Reviewed, UniProtKB/Swiss-Prot B1NKR5 (VP3_ROTAD)

Names and origin

Protein names	Recommended name:     Protein VP3 Including the following 2 domains:     1- Recommended name:             mRNA guanylyltransferase               EC=2.7.7.50     2- Recommended name:             mRNA (guanine-N(7)-)-methyltransferase               EC=2.1.1.56
Organism	Rotavirus A (strain Human/United States/D/1974 G1-P1A[8]-I1-R1-C1-M1-A1-N1-T1-E1-H1) (RV-A) [Complete proteome]
Taxonomic identifier	578831 [NCBI]
Taxonomic lineage	Viruses › dsRNA viruses › Reoviridae › Rotavirus › Rotavirus A
Virus host	Homo sapiens (Human) [TaxID: 9606]

Protein attributes

Sequence length	835 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Multifunctional enzyme involved in mRNA capping. Catalyzes the formation of the 5' cap structure on the viral plus-strand transcripts. Specifically binds to GTP and displays guanylyltransferase and methyltransferase activities. Has affinity for ssRNA but not for dsRNA. Capping activity is non-specific and caps RNAs that initiate with either a G or an A residue. Together with VP1 polymerase, forms an enzyme complex positioned near the channels situated at each of the five-fold vertices of the core. Following infection, the outermost layer of the virus is lost, leaving a double-layered particle (DLP) made up of the core and VP6 shell. VP1 then catalyzes the transcription of fully conservative plus-strand genomic RNAs that are capped by VP3 and extruded through the DLP's channels into the cytoplasm where they function as mRNAs for translation of viral proteins. DLPs probably have an RNA triphosphatase activity as well, whereas open cores don't By similarity.
Catalytic activity	GTP + (5')pp-Pur-mRNA = diphosphate + G(5')ppp-Pur-mRNA. S-adenosyl-L-methionine + G(5')pppR-RNA = S-adenosyl-L-homocysteine + m7G(5')pppR-RNA.
Subunit structure	Interacts with VP1 Potential. Interacts with VP2 By similarity.
Subcellular location	Virion Potential. Note: Attached inside the inner capsid as a minor component. Also found in spherical cytoplasmic structures, called virus factories, that appear early after infection and are the site of viral replication and packaging Potential.
Sequence similarities	Belongs to the rotavirus VP3 family.

Ontologies

Keywords
Biological process	mRNA capping mRNA processing
Cellular component	Core protein Virion
Ligand	GTP-binding Nucleotide-binding RNA-binding S-adenosyl-L-methionine
Molecular function	Methyltransferase Nucleotidyltransferase Transferase
Technical term	Complete proteome Multifunctional enzyme
Gene Ontology (GO)
Biological process	mRNA capping Inferred from electronic annotation. Source: UniProtKB-KW viral reproduction Inferred from electronic annotation. Source: InterPro
Cellular component	viral nucleocapsid Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	GTP binding Inferred from electronic annotation. Source: UniProtKB-KW RNA binding Inferred from electronic annotation. Source: UniProtKB-KW mRNA (guanine-N7-)-methyltransferase activity Inferred from electronic annotation. Source: EC mRNA guanylyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 835	835	Protein VP3		PRO_0000368084
Regions
	Compositional bias	270 – 273	4	Poly-Tyr

Sequences

	Sequence		Length	Mass (Da)
	B1NKR5-1 [UniParc]. Last modified April 29, 2008. Version 1. Checksum: 616C214CD4C22EAB	FASTA	835	97,786
	10 20 30 40 50 60 MKVLALRHSV AQVYADTQTY LHDDSKDEYE NAFLISNLTT HNILYLNYSL KTLKILNKSG 70 80 90 100 110 120 IAAVEVQSPD ELFALIRCNF TYDYENNIVY LHDYSYYTNN EIRTDQHWIT KTDIIDYLLP 130 140 150 160 170 180 GWKLTYVGYN GKNTRGHYNF SFICQNAATD DDIIIEYIYS NELDFQNFLL RKIKERMTTS 190 200 210 220 230 240 LPIARLSNRV FRDKLFPSIV NIHKKVINVG PRNESMFTFL NFPTIKQFSN GAYIVKHTIK 250 260 270 280 290 300 LKQEKWLGKR VSQFDIGQYK NMLNVVTTIY YYYNLYYSKP IIYMLGSAPS YWIYDIKQYS 310 320 330 340 350 360 DFTFETWDPL DTPYSTTHHK ELFFDKDVNK LKDNSVLYID IRTDRGNMDW KEWRKIVEQQ 370 380 390 400 410 420 TVSNLNIAYK YLSTGKAKVC CVKLTAMDLE LPITAKLLHH PTTEVRSEFY AILDVWDIIT 430 440 450 460 470 480 IKRFIPKGVF YAFINNVTTE NVFIQPPFKL KTSPTDYIVA LYALSNDFNS RQDVINLINK 490 500 510 520 530 540 QKQSLITVRI NNTFKDEPKV NFKNIYDWTF LPTDFELKDS IITSYDGCLG IFGLSISLSS 550 560 570 580 590 600 KPTGNNHLFI INGTDKYDKL DQYANHMGIS RRSHQIRFSE SATSYSGYIF RDLSNNNFNL 610 620 630 640 650 660 IGTNVENSVS GHVYNALIYY RYNYAFDLKR WIYLHSIGKV AVEGGRYYEH APIELIYACR 670 680 690 700 710 720 SAKEFAILQD DLTVLRYANE IEGYINKVYS ITYADDPNYF IGIKFNSIPY EYDVKIPHLT 730 740 750 760 770 780 LGVLFISDNM IHDVITALKK MKTELFKMEI STSYTYMLSD NTYVANASGV LSTYFKLYNM 790 800 810 820 830 FYRNHITFGQ SRMFIPHITL SFSNKRTVRI ESIKLRINSI YLRKIKGETV FDMSE

References

[1]

"Full genome-based classification of rotaviruses reveals a common origin between human Wa-Like and porcine rotavirus strains and human DS-1-like and bovine rotavirus strains." Matthijnssens J., Ciarlet M., Heiman E.M., Arijs I., Delbeke T., McDonald S.M., Palombo E.A., Iturriza-Gomara M., Maes P., Patton J.T., Rahman M., Van Ranst M. J. Virol. 82:3204-3219(2008) [PubMed: 18216098] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	EF583023 Genomic RNA. Translation: ABU87832.1.
3D structure databases
ModBase	Search...
Family and domain databases
InterPro	IPR011181. Pesterase_Rotav. IPR009697. Rotavirus_VP3. [Graphical view]
Pfam	PF06929. Rotavirus_VP3. 1 hit. [Graphical view]
PIRSF	PIRSF004015. LigT_rotavirus. 1 hit.
ProtoNet	Search...

Entry information

Entry name

VP3_ROTAD

Accession

Primary (citable) accession number: B1NKR5

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	April 29, 2008
Last modified:	June 16, 2009
This is version 8 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Virus (Virus annotation project)