B1MLZ4 (NTPA_MYCA9) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Non-canonical purine NTP pyrophosphatase
EC=3.6.1.19

Alternative name(s):
Non-standard purine NTP pyrophosphatase
Nucleoside-triphosphate diphosphatase
Nucleoside-triphosphate pyrophosphatase
Short name=NTPase

Gene names

Ordered Locus Names:

MAB_1485

Organism

Mycobacterium abscessus (strain ATCC 19977 / DSM 44196) [Complete proteome] [HAMAP]

Taxonomic identifier

561007 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium abscessus

Protein attributes

Sequence length	206 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Pyrophosphatase that hydrolyzes non-canonical purine nucleotides such as XTP and ITP/dITP to their respective monophosphate derivatives. Might exclude non-canonical purines from DNA precursor pool, thus preventing their incorporation into DNA and avoiding chromosomal lesions By similarity. HAMAP MF_01405
Catalytic activity	A nucleoside triphosphate + H2O = a nucleotide + diphosphate. HAMAP MF_01405
Cofactor	Binds 1 divalent metal cation ion per subunit; can use either magnesium or manganese By similarity.
Subunit structure	Homodimer By similarity. HAMAP MF_01405
Sequence similarities	Belongs to the HAM1 NTPase family.

Ontologies

Keywords
Biological process	Nucleotide metabolism
Ligand	Magnesium Manganese Metal-binding Nucleotide-binding
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	nucleotide metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW nucleoside-triphosphatase activity Inferred from electronic annotation. Source: InterPro nucleoside-triphosphate diphosphatase activity Inferred from electronic annotation. Source: EC nucleotide binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 206	206	Non-canonical purine NTP pyrophosphatase HAMAP MF_01405		PRO_1000145495
Regions
	Region	7 – 12	6	Substrate binding By similarity
	Region	72 – 73	2	Substrate binding By similarity
Sites
	Metal binding	72	1	Magnesium or manganese By similarity
	Binding site	158	1	Substrate By similarity
	Binding site	178	1	Substrate By similarity
	Binding site	184	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	B1MLZ4 [UniParc]. Last modified April 29, 2008. Version 1. Checksum: DF305C24B141B9A8	FASTA	206	21,791
	10 20 30 40 50 60 MKILVASRNP KKLAELSRVL ESSGVSGVEL VSLTDVPEYE EVPETGASFE DNALIKAREG 70 80 90 100 110 120 VKHTGLACVA DDSGLAVDAL NWMPGVLSAR WSGRHGDDAA NTALLLAQLS DIPDERRGAA 130 140 150 160 170 180 FVSACALVTP EGEEVVVEGR WKGSIARIPA GQNGFGYDPI FVPRGGLRTA AELTPEEKDA 190 200 VSHRGRALAA LLPMLRNLVN LGRTAP

References

[1]

"Acquisition of foreign virulence genes by the cystic fibrosis pathogen Mycobacterium abscessus." Genoscope Ripoll F., Pasek S., Schenowitz C., Dossat C., Barbe V., Rottman M., Heym B., Herrmann J.L., Daffe M., Brosch R., Risler J.L., Gaillard J.L. Submitted (MAR-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 19977 / DSM 44196.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CU458896 Genomic DNA. Translation: CAM61571.1.
RefSeq	YP_001702225.1. NC_010397.1.
3D structure databases
ProteinModelPortal	B1MLZ4.
ModBase	Search...
Protein-protein interaction databases
STRING	B1MLZ4.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBMYCT00000007712; EBMYCP00000007651; EBMYCG00000007710.
GeneID	5964006.
GenomeReviews	Gene locus MAB_1485 in contig CU458896_GR.
KEGG	mab:MAB_1485.
PATRIC	17974663. VBIMycAbs55940_1522.
Organism-specific databases
CMR	Search...
Phylogenomic databases
GeneTree	EBGT00050000017421.
HOGENOM	HBG697237.
OMA	GVYTADW.
Enzyme and pathway databases
BioCyc	MABS561007:MAB_1485-MONOMER.
Family and domain databases
HAMAP	MF_01405. Non_canon_purine_NTPase. [Tree]
InterPro	IPR002637. Ham1p-like. IPR020922. Nucleoside-triphosphatase. [Graphical view]
KO	K02428.
PANTHER	PTHR11067. Ham1p_like. 1 hit.
Pfam	PF01725. Ham1p_like. 1 hit. [Graphical view]
TIGRFAMs	TIGR00042. TIGR00042. 1 hit.
ProtoNet	Search...

Entry information

Entry name

NTPA_MYCA9

Accession

Primary (citable) accession number: B1MLZ4

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	April 29, 2008
Last modified:	January 25, 2012
This is version 27 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program