Phosphatidylinositol 4-kinase beta - Callicebus moloch (Dusky titi monkey)

Names and origin

Protein names

Recommended name:
    Phosphatidylinositol 4-kinase beta
      Short name=PtdIns 4-kinase beta
      Short name=PI4K-beta
      Short name=PI4Kbeta
    EC=2.7.1.67

Gene names

Name:	PI4KB
Synonyms:	PIK4CB

Organism

Callicebus moloch (Dusky titi monkey)

Taxonomic identifier

9523 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Platyrrhini › Pitheciidae › Callicebinae › Callicebus

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Protein attributes

Sequence length	816 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

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General annotation (Comments)

Function	Phosphorylates phosphatidylinositol (PI) in the first committed step in the production of the second messenger inositol-1,4,5,-trisphosphate (PIP). May regulate Golgi disintegration/reorganization during mitosis, possibly via its phosphorylation By similarity. Involved in Golgi-to-plasma membrane trafficking By similarity.
Catalytic activity	ATP + 1-phosphatidyl-1D-myo-inositol = ADP + 1-phosphatidyl-1D-myo-inositol 4-phosphate.
Enzyme regulation	Inhibited by wortmannin By similarity. Increased kinase acitvity upon interaction with FREQ/NCS1 By similarity.
Subunit structure	Interacts with FREQ/NCS1 in a calcium-independent manner By similarity. Interacts with CALN1/CABP8 and CALN2/CABP7; in a calcium-dependent manner By similarity. This binding competes with FREQ/NCS1 binding By similarity. Interacts with ARF1 and ARF3 By similarity.
Subcellular location	Endomembrane system By similarity. Mitochondrion outer membrane; Peripheral membrane protein By similarity. Rough endoplasmic reticulum membrane; Peripheral membrane protein By similarity. Golgi apparatus By similarity. Note: Found in the outer membrane of mitochondria and membranes of the rough endoplasmic reticulum. Recruited to the Golgi complex by the small GTPase ARF to stimulate the synthesis of phosphatidylinositol 4,5-biphosphate (PIP2) on the Golgi complex By similarity.
Sequence similarities	Belongs to the PI3/PI4-kinase family. Type III PI4K subfamily. Contains 1 PI3K/PI4K domain.

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Ontologies

Keywords
Cellular component	Endoplasmic reticulum Golgi apparatus Membrane Mitochondrion Mitochondrion outer membrane
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	phosphoinositide phosphorylation Inferred from electronic annotation. Source: InterPro phosphoinositide-mediated signaling Inferred from electronic annotation. Source: InterPro
Cellular component	Golgi apparatus Inferred from electronic annotation. Source: UniProtKB-SubCell endomembrane system Inferred from electronic annotation. Source: UniProtKB-SubCell endoplasmic reticulum Inferred from electronic annotation. Source: UniProtKB-KW extrinsic to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell mitochondrial outer membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	1-phosphatidylinositol 4-kinase activity Inferred from electronic annotation. Source: EC ATP binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 816

816

Phosphatidylinositol 4-kinase beta

PRO_0000365164

Regions

Domain

559 – 765

207

PI3K/PI4K

Compositional bias

117 – 120

4

Poly-Arg

Amino acid modifications

Modified residue

258

1

Phosphoserine By similarity

Modified residue

263

1

Phosphothreonine By similarity

Modified residue

266

1

Phosphoserine By similarity

Modified residue

277

1

Phosphoserine By similarity

Modified residue

294

1

Phosphoserine By similarity

Modified residue

428

1

Phosphoserine By similarity

Modified residue

438

1

Phosphothreonine By similarity

Modified residue

511

1

Phosphoserine By similarity

Modified residue

517

1

Phosphothreonine By similarity

Modified residue

519

1

Phosphothreonine By similarity

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Sequences

Sequence

Length

Mass (Da)

Tools

B1MTG7-1 [UniParc].

Last modified April 29, 2008. Version 1.
Checksum: 0EF3788F9300D08D
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FASTA

816

91,395

        10         20         30         40         50         60 
MGDTVVEPAP LKPTSEPTSG PPGNNGGSLL SVITEGVGEL SVIDPEVAQK ACQDVLEKVK 

        70         80         90        100        110        120 
LLHGGMAVSS KGAPLELVNG DGVDSEIRCL DDPPAQIREE EDEMGATVAS GTAKGARRRR 

       130        140        150        160        170        180 
QNNSAKQSWL LRLFESKLFD ISMAISYLYN SKEPGVQAYI GNRLFCFRNE DVDFYLPQLL 

       190        200        210        220        230        240 
NMYIHMDEDV GDAIKPYIVY RCRQSINFSL QCALLLGAYS SDMHISTQRH SRGTKLRKLI 

       250        260        270        280        290        300 
LSDELKPAHR KRELPSLSPA PDTGLSPSKR THQRSKSDAT ASISLSSNLK RTASNPKVEN 

       310        320        330        340        350        360 
EDEELSSSTE SIDNSFSSPV RLAPEREFIK SLMAIGKRLA TLPTKEQKTQ RLISELSLLN 

       370        380        390        400        410        420 
HKLPARVWLP TAGFDHHVVR VPHTQAVVLN SKDKAPYLIY VEVLECENFD TTSVPARIPE 

       430        440        450        460        470        480 
NRIRSTRSVE NLPECGITHE QRAGSFSTVP NYDNDDEAWS VDDIGELQVE LPEVHTNSCD 

       490        500        510        520        530        540 
NISQFSVDSI TSQESKEPVF IAAGDIRRRL SEQLAHTPTA FKRDPEDPSA VALKEPWQEK 

       550        560        570        580        590        600 
VRRIREGSPY GHLPNWRLLS VIVKCGDDLR QELLAFQVLK QLQSIWEQER VPLWIKPYKI 

       610        620        630        640        650        660 
LVISADSGMI EPVVNAVSIH QVKKQSQLSL LDYFLQEHGS YTTEAFLSAQ RNFVQSCAGY 

       670        680        690        700        710        720 
CLVCYLLQVK DRHNGNILLD AEGHIIHIDF GFILSSSPRN LGFETSAFKL TTEFVDVMGG 

       730        740        750        760        770        780 
LDGDMFNYYK MLMLQGLIAA RKHMDKVVQI VEIMQQGSQL PCFHGSSTIR NLKERFHMSM 

       790        800        810 
TEEQLQLLVE QMVDGSMRSI TTKLYDGFQY LTNGIM

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References

[1]

"NISC comparative sequencing initiative."
Antonellis A., Benjamin B., Blakesley R.W., Bouffard G.G., Brinkley C., Brooks S., Chu G., Chub I., Coleman H., Fuksenko T., Gestole M., Gregory M., Guan X., Gupta J., Gurson N., Han E., Han J., Hansen N.

Green E.D.
Submitted (MAR-2008) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

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Cross-references

Sequence databases
	DP000634 Genomic DNA. Translation: ACA57933.1.
3D structure databases
ModBase	Search...
Family and domain databases
InterPro	IPR000403. PI3/4_kinase_cat. IPR018936. PI3/4_kinase_CS. IPR015433. PI_Kinase. [Graphical view]
Gene3D	G3DSA:1.10.1070.11. PI3/4_kinase_cat. 1 hit.
PANTHER	PTHR10048. PI_Kinase. 1 hit.
Pfam	PF00454. PI3_PI4_kinase. 1 hit. [Graphical view]
SMART	SM00146. PI3Kc. 1 hit. [Graphical view]
PROSITE	PS00915. PI3_4_KINASE_1. 1 hit. PS00916. PI3_4_KINASE_2. 1 hit. PS50290. PI3_4_KINASE_3. 1 hit. [Graphical view]
ProtoNet	Search...

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Entry information

Entry name

PI4KB_CALMO

Accession

Primary (citable) accession number: B1MTG7

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 3, 2009
Last sequence update:	April 29, 2008
Last modified:	October 13, 2009
This is version 11 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

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Relevant documents

SIMILARITY comments

Index of protein domains and families

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Sequences

References

Cross-references

Sequence databases

3D structure databases

Family and domain databases

Entry information

Relevant documents