B1MKW9 (B1MKW9_MYCA9) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
Dihydroxy-acid dehydratase 1 HAMAP MF_00012
Short name=DAD 1 HAMAP MF_00012
EC=4.2.1.9 HAMAP MF_00012

Gene names

Name:	ilvD1 HAMAP MF_00012
Ordered Locus Names:	MAB_4545

Organism

Mycobacterium abscessus (strain ATCC 19977 / DSM 44196) [Complete proteome] [HAMAP]

Taxonomic identifier

561007 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium abscessus

Protein attributes

Sequence length	578 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	2,3-dihydroxy-3-methylbutanoate = 3-methyl-2-oxobutanoate + H2O. HAMAP MF_00012
Cofactor	Binds 1 4Fe-4S cluster By similarity. HAMAP MF_00012
Pathway	Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 3/4. HAMAP MF_00012 Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 3/4. HAMAP MF_00012
Sequence similarities	Belongs to the IlvD/Edd family. HAMAP MF_00012

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Branched-chain amino acid biosynthesis HAMAP MF_00012
Ligand	4Fe-4S HAMAP MF_00012 Iron Iron-sulfur Metal-binding
Molecular function	Lyase HAMAP MF_00012
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	isoleucine biosynthetic process Inferred from electronic annotation. Source: HAMAP valine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW dihydroxy-acid dehydratase activity Inferred from electronic annotation. Source: HAMAP metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Sites
	Metal binding	140	1	Iron-sulfur (4Fe-4S) By similarity HAMAP MF_00012
	Metal binding	217	1	Iron-sulfur (4Fe-4S) By similarity HAMAP MF_00012

Sequences

	Sequence		Length	Mass (Da)
	B1MKW9 [UniParc]. Last modified April 29, 2008. Version 1. Checksum: 75110358AF6882ED	FASTA	578	60,694
	10 20 30 40 50 60 MSAPHKPDSL RASGSSQPDI KPRSRDVTDG LEKTAARGML RAVGMGDDDW VKPQIGVGSS 70 80 90 100 110 120 WNEITPCNMS LQRLAHSVKD GVHEAGGYPL EFGTISVSDG ISMGHEGMHF SLVSREVIAD 130 140 150 160 170 180 SVETVMQAER LDGSVLLAGC DKSIPGMLMA AARLDLASVF LYNGSIMPGK AKLTDGTEKE 190 200 210 220 230 240 VTIIDAFEAV GACARGLMSR EDVDIIERAI CPGEGACGGM YTANTMASAA EALGMSLPGS 250 260 270 280 290 300 ASPVAIDKRR EEYARKSGEA VVEMLRRGIT ARDILTKEAF ENAIAVVMAF GGSTNAVLHL 310 320 330 340 350 360 LAIAKEAEVE LSLADFTRIG NKVPHLADVK PFGRHVMKDV DEIGGVPVVM RALLDAGLLH 370 380 390 400 410 420 GDCLTVTGKT MAENLAHIAP PDPDGKVLRA MNDPIHPTGG ITILHGSLAP EGAVVKSAGF 430 440 450 460 470 480 ESDVFEGTAR VFERERAALD ALEDGTITHG DVVVIRYEGP KGGPGMREML AITGAIKGAG 490 500 510 520 530 540 LGKDVLLMTD GRFSGGTTGL CVGHIAPEAV DGGPIAFVRD GDRIRLDVAN GKLDLLVDEA 550 560 570 EIAERRKGFE PLPPRYKTGV LAKYTKLVQS AAVGAVCG

References

[1]

"Acquisition of foreign virulence genes by the cystic fibrosis pathogen Mycobacterium abscessus." Genoscope Ripoll F., Pasek S., Schenowitz C., Dossat C., Barbe V., Rottman M., Heym B., Herrmann J.L., Daffe M., Brosch R., Risler J.L., Gaillard J.L. Submitted (MAR-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CU458896 Genomic DNA. Translation: CAM64614.1.
RefSeq	YP_001705268.1. NC_010397.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	B1MKW9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBMYCT00000007780; EBMYCP00000007719; EBMYCG00000007778.
GeneID	5967003.
GenomeReviews	Gene locus MAB_4545 in contig CU458896_GR.
KEGG	mab:MAB_4545.
PATRIC	17980908. VBIMycAbs55940_4618.
Organism-specific databases
CMR	Search...
Phylogenomic databases
GeneTree	EBGT00050000016366.
HOGENOM	HBG671001.
OMA	NMPGAMI.
ProtClustDB	PRK00911.
Family and domain databases
HAMAP	MF_00012. IlvD. [Tree]
InterPro	IPR015928. Aconitase/3IPM_dehydase_swvl. IPR004404. DihydroxyA_deHydtase. IPR000581. DiOHA_6PGluconate_deHydtase. IPR020558. DiOHA_6PGluconate_deHydtase_CS. [Graphical view]
KO	K01687.
PANTHER	PTHR21000. ILVD_EDD_family. 1 hit.
Pfam	PF00920. ILVD_EDD. 1 hit. [Graphical view]
SUPFAM	SSF52016. Aconitase/3IPM_dehydase_swvl. 1 hit.
TIGRFAMs	TIGR00110. IlvD. 1 hit.
PROSITE	PS00886. ILVD_EDD_1. 1 hit. PS00887. ILVD_EDD_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

B1MKW9_MYCA9

Accession

Primary (citable) accession number: B1MKW9

Entry history

Integrated into UniProtKB/TrEMBL:	April 29, 2008
Last sequence update:	April 29, 2008
Last modified:	December 14, 2011
This is version 30 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)