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B1MZE7 (TRMB_LEUCK) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 48. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
tRNA (guanine-N(7)-)-methyltransferase

EC=2.1.1.33
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferase
tRNA(m7G46)-methyltransferase
Gene names
Name:trmB
Ordered Locus Names:LCK_01072
OrganismLeuconostoc citreum (strain KM20) [Complete proteome] [HAMAP]
Taxonomic identifier349519 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliLactobacillalesLeuconostocaceaeLeuconostoc

Protein attributes

Sequence length219 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA By similarity. HAMAP-Rule MF_01057

Catalytic activity

S-adenosyl-L-methionine + guanine46 in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine46 in tRNA. HAMAP-Rule MF_01057

Pathway

tRNA modification; N(7)-methylguanine-tRNA biosynthesis. HAMAP-Rule MF_01057

Sequence similarities

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.

Ontologies

Keywords
   Biological processtRNA processing
   LigandS-adenosyl-L-methionine
   Molecular functionMethyltransferase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Molecular_functiontRNA (guanine-N7-)-methyltransferase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 219219tRNA (guanine-N(7)-)-methyltransferase HAMAP-Rule MF_01057
PRO_1000136354

Regions

Region199 – 2024Substrate binding By similarity

Sites

Active site1221 By similarity
Binding site461S-adenosyl-L-methionine By similarity
Binding site711S-adenosyl-L-methionine By similarity
Binding site1001S-adenosyl-L-methionine By similarity
Binding site1221S-adenosyl-L-methionine By similarity
Binding site1261Substrate By similarity
Binding site1581Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
B1MZE7 [UniParc].

Last modified April 29, 2008. Version 1.
Checksum: 41292505021702BB

FASTA21925,236
        10         20         30         40         50         60 
MHLRAKPWAT DWLAAHSDIV ITQGRATEQI GQWQKLFAKA QPIHVEIGSG KGQFILGMAL 

        70         80         90        100        110        120 
AHPEINYIGM EIQETAVAIA ARKSFDQVGE LPNLRYIYGN GNGVETYFEK AEVEKIYLNF 

       130        140        150        160        170        180 
SDPWPKTRHE SRRLTYKTFL QSYEAVLPEH GEVEFKTDNR HLFEYSIVSF MNYGMRWATT 

       190        200        210 
DFTLDLHADA EKVIGNVETE YEQKFMAKGQ PIYKIKAHF 

« Hide

References

[1]"Complete genome sequence of Leuconostoc citreum KM20."
Kim J.F., Jeong H., Lee J.-S., Choi S.-H., Ha M., Hur C.-G., Kim J.-S., Lee S., Park H.-S., Park Y.-H., Oh T.K.
J. Bacteriol. 190:3093-3094(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: KM20.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
DQ489736 Genomic DNA. Translation: ACA82899.1.
RefSeqYP_001728343.1. NC_010471.1.

3D structure databases

ProteinModelPortalB1MZE7.
SMRB1MZE7. Positions 9-219.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING349519.LCK_01072.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACA82899; ACA82899; LCK_01072.
GeneID6064036.
KEGGlci:LCK_01072.
PATRIC32615440. VBILeuCit37309_1197.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0220.
HOGENOMHOG000251689.
KOK03439.
OMADNVMTEY.
OrthoDBEOG6K6VBC.

Enzyme and pathway databases

BioCycLCIT349519:GHNF-1094-MONOMER.
UniPathwayUPA00989.

Family and domain databases

Gene3D3.40.50.150. 1 hit.
HAMAPMF_01057. tRNA_methyltr_TrmB.
InterProIPR029063. SAM-dependent_MTases-like.
IPR003358. tRNA_(Gua-N-7)_MeTrfase.
[Graphical view]
PfamPF02390. Methyltransf_4. 1 hit.
[Graphical view]
SUPFAMSSF53335. SSF53335. 1 hit.
TIGRFAMsTIGR00091. TIGR00091. 1 hit.
PROSITEPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTRMB_LEUCK
AccessionPrimary (citable) accession number: B1MZE7
Entry history
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: April 29, 2008
Last modified: July 9, 2014
This is version 48 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways