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Protein

DNA (cytosine-5)-methyltransferase 1B

Gene

MET1B

Organism
Oryza sativa subsp. japonica (Rice)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Probably methylates CpG residues and maintains DNA methylation.

Catalytic activityi

S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei1198PROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDNA-binding, Methyltransferase, Transferase
LigandS-adenosyl-L-methionine

Enzyme and pathway databases

BRENDAi2.1.1.37 4460
ReactomeiR-OSA-212300 PRC2 methylates histones and DNA

Protein family/group databases

REBASEi7659 M.OsaDnmt1B

Names & Taxonomyi

Protein namesi
Recommended name:
DNA (cytosine-5)-methyltransferase 1B (EC:2.1.1.37)
Short name:
OsMET1b
Alternative name(s):
DNA methyltransferase 1-2
Short name:
OsMET1-2
Gene namesi
Name:MET1B
Synonyms:MET1-2
Ordered Locus Names:Os07g0182900, LOC_Os07g08500
ORF Names:OJ1506_G02.17
OrganismiOryza sativa subsp. japonica (Rice)
Taxonomic identifieri39947 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBOP cladeOryzoideaeOryzeaeOryzinaeOryzaOryza sativa
Proteomesi
  • UP000059680 Componenti: Chromosome 7

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003819401 – 1529DNA (cytosine-5)-methyltransferase 1BAdd BLAST1529

Proteomic databases

PaxDbiB1Q3J6

Expressioni

Tissue specificityi

Expressed in roots, panicles, anthers, pistils, endosperm and imbibed embryos.2 Publications

Gene expression databases

ExpressionAtlasiB1Q3J6 differential
GenevisibleiB1Q3J6 OS

Interactioni

Protein-protein interaction databases

STRINGi39947.LOC_Os07g08500.1

Structurei

3D structure databases

ProteinModelPortaliB1Q3J6
SMRiB1Q3J6
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini741 – 873BAH 1PROSITE-ProRule annotationAdd BLAST133
Domaini910 – 1049BAH 2PROSITE-ProRule annotationAdd BLAST140
Domaini1093 – 1527SAM-dependent MTase C5-typePROSITE-ProRule annotationAdd BLAST435

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. C5-methyltransferase family.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiENOG410IF68 Eukaryota
COG0270 LUCA
InParanoidiB1Q3J6
KOiK00558

Family and domain databases

InterProiView protein in InterPro
IPR001025 BAH_dom
IPR018117 C5_DNA_meth_AS
IPR001525 C5_MeTfrase
IPR031303 C5_meth_CS
IPR022702 Cytosine_MeTrfase1_RFD
IPR017198 DNMT1-like
IPR029063 SAM-dependent_MTases
PfamiView protein in Pfam
PF01426 BAH, 2 hits
PF00145 DNA_methylase, 1 hit
PF12047 DNMT1-RFD, 2 hits
PIRSFiPIRSF037404 DNMT1, 1 hit
PRINTSiPR00105 C5METTRFRASE
SMARTiView protein in SMART
SM00439 BAH, 2 hits
SUPFAMiSSF53335 SSF53335, 3 hits
TIGRFAMsiTIGR00675 dcm, 1 hit
PROSITEiView protein in PROSITE
PS51038 BAH, 2 hits
PS00094 C5_MTASE_1, 1 hit
PS00095 C5_MTASE_2, 1 hit
PS51679 SAM_MT_C5, 1 hit

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: B1Q3J6-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MVKSPCSPVT TGKKRCRAKP QKKDEDTTDK GKLDEGPLDA TKEMNGVGKG
60 70 80 90 100
DSRAACKRPR RAAACSDFKE KSVRLSDKSS VVATNGNKME EEEMDAVKLT
110 120 130 140 150
KLGPEVQRPC RKLIDFILHD ADGKLQPFEM SEIDDFFITA LIMPMDDDLE
160 170 180 190 200
KDRQKGVRCE GFGRIEDWAI SGYDEGTAVV WVSTEVADYE CVKPAGNYKS
210 220 230 240 250
YYDHFYEKAQ VCVEVYRKLA RSVGGNPNLG LEELLASVVR SINAIKGYSG
260 270 280 290 300
TLSKDFVISN GEFVYNQLIG LDETANTDDE KFATLPVLLA LRDGCKSRVE
310 320 330 340 350
VSKLQPNISN GSLKINDAEC KEVSEDDDEK LARLLQQEEE WKMMKQRGKR
360 370 380 390 400
GTTSQKNVYI KISEAEIAND YPLPAYYKPS SQEMDEYIFD SEDSFYSDVP
410 420 430 440 450
VRILNNWALY NADSRLIPLE LIPMKAGAEN DIVVFGSGFM REDDGSCCST
460 470 480 490 500
AESAKLSSSS SSNHQDAGVS IYLSPIKEWV IEFGGSMICI TIRTDVAWYK
510 520 530 540 550
LRQPTKQYAP WCEPVLKTAR LSVSIITLLK EQSRASKLSF ADVIKKVAEF
560 570 580 590 600
DKGSPAFVSS NVALVERYIV VHGQIILQQF SDFPDETIRR SAFATGLLMK
610 620 630 640 650
MEQRRHTKLV MKKKVQVMRG ENLNPSATMG PASRRKVMRA TTTRLINRIW
660 670 680 690 700
SDYYTHHFPE DSKDADVNEA KEIDDELEEN EDEDAEEEAQ IEEENVSKTP
710 720 730 740 750
PSTRSRKLVS QTCKEIRWEG EAIGKTPSGE ALYKCAYVRE LRINLGRTVA
760 770 780 790 800
LEDDSGELVM CFVEYMFQKL NGAKMVHGRL LQKGSETVLG NAANERDLFL
810 820 830 840 850
TNECLEFELE DIKELMSVNL QSLPWGHKYR KENAEADRIE RAKAEDRKKK
860 870 880 890 900
GLPMEYLCKS LYWPEKGAFF SLPHDKLGLG NGFCSSCQQK EPDCDELQIL
910 920 930 940 950
SKNSFIYRNI TYNVNDYLYI RPDFFSQEED RATFKGGRNV GLKPYVVCHL
960 970 980 990 1000
LDVHEPAGSR KIHPASTKIS VRRFYRPDDI SSAKAYVSDI REVYYSENIV
1010 1020 1030 1040 1050
KVPVDMIEGK CEVKKKIDIS NSDVPVMVEH EFFCEHFYDP ATGALKQLPP
1060 1070 1080 1090 1100
NVKLMSVQQK ATGALKKNKG KQICESDQVD SDKCTKVSKE NRLATLDIFA
1110 1120 1130 1140 1150
GCGGLSEGLQ QAGVSFTKWA IEYEEPAGEA FTKNHPEAAV FVDNCNVILK
1160 1170 1180 1190 1200
AIMDKCGDAD DCISTSEAAE QAAKFSQDNI MNLPVPGEVE FINGGPPCQG
1210 1220 1230 1240 1250
FSGMNRFNQS PWSKVQCEMI LAFLSFAEYF RPRFFLLENV RNFVSFNKGQ
1260 1270 1280 1290 1300
TFRLTVASLL EMGYQVRFGI LEAGTFGVAQ SRKRAFIWAA APGETLPDWP
1310 1320 1330 1340 1350
EPMHVFASPE LKINLPDGKY YAAAKSTAGG APFRAITVRD TIGDLPKVEN
1360 1370 1380 1390 1400
GASKLLLEYG GEPISWFQKK IRGNTIALND HISKEMNELN LIRCQRIPKR
1410 1420 1430 1440 1450
PGCDWHDLPD EKVKLSSGQL VDLIPWCLPN TAKRHNQWKG LYGRLDWEGN
1460 1470 1480 1490 1500
FPTSVTDPQP MGKVGMCFHP DQDRIITVRE CARSQGFPDN YQFAGNIQSK
1510 1520
HRQIGNAVPP PLAFALGRKL KEAVDAKRQ
Length:1,529
Mass (Da):171,908
Last modified:May 20, 2008 - v1
Checksum:iF7471690926042C6
GO
Isoform 2 (identifier: B1Q3J6-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-32: Missing.
     33-43: LDEGPLDATKE → MLSCGVFRKEK

Note: No experimental confirmation available.
Show »
Length:1,497
Mass (Da):168,488
Checksum:iAB600B781CBF1610
GO
Isoform 3 (identifier: B1Q3J6-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-43: Missing.

Show »
Length:1,486
Mass (Da):167,208
Checksum:i1245DCB4B74CEB3B
GO

Sequence cautioni

The sequence AK111461 differs from that shown. Reason: Frameshift at position 1320.Curated
The sequence BAD30340 differs from that shown. Reason: Erroneous gene model prediction.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti923D → E in DAA01513 (PubMed:18281124).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0378511 – 43Missing in isoform 3. CuratedAdd BLAST43
Alternative sequenceiVSP_0378521 – 32Missing in isoform 2. CuratedAdd BLAST32
Alternative sequenceiVSP_03785333 – 43LDEGPLDATKE → MLSCGVFRKEK in isoform 2. CuratedAdd BLAST11

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB362511 Genomic DNA Translation: BAG15929.1
AB362511 Genomic DNA Translation: BAG15930.1
AP003835 Genomic DNA Translation: BAD30340.1 Sequence problems.
AP014963 Genomic DNA Translation: BAT00335.1
AP014963 Genomic DNA Translation: BAT00336.1
AP014963 Genomic DNA Translation: BAT00337.1
AK111461 mRNA No translation available.
BK001405 Genomic DNA Translation: DAA01513.1
RefSeqiXP_015645008.1, XM_015789522.1 [B1Q3J6-1]
XP_015645009.1, XM_015789523.1 [B1Q3J6-1]
UniGeneiOs.57401

Genome annotation databases

EnsemblPlantsiOs07t0182900-01; Os07t0182900-01; Os07g0182900 [B1Q3J6-2]
Os07t0182900-02; Os07t0182900-02; Os07g0182900 [B1Q3J6-1]
Os07t0182900-03; Os07t0182900-03; Os07g0182900 [B1Q3J6-1]
Os07t0182900-04; Os07t0182900-04; Os07g0182900 [B1Q3J6-1]
Os07t0182900-05; Os07t0182900-05; Os07g0182900 [B1Q3J6-3]
Os07t0182900-06; Os07t0182900-06; Os07g0182900 [B1Q3J6-3]
GeneIDi4342575
GrameneiOs07t0182900-01; Os07t0182900-01; Os07g0182900 [B1Q3J6-2]
Os07t0182900-02; Os07t0182900-02; Os07g0182900 [B1Q3J6-1]
Os07t0182900-03; Os07t0182900-03; Os07g0182900 [B1Q3J6-1]
Os07t0182900-04; Os07t0182900-04; Os07g0182900 [B1Q3J6-1]
Os07t0182900-05; Os07t0182900-05; Os07g0182900 [B1Q3J6-3]
Os07t0182900-06; Os07t0182900-06; Os07g0182900 [B1Q3J6-3]
KEGGiosa:4342575

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiDNM1B_ORYSJ
AccessioniPrimary (citable) accession number: B1Q3J6
Secondary accession number(s): A0A0P0X2V1
, B1Q3J7, Q69W70, Q7PC77
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 1, 2009
Last sequence update: May 20, 2008
Last modified: May 23, 2018
This is version 54 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Oryza sativa (rice)
    Index of Oryza sativa entries and their corresponding gene designations
  2. SIMILARITY comments
    Index of protein domains and families
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