B1MHW4 (PUR7_MYCA9) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphoribosylaminoimidazole-succinocarboxamide synthase
EC=6.3.2.6

Alternative name(s):
SAICAR synthetase

Gene names

Name:	purC
Ordered Locus Names:	MAB_0689

Organism

Mycobacterium abscessus (strain ATCC 19977 / DSM 44196) [Complete proteome] [HAMAP]

Taxonomic identifier

561007 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium abscessus

Protein attributes

Sequence length	297 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	ATP + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate + L-aspartate = ADP + phosphate + (S)-2-(5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamido)succinate. HAMAP MF_00137
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide from 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate: step 1/2. HAMAP MF_00137
Sequence similarities	Belongs to the SAICAR synthetase family.

Ontologies

Keywords
Biological process	Purine biosynthesis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological process	purine nucleotide biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoribosylaminoimidazolesuccinocarboxamide synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 297

297

Phosphoribosylaminoimidazole-succinocarboxamide synthase HAMAP MF_00137

PRO_1000095995

Secondary structure

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297

Helix Strand Turn

Details...

Helix

5 – 7

3

Beta strand

8 – 12

5

Beta strand

17 – 21

5

Beta strand

23 – 31

9

Helix

52 – 60

9

Helix

81 – 83

3

Beta strand

87 – 91

5

Beta strand

99 – 103

5

Helix

112 – 116

5

Helix

157 – 160

4

Helix

167 – 189

23

Beta strand

193 – 199

7

Turn

218 – 220

3

Beta strand

221 – 224

4

Helix

226 – 228

3

Turn

238 – 240

3

Helix

241 – 247

7

Helix

267 – 284

18

Helix

289 – 291

3

Sequences

	Sequence		Length	Mass (Da)
	B1MHW4 [UniParc]. Last modified April 29, 2008. Version 1. Checksum: 19FA3755DD47AA6A	FASTA	297	32,730
	10 20 30 40 50 60 MRPSLSDYQH VASGKVRELY RVDDEHLLFV ATDRISAFDF VLDTPIPDKG RILTAMSVFF 70 80 90 100 110 120 FGLLTVPNHL AGPPDDPRIP EEVLGRALLV RRLDMLPVEC VARGYLTGSG LLDYQRTGAV 130 140 150 160 170 180 CGHVLPQGLG EASRLDPPLF TPATKADIGE HDMNVDFAAV VGLVGAVRAN QLRDETIKIY 190 200 210 220 230 240 TRAAAHALHK GIILADTKFE FGVDIEGNLV LADEVFTPDS SRYWDAAHYQ PGVVQDSFDK 250 260 270 280 290 QFVRNWLTGP ESGWDRASDT PPPPLPDEVA VATRERYIEA YERISGLSFS DWIGPSA

References

[1]

"Acquisition of foreign virulence genes by the cystic fibrosis pathogen Mycobacterium abscessus." Genoscope Ripoll F., Pasek S., Schenowitz C., Dossat C., Barbe V., Rottman M., Heym B., Herrmann J.L., Daffe M., Brosch R., Risler J.L., Gaillard J.L. Submitted (MAR-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 19977 / DSM 44196.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

CU458896 Genomic DNA. Translation: CAM60786.1.

RefSeq

YP_001701440.1. NC_010397.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3R9R	X-ray	1.85	A	1-297	[»]

ProteinModelPortal

B1MHW4.

ModBase

Search...

Protein-protein interaction databases

STRING

B1MHW4.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

EBMYCT00000005503; EBMYCP00000005442; EBMYCG00000005501.

GeneID

5963224.

GenomeReviews

Gene locus MAB_0689 in contig CU458896_GR.

KEGG

mab:MAB_0689.

PATRIC

17973053. VBIMycAbs55940_0729.

Organism-specific databases

CMR

Search...

Phylogenomic databases

GeneTree

EBGT00050000017539.

HOGENOM

HBG555040.

OMA

LTLMDEV.

ProtClustDB

PRK13961.

Enzyme and pathway databases

BioCyc

MABS561007:MAB_0689-MONOMER.

Family and domain databases

HAMAP

MF_00137. SAICAR_synth.
[Tree]

InterPro

IPR013816. ATP_grasp_subdomain_2.
IPR001636. SAICAR_synth.
IPR018236. SAICAR_synthetase_CS.
[Graphical view]

Gene3D

G3DSA:3.30.470.20. ATP_grasp_subdomain_2. 1 hit.

KO

K01923.

PANTHER

PTHR11609. SAICAR_synt. 1 hit.

Pfam

PF01259. SAICAR_synt. 1 hit.
[Graphical view]

TIGRFAMs

TIGR00081. PurC. 1 hit.

PROSITE

PS01057. SAICAR_SYNTHETASE_1. 1 hit.
PS01058. SAICAR_SYNTHETASE_2. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

PUR7_MYCA9

Accession

Primary (citable) accession number: B1MHW4

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	April 29, 2008
Last modified:	January 25, 2012
This is version 29 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program