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Protein

Inner capsid protein VP2

Gene
N/A
Organism
Rotavirus A (strain RVA/Human/United States/DS-1/1976/G2P1B[4]) (RV-A) (Rotavirus A (strain DS1))
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Inner capsid protein that self-assembles to form an icosahedral capsid with a T=2 symmetry, which consists of 120 copies of VP2, with channels at each of its five-fold vertices. This capsid constitutes the innermost concentric layer of the viral mature particle. It encapsidates the polymerase VP1, the capping enzyme VP3 and the genomic dsRNA, thereby defining the core. The innermost VP2 capsid and the intermediate VP6 capsid remain intact following cell entry to protect the dsRNA from degradation and to prevent unfavorable antiviral responses in the host cell during all the replication cycle of the virus. Nascent transcripts are transcribed within the structural confines of this double-layered particle (DLP) and are extruded through the channels formed by VP2 N-termini. VP2 is required for the replicase activity of VP1 polymerase. Probably recruits a copy of a VP1-VP3 complex, potentially along with a segment of plus-strand RNA, as a decamer of VP2 assembles. May activate the autoinhibited VP1/RNA complex to coordinate packaging and genome replication.UniRule annotation

GO - Molecular functioni

Keywordsi

Molecular functionRNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Inner capsid protein VP2UniRule annotation
OrganismiRotavirus A (strain RVA/Human/United States/DS-1/1976/G2P1B[4]) (RV-A) (Rotavirus A (strain DS1))
Taxonomic identifieri10950 [NCBI]
Taxonomic lineageiVirusesdsRNA virusesReoviridaeSedoreovirinaeRotavirusRotavirus A
Virus hostiHomo sapiens (Human) [TaxID: 9606]
Proteomesi
  • UP000001457 Componenti: Genome

Subcellular locationi

  • Virion UniRule annotation
  • Note: Inner capsid protein. Also found in spherical cytoplasmic structures, called virus factories, that appear early after infection and are the site of viral replication and packaging.UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Capsid protein, Inner capsid protein, T=2 icosahedral capsid protein, Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003680631 – 879Inner capsid protein VP2Add BLAST879

Post-translational modificationi

Sumoylated with SUMO1 and SUMO2. Sumoylation of viral proteins seems to have a positive role on viral replication.UniRule annotation

Keywords - PTMi

Ubl conjugation

Proteomic databases

PRIDEiB1NKR8

Interactioni

Subunit structurei

Homodecamer; each decamer is made up of two conformers of VP2, called VP2A and VP2B. Interacts with a VP1-VP3 complex. Interacts with the intermediate capsid protein VP6. Interacts with NSP5. Interacts (via N-terminus) with NSP2.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei219Interaction with the intermediate capsid protein VP6UniRule annotation1
Sitei223Interaction with the intermediate capsid protein VP6UniRule annotation1
Sitei227Interaction with the intermediate capsid protein VP6UniRule annotation1
Sitei838Interaction with the intermediate capsid protein VP6UniRule annotation1
Sitei840Interaction with the intermediate capsid protein VP6UniRule annotation1

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 795-fold hub; involved in the encapsidation of VP1 and VP3UniRule annotationAdd BLAST79
Regioni393 – 413HydrophobicUniRule annotationAdd BLAST21
Regioni421 – 441HydrophobicUniRule annotationAdd BLAST21

Domaini

The N-terminus binds RNA. It is necessary for encapsidation of VP1 and VP3. The N-termini of 10 VP2 molecules form a cylindrical hub underneath each 5-fold axis of the inner capsid.UniRule annotation

Sequence similaritiesi

Belongs to the rotavirus VP2 family.UniRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

OrthoDBiVOG0900001T

Family and domain databases

HAMAPiMF_04123 Rota_VP2, 1 hit
MF_04127 Rota_VP2_A, 1 hit
InterProiView protein in InterPro
IPR007779 Rotavirus_VP2
PfamiView protein in Pfam
PF05087 Rota_VP2, 1 hit
ProDomiView protein in ProDom or Entries sharing at least one domain
PD008866 Rota_VP2, 1 hit

Sequencei

Sequence statusi: Complete.

B1NKR8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAYRKRGARR EANLNNNDRM QEKNDEKQDS NKIQLSDKVL SKKEEIVTDS
60 70 80 90 100
HEEVKITDEV KKSTKEESKQ LLEVLKTKEE HQKEIQYEIL QKTIPTFEPK
110 120 130 140 150
ETILKKLEDI KPELAKKQTK LFRIFEPKQL PIYRANGERE LRNRWCWKLK
160 170 180 190 200
KDTLPDGDYD VREYFLNLYD QVLTEMPDYL LLKDMAVENK NSRDAGKVVD
210 220 230 240 250
SETASICDAI FQDEETEGAV RRFIAEMRQR VQADRNVVNY PSILHPIDYA
260 270 280 290 300
FNEYFLQHQL VEPLNNDIIF NYIPERIRND VNYILNMDRN LPSTARYIRP
310 320 330 340 350
NLLQDRLNLH DNFESLWDTI TTSNYILARS IVPDLKELVS TEAQIQKMSQ
360 370 380 390 400
DLQLEALTIQ SETQFLTGIN SQAANDCFKT LIAAMLSQRT MSLDFVTTNY
410 420 430 440 450
MSLISGMWLL TVIPNDMFIR ESLVACQLAI VNTIIYPAFG MQRMHYRNGD
460 470 480 490 500
PQTPFQIAEQ QIQNFQVANW LHFVNNNQFR QAVIDGVLNQ VLNDNIRSGH
510 520 530 540 550
VINQLMEALM QLSRQQFPTM PVDYKRSIQR GILLLSNRLG QLVDLTRLLA
560 570 580 590 600
YNYETLMACI TMNMQHVQTL TTEKLQLTSV TSLCMLIGNA TVIPSPQTLF
610 620 630 640 650
HYYNVNVNFH SNYNERINDA VAIITAANRL NLYQKKMKAI VEDFLKRLYI
660 670 680 690 700
FDVSRVPDDQ MYRLRDRLRL LPVEIRRLDI FNLILMNMDQ IERASDKIAQ
710 720 730 740 750
GVIIAYRDMH LERDEMYGYV NIARNLDGFQ QINLEELMRS GDYAQITNML
760 770 780 790 800
LNNQPVALVG ALPFITDSSV ISLIAKLDAT VFAQIVKLRK VDTLKPILYK
810 820 830 840 850
INSDSNDFYL VANYDWVPTS TTKVYKQVPQ QFDFRNSMHM LTSNLTFTVY
860 870
SDLLAFVSAD TVEPINAVAF DNMRIMNEL
Length:879
Mass (Da):102,311
Last modified:April 29, 2008 - v1
Checksum:i71F14CF7655FD3D6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
EF583026 Genomic RNA Translation: ABU87835.1
DQ870506 Genomic RNA Translation: ABI58292.1

Similar proteinsi

Entry informationi

Entry nameiVP2_ROTHD
AccessioniPrimary (citable) accession number: B1NKR8
Secondary accession number(s): A7J3A7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: April 29, 2008
Last modified: May 23, 2018
This is version 27 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome
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Main funding by: National Institutes of Health