B1Q3J6 (DNM1B_ORYSJ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
DNA (cytosine-5)-methyltransferase 1B
Short name=OsMET1b
EC=2.1.1.37

Alternative name(s):
DNA methyltransferase 1-2
Short name=OsMET1-2

Gene names

Name:	MET1B
Synonyms:	MET1-2
Ordered Locus Names:	Os07g0182900, LOC_Os07g08500
ORF Names:	OJ1506_G02.17

Organism

Oryza sativa subsp. japonica (Rice) [Reference proteome]

Taxonomic identifier

39947 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › Liliopsida › commelinids › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza › Oryza sativa

Protein attributes

Sequence length	1529 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Probably methylates CpG residues and maintains DNA methylation.
Catalytic activity	S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.
Subcellular location	Nucleus By similarity.
Tissue specificity	Expressed in roots, panicles, anthers, pistils, endosperm and imbibed embryos. Ref.1 Ref.4
Sequence similarities	Belongs to the C5-methyltransferase family. Contains 2 BAH domains.
Sequence caution	The sequence AK111461 differs from that shown. Reason: Frameshift at position 1320. The sequence BAD30340.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Cellular component	Nucleus
Coding sequence diversity	Alternative splicing
Domain	Repeat
Ligand	DNA-binding S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	DNA (cytosine-5-)-methyltransferase activity Inferred from electronic annotation. Source: EC DNA binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: B1Q3J6-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: B1Q3J6-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-32: Missing.
     33-43: LDEGPLDATKE → MLSCGVFRKEK

Note: No experimental confirmation available.

Isoform 3 (identifier: B1Q3J6-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-43: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1529	1529	DNA (cytosine-5)-methyltransferase 1B		PRO_0000381940
Regions
	Domain	741 – 873	133	BAH 1
	Domain	910 – 1049	140	BAH 2
Sites
	Active site	1198	1	By similarity
Natural variations
	Alternative sequence	1 – 43	43	Missing in isoform 3.		VSP_037851
	Alternative sequence	1 – 32	32	Missing in isoform 2.		VSP_037852
	Alternative sequence	33 – 43	11	LDEGPLDATKE → MLSCGVFRKEK in isoform 2.		VSP_037853
Experimental info
	Sequence conflict	923	1	D → E in DAA01513. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified May 20, 2008. Version 1. Checksum: F7471690926042C6	FASTA	1,529	171,908
	10 20 30 40 50 60 MVKSPCSPVT TGKKRCRAKP QKKDEDTTDK GKLDEGPLDA TKEMNGVGKG DSRAACKRPR 70 80 90 100 110 120 RAAACSDFKE KSVRLSDKSS VVATNGNKME EEEMDAVKLT KLGPEVQRPC RKLIDFILHD 130 140 150 160 170 180 ADGKLQPFEM SEIDDFFITA LIMPMDDDLE KDRQKGVRCE GFGRIEDWAI SGYDEGTAVV 190 200 210 220 230 240 WVSTEVADYE CVKPAGNYKS YYDHFYEKAQ VCVEVYRKLA RSVGGNPNLG LEELLASVVR 250 260 270 280 290 300 SINAIKGYSG TLSKDFVISN GEFVYNQLIG LDETANTDDE KFATLPVLLA LRDGCKSRVE 310 320 330 340 350 360 VSKLQPNISN GSLKINDAEC KEVSEDDDEK LARLLQQEEE WKMMKQRGKR GTTSQKNVYI 370 380 390 400 410 420 KISEAEIAND YPLPAYYKPS SQEMDEYIFD SEDSFYSDVP VRILNNWALY NADSRLIPLE 430 440 450 460 470 480 LIPMKAGAEN DIVVFGSGFM REDDGSCCST AESAKLSSSS SSNHQDAGVS IYLSPIKEWV 490 500 510 520 530 540 IEFGGSMICI TIRTDVAWYK LRQPTKQYAP WCEPVLKTAR LSVSIITLLK EQSRASKLSF 550 560 570 580 590 600 ADVIKKVAEF DKGSPAFVSS NVALVERYIV VHGQIILQQF SDFPDETIRR SAFATGLLMK 610 620 630 640 650 660 MEQRRHTKLV MKKKVQVMRG ENLNPSATMG PASRRKVMRA TTTRLINRIW SDYYTHHFPE 670 680 690 700 710 720 DSKDADVNEA KEIDDELEEN EDEDAEEEAQ IEEENVSKTP PSTRSRKLVS QTCKEIRWEG 730 740 750 760 770 780 EAIGKTPSGE ALYKCAYVRE LRINLGRTVA LEDDSGELVM CFVEYMFQKL NGAKMVHGRL 790 800 810 820 830 840 LQKGSETVLG NAANERDLFL TNECLEFELE DIKELMSVNL QSLPWGHKYR KENAEADRIE 850 860 870 880 890 900 RAKAEDRKKK GLPMEYLCKS LYWPEKGAFF SLPHDKLGLG NGFCSSCQQK EPDCDELQIL 910 920 930 940 950 960 SKNSFIYRNI TYNVNDYLYI RPDFFSQEED RATFKGGRNV GLKPYVVCHL LDVHEPAGSR 970 980 990 1000 1010 1020 KIHPASTKIS VRRFYRPDDI SSAKAYVSDI REVYYSENIV KVPVDMIEGK CEVKKKIDIS 1030 1040 1050 1060 1070 1080 NSDVPVMVEH EFFCEHFYDP ATGALKQLPP NVKLMSVQQK ATGALKKNKG KQICESDQVD 1090 1100 1110 1120 1130 1140 SDKCTKVSKE NRLATLDIFA GCGGLSEGLQ QAGVSFTKWA IEYEEPAGEA FTKNHPEAAV 1150 1160 1170 1180 1190 1200 FVDNCNVILK AIMDKCGDAD DCISTSEAAE QAAKFSQDNI MNLPVPGEVE FINGGPPCQG 1210 1220 1230 1240 1250 1260 FSGMNRFNQS PWSKVQCEMI LAFLSFAEYF RPRFFLLENV RNFVSFNKGQ TFRLTVASLL 1270 1280 1290 1300 1310 1320 EMGYQVRFGI LEAGTFGVAQ SRKRAFIWAA APGETLPDWP EPMHVFASPE LKINLPDGKY 1330 1340 1350 1360 1370 1380 YAAAKSTAGG APFRAITVRD TIGDLPKVEN GASKLLLEYG GEPISWFQKK IRGNTIALND 1390 1400 1410 1420 1430 1440 HISKEMNELN LIRCQRIPKR PGCDWHDLPD EKVKLSSGQL VDLIPWCLPN TAKRHNQWKG 1450 1460 1470 1480 1490 1500 LYGRLDWEGN FPTSVTDPQP MGKVGMCFHP DQDRIITVRE CARSQGFPDN YQFAGNIQSK 1510 1520 HRQIGNAVPP PLAFALGRKL KEAVDAKRQ
	Isoform 2 [UniParc]. Checksum: AB600B781CBF1610	FASTA	1,497	168,488
	10 20 30 40 50 60 MLSCGVFRKE KMNGVGKGDS RAACKRPRRA AACSDFKEKS VRLSDKSSVV ATNGNKMEEE 70 80 90 100 110 120 EMDAVKLTKL GPEVQRPCRK LIDFILHDAD GKLQPFEMSE IDDFFITALI MPMDDDLEKD 130 140 150 160 170 180 RQKGVRCEGF GRIEDWAISG YDEGTAVVWV STEVADYECV KPAGNYKSYY DHFYEKAQVC 190 200 210 220 230 240 VEVYRKLARS VGGNPNLGLE ELLASVVRSI NAIKGYSGTL SKDFVISNGE FVYNQLIGLD 250 260 270 280 290 300 ETANTDDEKF ATLPVLLALR DGCKSRVEVS KLQPNISNGS LKINDAECKE VSEDDDEKLA 310 320 330 340 350 360 RLLQQEEEWK MMKQRGKRGT TSQKNVYIKI SEAEIANDYP LPAYYKPSSQ EMDEYIFDSE 370 380 390 400 410 420 DSFYSDVPVR ILNNWALYNA DSRLIPLELI PMKAGAENDI VVFGSGFMRE DDGSCCSTAE 430 440 450 460 470 480 SAKLSSSSSS NHQDAGVSIY LSPIKEWVIE FGGSMICITI RTDVAWYKLR QPTKQYAPWC 490 500 510 520 530 540 EPVLKTARLS VSIITLLKEQ SRASKLSFAD VIKKVAEFDK GSPAFVSSNV ALVERYIVVH 550 560 570 580 590 600 GQIILQQFSD FPDETIRRSA FATGLLMKME QRRHTKLVMK KKVQVMRGEN LNPSATMGPA 610 620 630 640 650 660 SRRKVMRATT TRLINRIWSD YYTHHFPEDS KDADVNEAKE IDDELEENED EDAEEEAQIE 670 680 690 700 710 720 EENVSKTPPS TRSRKLVSQT CKEIRWEGEA IGKTPSGEAL YKCAYVRELR INLGRTVALE 730 740 750 760 770 780 DDSGELVMCF VEYMFQKLNG AKMVHGRLLQ KGSETVLGNA ANERDLFLTN ECLEFELEDI 790 800 810 820 830 840 KELMSVNLQS LPWGHKYRKE NAEADRIERA KAEDRKKKGL PMEYLCKSLY WPEKGAFFSL 850 860 870 880 890 900 PHDKLGLGNG FCSSCQQKEP DCDELQILSK NSFIYRNITY NVNDYLYIRP DFFSQEEDRA 910 920 930 940 950 960 TFKGGRNVGL KPYVVCHLLD VHEPAGSRKI HPASTKISVR RFYRPDDISS AKAYVSDIRE 970 980 990 1000 1010 1020 VYYSENIVKV PVDMIEGKCE VKKKIDISNS DVPVMVEHEF FCEHFYDPAT GALKQLPPNV 1030 1040 1050 1060 1070 1080 KLMSVQQKAT GALKKNKGKQ ICESDQVDSD KCTKVSKENR LATLDIFAGC GGLSEGLQQA 1090 1100 1110 1120 1130 1140 GVSFTKWAIE YEEPAGEAFT KNHPEAAVFV DNCNVILKAI MDKCGDADDC ISTSEAAEQA 1150 1160 1170 1180 1190 1200 AKFSQDNIMN LPVPGEVEFI NGGPPCQGFS GMNRFNQSPW SKVQCEMILA FLSFAEYFRP 1210 1220 1230 1240 1250 1260 RFFLLENVRN FVSFNKGQTF RLTVASLLEM GYQVRFGILE AGTFGVAQSR KRAFIWAAAP 1270 1280 1290 1300 1310 1320 GETLPDWPEP MHVFASPELK INLPDGKYYA AAKSTAGGAP FRAITVRDTI GDLPKVENGA 1330 1340 1350 1360 1370 1380 SKLLLEYGGE PISWFQKKIR GNTIALNDHI SKEMNELNLI RCQRIPKRPG CDWHDLPDEK 1390 1400 1410 1420 1430 1440 VKLSSGQLVD LIPWCLPNTA KRHNQWKGLY GRLDWEGNFP TSVTDPQPMG KVGMCFHPDQ 1450 1460 1470 1480 1490 DRIITVRECA RSQGFPDNYQ FAGNIQSKHR QIGNAVPPPL AFALGRKLKE AVDAKRQ
	Isoform 3 [UniParc]. Checksum: 1245DCB4B74CEB3B	FASTA	1,486	167,208
	10 20 30 40 50 60 MNGVGKGDSR AACKRPRRAA ACSDFKEKSV RLSDKSSVVA TNGNKMEEEE MDAVKLTKLG 70 80 90 100 110 120 PEVQRPCRKL IDFILHDADG KLQPFEMSEI DDFFITALIM PMDDDLEKDR QKGVRCEGFG 130 140 150 160 170 180 RIEDWAISGY DEGTAVVWVS TEVADYECVK PAGNYKSYYD HFYEKAQVCV EVYRKLARSV 190 200 210 220 230 240 GGNPNLGLEE LLASVVRSIN AIKGYSGTLS KDFVISNGEF VYNQLIGLDE TANTDDEKFA 250 260 270 280 290 300 TLPVLLALRD GCKSRVEVSK LQPNISNGSL KINDAECKEV SEDDDEKLAR LLQQEEEWKM 310 320 330 340 350 360 MKQRGKRGTT SQKNVYIKIS EAEIANDYPL PAYYKPSSQE MDEYIFDSED SFYSDVPVRI 370 380 390 400 410 420 LNNWALYNAD SRLIPLELIP MKAGAENDIV VFGSGFMRED DGSCCSTAES AKLSSSSSSN 430 440 450 460 470 480 HQDAGVSIYL SPIKEWVIEF GGSMICITIR TDVAWYKLRQ PTKQYAPWCE PVLKTARLSV 490 500 510 520 530 540 SIITLLKEQS RASKLSFADV IKKVAEFDKG SPAFVSSNVA LVERYIVVHG QIILQQFSDF 550 560 570 580 590 600 PDETIRRSAF ATGLLMKMEQ RRHTKLVMKK KVQVMRGENL NPSATMGPAS RRKVMRATTT 610 620 630 640 650 660 RLINRIWSDY YTHHFPEDSK DADVNEAKEI DDELEENEDE DAEEEAQIEE ENVSKTPPST 670 680 690 700 710 720 RSRKLVSQTC KEIRWEGEAI GKTPSGEALY KCAYVRELRI NLGRTVALED DSGELVMCFV 730 740 750 760 770 780 EYMFQKLNGA KMVHGRLLQK GSETVLGNAA NERDLFLTNE CLEFELEDIK ELMSVNLQSL 790 800 810 820 830 840 PWGHKYRKEN AEADRIERAK AEDRKKKGLP MEYLCKSLYW PEKGAFFSLP HDKLGLGNGF 850 860 870 880 890 900 CSSCQQKEPD CDELQILSKN SFIYRNITYN VNDYLYIRPD FFSQEEDRAT FKGGRNVGLK 910 920 930 940 950 960 PYVVCHLLDV HEPAGSRKIH PASTKISVRR FYRPDDISSA KAYVSDIREV YYSENIVKVP 970 980 990 1000 1010 1020 VDMIEGKCEV KKKIDISNSD VPVMVEHEFF CEHFYDPATG ALKQLPPNVK LMSVQQKATG 1030 1040 1050 1060 1070 1080 ALKKNKGKQI CESDQVDSDK CTKVSKENRL ATLDIFAGCG GLSEGLQQAG VSFTKWAIEY 1090 1100 1110 1120 1130 1140 EEPAGEAFTK NHPEAAVFVD NCNVILKAIM DKCGDADDCI STSEAAEQAA KFSQDNIMNL 1150 1160 1170 1180 1190 1200 PVPGEVEFIN GGPPCQGFSG MNRFNQSPWS KVQCEMILAF LSFAEYFRPR FFLLENVRNF 1210 1220 1230 1240 1250 1260 VSFNKGQTFR LTVASLLEMG YQVRFGILEA GTFGVAQSRK RAFIWAAAPG ETLPDWPEPM 1270 1280 1290 1300 1310 1320 HVFASPELKI NLPDGKYYAA AKSTAGGAPF RAITVRDTIG DLPKVENGAS KLLLEYGGEP 1330 1340 1350 1360 1370 1380 ISWFQKKIRG NTIALNDHIS KEMNELNLIR CQRIPKRPGC DWHDLPDEKV KLSSGQLVDL 1390 1400 1410 1420 1430 1440 IPWCLPNTAK RHNQWKGLYG RLDWEGNFPT SVTDPQPMGK VGMCFHPDQD RIITVRECAR 1450 1460 1470 1480 SQGFPDNYQF AGNIQSKHRQ IGNAVPPPLA FALGRKLKEA VDAKRQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Alternative splicing of the rice OsMET1 genes encoding maintenance DNA methyltransferase." Yamauchi T., Moritoh S., Johzuka-Hisatomi Y., Ono A., Terada R., Nakamura I., Iida S. J. Plant Physiol. 165:1774-1782(2008) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], TISSUE SPECIFICITY, ALTERNATIVE SPLICING. Strain: cv. Nipponbare.
[2]	"The map-based sequence of the rice genome." International rice genome sequencing project (IRGSP) Nature 436:793-800(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[3]	"Collection, mapping, and annotation of over 28,000 cDNA clones from japonica rice." The rice full-length cDNA consortium Science 301:376-379(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1085-1529. Strain: cv. Nipponbare.
[4]	"Characterization of two rice DNA methyltransferase genes and RNAi-mediated reactivation of a silenced transgene in rice callus." Teerawanichpan P., Chandrasekharan M.B., Jiang Y., Narangajavana J., Hall T.C. Planta 218:337-349(2004) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION, TISSUE SPECIFICITY.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB362511 Genomic DNA. Translation: BAG15929.1. AB362511 Genomic DNA. Translation: BAG15930.1. AP003835 Genomic DNA. Translation: BAD30340.1. Sequence problems. AK111461 mRNA. No translation available. BK001405 Genomic DNA. Translation: DAA01513.1.
3D structure databases
ProteinModelPortal	B1Q3J6.
ModBase	Search...
Protein family/group databases
REBASE	7659. M.OsaDnmt1B.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Organism-specific databases
Gramene	B1Q3J6.
Phylogenomic databases
eggNOG	COG0270.
Gene expression databases
ArrayExpress	B1Q3J6.
Family and domain databases
InterPro	IPR001025. BAH_dom. IPR018117. C5_DNA_meth_AS. IPR001525. C5_MeTfrase. IPR022702. Cytosine_MeTrfase1_RFD. IPR017198. DNA_C5-MeTrfase_1_euk. [Graphical view]
PANTHER	PTHR10629. PTHR10629. 1 hit.
Pfam	PF01426. BAH. 2 hits. PF00145. DNA_methylase. 1 hit. PF12047. DNMT1-RFD. 2 hits. [Graphical view]
PIRSF	PIRSF037404. DNMT1. 1 hit.
PRINTS	PR00105. C5METTRFRASE.
SMART	SM00439. BAH. 2 hits. [Graphical view]
TIGRFAMs	TIGR00675. dcm. 1 hit.
PROSITE	PS51038. BAH. 2 hits. PS00094. C5_MTASE_1. 1 hit. PS00095. C5_MTASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DNM1B_ORYSJ

Accession

Primary (citable) accession number: B1Q3J6
Secondary accession number(s): B1Q3J7, Q69W70, Q7PC77

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 1, 2009
Last sequence update:	May 20, 2008
Last modified:	April 3, 2013
This is version 25 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program