Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

B1MY11 (SYL_LEUCK) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 47. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:LCK_00580
OrganismLeuconostoc citreum (strain KM20) [Complete proteome] [HAMAP]
Taxonomic identifier349519 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliLactobacillalesLeuconostoc

Protein attributes

Sequence length803 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 803803Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_1000091334

Regions

Motif40 – 5112"HIGH" region HAMAP-Rule MF_00049
Motif575 – 5795"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site5781ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
B1MY11 [UniParc].

Last modified April 29, 2008. Version 1.
Checksum: 2E4AF1E47D8BE59C

FASTA80391,586
        10         20         30         40         50         60 
MPYEHQQIEQ KWQKFWDDNQ TFATSDTTDK PKYYAVDMFP YPSGQGLHVG HPEGYTATDI 

        70         80         90        100        110        120 
MSRFKRAKGF NVLHPMGWDA FGLPAEQYAM KTGHNPADFT SQNIDHFREQ IKSLGLSYDW 

       130        140        150        160        170        180 
NREINTTDPS YYKWTQWIFE KLYEKGLAYE SNMMVNWDPI DRVVLANEEV IDGKSERSGN 

       190        200        210        220        230        240 
KVERKALRQW VLKITAYADR LVDDLDDLDW PEAIKEQQRN WIGRSKGAAV FFNVDHADAK 

       250        260        270        280        290        300 
IEVYTTRPDT LFGATYMVLA PEHELVDSIV TAEQKQAVAD YRAAIAAKSD LERTDLNKDK 

       310        320        330        340        350        360 
TGVFTGAYGI NPINGEKLPI WIADYVLASY GTGAIMAVPA HDDRDFEFAK KFDLAIKPVI 

       370        380        390        400        410        420 
AGGENDDVAY TGDGVHINSD FLDGLDKATA IDQAIDWLEA HQAGHAQTNF RLRDWIFSRQ 

       430        440        450        460        470        480 
RYWGEPIPVI HWEDGTTSLV PENELPLLLP HATELRPSGS GESPLANLTD WLEVTRDDGV 

       490        500        510        520        530        540 
KGRRETNTMP QWAGSSWYFL RYIDPHNDHQ LADPEKLKYW MNVDLYVGGA EHAVLHLLYA 

       550        560        570        580        590        600 
RFWHKFLYDL GVVPTKEPFQ KLVNQGMILG ANHEKMSKSK GNVVNPDDIV REYGADTLRV 

       610        620        630        640        650        660 
YEMFMGPLTQ SKPWSEEGIT GSRRWLDRVW RLLIDDEGQL RDHVTTVNPG DLDKIYHQTV 

       670        680        690        700        710        720 
KKVTDDLENM RFNTAISQMM VFVNDAYKSD ALPVTYMNGF IQLLAPFAPH LAEELWVRLG 

       730        740        750        760        770        780 
NRESISYVSW PTYDDTKLVD DTVEIIFQVN GKVRGKATVA RDINQDDMIT AAKADDNVQN 

       790        800 
FITGKTVRKV IAIPGKFVNI VVS 

« Hide

References

[1]"Complete genome sequence of Leuconostoc citreum KM20."
Kim J.F., Jeong H., Lee J.-S., Choi S.-H., Ha M., Hur C.-G., Kim J.-S., Lee S., Park H.-S., Park Y.-H., Oh T.K.
J. Bacteriol. 190:3093-3094(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: KM20.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
DQ489736 Genomic DNA. Translation: ACA82413.1.
RefSeqYP_001727857.1. NC_010471.1.

3D structure databases

ProteinModelPortalB1MY11.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING349519.LCK_00580.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACA82413; ACA82413; LCK_00580.
GeneID6063670.
KEGGlci:LCK_00580.
PATRIC32614445. VBILeuCit37309_0704.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000200748.
KOK01869.
OMAGIEHACM.
OrthoDBEOG63Z74X.
ProtClustDBPRK00390.

Enzyme and pathway databases

BioCycLCIT349519:GHNF-604-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase_bac/mito.
IPR025709. Leu_tRNA-synth_edit.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_LEUCK
AccessionPrimary (citable) accession number: B1MY11
Entry history
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: April 29, 2008
Last modified: April 16, 2014
This is version 47 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries