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Protein

D-alanine--D-alanine ligase

Gene

ddl

Organism
Leuconostoc citreum (strain KM20)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Cell wall formation.UniRule annotation

Catalytic activityi

ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine.UniRule annotation

Cofactori

Mg2+By similarity, Mn2+By similarityNote: Binds 2 magnesium or manganese ions per subunit.By similarity

Pathwayi: peptidoglycan biosynthesis

This protein is involved in the pathway peptidoglycan biosynthesis, which is part of Cell wall biogenesis.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway peptidoglycan biosynthesis and in Cell wall biogenesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi303Magnesium or manganese 1UniRule annotation1
Metal bindingi316Magnesium or manganese 1UniRule annotation1
Metal bindingi316Magnesium or manganese 2UniRule annotation1
Metal bindingi318Magnesium or manganese 2UniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi170 – 225ATPUniRule annotationAdd BLAST56

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigase
Biological processCell shape, Cell wall biogenesis/degradation, Peptidoglycan synthesis
LigandATP-binding, Magnesium, Manganese, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00219

Names & Taxonomyi

Protein namesi
Recommended name:
D-alanine--D-alanine ligaseUniRule annotation (EC:6.3.2.4UniRule annotation)
Alternative name(s):
D-Ala-D-Ala ligaseUniRule annotation
D-alanylalanine synthetaseUniRule annotation
Gene namesi
Name:ddlUniRule annotation
Ordered Locus Names:LCK_00212
OrganismiLeuconostoc citreum (strain KM20)
Taxonomic identifieri349519 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesLeuconostocaceaeLeuconostoc
Proteomesi
  • UP000002166 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000911921 – 377D-alanine--D-alanine ligaseAdd BLAST377

Interactioni

Protein-protein interaction databases

STRINGi349519.LCK_00212

Structurei

3D structure databases

ProteinModelPortaliB1MWZ3
SMRiB1MWZ3
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini140 – 349ATP-graspUniRule annotationAdd BLAST210

Sequence similaritiesi

Belongs to the D-alanine--D-alanine ligase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CPF Bacteria
COG1181 LUCA
HOGENOMiHOG000011593
KOiK01921
OMAiYETKYTE

Family and domain databases

Gene3Di3.30.1490.20, 1 hit
HAMAPiMF_00047 Dala_Dala_lig, 1 hit
InterProiView protein in InterPro
IPR011761 ATP-grasp
IPR013815 ATP_grasp_subdomain_1
IPR000291 D-Ala_lig_Van_CS
IPR005905 D_ala_D_ala
IPR011095 Dala_Dala_lig_C
IPR011127 Dala_Dala_lig_N
IPR016185 PreATP-grasp_dom_sf
PfamiView protein in Pfam
PF07478 Dala_Dala_lig_C, 1 hit
PF01820 Dala_Dala_lig_N, 1 hit
PIRSFiPIRSF039102 Ddl/VanB, 1 hit
SUPFAMiSSF52440 SSF52440, 1 hit
TIGRFAMsiTIGR01205 D_ala_D_alaTIGR, 1 hit
PROSITEiView protein in PROSITE
PS50975 ATP_GRASP, 1 hit
PS00843 DALA_DALA_LIGASE_1, 1 hit
PS00844 DALA_DALA_LIGASE_2, 1 hit

Sequencei

Sequence statusi: Complete.

B1MWZ3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTKKYVALIF GGNSSEHDVS KRSAQNFYDA ILATGKYRIT VFAIAQNGYV
60 70 80 90 100
LDPERSKRIL ALEDERPIVA DYMTTVDQQD PLSRINALRA AGDFDIFFPV
110 120 130 140 150
VHGNLGEDGT LQGLFKLLNK PYVGAPLRGH AVSFDKVLTK ELLTVNNIRN
160 170 180 190 200
TKYIVVDEKT AKTLTWAQVV KDLGDVVFVK AANQGSSVGV SRAKTADEFE
210 220 230 240 250
AALTDSFQYD YKVLIEAAVK GPRELEVGVI GNEDPIVSEI GAHHVPNQGD
260 270 280 290 300
GDAWYDYNNK FVDNSSVQFE IPANLPDAVT AEVKDMALKA YKVLDLRGEA
310 320 330 340 350
RMDFLLDENN VPYLGEPNTL PGFTNMSLFK RLWDYSDIDN VELVDKLIDY
360 370
GFAEFEKNAQ LSYEFVSLGE EKIGKFN
Length:377
Mass (Da):41,977
Last modified:April 29, 2008 - v1
Checksum:iB8F1510431CC35A5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ489736 Genomic DNA Translation: ACA82045.1
RefSeqiWP_004909228.1, NC_010471.1

Genome annotation databases

EnsemblBacteriaiACA82045; ACA82045; LCK_00212
GeneIDi35826290
KEGGilci:LCK_00212

Similar proteinsi

Entry informationi

Entry nameiDDL_LEUCK
AccessioniPrimary (citable) accession number: B1MWZ3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: April 29, 2008
Last modified: April 25, 2018
This is version 65 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health