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B1Q3J6 (DNM1B_ORYSJ) Reviewed, UniProtKB/Swiss-Prot

Last modified March 19, 2014. Version 27. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
DNA (cytosine-5)-methyltransferase 1B

Short name=OsMET1b
EC=2.1.1.37
Alternative name(s):
DNA methyltransferase 1-2
Short name=OsMET1-2
Gene names
Name:MET1B
Synonyms:MET1-2
Ordered Locus Names:Os07g0182900, LOC_Os07g08500
ORF Names:OJ1506_G02.17
OrganismOryza sativa subsp. japonica (Rice) [Reference proteome]
Taxonomic identifier39947 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBEP cladeEhrhartoideaeOryzeaeOryza

Protein attributes

Sequence length1529 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Probably methylates CpG residues and maintains DNA methylation.

Catalytic activity

S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.

Subcellular location

Nucleus By similarity.

Tissue specificity

Expressed in roots, panicles, anthers, pistils, endosperm and imbibed embryos. Ref.1 Ref.4

Sequence similarities

Belongs to the class I-like SAM-binding methyltransferase superfamily. C5-methyltransferase family.

Contains 2 BAH domains.

Contains 1 SAM-dependent MTase C5-type domain.

Sequence caution

The sequence AK111461 differs from that shown. Reason: Frameshift at position 1320.

The sequence BAD30340.1 differs from that shown. Reason: Erroneous gene model prediction.

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: B1Q3J6-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: B1Q3J6-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-32: Missing.
     33-43: LDEGPLDATKE → MLSCGVFRKEK
Note: No experimental confirmation available.
Isoform 3 (identifier: B1Q3J6-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-43: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 15291529DNA (cytosine-5)-methyltransferase 1B
PRO_0000381940

Regions

Domain741 – 873133BAH 1
Domain910 – 1049140BAH 2
Domain1093 – 1527435SAM-dependent MTase C5-type

Sites

Active site11981 By similarity

Natural variations

Alternative sequence1 – 4343Missing in isoform 3.
VSP_037851
Alternative sequence1 – 3232Missing in isoform 2.
VSP_037852
Alternative sequence33 – 4311LDEGPLDATKE → MLSCGVFRKEK in isoform 2.
VSP_037853

Experimental info

Sequence conflict9231D → E in DAA01513. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified May 20, 2008. Version 1.
Checksum: F7471690926042C6

FASTA1,529171,908
        10         20         30         40         50         60 
MVKSPCSPVT TGKKRCRAKP QKKDEDTTDK GKLDEGPLDA TKEMNGVGKG DSRAACKRPR 

        70         80         90        100        110        120 
RAAACSDFKE KSVRLSDKSS VVATNGNKME EEEMDAVKLT KLGPEVQRPC RKLIDFILHD 

       130        140        150        160        170        180 
ADGKLQPFEM SEIDDFFITA LIMPMDDDLE KDRQKGVRCE GFGRIEDWAI SGYDEGTAVV 

       190        200        210        220        230        240 
WVSTEVADYE CVKPAGNYKS YYDHFYEKAQ VCVEVYRKLA RSVGGNPNLG LEELLASVVR 

       250        260        270        280        290        300 
SINAIKGYSG TLSKDFVISN GEFVYNQLIG LDETANTDDE KFATLPVLLA LRDGCKSRVE 

       310        320        330        340        350        360 
VSKLQPNISN GSLKINDAEC KEVSEDDDEK LARLLQQEEE WKMMKQRGKR GTTSQKNVYI 

       370        380        390        400        410        420 
KISEAEIAND YPLPAYYKPS SQEMDEYIFD SEDSFYSDVP VRILNNWALY NADSRLIPLE 

       430        440        450        460        470        480 
LIPMKAGAEN DIVVFGSGFM REDDGSCCST AESAKLSSSS SSNHQDAGVS IYLSPIKEWV 

       490        500        510        520        530        540 
IEFGGSMICI TIRTDVAWYK LRQPTKQYAP WCEPVLKTAR LSVSIITLLK EQSRASKLSF 

       550        560        570        580        590        600 
ADVIKKVAEF DKGSPAFVSS NVALVERYIV VHGQIILQQF SDFPDETIRR SAFATGLLMK 

       610        620        630        640        650        660 
MEQRRHTKLV MKKKVQVMRG ENLNPSATMG PASRRKVMRA TTTRLINRIW SDYYTHHFPE 

       670        680        690        700        710        720 
DSKDADVNEA KEIDDELEEN EDEDAEEEAQ IEEENVSKTP PSTRSRKLVS QTCKEIRWEG 

       730        740        750        760        770        780 
EAIGKTPSGE ALYKCAYVRE LRINLGRTVA LEDDSGELVM CFVEYMFQKL NGAKMVHGRL 

       790        800        810        820        830        840 
LQKGSETVLG NAANERDLFL TNECLEFELE DIKELMSVNL QSLPWGHKYR KENAEADRIE 

       850        860        870        880        890        900 
RAKAEDRKKK GLPMEYLCKS LYWPEKGAFF SLPHDKLGLG NGFCSSCQQK EPDCDELQIL 

       910        920        930        940        950        960 
SKNSFIYRNI TYNVNDYLYI RPDFFSQEED RATFKGGRNV GLKPYVVCHL LDVHEPAGSR 

       970        980        990       1000       1010       1020 
KIHPASTKIS VRRFYRPDDI SSAKAYVSDI REVYYSENIV KVPVDMIEGK CEVKKKIDIS 

      1030       1040       1050       1060       1070       1080 
NSDVPVMVEH EFFCEHFYDP ATGALKQLPP NVKLMSVQQK ATGALKKNKG KQICESDQVD 

      1090       1100       1110       1120       1130       1140 
SDKCTKVSKE NRLATLDIFA GCGGLSEGLQ QAGVSFTKWA IEYEEPAGEA FTKNHPEAAV 

      1150       1160       1170       1180       1190       1200 
FVDNCNVILK AIMDKCGDAD DCISTSEAAE QAAKFSQDNI MNLPVPGEVE FINGGPPCQG 

      1210       1220       1230       1240       1250       1260 
FSGMNRFNQS PWSKVQCEMI LAFLSFAEYF RPRFFLLENV RNFVSFNKGQ TFRLTVASLL 

      1270       1280       1290       1300       1310       1320 
EMGYQVRFGI LEAGTFGVAQ SRKRAFIWAA APGETLPDWP EPMHVFASPE LKINLPDGKY 

      1330       1340       1350       1360       1370       1380 
YAAAKSTAGG APFRAITVRD TIGDLPKVEN GASKLLLEYG GEPISWFQKK IRGNTIALND 

      1390       1400       1410       1420       1430       1440 
HISKEMNELN LIRCQRIPKR PGCDWHDLPD EKVKLSSGQL VDLIPWCLPN TAKRHNQWKG 

      1450       1460       1470       1480       1490       1500 
LYGRLDWEGN FPTSVTDPQP MGKVGMCFHP DQDRIITVRE CARSQGFPDN YQFAGNIQSK 

      1510       1520 
HRQIGNAVPP PLAFALGRKL KEAVDAKRQ 

« Hide

Isoform 2 [UniParc].

Checksum: AB600B781CBF1610
Show »

FASTA1,497168,488
Isoform 3 [UniParc].

Checksum: 1245DCB4B74CEB3B
Show »

FASTA1,486167,208

References

« Hide 'large scale' references
[1]"Alternative splicing of the rice OsMET1 genes encoding maintenance DNA methyltransferase."
Yamauchi T., Moritoh S., Johzuka-Hisatomi Y., Ono A., Terada R., Nakamura I., Iida S.
J. Plant Physiol. 165:1774-1782(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], TISSUE SPECIFICITY, ALTERNATIVE SPLICING.
Strain: cv. Nipponbare.
[2]"The map-based sequence of the rice genome."
International rice genome sequencing project (IRGSP)
Nature 436:793-800(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Nipponbare.
[3]"Collection, mapping, and annotation of over 28,000 cDNA clones from japonica rice."
The rice full-length cDNA consortium
Science 301:376-379(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1085-1529.
Strain: cv. Nipponbare.
[4]"Characterization of two rice DNA methyltransferase genes and RNAi-mediated reactivation of a silenced transgene in rice callus."
Teerawanichpan P., Chandrasekharan M.B., Jiang Y., Narangajavana J., Hall T.C.
Planta 218:337-349(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION, TISSUE SPECIFICITY.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB362511 Genomic DNA. Translation: BAG15929.1.
AB362511 Genomic DNA. Translation: BAG15930.1.
AP003835 Genomic DNA. Translation: BAD30340.1. Sequence problems.
AK111461 mRNA. No translation available.
BK001405 Genomic DNA. Translation: DAA01513.1.

3D structure databases

ProteinModelPortalB1Q3J6.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

REBASE7659. M.OsaDnmt1B.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Organism-specific databases

GrameneB1Q3J6.

Phylogenomic databases

eggNOGCOG0270.

Gene expression databases

ArrayExpressB1Q3J6.

Family and domain databases

InterProIPR001025. BAH_dom.
IPR018117. C5_DNA_meth_AS.
IPR001525. C5_MeTfrase.
IPR022702. Cytosine_MeTrfase1_RFD.
IPR017198. DNA_C5-MeTrfase_1_euk.
[Graphical view]
PANTHERPTHR10629. PTHR10629. 1 hit.
PfamPF01426. BAH. 2 hits.
PF00145. DNA_methylase. 1 hit.
PF12047. DNMT1-RFD. 2 hits.
[Graphical view]
PIRSFPIRSF037404. DNMT1. 1 hit.
PRINTSPR00105. C5METTRFRASE.
SMARTSM00439. BAH. 2 hits.
[Graphical view]
TIGRFAMsTIGR00675. dcm. 1 hit.
PROSITEPS51038. BAH. 2 hits.
PS00094. C5_MTASE_1. 1 hit.
PS00095. C5_MTASE_2. 1 hit.
PS51679. SAM_MT_C5. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDNM1B_ORYSJ
AccessionPrimary (citable) accession number: B1Q3J6
Secondary accession number(s): B1Q3J7, Q69W70, Q7PC77
Entry history
Integrated into UniProtKB/Swiss-Prot: September 1, 2009
Last sequence update: May 20, 2008
Last modified: March 19, 2014
This is version 27 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Oryza sativa (rice)

Index of Oryza sativa entries and their corresponding gene designations