B1MYG2 (B1MYG2_LEUCK) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Submitted name:
DNA topoisomerase I EMBL ACA82564.1
EC=5.99.1.2 EMBL ACA82564.1

Gene names

Name:	topA EMBL ACA82564.1
Ordered Locus Names:	LCK_00732

Organism

Leuconostoc citreum (strain KM20) [Complete proteome] [HAMAP]

Taxonomic identifier

349519 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Lactobacillales › Leuconostoc

Protein attributes

Sequence length	697 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand than undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone By similarity. SAAS SAAS000380
Catalytic activity	ATP-independent breakage of single-stranded DNA, followed by passage and rejoining. SAAS SAAS005733
Sequence similarities	Belongs to the prokaryotic type I/III topoisomerase family. RuleBase RU004206 Contains 1 Toprim domain. SAAS SAAS005733

Ontologies

Keywords
Ligand	ATP-binding SAAS SAAS005733 DNA-binding SAAS SAAS005733 Nucleotide-binding
Molecular function	Isomerase Topoisomerase SAAS SAAS005733
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA topological change Inferred from electronic annotation. Source: InterPro
Cellular component	chromosome Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA topoisomerase type I activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequences

	Sequence		Length	Mass (Da)
	B1MYG2 [UniParc]. Last modified April 29, 2008. Version 1. Checksum: 86FB2B38E1CE6C94	FASTA	697	79,174
	10 20 30 40 50 60 MAESEKKKTT TTKKTKKTTT KTRKKNLVIV ESPSKAKTIE KYLGSTYKVI ASLGHVRDLP 70 80 90 100 110 120 KSTLGVDVDN NYEPKYINIR GKGDIIKGLR QAAKAAKNVY LASDPDREGE AIAWHLQHIL 130 140 150 160 170 180 QLDDTAPNRV VFNEITKDTV KNAFKTPRLI NQDLVDAQQA RRILDRLVGY SISPILWKTV 190 200 210 220 230 240 KRGLSAGRVQ SVALGLIIKR EKEIQSFEPE EYWTLDAVFK KGRSKIQSVF YGVDDKKQQL 250 260 270 280 290 300 PDNDSVQDVL RRLNRNEPFE IGKVEAKERK RNPQRPFTTS TLQQTANTQL NFRTRKTMMA 310 320 330 340 350 360 AQQLYEGINL GRGIGQTGLI TYMRTDSTRI SEMARHAATQ YIHETWGEEY AQHRLVKQPS 370 380 390 400 410 420 AEGIQDAHEA VRPTDVTRTP DSIKDKLTPD QFKLYSLIWS RFVASQMTPE ILDTMAVTIT 430 440 450 460 470 480 QNGVLFRANG SKTKFPGFTK AYPAAKEKDN TLPHLEEGDQ VKIEQTDPEQ HFTQPPARYS 490 500 510 520 530 540 EAALIKALEE NGVGRPSTYA PTLETIQKRN YVRLDARKFV PTELGEIVQT IVEESFPDVT 550 560 570 580 590 600 NTQFTARIEH ELDEVEVGAK YWVPVIDEFF KPFSKEVEKA ETTLEKIIMH DELAGIDCSV 610 620 630 640 650 660 CGAPMLVKMG RYGKFYACAR FPDCRHTETI VKEIGLLCPK CHKGQIVERK TKKQRTFYGC 670 680 690 SRYPDCDFVS WEKPTDKTLP DGTTPKENSK NQAKTTD

References

[1]	"Complete genome sequence of Leuconostoc citreum KM20." Kim J.F., Jeong H., Lee J.-S., Choi S.-H., Ha M., Hur C.-G., Kim J.-S., Lee S., Park H.-S., Park Y.-H., Oh T.K. J. Bacteriol. 190:3093-3094(2008) [PubMed: 18281406] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	DQ489736 Genomic DNA. Translation: ACA82564.1.
RefSeq	YP_001728008.1. NC_010471.1.
3D structure databases
ProteinModelPortal	B1MYG2.
ModBase	Search...
Protein-protein interaction databases
STRING	B1MYG2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6064239.
GenomeReviews	Gene locus LCK_00732 in contig DQ489736_GR.
KEGG	lci:LCK_00732.
PATRIC	32614751. VBILeuCit37309_0856.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG512935.
OMA	IERNIRH.
ProtClustDB	PRK05582.
Family and domain databases
InterPro	IPR000380. Topo_IA. IPR003601. Topo_IA_2. IPR013497. Topo_IA_cen. IPR013824. Topo_IA_cen_sub1. IPR013825. Topo_IA_cen_sub2. IPR023405. Topo_IA_core_domain. IPR003602. Topo_IA_DNA-bd. IPR013498. Topo_IA_Znf. IPR005733. TopoI_bac-type. IPR006171. Toprim_domain. [Graphical view]
Gene3D	G3DSA:1.10.460.10. Topo_IA_cen_sub1. 2 hits. G3DSA:2.70.20.10. Topo_IA_cen_sub2. 2 hits.
KO	K03168.
PANTHER	PTHR11390. Topo_IA. 1 hit.
Pfam	PF01131. Topoisom_bac. 1 hit. PF01751. Toprim. 1 hit. PF01396. zf-C4_Topoisom. 2 hits. [Graphical view]
PRINTS	PR00417. PRTPISMRASEI.
SMART	SM00437. TOP1Ac. 1 hit. SM00436. TOP1Bc. 1 hit. SM00493. TOPRIM. 1 hit. [Graphical view]
SUPFAM	SSF56712. Topo_IA_core. 1 hit.
TIGRFAMs	TIGR01051. TopA_bact. 1 hit.
ProtoNet	Search...

Entry information

Entry name

B1MYG2_LEUCK

Accession

Primary (citable) accession number: B1MYG2

Entry history

Integrated into UniProtKB/TrEMBL:	April 29, 2008
Last sequence update:	April 29, 2008
Last modified:	December 14, 2011
This is version 36 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)