati336 – 35823PbH1 1Add
BLAST
Repeati461 – 48323PbH1 2Add
BLAST
Repeati484 – 50623PbH1 3Add
BLAST
Repeati517 – 53822PbH1 4Add
BLAST
Repeati551 – 57222PbH1 5Add
BLAST
Repeati574 – 60330PbH1 6Add
BLAST

Sequence similaritiesi

Contains 6 PbH1 repeats.Curated

Keywords - Domaini

Repeat

Family and domain databases

Gene3Di2.160.20.10. 4 hits.
InterProiIPR003343. Big_2.
IPR008964. Invasin/intimin_cell_adhesion.
IPR006626. PbH1.
IPR012334. Pectin_lyas_fold.
IPR011050. Pectin_lyase_fold/virulence.
[Graphical view]
PfamiPF02368. Big_2. 1 hit.
[Graphical view]
SMARTiSM00710. PbH1. 6 hits.
[Graphical view]
SUPFAMiSSF49373. SSF49373. 1 hit.
SSF51126. SSF51126. 3 hits.

Sequencei

Sequence statusi: Complete.

B1V8K7-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MGTATAQPAL RPQTSTVIGG LHGAAVLDNT GRTVIDVTDF GADPSGKADS
60 70 80 90 100
AAAVSAAMAH AKTVGGPTTL HFPTGTYHIW PERTPKRELY VSNTVGSDQA
110 120 130 140 150
FRTKNIGILV EDMRDVVVDG GGSRIVNHGF QTVFAAIRSS DVRFTNFSQT
160 170 180 190 200
WVAPKTVDIT VADAGVVSGQ AYRIIDIPET YDYAVEGTSV RWNGERGPAT
210 220 230 240 250
GQPYWTGTNS FDYSQVHDPA TNRTWRTSNP VFPERHEDHR PRRRQVRITY
260 270 280 290 300
GDSTAPGDRG YVYQMREVTR DTPGALFWES SRVTVDHLRL GYLHGFGIVG
310 320 330 340 350
QLSEDIGIDS VTFKADRGSG RVTSGFADHI QMSGVKGTVR ITNSVFDNPQ
360 370 380 390 400
DDPINIHGTY LQATAAERET LQLRYMHNET SGFPQFYPGD TIELVDKRTM
410 420 430 440 450
LAAPGATAKV VSVTGPTGSG VPAGTDPDTY LRTMTVVLDR TLPAAVLAAP
460 470 480 490 500
GDYVAENTTY TPTVEITGNT FQAVPTRGIL VTTRRPVRIE NNRFDGMSMA
510 520 530 540 550
SIYISSDARS WYESGPVRNV TIRGNVFDRP ASPVIFFDPT NQDFVAGQPV
560 570 580 590 600
HRNVLIEDND FNLTGGTILS GRGVGGLTFR DNRVERYPHL RLTGPSRALR
610 620 630 640 650
VGDTTTVTTD APPPSHTSPL FTFDGADDIT LANNTYGNGF NKRVNTANMD
660 670 680 690 700
VSEITVTADG LALNADSISS APVAVSYSSS RPKVATVDSE GVVKALSGGT
710 720
TSITARATIG GVRVTSNPVK VVVATER
Length:727
Mass (Da):78,244
Last modified:May 20, 2008 - v1
Checksum:i059E250ED79FB764
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti66 – 661G → I AA sequence (PubMed:17401360)Curated
Sequence conflicti76 – 761T → G AA sequence (PubMed:17401360)Curated
Sequence conflicti492 – 4921N → S AA sequence (PubMed:17401360)Curated
Sequence conflicti496 – 4961G → D AA sequence (PubMed:17401360)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AM259273 Genomic DNA. Translation: CAJ90659.1.

Cross-referencesi

Web resourcesi

Protein Spotlight

The juice of life - Issue 98 of October 2008

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AM259273 Genomic DNA. Translation: CAJ90659.1 .

3D structure databases

ProteinModelPortali B1V8K7.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

CAZyi GH110. Glycoside Hydrolase Family 110.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 2.160.20.10. 4 hits.
InterProi IPR003343. Big_2.
IPR008964. Invasin/intimin_cell_adhesion.
IPR006626. PbH1.
IPR012334. Pectin_lyas_fold.
IPR011050. Pectin_lyase_fold/virulence.
[Graphical view ]
Pfami PF02368. Big_2. 1 hit.
[Graphical view ]
SMARTi SM00710. PbH1. 6 hits.
[Graphical view ]
SUPFAMi SSF49373. SSF49373. 1 hit.
SSF51126. SSF51126. 3 hits.
ProtoNeti Search...

Publicationsi

  1. "Identification of a GH110 subfamily of alpha1,3-galactosidases: novel enzymes for removal of the alpha3Gal xenotransplantation antigen."
    Liu Q.P., Yuan H., Bennett E.P., Levery S.B., Nudelman E., Spence J., Pietz G., Saunders K., White T., Olsson M.L., Henrissat B., Sulzenbacher G., Clausen H.
    J. Biol. Chem. 283:8545-8554(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], ENZYME ACTIVITY.
  2. Cited for: PROTEIN SEQUENCE OF 34-77; 144-153; 273-280 AND 489-497, CATALYTIC ACTIVITY.

Entry informationi

Entry nameiGLAA_STRGU
AccessioniPrimary (citable) accession number: B1V8K7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 2, 2008
Last sequence update: May 20, 2008
Last modified: October 1, 2014
This is version 27 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. Protein Spotlight
    Protein Spotlight articles and cited UniProtKB/Swiss-Prot entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3