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B1MWZ3 (DDL_LEUCK) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 43. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
D-alanine--D-alanine ligase

EC=6.3.2.4
Alternative name(s):
D-Ala-D-Ala ligase
D-alanylalanine synthetase
Gene names
Name:ddl
Ordered Locus Names:LCK_00212
OrganismLeuconostoc citreum (strain KM20) [Complete proteome] [HAMAP]
Taxonomic identifier349519 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliLactobacillalesLeuconostocaceaeLeuconostoc

Protein attributes

Sequence length377 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Cell wall formation By similarity. HAMAP-Rule MF_00047

Catalytic activity

ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine. HAMAP-Rule MF_00047

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity.

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP-Rule MF_00047

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00047.

Sequence similarities

Belongs to the D-alanine--D-alanine ligase family.

Contains 1 ATP-grasp domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 377377D-alanine--D-alanine ligase HAMAP-Rule MF_00047
PRO_1000091192

Regions

Domain140 – 349210ATP-grasp
Nucleotide binding170 – 22556ATP By similarity

Sites

Metal binding3031Magnesium or manganese 1 By similarity
Metal binding3161Magnesium or manganese 1 By similarity
Metal binding3161Magnesium or manganese 2 By similarity
Metal binding3181Magnesium or manganese 2 By similarity

Sequences

Sequence LengthMass (Da)Tools
B1MWZ3 [UniParc].

Last modified April 29, 2008. Version 1.
Checksum: B8F1510431CC35A5

FASTA37741,977
        10         20         30         40         50         60 
MTKKYVALIF GGNSSEHDVS KRSAQNFYDA ILATGKYRIT VFAIAQNGYV LDPERSKRIL 

        70         80         90        100        110        120 
ALEDERPIVA DYMTTVDQQD PLSRINALRA AGDFDIFFPV VHGNLGEDGT LQGLFKLLNK 

       130        140        150        160        170        180 
PYVGAPLRGH AVSFDKVLTK ELLTVNNIRN TKYIVVDEKT AKTLTWAQVV KDLGDVVFVK 

       190        200        210        220        230        240 
AANQGSSVGV SRAKTADEFE AALTDSFQYD YKVLIEAAVK GPRELEVGVI GNEDPIVSEI 

       250        260        270        280        290        300 
GAHHVPNQGD GDAWYDYNNK FVDNSSVQFE IPANLPDAVT AEVKDMALKA YKVLDLRGEA 

       310        320        330        340        350        360 
RMDFLLDENN VPYLGEPNTL PGFTNMSLFK RLWDYSDIDN VELVDKLIDY GFAEFEKNAQ 

       370 
LSYEFVSLGE EKIGKFN 

« Hide

References

[1]"Complete genome sequence of Leuconostoc citreum KM20."
Kim J.F., Jeong H., Lee J.-S., Choi S.-H., Ha M., Hur C.-G., Kim J.-S., Lee S., Park H.-S., Park Y.-H., Oh T.K.
J. Bacteriol. 190:3093-3094(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: KM20.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
DQ489736 Genomic DNA. Translation: ACA82045.1.
RefSeqYP_001727489.1. NC_010471.1.

3D structure databases

ProteinModelPortalB1MWZ3.
SMRB1MWZ3. Positions 3-362.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING349519.LCK_00212.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACA82045; ACA82045; LCK_00212.
GeneID6063608.
KEGGlci:LCK_00212.
PATRIC32613659. VBILeuCit37309_0330.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1181.
HOGENOMHOG000011593.
KOK01921.
OMACANREEL.
OrthoDBEOG64BQ73.

Enzyme and pathway databases

BioCycLCIT349519:GHNF-217-MONOMER.
UniPathwayUPA00219.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.20. 1 hit.
HAMAPMF_00047. Dala_Dala_lig.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR000291. D-Ala_lig_Van_CS.
IPR005905. D_ala_D_ala.
IPR011095. Dala_Dala_lig_C.
IPR011127. Dala_Dala_lig_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PANTHERPTHR23132. PTHR23132. 1 hit.
PfamPF07478. Dala_Dala_lig_C. 1 hit.
PF01820. Dala_Dala_lig_N. 1 hit.
[Graphical view]
SUPFAMSSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00843. DALA_DALA_LIGASE_1. 1 hit.
PS00844. DALA_DALA_LIGASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDDL_LEUCK
AccessionPrimary (citable) accession number: B1MWZ3
Entry history
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: April 29, 2008
Last modified: July 9, 2014
This is version 43 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways