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B1NKR1

- VP3_ROTH7

UniProt

B1NKR1 - VP3_ROTH7

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Protein

Protein VP3

Gene
N/A
Organism
Rotavirus A (isolate Human/United Kingdom/A64/1987 G10-P11[14]-I2-R2-C2-M1-A3-N2-T6-E2-H3) (RV-A)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Multifunctional enzyme involved in mRNA capping. Catalyzes the formation of the 5' cap structure on the viral plus-strand transcripts. Specifically binds to GTP and displays guanylyltransferase and methyltransferase activities. Has affinity for ssRNA but not for dsRNA. Capping activity is non-specific and caps RNAs that initiate with either a G or an A residue. Together with VP1 polymerase, forms an enzyme complex positioned near the channels situated at each of the five-fold vertices of the core. Following infection, the outermost layer of the virus is lost, leaving a double-layered particle (DLP) made up of the core and VP6 shell. VP1 then catalyzes the transcription of fully conservative plus-strand genomic RNAs that are capped by VP3 and extruded through the DLP's channels into the cytoplasm where they function as mRNAs for translation of viral proteins. DLPs probably have an RNA triphosphatase activity as well, whereas open cores don't (By similarity).By similarity

Catalytic activityi

GTP + (5')pp-Pur-mRNA = diphosphate + G(5')ppp-Pur-mRNA.
S-adenosyl-L-methionine + G(5')pppR-RNA = S-adenosyl-L-homocysteine + m7G(5')pppR-RNA.

GO - Molecular functioni

  1. GTP binding Source: UniProtKB-KW
  2. mRNA (guanine-N7-)-methyltransferase activity Source: UniProtKB-EC
  3. mRNA guanylyltransferase activity Source: UniProtKB-EC
  4. RNA binding Source: UniProtKB-KW

GO - Biological processi

  1. viral process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Nucleotidyltransferase, Transferase

Keywords - Biological processi

mRNA capping, mRNA processing

Keywords - Ligandi

GTP-binding, Nucleotide-binding, RNA-binding, S-adenosyl-L-methionine

Names & Taxonomyi

Protein namesi
Recommended name:
Protein VP3
Including the following 2 domains:
mRNA guanylyltransferase (EC:2.7.7.50)
mRNA (guanine-N(7)-)-methyltransferase (EC:2.1.1.56)
OrganismiRotavirus A (isolate Human/United Kingdom/A64/1987 G10-P11[14]-I2-R2-C2-M1-A3-N2-T6-E2-H3) (RV-A)
Taxonomic identifieri578827 [NCBI]
Taxonomic lineageiVirusesdsRNA virusesReoviridaeSedoreovirinaeRotavirusRotavirus A
Virus hostiHomo sapiens (Human) [TaxID: 9606]
ProteomesiUP000001456: Genome

Subcellular locationi

Virion Curated
Note: Attached inside the inner capsid as a minor component. Also found in spherical cytoplasmic structures, called virus factories, that appear early after infection and are the site of viral replication and packaging (Potential).Curated

GO - Cellular componenti

  1. viral nucleocapsid Source: InterPro
Complete GO annotation...

Keywords - Cellular componenti

Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 835835Protein VP3PRO_0000368069Add
BLAST

Interactioni

Subunit structurei

Interacts with VP1 (Potential). Interacts with VP2 (By similarity).By similarityCurated

Family & Domainsi

Sequence similaritiesi

Belongs to the rotavirus VP3 family.Curated

Family and domain databases

InterProiIPR011181. VP3_Rotav.
[Graphical view]
PfamiPF06929. Rotavirus_VP3. 1 hit.
[Graphical view]
PIRSFiPIRSF004015. LigT_rotavirus. 1 hit.
PROSITEiPS51589. SAM_MT56_VP3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B1NKR1-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKVLALRHSV AQVYADTQIY THDETKDDYE NAFLISNLTT HNILYLNYSV
60 70 80 90 100
KTLQILNKSG IAAVEIQKMD ELFTLIRCNF TYDYIEDIVY LHDYSYYTNN
110 120 130 140 150
EIRTDQHWVT KTNIEDYLLP GWKLTYVGYN GSDTRGHYNF SFKCQNAATD
160 170 180 190 200
DDAIIEYIYS NELDFQNFIL KKIKERMTTS LPIARLSNRV FRDKLFKTLV
210 220 230 240 250
SDHSKVVNVG PRNESMFTFL DHPSIKQFSN GPYLVKDTIK LKQERWLGKR
260 270 280 290 300
LSQFDIGQYK NMLNVLTTLY QYYDMYHEKP IIYMIGSAPS YWIYDVKQYS
310 320 330 340 350
NLKFETWDPL DTPYSDLHHK ELFYISDVTK LKDNSILYVD IRTDRENMDW
360 370 380 390 400
KTWRKIVEEQ TINNLNIAYR YLSTGKAKVC CVKMTAMDLE LPISAKLLHH
410 420 430 440 450
PTTEIRSEFY LIMDIWDSKN IKRFIPKGVL YSYINNVITE NVFIQQPFKL
460 470 480 490 500
KTLRNEYVVA LYALSNDFNN REDVIKLVNN QKNALITVRI NNTFKDEPKV
510 520 530 540 550
GFKDIYDWTF LPTDFETNES IITSYDGCLG MFGLSISLAS KPTGNNHLFI
560 570 580 590 600
LSGTNKYFKL DQFANHMSIS RRSHQIRFSE SATSYSGYIF RDLSNNNFNL
610 620 630 640 650
IGTNVENSVS GHVYNALIYY RYNYSFDLKR WIYLHSTNKA SIEGGRYYEH
660 670 680 690 700
APIELIYACR SAREFARLQD DLTVLRYSNE IENYINKVYS ITYADDPNYF
710 720 730 740 750
IGIKFKNIPY EYDVKVPHLT FGVLNISNSM VPDVVAILKK FKSELFRMDV
760 770 780 790 800
TTSYTYMLSD EIYVANVSGV LSTYFKLYNA FYKEQITFGQ SRMFIPHITL
810 820 830
SFSDKKVVRI DSTRLNIDFI YLRKIKGDTV FDMAE
Length:835
Mass (Da):98,162
Last modified:April 29, 2008 - v1
Checksum:i80FA760D4C91A579
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
EF583019 Genomic RNA. Translation: ABU87828.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
EF583019 Genomic RNA. Translation: ABU87828.1 .

3D structure databases

ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

InterProi IPR011181. VP3_Rotav.
[Graphical view ]
Pfami PF06929. Rotavirus_VP3. 1 hit.
[Graphical view ]
PIRSFi PIRSF004015. LigT_rotavirus. 1 hit.
PROSITEi PS51589. SAM_MT56_VP3. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Full genome-based classification of rotaviruses reveals a common origin between human Wa-Like and porcine rotavirus strains and human DS-1-like and bovine rotavirus strains."
    Matthijnssens J., Ciarlet M., Heiman E.M., Arijs I., Delbeke T., McDonald S.M., Palombo E.A., Iturriza-Gomara M., Maes P., Patton J.T., Rahman M., Van Ranst M.
    J. Virol. 82:3204-3219(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

Entry informationi

Entry nameiVP3_ROTH7
AccessioniPrimary (citable) accession number: B1NKR1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: April 29, 2008
Last modified: October 1, 2014
This is version 21 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3