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B1NKS7

- VP3_ROTHL

UniProt

B1NKS7 - VP3_ROTHL

Protein

Protein VP3

Gene
N/A
Organism
Rotavirus A (strain Human/Philippines/L26/1987 G12-P1B[4]-I2-R2-C2-M1/M2-A2-N1-T2-E2-H1) (RV-A)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 21 (01 Oct 2014)
      Sequence version 1 (29 Apr 2008)
      Previous versions | rss
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    Functioni

    Multifunctional enzyme involved in mRNA capping. Catalyzes the formation of the 5' cap structure on the viral plus-strand transcripts. Specifically binds to GTP and displays guanylyltransferase and methyltransferase activities. Has affinity for ssRNA but not for dsRNA. Capping activity is non-specific and caps RNAs that initiate with either a G or an A residue. Together with VP1 polymerase, forms an enzyme complex positioned near the channels situated at each of the five-fold vertices of the core. Following infection, the outermost layer of the virus is lost, leaving a double-layered particle (DLP) made up of the core and VP6 shell. VP1 then catalyzes the transcription of fully conservative plus-strand genomic RNAs that are capped by VP3 and extruded through the DLP's channels into the cytoplasm where they function as mRNAs for translation of viral proteins. DLPs probably have an RNA triphosphatase activity as well, whereas open cores don't By similarity.By similarity

    Catalytic activityi

    GTP + (5')pp-Pur-mRNA = diphosphate + G(5')ppp-Pur-mRNA.
    S-adenosyl-L-methionine + G(5')pppR-RNA = S-adenosyl-L-homocysteine + m7G(5')pppR-RNA.

    GO - Molecular functioni

    1. GTP binding Source: UniProtKB-KW
    2. mRNA (guanine-N7-)-methyltransferase activity Source: UniProtKB-EC
    3. mRNA guanylyltransferase activity Source: UniProtKB-EC
    4. RNA binding Source: UniProtKB-KW

    GO - Biological processi

    1. viral process Source: InterPro

    Keywords - Molecular functioni

    Methyltransferase, Nucleotidyltransferase, Transferase

    Keywords - Biological processi

    mRNA capping, mRNA processing

    Keywords - Ligandi

    GTP-binding, Nucleotide-binding, RNA-binding, S-adenosyl-L-methionine

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Protein VP3
    Including the following 2 domains:
    mRNA guanylyltransferase (EC:2.7.7.50)
    mRNA (guanine-N(7)-)-methyltransferase (EC:2.1.1.56)
    OrganismiRotavirus A (strain Human/Philippines/L26/1987 G12-P1B[4]-I2-R2-C2-M1/M2-A2-N1-T2-E2-H1) (RV-A)
    Taxonomic identifieri10953 [NCBI]
    Taxonomic lineageiVirusesdsRNA virusesReoviridaeSedoreovirinaeRotavirusRotavirus A
    Virus hostiHomo sapiens (Human) [TaxID: 9606]
    ProteomesiUP000001459: Genome

    Subcellular locationi

    Virion Curated
    Note: Attached inside the inner capsid as a minor component. Also found in spherical cytoplasmic structures, called virus factories, that appear early after infection and are the site of viral replication and packaging Potential.Curated

    GO - Cellular componenti

    1. viral nucleocapsid Source: InterPro

    Keywords - Cellular componenti

    Virion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 835835Protein VP3PRO_0000368082Add
    BLAST

    Interactioni

    Subunit structurei

    Interacts with VP1 Potential. Interacts with VP2 By similarity.By similarityCurated

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the rotavirus VP3 family.Curated

    Family and domain databases

    InterProiIPR011181. VP3_Rotav.
    [Graphical view]
    PfamiPF06929. Rotavirus_VP3. 1 hit.
    [Graphical view]
    PIRSFiPIRSF004015. LigT_rotavirus. 1 hit.
    PROSITEiPS51589. SAM_MT56_VP3. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    B1NKS7-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKVLALRHSV AQVYADTQVY THDNSKDEYE NAFLISNLTT HNILYLNYSV    50
    KTLQILNKSG IAAIEIQKMD ELFTLIRCNF TYDYIDDVVY LHDYSYYTNN 100
    EIRTDQHWVT KTNIEDYLLP GWKLTYVGYN GSDTRGHYNF SFRCQNAATD 150
    DDAIIEYIYS DELDFQSFIL KKIKERMTTS LPIARLSNRV FRDKLFKTLS 200
    VNHDKVVNVG PRNESMFTFL DYPSIKQFSN GPYLVKDTIK LKQERWLGKR 250
    LSQFDIGQYK NMLNVLTTLY QYYDMYHEKP IVYMIGSAPS YWIYDVKQYS 300
    DLKFETWDPL DTPYSNLHHK ELFYINDVQK LKDNSILYID IRTDRGNMDW 350
    KEWRKVVERQ TADNLYIAYK YLSTGRAKIC CVKMTAMDLE LPISAKLLHH 400
    PTTEIRSEFY LMMDIWDSKN IKRFIPKGVL YSYINNIITE NVFIQQPFKL 450
    KTLKNEYVIA LYALSNDLNN REDVIKLINN QKKALITVRI NNTFKDEPKV 500
    GFKNIYDWTF LPTDFETSES IITSYDGCLG IFGLSISLAS KPTGNNHLFI 550
    LSGTDKYFKL DQFANHMSIS RRSHQIRFSE SATSYSGYIF RDLSNNNFNL 600
    IGTNVENSVS GHVYNALIYY RYNYSFDLKR WIYLHSTGKA SIEGGRYYEH 650
    APIELIYACR SAREFAKLQD DLTVLRYSNE IENYINKVYS ITYADDPNYF 700
    IGVKFKNIPY KYNVKVPHLT FGVLNISEQM LPDAIAILKK FKNELFGMDI 750
    TTSYTYMLSD EVYVANISGV LSTYFKIYNA FYKEQITFGQ SRMFIPHVTL 800
    SFSNEKTVRI DTTKLYIDSI YLRKIKGDTV FDMTG 835
    Length:835
    Mass (Da):98,004
    Last modified:April 29, 2008 - v1
    Checksum:iFB5B1097BAC863CF
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    EF583035 Genomic RNA. Translation: ABU87844.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    EF583035 Genomic RNA. Translation: ABU87844.1 .

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    InterProi IPR011181. VP3_Rotav.
    [Graphical view ]
    Pfami PF06929. Rotavirus_VP3. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF004015. LigT_rotavirus. 1 hit.
    PROSITEi PS51589. SAM_MT56_VP3. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Full genome-based classification of rotaviruses reveals a common origin between human Wa-Like and porcine rotavirus strains and human DS-1-like and bovine rotavirus strains."
      Matthijnssens J., Ciarlet M., Heiman E.M., Arijs I., Delbeke T., McDonald S.M., Palombo E.A., Iturriza-Gomara M., Maes P., Patton J.T., Rahman M., Van Ranst M.
      J. Virol. 82:3204-3219(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

    Entry informationi

    Entry nameiVP3_ROTHL
    AccessioniPrimary (citable) accession number: B1NKS7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 14, 2009
    Last sequence update: April 29, 2008
    Last modified: October 1, 2014
    This is version 21 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Multifunctional enzyme

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3