B1MHH1 (GSA_MYCA9) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutamate-1-semialdehyde 2,1-aminomutase
Short name=GSA
EC=5.4.3.8

Alternative name(s):
Glutamate-1-semialdehyde aminotransferase
Short name=GSA-AT

Gene names

Name:	hemL
Ordered Locus Names:	MAB_3978c

Organism

Mycobacterium abscessus (strain ATCC 19977 / DSM 44196) [Complete proteome] [HAMAP]

Taxonomic identifier

561007 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Actinobacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium chelonae group › Mycobacterium abscessus subgroup › Mycobacterium abscessus

Protein attributes

Sequence length	446 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	(S)-4-amino-5-oxopentanoate = 5-aminolevulinate. HAMAP-Rule MF_00375
Cofactor	Pyridoxal phosphate By similarity.
Pathway	Porphyrin metabolism; protoporphyrin-IX biosynthesis; 5-aminolevulinate from L-glutamyl-tRNA(Glu): step 2/2. HAMAP-Rule MF_00375
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm Potential HAMAP-Rule MF_00375.
Sequence similarities	Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family. HemL subfamily.

Ontologies

Keywords
Biological process	Porphyrin biosynthesis
Cellular component	Cytoplasm
Ligand	Pyridoxal phosphate
Molecular function	Isomerase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	chlorophyll biosynthetic process Inferred from electronic annotation. Source: HAMAP protoporphyrinogen IX biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	glutamate-1-semialdehyde 2,1-aminomutase activity Inferred from electronic annotation. Source: HAMAP pyridoxal phosphate binding Inferred from electronic annotation. Source: InterPro transaminase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 446	446	Glutamate-1-semialdehyde 2,1-aminomutase HAMAP-Rule MF_00375		PRO_0000382344
Amino acid modifications
	Modified residue	281	1	N6-(pyridoxal phosphate)lysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	B1MHH1 [UniParc]. Last modified April 29, 2008. Version 1. Checksum: 29D8BB3F6CEB6147	FASTA	446	46,259
	10 20 30 40 50 60 MDDLASAETL DAVSDETPNS ARLFADASTV IPGGVNSPVR AFAAVGGVPR FVRSGAGCWL 70 80 90 100 110 120 TDVDDNRYVD LVCSWGPLIL GHAHPAVVEA VQRAAKDGLS YGAPVEGEIE LAREIIDRVQ 130 140 150 160 170 180 PVEKVRLVNS GTEATMSAVR LARGFTGRPK IVKFAGCYHG HADALLADAG SGLATFGLPS 190 200 210 220 230 240 SPGVTGASAA DTIVLPYNDI GAVAQVFAEQ GDHIAAVITE AAAGNMGTVA PGPDFNAGLR 250 260 270 280 290 300 RITENHGALL IMDEVMTGFR VSRSGWYGID QVAGDLVTFG KVMSGGMPAA AFGGRADIMD 310 320 330 340 350 360 RLAPLGPVYQ AGTLSGNPVA VAAGLASLRA ADAKAYETLD RNADALIALL TASLDAEGVA 370 380 390 400 410 420 HHIGRAGNMF SVFFGTEPVR DFQSAKATET WRYPAFFHGL LSRGVYPPAS AFETWFVSTA 430 440 LDDEAFDRIA DALPAAARAA AAAEEN

References

[1]

"Acquisition of foreign virulence genes by the cystic fibrosis pathogen Mycobacterium abscessus." Genoscope Ripoll F., Pasek S., Schenowitz C., Dossat C., Barbe V., Rottman M., Heym B., Herrmann J.L., Daffe M., Brosch R., Risler J.L., Gaillard J.L. Submitted (MAR-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 19977 / DSM 44196.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CU458896 Genomic DNA. Translation: CAM64052.1.
RefSeq	YP_001704706.1. NC_010397.1.
3D structure databases
ProteinModelPortal	B1MHH1.
SMR	B1MHH1. Positions 16-435.
ModBase	Search...
Protein-protein interaction databases
STRING	561007.MAB_3978c.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	CAM64052; CAM64052; MAB_3978c.
GeneID	5966441.
KEGG	mab:MAB_3978c.
PATRIC	17979774. VBIMycAbs55940_4052.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0001.
HOGENOM	HOG000020210.
KO	K01845.
OMA	FNGNPIS.
Enzyme and pathway databases
BioCyc	MABS561007:GJTG-3998-MONOMER.
UniPathway	UPA00251; UER00317.
Family and domain databases
Gene3D	3.40.640.10. 1 hit. 3.90.1150.10. 2 hits.
HAMAP	MF_00375. HemL_aminotrans_3.
InterPro	IPR004639. 4pyrrol_synth_GluAld_NH2Trfase. IPR005814. Aminotrans_3. IPR015424. PyrdxlP-dep_Trfase. IPR015421. PyrdxlP-dep_Trfase_major_sub1. IPR015422. PyrdxlP-dep_Trfase_major_sub2. [Graphical view]
PANTHER	PTHR11986. PTHR11986. 1 hit. PTHR11986:SF5. PTHR11986:SF5. 1 hit.
Pfam	PF00202. Aminotran_3. 1 hit. [Graphical view]
SUPFAM	SSF53383. PyrdxlP-dep_Trfase_major. 1 hit.
TIGRFAMs	TIGR00713. hemL. 1 hit.
PROSITE	PS00600. AA_TRANSFER_CLASS_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GSA_MYCA9

Accession

Primary (citable) accession number: B1MHH1

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 1, 2009
Last sequence update:	April 29, 2008
Last modified:	May 1, 2013
This is version 45 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program