B1MKD5 (B1MKD5_MYCA9) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
4-diphosphocytidyl-2-C-methyl-D-erythritol kinase HAMAP MF_00061
Short name=CMK HAMAP MF_00061
EC=2.7.1.148 HAMAP MF_00061

Alternative name(s):
4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinase HAMAP MF_00061

Gene names

Name:	ispE HAMAP MF_00061
Ordered Locus Names:	MAB_1139

Organism

Mycobacterium abscessus (strain ATCC 19977 / DSM 44196) [Complete proteome] [HAMAP]

Taxonomic identifier

561007 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium abscessus

Protein attributes

Sequence length	314 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol By similarity. HAMAP MF_00061
Catalytic activity	ATP + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol = ADP + 2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol. HAMAP MF_00061
Pathway	Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 3/6. HAMAP MF_00061
Sequence similarities	Belongs to the GHMP kinase family. IspE subfamily. HAMAP MF_00061

Ontologies

Keywords
Biological process	Isoprene biosynthesis HAMAP MF_00061
Ligand	ATP-binding HAMAP MF_00061 RuleBase RU003563 Nucleotide-binding
Molecular function	Kinase HAMAP MF_00061 RuleBase RU003563 EMBL CAM61227.1 Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	terpenoid biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase activity Inferred from electronic annotation. Source: HAMAP ATP binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Regions
	Nucleotide binding	106 – 116	11	ATP By similarity HAMAP MF_00061
Sites
	Active site	21	1	By similarity HAMAP MF_00061
	Active site	148	1	By similarity HAMAP MF_00061

Sequences

	Sequence		Length	Mass (Da)
	B1MKD5 [UniParc]. Last modified April 29, 2008. Version 1. Checksum: 7F2C35ECF2304047	FASTA	314	32,412
	10 20 30 40 50 60 MSETVSDWVP TGAVTVRAPG KVNLYLAVGD LRDDGYHELT TVFHAVSLAD DVTVRDADVL 70 80 90 100 110 120 SIDVVGQGEG TVPTDERNLA WQAAELFADH VGRAPDVSIF INKDIPVAGG MAGGSADAAA 130 140 150 160 170 180 VLVAMNELWH AGVPRRDLHH LAAQLGSDVP FALHGGTALG TGRGEQLATV LARNVFHWVF 190 200 210 220 230 240 AFADGGLATP QVFKEIDRLR ENGDPPRLAE ADELLGALAA GDARRLAPLL GNELQAAAVS 250 260 270 280 290 300 LNPELRRTLR AGESAGALAG IVSGSGPTCA FLCTSADDAV QVSAELAGAG VCRTVRVASG 310 PVHGAQVIQG RSDG

References

[1]

"Acquisition of foreign virulence genes by the cystic fibrosis pathogen Mycobacterium abscessus." Genoscope Ripoll F., Pasek S., Schenowitz C., Dossat C., Barbe V., Rottman M., Heym B., Herrmann J.L., Daffe M., Brosch R., Risler J.L., Gaillard J.L. Submitted (MAR-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CU458896 Genomic DNA. Translation: CAM61227.1.
RefSeq	YP_001701881.1. NC_010397.1.
3D structure databases
ProteinModelPortal	B1MKD5.
ModBase	Search...
Protein-protein interaction databases
STRING	B1MKD5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBMYCT00000004201; EBMYCP00000004140; EBMYCG00000004199.
GeneID	5963664.
GenomeReviews	Gene locus MAB_1139 in contig CU458896_GR.
KEGG	mab:MAB_1139.
PATRIC	17973957. VBIMycAbs55940_1175.
Organism-specific databases
CMR	Search...
Phylogenomic databases
GeneTree	EBGT00050000016146.
HOGENOM	HBG734593.
OMA	KINLHLG.
ProtClustDB	PRK03188.
Family and domain databases
HAMAP	MF_00061. IspE. [Tree]
InterPro	IPR006204. GHMP_kinase. IPR013750. GHMP_kinase_C. IPR004424. IspE. IPR020568. Ribosomal_S5_D2-typ_fold. IPR014721. Ribosomal_S5_D2-typ_fold_subgr. [Graphical view]
Gene3D	G3DSA:3.30.230.10. Ribosomal_S5_D2-type_fold. 1 hit.
KO	K00919.
PANTHER	PTHR20861:SF2. IspE. 1 hit.
Pfam	PF08544. GHMP_kinases_C. 1 hit. PF00288. GHMP_kinases_N. 1 hit. [Graphical view]
PIRSF	PIRSF010376. IspE. 1 hit.
SUPFAM	SSF54211. Ribosomal_S5_D2-typ_fold. 1 hit.
TIGRFAMs	TIGR00154. IspE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

B1MKD5_MYCA9

Accession

Primary (citable) accession number: B1MKD5

Entry history

Integrated into UniProtKB/TrEMBL:	April 29, 2008
Last sequence update:	April 29, 2008
Last modified:	December 14, 2011
This is version 31 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)