B1MLU6 (KHSE_MYCA9) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Homoserine kinase
Short name=HK
Short name=HSK
EC=2.7.1.39

Gene names

Name:	thrB
Ordered Locus Names:	MAB_1437

Organism

Mycobacterium abscessus (strain ATCC 19977 / DSM 44196) [Complete proteome] [HAMAP]

Taxonomic identifier

561007 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium abscessus

Protein attributes

Sequence length	306 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the ATP-dependent phosphorylation of L-homoserine to L-homoserine phosphate By similarity. HAMAP MF_00384
Catalytic activity	ATP + L-homoserine = ADP + O-phospho-L-homoserine. HAMAP MF_00384
Pathway	Amino-acid biosynthesis; L-threonine biosynthesis; L-threonine from L-aspartate: step 4/5. HAMAP MF_00384
Subcellular location	Cytoplasm Potential HAMAP MF_00384.
Sequence similarities	Belongs to the GHMP kinase family. Homoserine kinase subfamily.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Threonine biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	threonine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW homoserine kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 306	306	Homoserine kinase HAMAP MF_00384		PRO_1000122432
Regions
	Nucleotide binding	95 – 105	11	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	B1MLU6 [UniParc]. Last modified April 29, 2008. Version 1. Checksum: 06DFF6F863202FB2	FASTA	306	30,847
	10 20 30 40 50 60 MSEVLPAGLA TTVLVPASSA NLGPGFDSLG IALSLYDEIE VNTTESGLKV AVEGQGAGEV 70 80 90 100 110 120 PLDGSHLVVR AIERGLAAGG AAAPGLIVQC HNKIPHSRGL GSSAAAAVAG LGVANGLLAK 130 140 150 160 170 180 AGRAVLSDDV LVQLASEFEG HPDNAAASVL GGAVVSWSET SGATPIYAAT RLDVHPDIKI 190 200 210 220 230 240 VAAIPEEQSS TAHTRVLLPQ AVTHVDARFN ISRVALLTVA LTARPDLLMT ATEDRLHQPQ 250 260 270 280 290 300 RASAMPASAD VLAYLRSQGV AAVLSGAGPA VLALTTVDLP DSAVKYAEDQ GFSLVAMAVS AGVSVR

References

[1]

"Acquisition of foreign virulence genes by the cystic fibrosis pathogen Mycobacterium abscessus." Genoscope Ripoll F., Pasek S., Schenowitz C., Dossat C., Barbe V., Rottman M., Heym B., Herrmann J.L., Daffe M., Brosch R., Risler J.L., Gaillard J.L. Submitted (MAR-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 19977 / DSM 44196.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CU458896 Genomic DNA. Translation: CAM61523.1.
RefSeq	YP_001702177.1. NC_010397.1.
3D structure databases
ProteinModelPortal	B1MLU6.
ModBase	Search...
Protein-protein interaction databases
STRING	B1MLU6.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBMYCT00000006107; EBMYCP00000006046; EBMYCG00000006105.
GeneID	5963959.
GenomeReviews	Gene locus MAB_1437 in contig CU458896_GR.
KEGG	mab:MAB_1437.
PATRIC	17974561. VBIMycAbs55940_1474.
Organism-specific databases
CMR	Search...
Phylogenomic databases
GeneTree	EBGT00050000016145.
HOGENOM	HBG646290.
OMA	DRLHQPY.
ProtClustDB	PRK01212.
Enzyme and pathway databases
BioCyc	MABS561007:MAB_1437-MONOMER.
Family and domain databases
HAMAP	MF_00384. Homoser_kinase. [Tree]
InterPro	IPR006204. GHMP_kinase. IPR006203. GHMP_knse_ATP-bd_CS. IPR000870. Homoserine_kinase. IPR020568. Ribosomal_S5_D2-typ_fold. IPR014721. Ribosomal_S5_D2-typ_fold_subgr. [Graphical view]
Gene3D	G3DSA:3.30.230.10. Ribosomal_S5_D2-type_fold. 1 hit.
KO	K00872.
Pfam	PF00288. GHMP_kinases_N. 1 hit. [Graphical view]
PIRSF	PIRSF000676. Homoser_kin. 1 hit.
PRINTS	PR00958. HOMSERKINASE.
SUPFAM	SSF54211. Ribosomal_S5_D2-typ_fold. 1 hit.
TIGRFAMs	TIGR00191. ThrB. 1 hit.
PROSITE	PS00627. GHMP_KINASES_ATP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KHSE_MYCA9

Accession

Primary (citable) accession number: B1MLU6

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	April 29, 2008
Last modified:	January 25, 2012
This is version 32 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program