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Protein

Hydroxyethylthiazole kinase

Gene

thiM

Organism
Leuconostoc citreum (strain KM20)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the phosphorylation of the hydroxyl group of 4-methyl-5-beta-hydroxyethylthiazole (THZ).UniRule annotation

Catalytic activityi

ATP + 4-methyl-5-(2-hydroxyethyl)thiazole = ADP + 4-methyl-5-(2-phosphonooxyethyl)thiazole.UniRule annotation

Cofactori

Mg2+UniRule annotation

Pathwayi: thiamine diphosphate biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes 4-methyl-5-(2-phosphoethyl)-thiazole from 5-(2-hydroxyethyl)-4-methylthiazole.UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Hydroxyethylthiazole kinase (thiM)
This subpathway is part of the pathway thiamine diphosphate biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 4-methyl-5-(2-phosphoethyl)-thiazole from 5-(2-hydroxyethyl)-4-methylthiazole, the pathway thiamine diphosphate biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei38Substrate; via amide nitrogenUniRule annotation1
Binding sitei113ATPUniRule annotation1
Binding sitei158ATPUniRule annotation1
Binding sitei185Substrate; via amide nitrogenUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionKinase, Transferase
Biological processThiamine biosynthesis
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00060; UER00139

Names & Taxonomyi

Protein namesi
Recommended name:
Hydroxyethylthiazole kinaseUniRule annotation (EC:2.7.1.50UniRule annotation)
Alternative name(s):
4-methyl-5-beta-hydroxyethylthiazole kinaseUniRule annotation
Short name:
TH kinaseUniRule annotation
Short name:
Thz kinaseUniRule annotation
Gene namesi
Name:thiMUniRule annotation
Ordered Locus Names:LCK_00280
OrganismiLeuconostoc citreum (strain KM20)
Taxonomic identifieri349519 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesLeuconostocaceaeLeuconostoc
Proteomesi
  • UP000002166 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003838801 – 259Hydroxyethylthiazole kinaseAdd BLAST259

Interactioni

Protein-protein interaction databases

STRINGi349519.LCK_00280

Structurei

3D structure databases

ProteinModelPortaliB1MX61
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the Thz kinase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C4U Bacteria
COG2145 LUCA
HOGENOMiHOG000114352
KOiK00878
OMAiKRPLVHN

Family and domain databases

CDDicd01170 THZ_kinase, 1 hit
Gene3Di3.40.1190.20, 1 hit
HAMAPiMF_00228 Thz_kinase, 1 hit
InterProiView protein in InterPro
IPR000417 Hyethyz_kinase
IPR029056 Ribokinase-like
PANTHERiPTHR44423 PTHR44423, 1 hit
PfamiView protein in Pfam
PF02110 HK, 1 hit
PIRSFiPIRSF000513 Thz_kinase, 1 hit
PRINTSiPR01099 HYETHTZKNASE
SUPFAMiSSF53613 SSF53613, 1 hit

Sequencei

Sequence statusi: Complete.

B1MX61-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKLSTLNETT PLVFNYANYV TPQFVANAVN VIGGSPIMAR EVAEFSDLVA
60 70 80 90 100
VSDAVVVNTG TWRRAELLET VKLIQIANQT QTVVVLDPVA VGIPSRSVPV
110 120 130 140 150
QELLTNSKVD IIRGNAAEIA WFAGIDFASH GIDAVGSGNI EQIAQLAANK
160 170 180 190 200
TGAIIAMSGP SDVISDGKTT RVLPVNVPLL ASNVGTGDAL SAIIGAFNGD
210 220 230 240 250
EISLDNTVRA MAMMKLAGLT ASHQVTTPGY FANQVLDELY LLSESKLETF

IAKEVKKYE
Length:259
Mass (Da):27,404
Last modified:April 29, 2008 - v1
Checksum:i9C6AA0BDA1DA1CB6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ489736 Genomic DNA Translation: ACA82113.1
RefSeqiWP_012305048.1, NC_010471.1

Genome annotation databases

EnsemblBacteriaiACA82113; ACA82113; LCK_00280
GeneIDi35826326
KEGGilci:LCK_00280

Similar proteinsi

Entry informationi

Entry nameiTHIM_LEUCK
AccessioniPrimary (citable) accession number: B1MX61
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: April 29, 2008
Last modified: April 25, 2018
This is version 63 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

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