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Protein

tRNA (guanine-N(7)-)-methyltransferase

Gene

trmB

Organism
Leuconostoc citreum (strain KM20)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the formation of N7-methylguanine at position 46 (m7G46) in tRNA.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + guanine(46) in tRNA = S-adenosyl-L-homocysteine + N7-methylguanine(46) in tRNA.UniRule annotation

Pathwayi: N(7)-methylguanine-tRNA biosynthesis

This protein is involved in the pathway N(7)-methylguanine-tRNA biosynthesis, which is part of tRNA modification.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway N(7)-methylguanine-tRNA biosynthesis and in tRNA modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei46S-adenosyl-L-methionineUniRule annotation1
Binding sitei71S-adenosyl-L-methionineUniRule annotation1
Binding sitei100S-adenosyl-L-methionineUniRule annotation1
Active sitei122By similarity1
Binding sitei122S-adenosyl-L-methionineUniRule annotation1
Binding sitei126SubstrateUniRule annotation1
Binding sitei158SubstrateUniRule annotation1

GO - Molecular functioni

Keywordsi

Molecular functionMethyltransferase, Transferase
Biological processtRNA processing
LigandS-adenosyl-L-methionine

Enzyme and pathway databases

UniPathwayiUPA00989

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (guanine-N(7)-)-methyltransferaseUniRule annotation (EC:2.1.1.33UniRule annotation)
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferaseUniRule annotation
tRNA(m7G46)-methyltransferaseUniRule annotation
Gene namesi
Name:trmBUniRule annotation
Ordered Locus Names:LCK_01072
OrganismiLeuconostoc citreum (strain KM20)
Taxonomic identifieri349519 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesLeuconostocaceaeLeuconostoc
Proteomesi
  • UP000002166 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10001363541 – 219tRNA (guanine-N(7)-)-methyltransferaseAdd BLAST219

Interactioni

Protein-protein interaction databases

STRINGi349519.LCK_01072

Structurei

3D structure databases

ProteinModelPortaliB1MZE7
SMRiB1MZE7
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni199 – 202Substrate bindingUniRule annotation4

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CZ1 Bacteria
COG0220 LUCA
HOGENOMiHOG000251689
KOiK03439
OMAiWITFPDP

Family and domain databases

HAMAPiMF_01057 tRNA_methyltr_TrmB, 1 hit
InterProiView protein in InterPro
IPR029063 SAM-dependent_MTases
IPR003358 tRNA_(Gua-N-7)_MeTrfase_Trmb
PANTHERiPTHR23417 PTHR23417, 1 hit
SUPFAMiSSF53335 SSF53335, 1 hit
TIGRFAMsiTIGR00091 TIGR00091, 1 hit
PROSITEiView protein in PROSITE
PS51625 SAM_MT_TRMB, 1 hit

Sequencei

Sequence statusi: Complete.

B1MZE7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MHLRAKPWAT DWLAAHSDIV ITQGRATEQI GQWQKLFAKA QPIHVEIGSG
60 70 80 90 100
KGQFILGMAL AHPEINYIGM EIQETAVAIA ARKSFDQVGE LPNLRYIYGN
110 120 130 140 150
GNGVETYFEK AEVEKIYLNF SDPWPKTRHE SRRLTYKTFL QSYEAVLPEH
160 170 180 190 200
GEVEFKTDNR HLFEYSIVSF MNYGMRWATT DFTLDLHADA EKVIGNVETE
210
YEQKFMAKGQ PIYKIKAHF
Length:219
Mass (Da):25,236
Last modified:April 29, 2008 - v1
Checksum:i41292505021702BB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ489736 Genomic DNA Translation: ACA82899.1
RefSeqiWP_004907667.1, NC_010471.1

Genome annotation databases

EnsemblBacteriaiACA82899; ACA82899; LCK_01072
GeneIDi35824779
KEGGilci:LCK_01072

Similar proteinsi

Entry informationi

Entry nameiTRMB_LEUCK
AccessioniPrimary (citable) accession number: B1MZE7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: April 29, 2008
Last modified: April 25, 2018
This is version 68 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health