ID   B1N089_LEUCK            Unreviewed;       505 AA.
AC   B1N089;
DT   29-APR-2008, integrated into UniProtKB/TrEMBL.
DT   29-APR-2008, sequence version 1.
DT   27-MAR-2024, entry version 79.
DE   RecName: Full=Phosphoenolpyruvate carboxylase {ECO:0000256|HAMAP-Rule:MF_01904};
DE            Short=PEPC {ECO:0000256|HAMAP-Rule:MF_01904};
DE            Short=PEPCase {ECO:0000256|HAMAP-Rule:MF_01904};
DE            EC=4.1.1.31 {ECO:0000256|HAMAP-Rule:MF_01904};
GN   Name=ppcA {ECO:0000256|HAMAP-Rule:MF_01904};
GN   OrderedLocusNames=LCK_01367 {ECO:0000313|EMBL:ACA83191.1};
OS   Leuconostoc citreum (strain KM20).
OC   Bacteria; Bacillota; Bacilli; Lactobacillales; Lactobacillaceae;
OC   Leuconostoc.
OX   NCBI_TaxID=349519 {ECO:0000313|EMBL:ACA83191.1, ECO:0000313|Proteomes:UP000002166};
RN   [1] {ECO:0000313|EMBL:ACA83191.1, ECO:0000313|Proteomes:UP000002166}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=KM20 {ECO:0000313|EMBL:ACA83191.1,
RC   ECO:0000313|Proteomes:UP000002166};
RX   PubMed=18281406; DOI=10.1128/JB.01862-07;
RA   Kim J.F., Jeong H., Lee J.-S., Choi S.-H., Ha M., Hur C.-G., Kim J.-S.,
RA   Lee S., Park H.-S., Park Y.-H., Oh T.K.;
RT   "Complete genome sequence of Leuconostoc citreum KM20.";
RL   J. Bacteriol. 190:3093-3094(2008).
CC   -!- FUNCTION: Catalyzes the irreversible beta-carboxylation of
CC       phosphoenolpyruvate (PEP) to form oxaloacetate (OAA), a four-carbon
CC       dicarboxylic acid source for the tricarboxylic acid cycle.
CC       {ECO:0000256|HAMAP-Rule:MF_01904}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=oxaloacetate + phosphate = hydrogencarbonate +
CC         phosphoenolpyruvate; Xref=Rhea:RHEA:28370, ChEBI:CHEBI:16452,
CC         ChEBI:CHEBI:17544, ChEBI:CHEBI:43474, ChEBI:CHEBI:58702; EC=4.1.1.31;
CC         Evidence={ECO:0000256|HAMAP-Rule:MF_01904};
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000256|HAMAP-
CC         Rule:MF_01904};
CC   -!- SUBUNIT: Homotetramer. {ECO:0000256|HAMAP-Rule:MF_01904}.
CC   -!- SIMILARITY: Belongs to the PEPCase type 2 family. {ECO:0000256|HAMAP-
CC       Rule:MF_01904}.
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DR   EMBL; DQ489736; ACA83191.1; -; Genomic_DNA.
DR   RefSeq; WP_004904811.1; NC_010471.1.
DR   AlphaFoldDB; B1N089; -.
DR   STRING; 349519.LCK_01367; -.
DR   GeneID; 61101615; -.
DR   KEGG; lci:LCK_01367; -.
DR   eggNOG; COG1892; Bacteria.
DR   HOGENOM; CLU_517433_0_0_9; -.
DR   OrthoDB; 5487470at2; -.
DR   Proteomes; UP000002166; Chromosome.
DR   GO; GO:0000287; F:magnesium ion binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0008964; F:phosphoenolpyruvate carboxylase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0015977; P:carbon fixation; IEA:UniProtKB-UniRule.
DR   GO; GO:0006107; P:oxaloacetate metabolic process; IEA:UniProtKB-UniRule.
DR   GO; GO:0006099; P:tricarboxylic acid cycle; IEA:InterPro.
DR   HAMAP; MF_01904; PEPcase_type2; 1.
DR   InterPro; IPR007566; PEP_COase_arc-type.
DR   InterPro; IPR015813; Pyrv/PenolPyrv_Kinase-like_dom.
DR   NCBIfam; TIGR02751; PEPCase_arch; 1.
DR   Pfam; PF14010; PEPcase_2; 1.
DR   PIRSF; PIRSF006677; UCP006677; 1.
DR   SUPFAM; SSF51621; Phosphoenolpyruvate/pyruvate domain; 1.
PE   3: Inferred from homology;
KW   Carbon dioxide fixation {ECO:0000256|HAMAP-Rule:MF_01904};
KW   Lyase {ECO:0000256|HAMAP-Rule:MF_01904};
KW   Magnesium {ECO:0000256|ARBA:ARBA00022842, ECO:0000256|HAMAP-Rule:MF_01904}.
SQ   SEQUENCE   505 AA;  57727 MW;  C44A2E8109FED3FE CRC64;
     MTERKIPTIM GTQHPDNANA PFWDSSQQPF ISAYREMNEA FENFSELNVD EYMWDWEGKH
     ADAAVIDRLF STEYDYFKTN QIGRDKFLTF RFPNIWEEKG YNLMQAMTAI LSSEDFAHDL
     GFAQRPLFET ILPMAQRADQ LIEMQVLFEK LANFKSVEFT RSDKNTPYIE MIPLFEDFDT
     QLHAPEILKE YLQLHEKHFG FRPKYLRVFL AGSDSALTAG FMSSITGNKL AIARLHEFAR
     EENIEIYPIS GTGSAIFRGG LSPKRIDRYI KEFPGVRTAT VQSAFRYDFP IEDVQSAISE
     LKAKLPVVEP LQISAEDQTI LAEVAEESAA FYAHTLDQLV PDMQPIFKAF PKRRDRRQHV
     GVLGYSRSVD GIELPRAINF TGAFYSIGVP PEFIGFGRAL ENLNADHLSA LVRNYPSMKK
     DFQELSHYVN LDALEVLKTQ SPAWAEVERD IMILKAIFNL EVGPQTREEQ QHAEIALQVV
     QIKDGAPIAV TALINRMAQL RHFLG
//