Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Hydroxyethylthiazole kinase

Gene

thiM

Organism
Leuconostoc citreum (strain KM20)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the phosphorylation of the hydroxyl group of 4-methyl-5-beta-hydroxyethylthiazole (THZ).UniRule annotation

Catalytic activityi

ATP + 4-methyl-5-(2-hydroxyethyl)thiazole = ADP + 4-methyl-5-(2-phosphonooxyethyl)thiazole.UniRule annotation

Cofactori

Mg2+UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei38 – 381Substrate; via amide nitrogenUniRule annotation
Binding sitei113 – 1131ATPUniRule annotation
Binding sitei158 – 1581ATPUniRule annotation
Binding sitei185 – 1851Substrate; via amide nitrogenUniRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. hydroxyethylthiazole kinase activity Source: UniProtKB-HAMAP
  3. magnesium ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. thiamine biosynthetic process Source: UniProtKB-KW
  2. thiamine diphosphate biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Thiamine biosynthesis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciLCIT349519:GHNF-296-MONOMER.
UniPathwayiUPA00060; UER00139.

Names & Taxonomyi

Protein namesi
Recommended name:
Hydroxyethylthiazole kinaseUniRule annotation (EC:2.7.1.50UniRule annotation)
Alternative name(s):
4-methyl-5-beta-hydroxyethylthiazole kinaseUniRule annotation
Short name:
TH kinaseUniRule annotation
Short name:
Thz kinaseUniRule annotation
Gene namesi
Name:thiMUniRule annotation
Ordered Locus Names:LCK_00280
OrganismiLeuconostoc citreum (strain KM20)
Taxonomic identifieri349519 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesLeuconostocaceaeLeuconostoc
ProteomesiUP000002166: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 259259Hydroxyethylthiazole kinasePRO_0000383880Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi349519.LCK_00280.

Structurei

3D structure databases

ProteinModelPortaliB1MX61.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the Thz kinase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG2145.
HOGENOMiHOG000114352.
KOiK00878.
OMAiSPVMAHA.
OrthoDBiEOG628F8M.

Family and domain databases

Gene3Di3.40.1190.20. 1 hit.
HAMAPiMF_00228. Thz_kinase.
InterProiIPR000417. Hyethyz_kinase.
IPR029056. Ribokinase-like.
[Graphical view]
PfamiPF02110. HK. 1 hit.
[Graphical view]
PIRSFiPIRSF000513. Thz_kinase. 1 hit.
PRINTSiPR01099. HYETHTZKNASE.
SUPFAMiSSF53613. SSF53613. 1 hit.

Sequencei

Sequence statusi: Complete.

B1MX61-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKLSTLNETT PLVFNYANYV TPQFVANAVN VIGGSPIMAR EVAEFSDLVA
60 70 80 90 100
VSDAVVVNTG TWRRAELLET VKLIQIANQT QTVVVLDPVA VGIPSRSVPV
110 120 130 140 150
QELLTNSKVD IIRGNAAEIA WFAGIDFASH GIDAVGSGNI EQIAQLAANK
160 170 180 190 200
TGAIIAMSGP SDVISDGKTT RVLPVNVPLL ASNVGTGDAL SAIIGAFNGD
210 220 230 240 250
EISLDNTVRA MAMMKLAGLT ASHQVTTPGY FANQVLDELY LLSESKLETF

IAKEVKKYE
Length:259
Mass (Da):27,404
Last modified:April 29, 2008 - v1
Checksum:i9C6AA0BDA1DA1CB6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ489736 Genomic DNA. Translation: ACA82113.1.
RefSeqiWP_012305048.1. NC_010471.1.
YP_001727557.1. NC_010471.1.

Genome annotation databases

EnsemblBacteriaiACA82113; ACA82113; LCK_00280.
GeneIDi6063080.
KEGGilci:LCK_00280.
PATRICi32613819. VBILeuCit37309_0399.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ489736 Genomic DNA. Translation: ACA82113.1.
RefSeqiWP_012305048.1. NC_010471.1.
YP_001727557.1. NC_010471.1.

3D structure databases

ProteinModelPortaliB1MX61.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi349519.LCK_00280.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACA82113; ACA82113; LCK_00280.
GeneIDi6063080.
KEGGilci:LCK_00280.
PATRICi32613819. VBILeuCit37309_0399.

Phylogenomic databases

eggNOGiCOG2145.
HOGENOMiHOG000114352.
KOiK00878.
OMAiSPVMAHA.
OrthoDBiEOG628F8M.

Enzyme and pathway databases

UniPathwayiUPA00060; UER00139.
BioCyciLCIT349519:GHNF-296-MONOMER.

Family and domain databases

Gene3Di3.40.1190.20. 1 hit.
HAMAPiMF_00228. Thz_kinase.
InterProiIPR000417. Hyethyz_kinase.
IPR029056. Ribokinase-like.
[Graphical view]
PfamiPF02110. HK. 1 hit.
[Graphical view]
PIRSFiPIRSF000513. Thz_kinase. 1 hit.
PRINTSiPR01099. HYETHTZKNASE.
SUPFAMiSSF53613. SSF53613. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: KM20.

Entry informationi

Entry nameiTHIM_LEUCK
AccessioniPrimary (citable) accession number: B1MX61
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: April 29, 2008
Last modified: March 4, 2015
This is version 53 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.